43 results about "Natural sequence" patented technology

The invention relates to a method for quickly detecting the intensity of four basic tastes (sour, sweet, bitter, and salty) in a liquid food by using an electronic tongue. The method comprises the following steps: preparing an HCl solution with a concentration of 0.01mol / L from distilled water as the activating solution of the electronic tongue (five tastes) sensor; using distilled water of the cleaning solution (pay attention to replacing new distilled water); adding the HCl solution with a concentration of 0.01mol / L as the calibration solution, so that the electronic tongue sensor can be used for analyzing samples after calibration; selecting four kinds of substances with four basic tastes with a concentration suitable for the normal concentration level in the human mouth as the basic samples; determining the number of cleaning beakers according to the number of the samples, wherein each sample is cleaned in the specific cleaning beaker, and the samples are cleaned according to the natural sequence; placing the electronic tongue sensor in each beaker for 120 seconds to detect the taste of the sample, and detecting for six times for each sample to obtain that the taste of the sample has a semi-logarithmic relationship with the response strength on the electronic tongue sensor; and carrying out macro operation by electronic tongue software on the results to obtain the relative intensity relationship between different concentrations of the basic taste substances on the electronic tongue.

The present invention describes the expression cassette for transforming eukaryotic cell which comprises the peptide encoding non-natural sequence of nucleotides with xylose isomerase feature (SEQ ID NO: 1), optionally also comprising other genes of pentose phosphate route. Additionally, it is described the microorganism filed under the number DSM28739, which, in addition to the above-mentioned modifications, also present genetic modifications from adaptive evolution. The described microorganism shows efficient consumption of xylose and conversion of ethanol when compared to its correspondent without said genetic modifications and mutations from evolution. It is also described the process for producing biofuels e biochemicals, preferably ethanol, mainly from the lignocellulosic portion of the vegetal biomass. Biofuels, preferably ethanol, and biochemicals produced by the process of the invention are also described.

One configuration as discussed herein includes a data processor acting as streaming server for providing streaming media from a repository to a decoder. The data processor retrieves reconstruction data and corresponding stream metadata from a repository, the reconstruction data encoded in accordance with a tiered hierarchy including multiple levels of quality. The data processor transmits selected portions of the reconstruction data to one or more decoder resources. The decoder resources reconstruct renditions of portions of a signal such as images / frames based on the transmitted portions of reconstruction data. During the transmission step, the data processor may vary a level of quality of the reconstruction data retrieved and transmitted to the decoder resource. Also, at times the data processor may transmit the levels of quality of the reconstruction data out of the natural sequence of the portions of the signal that they allow to reconstruct, anticipating or postponing the transmission of certain levels of quality of reconstruction data related to specific portions of the signal. The decoder resources decode the received reconstruction data to play back the signal at different levels of quality.

The invention relates to a system for forwarding messages of an ESB. The system comprises an access engine and an ESB kernel, wherein the access engine provides access support of respective technicalspecification for a caller party and a callee party, and forwards a call request of the caller party or an execution result of the callee party; and the ESB kernel determines positions and types of afirst access engine corresponding to the caller party and a second access engine corresponding to the callee party, and selects direct forwarding, forwarding after natural serialization or forwardingor after unified message conversion of the call request transmitted by the caller party through the first access engine according to a determination result. The invention also relates to an ESB message forwarding method and a server which correspond to the system. The method and the system thereof perform message conversion of different strategies aiming at different conditions on comparison of the access engine types on the ESB, execute direct forwarding, natural serialization or unified message conversion of the messages according to different priorities, and achieve the aims of reducing message conversion times and improving the performance through optimization.

The invention relates to a recombinant polypeptide comprising amino acid sequence derived from at least one of an HIV gag gene product; an HIV pol gene product; or an HIV nef gene product, said sequence being mutated with respect to the natural sequence of said gene product, and said sequence maintaining each of the naturally occurring CD8+ T cell epitopes of said gene product as defined in p17 and p24 (gag), amino acids 1-440 of RT (pol) and nef shown in Example 8. Furthermore the invention relates to nucleic acids encoding same, and viral vectors encoding same, and to their use in medicine and in immunisation and vaccination.

Methods, compositions, and systems are provided for characterization of modified nucleic acids. Methods are provided for sequencing hemi-natural nucleic acids such as hemi-genomic DNA, having two complementary strands, one a natural sequence and the other a synthetic sequence. The identification of modified bases can be enhanced by comparing the sequencing information from the natural sequence, which has, for example, natural base modifications, with the synthetic sequence, which typically has no base modifications. The presence and identity of a modified base can be determined by monitoring kinetics, for example the kinetics of polymer meditated nucleic acid synthesis.

