Metagenome-based human adenovirus molecular typing and tracing method and system

A technology of human adenovirus and metagenomics, applied in the field of bioinformatics, can solve the problems of inability to type and identify human adenovirus in clinical samples, long sequencing reads, and inability to provide typing identification, etc.

Active Publication Date: 2021-04-20
HUGOBIOTECH BEIJING CO LTD +2
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Problems solved by technology

[0004] The present invention proposes a metagenomic-based human adenovirus molecular typing and traceability method and system to solve the current metagenomic sequencing typing of human adenovirus mentioned in the background technology requires long sequencing reads and virus in the sample The virus cont

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  • Metagenome-based human adenovirus molecular typing and tracing method and system
  • Metagenome-based human adenovirus molecular typing and tracing method and system
  • Metagenome-based human adenovirus molecular typing and tracing method and system

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[0084] Reference will now be made in detail to the exemplary embodiments, examples of which are illustrated in the accompanying drawings. When the following description refers to the accompanying drawings, the same numerals in different drawings refer to the same or similar elements unless otherwise indicated. The implementations described in the following exemplary examples do not represent all implementations consistent with the present disclosure. Rather, they are merely examples of apparatuses and methods consistent with aspects of the present disclosure as recited in the appended claims.

[0085] Human adenovirus (HAdV) belongs to the family Adenoviridae (Adenoviridae) mammalian adenovirus (Mastadenovirus), the genome length is about 34.7kb, belongs to dsDNA, and can infect a variety of mucosal tissues, such as gastrointestinal tract, respiratory tract, urinary tract, etc. Genital tract and cornucopia, etc., leading to self-limiting mucosal infection or even severe fatal...

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Abstract

The invention discloses a metagenome-based human adenovirus molecular typing and tracing method and system, and the method comprises the steps: constructing a human adenovirus typing database comprising a typing hierarchical classification system, and obtaining a classification unit and a complete genome/marker gene sequence of human adenoviruses, determining a complete genome/marker gene sequence of each classification unit of the human adenovirus, obtaining metagenome sequencing original data of a clinical sample, preprocessing the metagenome sequencing original data to obtain target metagenome data, analyzing the target metagenome data by utilizing a preset metagenome sequencing data dual-comparison annotation system, determining whether the clinical sample has a target human adenovirus classification unit or not, and when it is determined that the clinical sample has the target human adenovirus classification unit, determining a target associated feature corresponding to the target human adenovirus classification unit, and generating a human adenovirus typing identification report. Classification unit (subtype/genotype) typing identification of human adenoviruses can be carried out on clinical infection samples with low virus content.

Description

technical field [0001] The invention relates to the technical field of biological information, in particular to a method and system for molecular typing and traceability of human adenovirus based on metagenomics. Background technique [0002] Human adenovirus (HAdV) belongs to the family Adenoviridae (Adenoviridae) mammalian adenovirus (Mastadenovirus), the genome length is about 34.7kb, belongs to dsDNA, and can infect a variety of mucosal tissues, such as gastrointestinal tract, respiratory tract, urinary tract, etc. Genital tract and cornucopia, etc., leading to self-limiting mucosal infection or even severe fatal infection. There are three main envelope proteins on the surface of HAdV, which are important components of the envelope and important antigens for diagnosis. In genetics, the regions encoding these three types of proteins are the most variable regions in the virus genome, and are the hotspots of human adenovirus gene research. According to immunological, biol...

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Application Information

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IPC IPC(8): G16B50/00G16B30/00G16B40/00
Inventor 夏涵
Owner HUGOBIOTECH BEIJING CO LTD
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