Fowl adenovirus serum type 4 recombinant virus for expressing fowl adenovirus serum type 8b spike protein as well as construction method and application of fowl adenovirus serum type 4 recombinant virus

A technology of avian adenovirus and spike protein is applied in the field of avian adenovirus serotype 4 recombinant virus and its construction, and can solve the problem of high cost of vaccine production

Pending Publication Date: 2022-03-22
HENAN AGRICULTURAL UNIVERSITY
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Problems solved by technology

The preparation of traditional multiple vaccines requires a series of cumbersome processes such as separately cultivating various pathogens, then concentrating a

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  • Fowl adenovirus serum type 4 recombinant virus for expressing fowl adenovirus serum type 8b spike protein as well as construction method and application of fowl adenovirus serum type 4 recombinant virus
  • Fowl adenovirus serum type 4 recombinant virus for expressing fowl adenovirus serum type 8b spike protein as well as construction method and application of fowl adenovirus serum type 4 recombinant virus
  • Fowl adenovirus serum type 4 recombinant virus for expressing fowl adenovirus serum type 8b spike protein as well as construction method and application of fowl adenovirus serum type 4 recombinant virus

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[0063] In the following, the present invention will be described in more detail through specific embodiments, so as to facilitate the understanding of the technical solution of the present invention, but it is not used to limit the protection scope of the present invention.

[0064] The construction method of the FAdV-4 recombinant virus expressing FAdV-8b Fiber may further comprise the steps:

[0065] 1. Construction of the genome infectious clone p15A-cm-HNJZ of the FAdV-4 epidemic strain CH / HNJZ / 2015 in China

[0066] 1.1. Construction of p15A-cm backbone vector containing FAdV-4 homology arms

[0067] Using the p15A-cm-tetR-tetO-ccdB-hyg plasmid as a template, the upstream and downstream primers containing the inverted terminal repeats at the left and right ends of the FAdV-4 genome were used to amplify the genome of the FAdV-4 Chinese epidemic strain CH / HNJZ / 2015 The backbone carrier of the inverted terminal repeat sequence at the left and right ends, the amplified backb...

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Abstract

The invention belongs to the field of animal genetic engineering vaccines, and particularly relates to a fowl adenovirus serum type 4 (FAdV-4) recombinant virus for expressing fowl adenovirus serum type 8b (FAdV-8b) spike protein (Fiber) as well as a construction method and application of the fowl adenovirus serum type 4 (FAdV-4) recombinant virus. The recombinant virus is obtained by replacing a FAdV-4 Fiber1 gene with a Fiber gene of FAdV-8b, the recombinant virus can be used for preparing a bivalent vaccine for preventing and controlling chicken hepatitis-hydropericardium syndrome and/or chicken inclusion body hepatitis, and the bivalent vaccine prepared by utilizing the recombinant virus can achieve the effect of preventing two epidemic diseases at the same time by injecting a needle of vaccine. Therefore, the purpose of preventing and controlling two or more diseases by one needle can be achieved by inserting the FAdV-4 serving as a carrier into an exogenous gene.

Description

technical field [0001] The invention belongs to the field of animal genetic engineering vaccines, and in particular relates to a recombinant virus of poultry adenovirus serotype 4 expressing the spike protein of poultry adenovirus serotype 8b and its construction method and application. Background technique [0002] my country is a large breeding country in the world, and the breeding industry occupies an important position in the national economy. Livestock and poultry diseases are one of the important bottlenecks that seriously hinder the sustainable development of the breeding industry. Fowl aviadenovirus (Fowl aviadenovirus, FAdV) belongs to Adenoviridae, Avian Adenovirus genus, is a non-enveloped double-stranded DNA virus. The avian adenovirus genus currently includes 12 serotypes in 5 species, namely A (FAdV-1), B (FAdV-5), C (FAdV-4, -10), D (FAdV-2, -3, - 9, -11) and E (FAdV-6, -7, -8a, -8b). Avian adenoviruses are widely present in farms, and the clinical diseases...

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Application Information

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IPC IPC(8): C12N7/01C12N15/64C12N15/65C12N15/861C12N15/34A61K39/235A61P1/16A61P31/20C12R1/93
CPCC12N7/00C12N15/64C12N15/65C12N15/86C07K14/005A61K39/12A61P31/20A61P1/16C12N2710/10221C12N2710/10234C12N2710/10222C12N2710/10243A61K2039/5252A61K2039/552A61K2039/70
Inventor 赵军王白玉宋明珍乔麒龙黄庆杨盼盼
Owner HENAN AGRICULTURAL UNIVERSITY
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