PAP staining kit as well as preparation method and staining method thereof

Abstract

The invention relates to the technical field of biology, in particular to a PAP staining kit as well as a preparation method and a staining method thereof. The kit comprises a hematoxylin staining solution, an EA / OG staining solution, a buffer solution and a cleaning solution, wherein the hematoxylin staining solution comprises hematoxylin, sodium iodate, aluminum sulfate, sodium sulfite, ethanol, ethylene glycol, glycerol, acetic acid and water; the pH value of the hematoxylin staining solution is 2.0-3.0; the EA / OG staining solution comprises orange G, phosphotungstic acid, bright green SF light yellow, water-soluble eosin, sodium sulfite, ethanol, ethylene glycol, methanol, isopropanol, acetic acid and water; the pH value of the EA / OG staining solution is 5.0-6.0; and the pH value of the buffer solution is 6.8-7.2. The PAP staining solution is uniform in staining effect, clear in cell structure, bright in cell coloring, easy to clinically observe and diagnose, convenient to prepare, simple in staining step, stable in effect, clear in cell contrast and long in service life.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a Pap staining kit, a preparation method and a staining method thereof. Background technique [0002] Papanicolaou staining is one of the most basic staining methods in cytology clinical examination. It is not only suitable for gynecological cytology smear staining such as screening cervical cancer and precancerous lesions, but also suitable for pleural effusion, ascites, sputum, fine needle aspiration, etc. For the staining of gynecological cell samples, the staining effect is bright, the transparency is good, and the nuclear membrane, nucleolus, and chromatin structure are clear. Cells contain a variety of amino acids with acid-base chemical substances and different charges, which react chemically with the acid-base chemical substances in the Papanicolaou staining solution, and combine with the dyes of different charges in the Papanicolaou staining solution, thereby making the secr...

AI Technical Summary

Problems solved by technology

[0006] 2) In the traditional Papanicolaou staining, the staining solution for staining the cytoplasm usually uses orange G6 and EA50 or EA36, the staining time is long, and the staining procedure is cumbersome

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