A recombinant fowlpox virus transfer vector expressing a fowl adenovirus serotype-4 fiber2 gene, a constructing method thereof and applications of the transfer vector

A technology of fowlpox virus and transfer vector, which is applied in the direction of virus/bacteriophage, application, virus, etc. It can solve the problems of poor immune effect, slow antibody production, and unsatisfactory effect, and achieve the effect of high cost and improved efficiency
CN107475296AActive Publication Date: 2017-12-15WENS FOODSTUFF GRP CO LTD
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
WENS FOODSTUFF GRP CO LTD
Publication Date
2017-12-15

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Abstract

The invention belongs to the technical field of biology, and mainly relates to a recombinant fowlpox virus transfer vector expressing a fowl adenovirus serotype-4 (FADV4) fiber2 gene, a constructing method thereof and applications of the transfer vector. A pMD19T-Simple vector is adopted as a base of the transfer vector. The FADV4 fiber2 gene, a lacz gene, and fowlpox virus genome replicated non-essential fragments which are LTYB and RTYB used for homologous recombination are inserted in a TA cloning site. The method includes constructing a plasmid pMD-TYB; constructing a plasmid pMD22; constructing a plasmid pMD22-lacz; constructing an intermediate vector pMD22-TYB-lacz; amplifying the FADV4 fiber2 gene; constructing a recombinant fowlpox virus transfer vector pMD22-TYB-lacz-F4; subjecting a chicken embryo Fibroblast to cotransfection with the transfer vector pMD22-TYB-lacz-F4 and a fowlpox virus; performing identification to select a positive product; performing subculture continuously; and identifying expression effects of the recombinant fowlpox virus. The recombinant fowlpox virus transfer vector constructed by the method lays a foundation for development of an efficient recombinant fowlpox virus genetic engineering living-vector vaccine expressing the fowl adenovirus serotype-4.
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Description

technical field

[0001] The invention belongs to the field of biotechnology, and mainly relates to a recombinant fowlpox virus transfer vector expressing the fiber2 gene of chicken type 4 adenovirus (FADV4) and its construction method and application. Background technique

[0002] Fowl adenovirus (Fowl adenovirus, FAdV) is a double-stranded DNA virus without an envelope. The avian adenoviruses with different antigens can be divided into 3 groups: Ⅰ, Ⅱ and Ⅲ. Group I avian adenoviruses are mainly obtained from chickens, turkeys, geese and other poultry, and are divided into five species (A-E). The viruses in each species are further divided into 12 serotypes based on the results of cross-neutralization tests ( FAdV1-12). Avian adenovirus C includes avian adenovirus types 4 and 10 (FAdV-4, FAdV-10), and its main clinical and pathological syndromes are hydropericardium syndrome and inclusion body hepatitis.

[0003] The capsid of avian adenovirus is mainly composed of hexon a...

Claims

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