The invention provides a method for large-scale suspension culture of fowl adenoviruses. The method provided by the invention can be used for obtaining high-titer fowl adenoviruses of I group 4 type, 8b type, D11 type, 8a type and the like, a domesticated LMH cell used in the method is a chicken liver cancer suspension LMH cell, and the collection number of the domesticated LMH cell is CCTCC NO: C2021202. According to the method disclosed by the invention, adherent LMH cells are domesticated into suspension cells capable of realizing stable and continuous passage by adopting a gradual serum reduction method. Then, full suspension culture is carried out on the LMH cells by using a bioreactor, then, step-by-step amplification suspension culture is carried out on the LMH cells, and then, aviadenovirus antigens are respectively inoculated and proliferated on the suspension cells. By use of the method disclosed by the invention, high-titer aviadenovirus suspension culture technology virus antigens can be obtained, and the titer of the antigens is about 5-10 times of that of existing vaccine regulation antigens.