Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

F2 protein based indirect ELISA kit for detection of fowl adenovirus serotype 4 antibody

A poultry adenovirus and kit technology, applied in the field of biotechnology detection, to achieve good specificity

Inactive Publication Date: 2018-01-19
YANGZHOU UNIV
View PDF4 Cites 9 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no rapid method and kit for FAdV-4 serological antibody detection at present

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • F2 protein based indirect ELISA kit for detection of fowl adenovirus serotype 4 antibody
  • F2 protein based indirect ELISA kit for detection of fowl adenovirus serotype 4 antibody
  • F2 protein based indirect ELISA kit for detection of fowl adenovirus serotype 4 antibody

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] The indirect ELISA kit for detecting serum type 4 avian adenovirus antibody based on the F2 protein of the present invention can be obtained by the following steps:

[0025] (1) Isolation of FAdV-4 avian adenovirus:

[0026] Take the liver of a dead chicken suspected of avian adenovirus infection, grind it and add PBST at a ratio of 1:5 to make a suspension; centrifuge at 5000r / min for 15min, and take the supernatant; React at 37°C for 30 minutes; filter through a 0.45um microporous filter, inoculate 8-day-old SPF chicken embryos through the allantoic cavity at a dose of 0.2ml / embryo, collect allantoic fluid 96-120 hours after inoculation; After being identified as FAdV-4 avian adenovirus, it was stored at -20°C.

[0027] (2) Preparation of avian adenovirus genome:

[0028] Take 400uL supernatant of FAdV-4 isolated strain virus (serotype 4 avian adenovirus) in a 1.5mL finger tube, add 400uL cell lysate (50mmol / L Tris-HCl pH 8.0, 20mmol / L EDTA pH 8.0, 2% SDS and prote...

Embodiment 2

[0043] Clinical sample detection effect

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses an F2 protein based indirect ELISA kit for detection of fowl adenovirus serotype 4 (FAdV-4) antibody. The kit includes an ELISA plate coated with a spike protein gene expression product, a positive and negative control, an HRP labeled rabbit anti-chicken secondary antibody, a sample diluent, a color development liquid and a washing liquid. The indirect ELISA kit for FAdV-4antibody established by the invention can specifically detect the FAdV-4 antibody, and does not react with anti-influenza virus (AIV) serum, anti-mardivirus (MDV) serum, anti-Newcastle disease virus (NDV) serum, anti-avian reticuloendotheliosis virus (REV) serum and SPF chicken serum (Figure 6). Therefore, the kit has good FAdV-4 specificity, and can be used for FAdV-4 infection and immune statusepidemiological investigation.

Description

technical field [0001] The invention belongs to the field of biotechnology detection, and in particular relates to an indirect ELISA kit for detecting serum type 4 avian adenovirus antibody based on F2 protein. Background technique [0002] Fowl Adenovirus (Fowl Adenovirus, FAdV) belongs to Adenoviridae, and is divided into 5 species (A-E) and 12 serotypes. Although it has been reported all over the world, FAdV infection generally causes subclinical conditions, while acute infection mainly causes inclusion body hepatitis, pericardial effusion, and gizzard erosion. Since 2013, cases of inclusion body hepatitis and pericardial effusion caused by FAdV in domestic chicken flocks have gradually increased. By 2015, FAdV infection broke out in chicken flocks in several provinces in China. At present, the outbreak of FAdV in China not only occurs in 3-4 week-old broilers, but also occurs in 10-20 week-old laying hens, causing serious economic losses to the chicken industry. Virus...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): G01N33/569G01N33/543G01N33/535C07K14/075C12N15/70
Inventor 叶建强王伟康邵红霞秦爱建田晓彦梁广成万志敏
Owner YANGZHOU UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com