The invention relates to a primer for detecting main drug-resistant mutation sites of an aids therapeutic nucleoside reverse transcriptase inhibitor. The primer comprises ASPCR primers for detecting the mutation sites M41L, D67N, K65R, K70R, K70E, M184V, M184I, L210W, T215Y and T215F of HIV-1 virus pol genes. Each mutation site comprises a pair of wild PCR amplification primers and a pair of mutation PCR amplification primers. Specific upstream primers of each mutation site primer pair are shown in SEQ ID NO.1 to SEQ ID NO.20, and a universal downstream primer is shown in SEQ ID NO.21. The primer is used for detecting the drug-resistant mutation sites of the aids therapeutic nucleoside reverse transcriptase inhibitor and has the advantages of being high in specificity, high in flexibility, easy, convenient and rapid to use, low in cost and the like.