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Papanicolaou staining kit and its staining method

A Papanicolaou staining and kit technology, applied in the field of in vitro diagnosis, can solve the problems of complicated procedures, difficulty in mastering, increasing time and operation steps, etc.

Active Publication Date: 2021-05-04
SICHUAN MACCURA BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Traditional Papanicolaou staining procedures are complicated, the staining time is long and difficult to master, and the need for separate transparency increases the time and operation steps

Method used

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  • Papanicolaou staining kit and its staining method
  • Papanicolaou staining kit and its staining method
  • Papanicolaou staining kit and its staining method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0064] A Papanicolaou staining kit, the kit includes a hematoxylin staining solution, a blue-returning solution, an eosin staining solution and a cleaning solution. The specific formula is shown in Table 1.

[0065] Table 1 is the Papanicolaou staining kit formula of embodiment 1

[0066]

Embodiment 2

[0068] A Papanicolaou staining kit, the kit includes a hematoxylin staining solution, a blue-returning solution, an eosin staining solution and a cleaning solution. The specific formula is shown in Table 2.

[0069] Table 2 is the Papanicolaou staining kit formula of embodiment 2

[0070]

Embodiment 3

[0072] A Papanicolaou staining kit, the kit includes a hematoxylin staining solution, a blue-returning solution, an eosin staining solution and a cleaning solution. The specific formula is shown in Table 3.

[0073] Table 3 is the Papanicolaou staining kit formula of embodiment 3

[0074]

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PUM

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Abstract

The invention discloses a Papanicolaou staining kit and a staining method thereof. The kit includes hematoxylin staining solution, bluing solution, eosin staining solution and washing solution. The eosin dye solution is prepared from the following concentrations of substances: 600-900ml / L of ethanol, 100-400mL / L of methanol, 1-4g / L of eosin Y, 1-4g / L of phosphotungsten Acid, the fast green of 0.01-2g / L, the weak acid of 1-10mL / L; Described cleaning solution is made up of mineral spirits, dehydrated alcohol and propylene glycol methyl ether acetate by volume ratio 10-350:600-950:10- 200 mixed. The Papanicolaou staining kit of the invention is used for rapid staining, and the colors are clearly distinguished after staining, thereby effectively improving the efficiency of diagnosis.

Description

technical field [0001] The invention relates to the field of in vitro diagnosis, in particular to a Papanicolaou staining kit and a staining method thereof. Background technique [0002] Papanicolaou staining is the best staining method in the staining of exfoliated cells. This staining method not only has the characteristics of clear nucleus structure, clear color separation, good transparency, and bright cytoplasm, but also the stained specimens are not easy to decolorize and can be stored for a long time. . [0003] At present, cervicitis, tuberculosis, pleurisy, urinary tract infection and other diseases have become common clinical diseases and frequently-occurring diseases in my country. The incidence of these diseases is high and the cure time is long. How to diagnose these diseases has become a research hotspot. Liquid-based thin-layer cytology technology can assist clinicians to detect the above-mentioned diseases early and formulate correct treatment plans, so that...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N1/30
CPCG01N1/30G01N2001/302
Inventor 刘静赵光波
Owner SICHUAN MACCURA BIOTECH CO LTD
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