The invention provides an I-group 4-type fowl adenovirus DNA vaccine and application thereof. According to the technical scheme, based on experimental means, research finds and shows that fiber protrusions have good immunity prototypes, in this way, with 4-type fowl adenovirus fibrous protein C-terminal genes being antigen substances, codon optimization is carried out on the basis of a natural sequence, an eukaryotic expression vector pCAGGS is cloned, and the DNA vaccine pCAGoptiFAV4C is constructed. According to the researched fowl adenovirus NDA vaccine, a method of gene engineering fermentation is adopted for preparing antigens, and therefore the I-group 4-type fowl adenovirus DNA vaccine is low in cost, pure in antigen and safe to use. By the utilization of a serology method and an immunity virus attack method, the immunity effect of the vaccine is evaluated. The result shows that the vaccine can provide effective immune protection for fowl, and has good commercial development prospects. Compared with a traditional vaccine, the DNA vaccine achieves the safety of subunit vaccines and inactivated vaccines, and also has the features of simultaneous induction of humoral immunity and cellular immune response, wherein the features only belong to attenuated vaccines or recombinant vaccines.

The invention provides a nucleotide sequence suitable for secretory expression of recombinant candida utilis uricase (uricase, EC1.7.3.3) by virtue of escherichia coli, a carrier containing the nucleotide sequence, an engineering cell containing the carrier, and a method for purifying the recombinant candida utilis uricase from the engineering cell. On the basis of a natural sequence of a uricase gene, a codon of the uricase gene is optimized, the transformed uricase gene is cloned to a prokaryotic expression vector pET42a(+); an escherichia coli Rossetta strain is transformed; the expression level can reach 50% of soluble protein of the escherichia coli by IPTG induction; the expressed protein is soluble protein; and the biological activity of the expressed protein can be up to that of 37.89U / mg recombinant protein. The recombinant candida utilis uricase product can be efficiently and simply obtained at low cost. The invention also comprises an application of the recombinant candida utilis uricase prepared by the method in preparation of medicines, foods, health care products and cosmetics for treating hyperuricemia-related diseases or pathological symptoms of a human body.

The peptide library has its every peptide has the general expression of Z-XnXn+1X n+2X n+3X n+4X n+5X n+6X n+7X n+8X n+9-Y, whereX is one of L-configuration natural amino acid residue, n is the natural sequence position of Xn amino acid in the protein sequence and is integral, Y expresses NH2 or OH, and Z is H or acetyl radical Ac. The polypeptide in the said protein sequence is preferably coronavirus SARS-CoV related S, M, E and N protein polypeptide sequence. The present invention also relates to the amino acid sequence with SARS-CoV antigen determinant function, such as those shown in SEQ ID No. 1 to SEQ ID No. 112. The present invention also relates to one kind of 'mixing-separating' physical encoding method for synthesizing peptide library with at least 2-50 amino acid residues.

The invention discloses a method for controlling a universal-energy network by a universal-energy current sequence parameter, which comprises the following steps of: analyzing the universal-energy current relationship of production, storage, application and regeneration in a universal-energy network system; according to a natural sequence parameter rule, determining the key sequence parameter of each level from the universal-energy current relationship, wherein the key sequence parameter is used for quickly evaluating and optimizing integral system performance; according to a level sequence parameter rule, determining the performance optimization order of the universal-energy network system; according to the optimization order, successively optimizing the key sequence parameter of each level; and according to the optimal link proportion rule, determining the optimal proportion of four links, wherein the optimal proportion of four links is used for lowering the redundant universal-energy current of the system. With the method, the performance of the universal-energy network system can be comprehensively optimized.

The invention discloses a method for separating a natural sequence nerve growth factor from a mixture. A solution containing a human or mouse nerve growth factor is separated and purified through metal ion chelating chromatography to obtain the natural sequence nerve growth factor; the metal ion chelating chromatography resin comprises a supporting medium, a chelating ligand and metal ions; the metal ions are chelated on the chelating ligand; the elution condition is to change the concentration gradient of protein and metal ion coordinate bound competitive materials. Strong cation exchange chromatography can be conducted before metal ion chelating chromatography; after metal ion chelating chromatography, efficient strong cation exchange chromatography can be conducted. According to the method, the nerve growth factor with natural sequence and without any protein tags can be purified and separated rapidly.

The present invention concerns a new designer cytokine termed H11, which is constructed by fusion of two soluble components, soluble interleukin 11 receptor (sIL-11 R) and interleukin 11 (IL-11) using their natural sequence and its use for the production of a medicament for treating or preventing a disease selected from the group consisting of a proliferative disease, a cytopathy, radiation damage, an IL-11 dependent inflammatory disorder, IL-11 dependent degenerative disorder and IL-11 dependent or mediated soft tissue disorder.

