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Probe and kit for detecting human adenovirus 7 and use method of kit

A kit and adenovirus technology, applied in the field of human adenovirus type 7 detection, can solve the problems of false positives, time-consuming and high cost, and achieve the effects of simple operation, lowering reaction temperature and saving reaction time

Pending Publication Date: 2021-09-07
国药(武汉)医学实验室有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Traditional detection methods include virus isolation and identification, immunofluorescence and common PCR detection methods, which have problems such as high cost, time-consuming, and false positives, and cannot provide etiological evidence for epidemic prevention departments in a timely manner

Method used

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  • Probe and kit for detecting human adenovirus 7 and use method of kit
  • Probe and kit for detecting human adenovirus 7 and use method of kit
  • Probe and kit for detecting human adenovirus 7 and use method of kit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0045] A kit for detecting human adenovirus type 7 nucleic acid based on MIRA fluorescence method, the kit is equipped with: Abuffer, B buffer, first primer, second primer, fluorescent probe, ddH 2 O, negative quality control, positive quality control.

[0046] Wherein, referring to the instructions of the magnetic bead method nucleic acid extraction kit, the isolated culture of human adenovirus type 7 was used as a positive quality control.

[0047] Wherein, the finished kit includes the following:

[0048] Reagent name content Abuffer 1.6mL×1 tube B buffer 150uL×1 tube positive control template 100uL×1 tube Positive Control Primer Probe Mix 200uL×1 tube negative control template 100uL×1 tube wxya 2 o

1mL×1 tube

[0049] Wherein, the amplification reaction reagent is packaged in a single tube and is in the form of dry powder. The number of reagent copies is 48 copies / box.

[0050] Establishment of fluorescent MIRA...

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Abstract

The invention provides a probe and kit for detecting human adenovirus 7 and a use method of the kit. The kit is a human adenovirus 7 type nucleic acid detection kit based on an MIRA fluorescence method. The primer of the MIRA is designed according to the specific sequence of the hexon gene of the adenovirus type 7. The nucleic acid detection kit is rapid, specific and sensitive and does not need complex instruments and equipment.

Description

technical field [0001] The invention belongs to the technical field of molecular diagnostic biology, and relates to a probe for detecting human adenovirus type 7, a kit and a use method thereof. Background technique [0002] Human adenovirus (HAdV) is a non-enveloped double-stranded DNA virus. According to the infectious neutralization reaction, HAdV can be divided into 57 serotypes, and according to the homology of nucleic acid, specificity of fibrin and antigen Determinants, the 57 serotypes currently identified are divided into 7 subgenuses A to G, A, F and G mainly cause digestive tract infections and diarrhea, C, E and some B subgenuses mainly cause respiratory tract infections, and other B subgenuses mainly cause respiratory tract infections. The genus mainly causes urinary tract infections, and the subgenus D only causes keratitis and conjunctivitis. And due to the recombination and mutation of its genome, new types are constantly emerging, and some types can cause o...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/11C12Q1/70C12Q1/6844
CPCC12Q1/701C12Q1/6844C12Q2600/166C12Q2531/119C12Q2522/101C12Q2521/507C12Q2521/319C12Q2563/107C12Q2545/113
Inventor 曹现涛陆亚平魏光李彬李拼
Owner 国药(武汉)医学实验室有限公司
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