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Mercury-free environment-friendly hematoxylin staining solution

A technology of hematoxylin and staining solution, which is applied in the field of pathological experiments, can solve common problems such as crystallization and solification, and long natural oxidation time, and achieve the effects of avoiding the generation of metal ions, reducing the concentration of ineffective solutes, and clear staining

Inactive Publication Date: 2011-08-31
HARBIN GREEN SPECIMEN TECH DEV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, this treatment solution based on traditional tissue fixation still uses formaldehyde, a toxic reagent that seriously pollutes the environment, and the hematoxylin staining solution used in it uses natural oxidation for a long time.
[0006] In addition, some existing mercury-free hematoxylin staining solutions use potassium iodate or sodium iodate as an oxidizing agent, and the free metal ions produced after the iodate completes the oxidation effect combine with the color element molecules to affect the dyeing ability of the staining solution. In addition, the metal ions Combined with acetate in the staining solution to form insoluble salt crystals, which become crystal nuclei leading to the common crystallization and solification of hematoxylin staining solution

Method used

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  • Mercury-free environment-friendly hematoxylin staining solution
  • Mercury-free environment-friendly hematoxylin staining solution

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Dissolve 30 grams of aluminum sulfate in 1500 ml of deionized water and heat to 60 degrees Celsius. Add 100 ml of 3% hematoxylin anhydrous ethanol solution, mix well, add 60 ml of 0.5% periodic acid aqueous solution and mix well. Move the solution to cold water to cool down to room temperature, then add 15ml of glacial acetic acid and mix well.

Embodiment 2

[0029] Dissolve 120 grams of aluminum sulfate in 2000 ml of deionized water and heat to 100 degrees Celsius. Add 300ml of 10% hematoxylin anhydrous ethanol solution, mix well, add 100ml of 2% periodic acid aqueous solution and mix well. Move the solution into cold water to cool down to room temperature, then add 30ml of glacial acetic acid and mix well.

Embodiment 3

[0031] Dissolve 70 grams of aluminum sulfate in 1775 ml of deionized water and heat to 80 degrees Celsius. Add 200ml of 5% hematoxylin anhydrous ethanol solution, mix well, add 80ml of 1% periodic acid aqueous solution and mix well. Move the solution into cold water to cool down to room temperature, then add 25ml of glacial acetic acid and mix well.

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Abstract

The invention provides a mercury-free environment-friendly hematoxylin staining solution, which comprises the following components in portions by volume: 100-300 portions of 3-10 percent hematoxylin absolute ethyl alcohol solution, 1500-2000 portions of 2-6 percent aluminum sulfate aqueous solution, 60-100 portions of .05-2 percent periodic acid aqueous solution, and 15-30 portions of glacial acetic acid. Non-toxic and innocuous chemical reagents are adopted for the mercury-free environment-friendly hematoxylin staining solution, and a histological section staining experiment shows that the nucleus is clearly stained and the structures of the chromatin, nuclear membrane and nucleolus are clear.

Description

technical field [0001] The invention relates to in vitro diagnostic reagents, in particular to a mercury-free environment-friendly hematoxylin staining solution and its preparation method and application, belonging to the technical field of pathological experiments. Background technique [0002] The birth of histopathology has a history of more than 150 years. In all hospitals, as long as there is an operation, the pathology department of the hospital will make a histopathological diagnosis on the surgical tissue samples. [0003] Conventional paraffin-embedded HE staining is the most basic technique currently used to process tissue samples, it is the basis of pathological diagnostic methodology, and it is also the reference standard for evaluating the advantages and disadvantages of many new methods and technologies. The traditional method is to place the tissue sample in the tank of the treatment solution, pour or transfer in different treatment solutions in turn, and the ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C09B65/00G01N1/30
Inventor 李明王德田董建强
Owner HARBIN GREEN SPECIMEN TECH DEV
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