The invention relates to an infectious cDNA of foot-and-mouth disease virus of type Asia 1 and the construction method, wherein, the RNA transcribed from the infectious cDNA contains the host cell of the infectious cDNA. The construction method is as follow: the foot-and-mouth disease virus of type Asia 1 is used as template; the genome segments are expanded by reverse transcription using specific primer; the expanded segments are assembled and cloned to a carrier; T7RNA polymerized polymerase promoeter is positioned at the upper reach of the obtained 5' end of cDNA; the 3' end is provided with single restriction enzyme cutting site and tail end Poly (A) sequence; the difference between the infectious cDNA and virus genome natural sequence is less than thirty nucleotides. The infectious cDNA of foot-and-mouth disease virus of type Asia 1 can be used to research the pathogenesis of virus and the replication and the expression and regulation mechanism of genome, and also can be used to prepare marked attenuated strain vaccine and develop new foot-and-mouth disease vaccine with gene manipulation technique.

The invention discloses a host computer invasion detection method based on the natural subsequence mode decomposition. The method includes the following steps: firstly, defining rules; obtaining Windows Native API data sequence, decomposing process sequences into natural subsequence mode sets and then layering the natural subsequence modes according to the support degree; thirdly, decomposing suspected sequences into a plurality of layers respectively containing natural sequence modes with similar support degrees; fourthly, matching the normal process sequences with the suspected sequences according to the corresponding layers, calculating the abnormal degree according to the matched number and judging if the suspected sequences are abnormal. The method overcomes the disadvantages existed in the prior art and can accurately and effectively identify the current attacks and the new increasing attacks.

The invention provides a production method for recombinant human fibroblast growth factor-17 protein. The production method at least comprises the following steps: step 1, performing recombination and amplification on human fibroblast growth factor-17 protein FGF-17 with a natural sequence in accordance with preference of colibacillus; step 2, connecting the recombinant rhFGF-17 gene to a colibacillus expression plasmid to form a recombinant expression vector, and transferring the recombinant expression vector to enable the recombinant expression vector to enter colibacillus host bacteria to obtain FGF-17 protein expression engineering bacteria; step 3, cultivating the expression engineering bacteria and then purifying, wherein in the purifying process, a cell lysis buffer solution comprises the following concentrations of components: 45-55 mM TrisTris, 280-320 mM sodium chloride (NaCl), 1.5-2.5 mM ethylenediamine tetraacetic acid (EDTA), 0.1-0.3 M cane sugar, 0.8-1.2% of polyethylene glycol octylphenyl ether TritonX-100 in percentage by volume, 0.1-0.3% of sodium deoxycholate in percentage by volume, 5-10% of glycerol in percentage by volume, and 0.8-1.2% of phenyl methyl sulfonyl fluoride (PMSF) in percentage by volume. In the production method provided by the invention, the concentration and the purity of the FGF-17 protein are high.

The invention provides an inter-node bus accessing priority deploying method based on multiple servers. Firstly, according to the server node bus communication requirements, grouping integration is carried out on key physical signals participating in bus communication logic through a programmable logic controller; meanwhile, transmission starting monitoring on high speed data of all parallel nodes is carried out; the actual value cooperation requirements such as time sequences and triggering priority requirements of the bus signals are achieved in a DSP in a built-in mode. The principle is that in the bus idle stage, buses are sequentially switched and connected among the nodes according to a natural sequence in order to guarantee the data response instantaneity; in the bus busy stage, besides consideration to the natural sequence, judgment on the waiting time is further added, and the nodes with the long waiting time preferentially have access in bus communication. Thus, the time sequences and the triggering requirements of the bus signals are achieved through DSP software languages, bus communication signals collected by the programmable logic controller are transmitted to a DSP data processing center, a DSP judges the actual values such as the time sequences and the triggering requirements of each participant signal according to the preset timing sequence requirements, and whether the bus switching requirements are met or not is further judged, and resource balanced configuration is achieved.

