10 results about "Adenovirus typing" patented technology

The invention provides an anti-duck adenovirus 3 type monoclonal antibody, a hybridoma cell strain and application thereof, the anti-duck adenovirus 3 type monoclonal antibody is secreted by a hybridoma cell strain 2F12, the classification name of the hybridoma cell strain 2F12 is Hybridoma cell line 2F12, the hybridoma cell strain 2F12 is preserved in China Center for Type Culture Collection on November 13, 2025, the preservation address is Wuhan University, Wuhan, China, and the preservation number is CGMCC NO. The preservation number of the strain is CCTCC (China Center For Type Culture Collection) NO: The blocking ELISA kit for detecting the duck adenovirus 3 antibody, which is prepared by utilizing the monoclonal antibody, is strong in specificity, high in sensitivity and good in repeatability, and has a very good application prospect in the aspects of epidemiological investigation of the duck adenovirus 3, vaccine immune efficacy evaluation and diagnosis of the duck adenovirus 3 disease.

This invention relates to the quantitative detection of pigeon adenovirus and the simultaneous differential diagnosis of type I and type II. Specific primers and probes for pigeon adenovirus type I and II are designed using specific gene sequences. Real-time quantitative PCR can accurately detect and differentiate between pigeon adenovirus types I and II. Verification has shown that this technology can rapidly and accurately differentiate between PiAdV-I and PiAdV-II, exhibiting high specificity and sensitivity. Furthermore, it is cost-effective, easy to operate, and suitable for import / export quarantine and specialized laboratories for the detection and differentiation of PiAdV-I and PiAdV-II.

The application discloses a method for adenovirus whole genome sequencing, a primer group and a kit thereof, and belongs to the technical field of DNA detection.The method for adenovirus whole genome sequencing comprises the following steps: S1, first round amplification is carried out by using first round amplification primers with sequences shown in SEQ ID NO.1 to SEQ ID NO.93 in Table 1; and S2, second round amplification is carried out by using second round amplification primers with sequences shown in SEQ ID NO.94 to SEQ ID NO.285 in Table 2.The average length of data sequenced by the method is 0.8 kb, compared with short read length of a second-generation platform, the uniformity of adenovirus coverage can be greatly improved, meanwhile, the adenovirus types are comprehensively covered, and the method can be widely promoted.

This invention belongs to the technical field of virus detection, specifically relating to a PCR detection kit for the simultaneous detection of four DNA viruses in waterfowl and its application. The PCR detection primer set for the simultaneous detection of four DNA viruses in waterfowl of this invention is the optimal primer set obtained through experimental screening. The kit composed of these primers can rapidly and specifically amplify and diagnose Muscovy duck parvovirus, duck plague virus, duck adenovirus type 3, and duck circovirus simultaneously, exhibiting high specificity and sensitivity. It can accurately and rapidly identify MDPV, DPV, DAdV-3, and DuCV, and can complete quantitative real-time PCR detection in approximately 50 minutes. The kit is simple and practical to operate, achieving efficient simultaneous detection of four viruses, shortening the detection cycle, increasing sensitivity by 10-1000 times, significantly improving early disease diagnosis capabilities, significantly reducing the misdiagnosis rate of diseases in poultry farms, and contributing to the sustainable development of the poultry farming industry.

The present disclosure relates to primer and probe sets for pathogen detection of infection in a transplant patient, a kit and use thereof, and belongs to the technical field of molecular biology detection. There are 23 primer and probe sets that can be used to jointly detect 23 kinds of pathogens with a high infection rate and a high lethality rate after transplantation, including an adenovirus type B; and two ends of a corresponding sequence of each probe have correspondingly a fluorophore and a quencher group, respectively. The present disclosure further provides a real-time fluorescence quantitative PCR kit for pathogen detection of infection in a transplant patient, including the primer and probe sets, a pathogen plasmid standard, a fluorescence quantitative PCR reaction solution, and sterile deionized water, which can simultaneously detect 23 pathogens infected by the transplant patient.

