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Improved he staining method

A dyeing method and hematoxylin technology, applied in the preparation of test samples, etc., can solve the problems of no further discussion on the influence of dyeing, inconsistent heating temperature, and increase dyeing quality, etc., to facilitate the control of dyeing quality, consistent temperature conditions, and dyeing. The effect of shortening time

Active Publication Date: 2019-11-08
SICHUAN MACCURA BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It discloses the method of dewaxing step at 30-50°C and hematoxylin staining and differentiation at 30-40°C, but this method only gives the temperature range and does not further discuss the influence of different temperatures on staining
[0006] However, since HE staining requires cumbersome steps such as dewaxing, rehydration, hematoxylin staining, differentiation, bluing, eosin staining, dehydration, and clearing, only one or two steps are heated up, although the staining is accelerated to a certain extent. , but this increases the overall complexity of the method. There are requirements for heating or non-heating, or inconsistent heating temperatures between the steps, requiring multiple heating devices and larger operating spaces, and also increases the artificiality that causes the dyeing quality to be inconsistent. factor

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0076] Embodiment 1: the influence of different temperatures on HE dyeing effect

[0077] According to the above method 2 (the total process time is 18.5 minutes), 14 groups of experiments were carried out, and the temperature of the water bath in each group of experiments was set at 30°C, 35°C, 37°C, 40°C, 41°C, 42°C, 43°C, 44°C, 45°C, 46°C, 47°C, 48°C, 49°C and 50°C, the electromagnetic stirring speed was fixed at 1000rpm. The results are shown in Table 1 below.

[0078] Table 1: Effect of temperature on dyeing effect

[0079]

[0080]

[0081] It can be seen from the above table that at the same rotating speed (1000rpm), the increase of temperature has no obvious effect on the chip falling. further reference figure 1 , it can be seen that when the temperature is lower than 41°C, the coloring of hematoxylin is lighter (B), and when the temperature is lower than 37°C (A), there is a phenomenon of blurred vision under the high power lens, which is because the dewaxing...

Embodiment 2

[0082] Embodiment 2: the influence of different temperatures on HE dyeing effect

[0083] According to the above method 1 (the total process time is 25.5 minutes), 14 groups of experiments were carried out, and the temperature of the water bath in each group of experiments was set at 30°C, 35°C, 37°C, 40°C, 41°C, 42°C, 43°C, 44°C, 45°C, 46°C, 47°C, 48°C, 49°C and 50°C, the electromagnetic stirring speed was fixed at 1000rpm. The results are shown in Table 2 below.

[0084] Table 2: Effect of temperature on dyeing effect

[0085]

[0086] It can be seen from the above table that the dehydration and clearing steps after eosin dyeing are carried out under heating and stirring conditions to obtain similar effects as in Example 1. That is to say, at the same rotational speed (1000rpm), the increase of temperature has no obvious effect on the falling of slices; when it is lower than 41°C, the hematoxylin coloring is lighter, and when it is lower than 37°C, there is blurred visi...

Embodiment 3

[0087] Embodiment 3: the influence of different rotating speeds on HE dyeing effect

[0088] Five sets of experiments were carried out according to the above-mentioned method 2 (the total process time was 18.5 minutes), in which the temperature of the water bath was fixed at 42°C, and the electromagnetic stirring speed was set at 800rpm, 900rpm, 1000rpm, 1100rpm, and 1200rpm in each set of experiments. The results are shown in Table 3 below.

[0089] Table 3 The influence of rotating speed on the dyeing effect

[0090]

[0091] At the same temperature, the increase of rotational speed has no obvious effect on chip drop. combine figure 2 , it can be seen that when it is lower than 900rpm (A), the coloring of hematoxylin is lighter, and when it is higher than 1100rpm (C), the coloring of hematoxylin is darker. At 42°C, the preferred rotation speed is 900-1100rpm (B), with moderate coloring.

[0092] Exhaustion of the experiment according to Method 1 with an aqueous solut...

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Abstract

The invention discloses an improved HE staining method. The method includes steps of dewaxing, rehydration and hematoxylin staining of sections under electromagnetic stirring at a temperature of 41-44DEG C. The method enables the dewaxing, rehydration and hematoxylin staining steps to be carried out at an elevated temperature and cooperates with the electromagnetic stirring, and so the staining speed is greatly accelerated. Furthermore, the stirring and temperature conditions in the steps are the same, and the steps can be sequentially carried out in the same constant temperature equipment, which is convenient for the operation and is more convenient for the staining quality control and the consistent staining effect.

Description

technical field [0001] The invention relates to a method for staining tissue sections, in particular to a method for hematoxylin-eosin staining. Background technique [0002] Hematoxylin-eosin staining (hematoxylin-eosin staining), referred to as HE staining, is one of the commonly used staining methods in paraffin section technology. The hematoxylin staining solution is alkaline, which mainly makes the chromatin in the nucleus and the nucleic acid in the cytoplasm stain blue-purple; the eosin is an acid dye, which mainly makes the components in the cytoplasm and extracellular matrix stain red. Although the HE dyeing method exists in the prior art, the dyeing time is long. [0003] It is known that increasing the temperature can accelerate the oxidation and coloring speed of hematoxylin. It has been reported that dyeing at a temperature of about 30-40°C can speed up the dyeing speed. However, the dyeing temperature should not be too high, otherwise it will lead to too dee...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N1/30
CPCG01N1/30
Inventor 郑志建杨丽念波廖世玉刘春晖
Owner SICHUAN MACCURA BIOTECH CO LTD
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