The invention discloses a fused type prokaryotic expression vector, and a construction method and application thereof. The fused type prokaryotic expression vector comprises a promoter, a purification tag gene, a protease cleavage site, a multiple cloning site and a terminator, wherein a Beta 2 microglobulin gene is connected between the purification tag gene and the protease cleavage site. According to the fused type prokaryotic expression vector, the Beta 2 microglobulin (Beta 2M) gene is contained in the fused type prokaryotic expression vector, a foreign protein which is hard to express is connected to the downstream of the Beta 2M gene, thus the excellent characteristics of the Beta 2M gene expression vector can be fully utilized while the expression carrier expresses the foreign protein which is hard to express, the foreign protein can be effectively expressed in a host cell, and the target protein can be conveniently renatured and separated and purified in the later period; in addition, a purification tag and an enzyme cleavage site are contained in the fused type prokaryotic expression vector, thus the protein can be conveniently separated and purified after the expression, and the purification tag and the Beta 2M protein also can be conveniently removed, and as a result, the target protein closer to the natural sequence can be gained.

In allusion to the problem that a modulator of a routine Turbo code stream has no encryption function, a modulation and encryption method based on key control is designed according to a random modulation-based combined channel and secure coding and decoding design method. By the method, an interleaver is associated with a modulator. Firstly, length of the interleaver is set as N; S is natural sequence of the length N; a Key is adopted to generate a random permutation d of natural numbers having the length N, wherein d=f(S,Key), and f is a random permutation function; mod-2 operation is carried out on d to generate a binary sequence b having the length N; and finally, random modulation is carried out on a reused coded bit sequence c, and a modulation sequence cm is outputted. During demodulation, a receiving end adopts the same Key and random function f with a coding end to generate the binary sequence b having the length N, and demodulation is carried out to output the correct bit sequence c.

The invention provides high-speed parallel Turbo decoding method and system in a software radio system. The method comprises the following steps: dividing data to be decoded into M segments, and reordering the data to continuously store the corresponding data at the same positions in the M segments; carrying out iterative decoding on the reordered data to be decoded, comprising the processes of performing parallel component code decoding on the data in the M segments and performing interlacing and de-interlacing on the exchange external information of the two component codes through an interlacing list calculated in advance; and calculating the log likelihood of a system bit, performing symbol judgment, and reordering the judged decoding bit sequences to recover the natural sequence. According to the embodiment of the invention, the high-speed parallel Turbo decoding in the software radio system is realized, the decoding time is greatly reduced and the decoding throughout rate is improved.

A strategy game providing an educational and entertaining activity for children, adults, illiterate people, dyslexic people and players who do not speak a common language. The game comprises a game board, tiles, and cards. The game board includes a plurality of branching runways extending from a common starting space and ending in a plurality of ending spaces. Each branching runway is divided into a plurality of spaces. A subset of the spaces are special spaces and are identified as special spaces by at least one of design and indicia. The tiles comprise a plurality of sets of unique tiles, each tile of each set having at least one of a design, indicia, and color identifying a place in a natural sequence of tiles of the set, such that each set contains a complete natural sequence. The cards each have one of a number of unique designs. The plurality of tiles may be laid in the natural sequence on the spaces, and the cards are drawn by players to form a complete set based on how the tiles are laid on the game board.

The invention provides optimized hIL-2 (human interleukin-2) genes as well as an expression method and an application thereof. Human-derived IL-2 is transformed with a two-step optimization method including codon optimization and amino acid sequence optimization. The expression quantity of hIL-2 obtained after codon optimization is improved by 132.1% than that of a natural sequence in pichia pastoris, and the expression quantity after amino acid sequence optimization is further improved by 96.5%. The invention also provides an industrial high-density fermentation method of the hIL-2. The invention also provides the application of the hIL-2 in breeding of white feather broilers and weaned piglets. The hIL-2 improves the growth condition of the white feather broilers, reduces inflammatory factors in the white feather broilers and greatly improves immunity of the white feather broilers after being added to feed; the hIL-2 improves the feed intake and daily average weight gain of the weaned piglets and reduces diarrhea rate of the weaned piglets after being added to weaned piglet ration.

The invention discloses a data encryption transmission method, relates to the technical field of data encryption, and solves the technical problem of low data encryption and transmission security caused by adopting a fixed key to encrypt and decrypt data in the prior art. The plaintext data is divided into a plurality of sub-data according to the set unit quantity, the plurality of sub-data are numbered, the number and the corresponding sub-data are reordered according to the natural sequence, the number sequence and the data sequence are obtained, the number sequence and the data sequence are encrypted through different secret keys, and the purpose of data encryption is achieved. The safety of the data is improved through double encryption; according to the method, before the numbers and the corresponding sub-data are reordered, the position feature tags corresponding to the numbers are obtained, the reference nodes are randomly selected, and the reference nodes can be quickly determined according to the number sequence; according to the invention, the data cracking difficulty is improved, and the data security is further ensured.