The invention belongs to the technical field of veterinary biotechnology detection, and particularly relates to a monoclonal antibody for resisting duck adenovirus 3 type Fiber2 protein and application of the monoclonal antibody to detection or identification of duck adenovirus 3 type. The duck adenovirus 3 type Fiber2 protein is taken as an immunogen to screen a hybridoma cell strain with good reactivity to different strain whole viruses of the duck adenovirus 3 type, an antibody generated by the hybridoma cell strain is analyzed, the monoclonal antibody resisting the duck adenovirus 3 type Fiber2 protein is obtained, and the sequence information is shown in a table 3 in the specification. The monoclonal antibody provided by the invention can identify different strains of the duck adenovirus 3, does not generate cross reaction with other common poultry disease viruses such as fowl adenovirus, novel duck reovirus and the like, has good specificity and sensitivity, can be used for detecting the exogenous virus of the duck adenovirus 3 in poultry virus live vaccines, and has good application prospects. And the kit can also be used for clinical identification, virus content determination and epidemiological investigation of the duck adenovirus type 3.

To provide methods for treating human patients with replicable oncolytic adenovirus.SOLUTION: Disclosed is a method comprising the steps of: systemically administering multiple doses of a parenteral formulation of a replicable oncolytic adenovirus of subgroup B in a single treatment cycle, where the total dose given in each dose is in the range of 1×1010-1×1014 viral particles, and where each dose of virus is administered over a period of 1 to 90 minutes, for example, at a rate of viral particle delivery in the range of 2×1010 particles per minute to 2×1012 particles per minute. The disclosure further extends to formulations of the oncolytic adenoviruses and combination therapies of the viruses and formulations with other therapeutic agents.SELECTED DRAWING: None

The invention relates to the technical field of biological detection, in particular to a primer and probe combination for detecting and identifying tortoise adenovirus type 1-3, and the primer and probe combination comprises an upstream primer, a downstream primer and a probe for detecting or / and identifying the tortoise adenovirus type 1-3; the nucleotide sequences of the upstream primers of the tortoise serrated adenovirus type 1-3 are respectively as shown in SEQ ID NO: 1-3, the nucleotide sequences of the downstream primers of the tortoise serrated adenovirus type 1-3 are respectively as shown in SEQ ID NO: 4-6, and the nucleotide sequences of the probes of the tortoise serrated adenovirus type 1-3 are respectively as shown in SEQ ID NO: 7-9. The primer probe combination disclosed by the invention can be used for real-time fluorescent quantitative PCR and multiple PCR technologies, the type-1-3 of the serrate turtle adenovirus can be simultaneously detected in a one-tube PCR reaction system, and three typing detection results of the serrate turtle adenovirus can be obtained by only carrying out one-time nucleic acid extraction and one-time PCR detection on one sample. The method is simple, accurate in result and easy to popularize. Meanwhile, the primer and the probe disclosed by the invention are high in sensitivity and good in specificity.

The invention discloses a recombinant serum type 3 duck adenovirus and a construction method and application thereof, the recombinant serum type 3 duck adenovirus replaces a non-structural protein gene ORF52 in the serum type 3 duck adenovirus with an exogenous target gene or inserts the exogenous target gene into the non-structural protein gene ORF52, and the nucleotide sequence of the non-structural protein gene ORF52 is shown as SEQ ID NO. 1. The recombinant serum type 3 duck adenovirus for expressing green fluorescent protein is constructed by targeting an exogenous gene EGFP to a non-structural protein gene ORF52 of the duck adenovirus type 3 by utilizing a gene editing technology, and the non-structural protein gene ORF52 is proved to be capable of serving as an insertion site of the exogenous gene, so that the recombinant serum type 3 duck adenovirus can be used as an insertion site of the exogenous gene. And a foundation is laid for developing a genetic engineering recombinant DAdV-3 multi-combined multivalent vaccine based on taking DAdV-3 as a virus vector.

The invention provides an adenovirus vector as well as a construction method and application thereof, and the recombinant adenovirus vector is named as pAd5-C7. According to the invention, a Tet gene regulation and control system and a Cumate gene regulation and control system are integrated into a single-copy plasmid pAd5 containing an adenovirus type 5 genome in different ways to obtain a recombinant adenovirus vector pAd5-C7. On the basis of a Rembineering technology, a screening gene SacB / beta-geo is inserted into the downstream of a CMV promoter on pAd5-C7, and a rapid, efficient and universal recombinant adenovirus vector construction platform is constructed. Recombinant adenovirus plasmids constructed on the basis of the platform can be used for obtaining recombinant adenovirus particles with advanced protein regulated and controlled. In an adenovirus vector based on the human adenovirus type 5, the recombinant adenovirus which is normally expressed in allowable cells and is inhibited in advanced protein expression of the adenovirus in non-allowable cells is prepared.