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CRISPR/Cpf1 plant genome directional modification function unit, carrier comprising function unit, and application thereof

The invention belongs to the technical field of gene engineering, and concretely relates to a CRISPR/Cpf1 plant genome directional modification function unit, a carrier comprising the function unit, and application thereof. The invention aims to build an effective plant genome CRISPR/Cpf1 directional modification framework carrier according to an action principle of a CRISPR/Cpf1 system and a vegetable cell gene expression characteristic, and realize high-efficient application thereof in plant genome directional modification. The technical scheme provided by the invention is characterized by building the CRISPR/Cpf1 plant genome directional modification framework carrier formed by lachnospiraceae bacterium Cpf1 nuclease protein (LbCpf1) expression unit and a guiding crRNA transcription expression unit. The invention also provides a method for designing specificity-guided crRNA and building a CRISPR/Cpf1 recombinant expression vector aiming at rice genome target loci by adopting the CRISPR/Cpf1 plant genome directional modification framework carrier. The invention provides the high-efficient CRISPR/Cpf1 plant directional modification framework carrier, which can effectively realize simple, fast and high-efficient directional modification based on the CRISPR/Cpf1 system aiming at a plant genome target sequence.
Owner:UNIV OF ELECTRONIC SCI & TECH OF CHINA

Apparatus and method for generating panorama images and apparatus and method for object-tracking using the same

Provided are an apparatus and method for generating panorama images and an apparatus and method for tracking an object using the same. The apparatus for generating panorama images estimates regional motion vectors with respect to respective lower regions set in an image frame, determines a frame motion vector by using the regional motion vectors based on a codebook storing sets of normalized regional motion vectors corresponding to a camera's motion and then accumulates motion mediating variables computed with respect to respective continuous image frames to determine an overlapping region, and matches the overlapping regions to generate a panorama image. The apparatus for tracking an object sets the panorama image generated by the apparatus for generating panorama images as a background image, and determines an object region by averaging pixel value difference values between pixels that constitute a captured input image and corresponding pixels in the panorama image. Regional motion vectors are estimated by using the coordinate of a pixel selected in lower regions, and thus a computing process can be simplified and a process speed can be increased. In addition, effects of wrongly estimated regional motion vectors can be minimized by using a codebook. Furthermore, noises are minimized and an object can be accurately extracted by using averaged pixel value difference values.
Owner:CHUNG ANG UNIV IND ACADEMIC COOP FOUND

Computer design method of standard distribution network line

The invention discloses a computer design method of a standard distribution network line. The computer design method of the standard distribution network line comprises the steps of drawing construction diagrams of overhead power distribution lines and cable lines according to distribution line design requirements and a standard diagram, accurately counting materials required by a project, and automatically generating various kinds of report forms. According to the computer design method of the standard distribution network line, the ERP (Enterprise Resource Planning) material entering process can be greatly optimized, and moreover, a preliminary budget of the material part can be automatically embedded into a rural network project preliminary budget template, and the differences between the construction diagram and an as-constructed diagram are analyzed to generate a material balance sheet after the construction diagram and the as-constructed diagram are accomplished, so that a data support is provided for the material recovery, the project auditing and the construction cost, the project materials can be managed and controlled, and create favorable conditions are created for the fixed-assets management, the base data management and the distribution network operation and maintenance management of the post-stage project.
Owner:XUYI POWER SUPPLY OF JIANGSU ELECTRIC POWER +2
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