The invention provides an L-lysine fermentation method. The method comprises the following steps: introducing polynucleotides which code pyridine nucleotide transhydrogenase in a non-natural sequence into bacteria which can generate L-lysine to make the obtained bacteria to express the pyridine nucleotide transhydrogenase; and cultivating the obtained bacteria under a fermentation condition. The invention also provides intermediate products used in the fermentation method, and further product production with the fermentation, and the like.

The invention relates to a parallel RP interleaving method for Turbo codes, and a parallel RP interleaver for the Turbo codes. The interleaver comprises a matrixing module, a line permutation module, a row permutation module, and a screening module, wherein the matrixing module writes a natural sequence in an original order into a matrix according to the order; the line permutation module adopts the line RP criterion for each line in the matrix; the row permutation module adopts the row RP criterion for each row in the matrix; and the screening module carries out screening on parameter values in the line RP criterion and the row RP criterion, and selects the interleaver with the largest d<free> as a final interleaver. The interleaver provided by the invention has the advantages of good decoding performance, low complexity, and small hardware storage volumes; and the decoding performance of the interleaver can exceed that of a standard QPP interleaver.

Episomally transfected primary mesenchymal stem cells (MSC) express a polypeptide consisting of an antigenic polypeptide (e.g., one or more polypeptides) relating to a pathogen (e.g., one or more virus, bacterium, or parasite). The antigenic polypeptide can have the amino acid sequence of a natural polypeptide from the pathogen or an amino acid sequence differing from the natural sequence by one or more conservative amino acid substitutions. Uses and method for treating or preventing infections with episomally transfected primary MSC also are described.

The invention discloses a fused type prokaryotic expression vector, and a construction method and application thereof. The fused type prokaryotic expression vector comprises a promoter, a purification tag gene, a protease cleavage site, a multiple cloning site and a terminator, wherein a Beta 2 microglobulin gene is connected between the purification tag gene and the protease cleavage site. According to the fused type prokaryotic expression vector, the Beta 2 microglobulin (Beta 2M) gene is contained in the fused type prokaryotic expression vector, a foreign protein which is hard to express is connected to the downstream of the Beta 2M gene, thus the excellent characteristics of the Beta 2M gene expression vector can be fully utilized while the expression carrier expresses the foreign protein which is hard to express, the foreign protein can be effectively expressed in a host cell, and the target protein can be conveniently renatured and separated and purified in the later period; in addition, a purification tag and an enzyme cleavage site are contained in the fused type prokaryotic expression vector, thus the protein can be conveniently separated and purified after the expression, and the purification tag and the Beta 2M protein also can be conveniently removed, and as a result, the target protein closer to the natural sequence can be gained.

The invention provides a priority allocation method based on bus access among multiple server nodes. Firstly, according to the bus communication requirements of the server nodes, the key physical signals participating in the bus communication logic are grouped and integrated using a programmable logic controller; at the same time, each node is parallelized The initial monitoring of high-speed data transmission; the time sequence of the bus signal, the trigger priority requirements and other actual values ​​are matched with the requirements in the DSP. The principle is that the bus is idle. Nodes are switched and connected sequentially; when the bus is busy, in addition to considering the natural ordering, the judgment of the waiting time is also added, and the nodes with a longer waiting time are given priority to access the bus communication; the time sequence and trigger requirements of each bus signal are adopted. DSP software language implementation; the programmable logic controller transmits the collected bus communication signals to the DSP data processing center, and the DSP judges the actual values ​​such as the time sequence and trigger requirements of the participating signals according to the pre-scheduled requirements, and then gives the judgment Whether it meets the requirements of bus switching and achieves a balanced allocation of resources.

The present invention provides a method for producing recombinant human fibroblast growth factor-17 protein, at least including the following steps: step 1, recombining natural sequence human fibroblast growth factor-17 protein FGF-17 according to the preference of Escherichia coli and amplification; step 2, connecting the recombined rhFGF‑17 gene to the E. coli expression plasmid to form a recombinant expression vector, and then transforming the recombinant expression vector into the E. coli host bacterium to obtain FGF‑17 protein expression engineering bacteria; and steps 3. Cultivate the expression engineering bacteria, and then purify; wherein, in the purification process, the cell lysis buffer of the following concentration components is used: 45-55mM tris Tris, 280-320mM sodium chloride NaCl, 1.5-2.5mM ethylenediaminetetraacetic acid DTA, 0.1-0.3M sucrose, and 0.8-1.2% volume percentage of polyethylene glycol octyl phenyl ether TritonX-100, 0.1-0.3% Sodium oxycholate, 5%-10% glycerin, 0.8-1.2% phenylmethylsulfonyl fluoride PMSF. The concentration and purity of the FGF‑17 protein produced by the production method are high.