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Papanicolaou staining kit and staining method thereof

A technique of Papanicolaou staining and reagent kit, which is applied in the field of in vitro diagnosis, and can solve problems such as difficulty in mastering, complicated procedures, and long staining time

Active Publication Date: 2019-01-11
SICHUAN MACCURA BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Traditional Papanicolaou staining procedures are complicated, the staining time is long and difficult to master, and the need for separate transparency increases the time and operation steps

Method used

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  • Papanicolaou staining kit and staining method thereof
  • Papanicolaou staining kit and staining method thereof
  • Papanicolaou staining kit and staining method thereof

Examples

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Comparison scheme
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Embodiment 1

[0064] A Papanicolaou staining kit, the kit includes a hematoxylin staining solution, a blue-returning solution, an eosin staining solution and a cleaning solution. The specific formula is shown in Table 1.

[0065] Table 1 is the Papanicolaou staining kit formula of embodiment 1

[0066]

Embodiment 2

[0068] A Papanicolaou staining kit, the kit includes a hematoxylin staining solution, a blue-returning solution, an eosin staining solution and a cleaning solution. The specific formula is shown in Table 2.

[0069] Table 2 is the Papanicolaou staining kit formula of embodiment 2

[0070]

Embodiment 3

[0072] A Papanicolaou staining kit, the kit includes a hematoxylin staining solution, a blue-returning solution, an eosin staining solution and a cleaning solution. The specific formula is shown in Table 3.

[0073] Table 3 is the Papanicolaou staining kit formula of embodiment 3

[0074]

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Abstract

The invention discloses a papanicolaou staining kit and a staining method thereof. The kit comprises hematoxylin stain, a back-to-blue liquid, eosin stain and a cleaning solution. The eosin stain is prepared from the following substances in concentration: 600-900ml / L ethanol, 100-400ml / L methanol, 1-4g / L eosin Y, 1-4g / L phosphotungstic acid, 0.01-2g / L fast green and 1-10ml / L weak acid; and the cleaning solution is prepared by mixing solvent oil, absolute ethyl alcohol and propylene glycol methyl ether acetate according to a volume ratio of (10-350):(600-950):(10-200). The papanicolaou stainingkit disclosed by the invention is rapid in staining, the stained colors have clear differences, and the diagnosis efficiency is effectively improved.

Description

technical field [0001] The invention relates to the field of in vitro diagnosis, in particular to a Papanicolaou staining kit and a staining method thereof. Background technique [0002] Papanicolaou staining is the best staining method in the staining of exfoliated cells. This staining method not only has the characteristics of clear nucleus structure, clear color separation, good transparency, and bright cytoplasm, but also the stained specimens are not easy to decolorize and can be stored for a long time. . [0003] At present, cervicitis, tuberculosis, pleurisy, urinary tract infection and other diseases have become common clinical diseases and frequently-occurring diseases in my country. The incidence of these diseases is high and the cure time is long. How to diagnose these diseases has become a research hotspot. Liquid-based thin-layer cytology technology can assist clinicians to detect the above-mentioned diseases early and formulate correct treatment plans, so that...

Claims

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Application Information

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IPC IPC(8): G01N1/30
CPCG01N1/30G01N2001/302
Inventor 刘静赵光波
Owner SICHUAN MACCURA BIOTECH CO LTD
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