Process for preparing retrovirus vector for gene therapy

a technology of retrovirus and gene therapy, applied in the field of process for preparing retrovirus vector for gene therapy, can solve the problems of insufficient therapeutic effect, no technique has been established, and it is difficult to prepare vectors with such high qualities, and achieve the effect of high titer and high screening efficiency of pre-packaging cells
US20010018203A1Inactive Publication Date: 2001-08-30EISIA R&D MANAGEMENT CO LTD
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Patent Information

Authority / Receiving Office
US · United States
Patent Type
Applications(United States)
Current Assignee / Owner
EISIA R&D MANAGEMENT CO LTD
Publication Date
2001-08-30
Estimated Expiration
Not applicable · inactive patent

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Abstract

The present invention provides a process for preparing a retrovirus to be expressed at a high titer by specifically transferring a desired foreign gene into target cells. A pseudotyped retrovirus vector having a high titer can be prepared by transferring a DNA construction wherein a promoter, an loxP sequence, a VSV-G gene and a polyA addition signal are arranged in this order is transferred into cells carrying the retrovirus gag and pol gene expression systems, and then transferring a retrovirus vector containing the desired foreign gene thereinto, followed by the treatment with a recombinase.
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Description

[0001] 1. Technical Field

[0002] The present invention relates to a process for preparing a retrovirus vector having a high titer and employed in gene therapy.

[0003] 2. Prior Art

[0004] Owing to the remarkable progress in genetic engineering in recent years, there have been identified genes causative of a number of genetic diseases and thus the pathological mechanisms of these diseases have been clarified at the molecular level. Under these circumstances, studies on gene therapy have been made to transfer genes seemingly capable of ameliorating diseases into cells and some of these treatments have been already put into practical use. Also, attempts have been made to apply the gene therapy to the treatment of cancer, AIDS, etc. In the gene therapy, there are known several methods for transferring foreign genes. Among all, the most frequently employed method at the present stage is the one with the use of retrovirus vectors (Miller, A. D., Blood, 76, 271-278, 1990). Use of these vectors...

Claims

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