Papanicolaou staining fluid and using method thereof

Abstract

The invention relates to Papanicolaou staining fluid and a using method thereof. The Papanicolaou staining fluid consists of fluid A, fluid B and 95 percent ethanol, wherein the fluid A is improved hematoxylin staining fluid; the fluid B is prepared from a phosphotungstic acid, eostm and light green. A method for preparing the improved hematoxylin staining fluid comprises the following steps of dissolving hematoxylin into the 95 percent ethanol, dissolving potassium alum into 100 DEG C boiling water, mixing two mixed solutions, adding an oxidant, stirring for at least 50 minutes, adding a glacial acetic acid, and stirring for at least 50 minutes. A method for preparing the fluid B comprises the following steps of mixing 95 to 97 mass percent of 0.2 to 0.6 percent phosphotungstic acid solution, 1.5 to 2.5 mass percent of eostm solution and 1.5 to 2.5 mass percent of light green solution. When the Papanicolaou staining fluid is used, a smear is fixed in the 95 percent ethanol, and is flushed in a specific environment by adding a corresponding amount of fluid A and a corresponding amount of fluid B. The Papanicolaou staining fluid has the advantages of low cost, bright color, clear contrast, coloring stability, is simple to prepare and convenient to operate, is easily popularized and used in various medical units and the like, and is applied to the display and diagnosis of the estrogen level of a vaginal smear and the poorly-differentiated small-angle cell carcinoma of a cervical smear and a sputum smear.

Description

technical field [0001] The invention belongs to in vitro diagnostic reagents and their usage methods, in particular to a Papanicolaou staining solution for staining exfoliated cells and a usage method thereof. The invention is suitable for displaying and diagnosing small keratinocyte carcinomas with estrogen levels in vaginal smears, cervical smears and sputum smears. Background technique [0002] Papanicolaou staining is the most widely used method for staining exfoliated cells, and it is also a better and more commonly used staining method in pathological sections and exfoliated cells. Its staining reagents include hematoxylin staining solution, orange yellow G6 staining solution, bright green, Bismarck brown, yellow eosin, saturated lithium carbonate, phosphotungstic acid, absolute ethanol, 95% ethanol, 80% ethanol, 70% ethanol, 50 % ethanol, 0.25% or 0.5% hydrochloric acid and other reagents. The results of Papanicolaou staining showed good cell transparency and clear ...

AI Technical Summary

Problems solved by technology

However, most of the Pap staining solutions are prepared with 95% ethanol. There are many types of reagents, high cost, troublesome preparation of the staining solution, cumbersome staining steps, easy aging of the staining solution, unstable staining effect, and difficulty in popularization in primary medical units.