Kit for rapidly and accurately detecting adenovirus type 3 and preparation method thereof
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A kit and adenovirus technology, applied in the field of rapid and accurate detection of adenovirus type 3 kit and its preparation, can solve the problems of low accuracy and complicated operation, and achieve high reliability, high sensitivity and good specificity Effect
Active Publication Date: 2020-08-14
BEIJING BEIER BIOENG
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Embodiment 1
[0079] 1. A detection kit group for adenovirus type 3, said kit comprising:
[0080] a. IgM antibody detection kit, the IgM antibody detection kit includes IgM antibody enzyme-labeled working solution, and the IgM antibody enzyme-labeled working solution includes the following final concentration components: sodium chloride 8g / L, dihydrogen phosphate Sodium 2.9g / L, sodium dihydrogen phosphate 0.2g / L, CaSein 2g / L, ProCLin300 0.5ml / L, peroxidase-labeled mouse anti-human IgM antibody (mouse anti-human IgM antibody was purchased from Hangzhou Longji Biotechnology Co., Ltd. company, the model is MS00704E) 1mg / L;
[0081] b. IgA antibody detection kit, the IgA antibody detection kit includes IgA antibody enzyme-labeled working solution, and the IgA antibody enzyme-labeled working solution includes the following final concentration components: sodium chloride 8g / L, dihydrogen phosphate Sodium 2.9g / L, sodium dihydrogen phosphate 0.2g / L, CaSein 2g / L, ProCLin300 0.5ml / L, peroxidase-lab...
Embodiment 2
[0103] 1. A kind of adenovirus type 3 detection kit group, described kit is the same as embodiment 1, difference only lies in:
[0104] ELISA plate coated with adenovirus type 3 recombinant antigen: each well is coated with 0.1 μg of recombinant antigen B.
[0105] 2. Refer to Example 1 for the preparation method of the above kit.
Embodiment 3
[0107] 1. A kind of adenovirus type 3 detection kit group, described kit is the same as embodiment 1, difference only lies in:
[0108] ELISA plate coated with adenovirus type 3 recombinant antigen: each well is coated with 0.1 μg of recombinant antigen C.
[0109] 2. Refer to Example 1 for the preparation method of the above kit.
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Abstract
The embodiment of the invention relates to the field of in-vitro detection, in particular to a kit for rapidly and accurately detecting adenovirus type 3 and a preparation method of the kit. The kit provided by the embodiment of the invention comprises an IgM antibody enzyme-labeled working solution, an IgA antibody enzyme-labeled working solution and an IgG antibody enzyme-labeled working solution. The IgG antibody enzyme-labeled working solution comprises a peroxidase-labeled anti-human IgG antibody and an ELISA plate coated with an adenovirus 3 type recombinant antigen; the adenovirus 3 type recombinant antigen comprises one or more of a recombinant antigen A, a recombinant antigen B or a recombinant antigen C. The kit (enzyme linked immunosorbent assay) provided by the invention makesup the blank in the market;, the detection results are comprehensively considered by respectively detecting adenovirus type 3 IgM, IgG and IgA antibodies in human serum or blood plasma, the detectionresults are complementary with each other, the reliability is high, the specificity is good, and the sensitivity is high; the kit can be complementary with nucleic acid detection, and is suitable forclinical diagnosis and epidemiological investigation.
Description
technical field [0001] The invention relates to the field of in vitro detection, in particular to a kit for rapidly and accurately detecting adenovirus type 3 and a preparation method thereof. Background technique [0002] Adenovirus (ADV) is a group of widely distributed DNA viruses, and it is one of the important pathogens that cause respiratory and digestive tract infections in humans. Virus infection mainly invades the human body through the upper respiratory tract and conjunctiva, causing acute pharyngitis, epidemic keratoconjunctivitis or viral primary atypical pneumonia, which has become a common pathogen in outbreaks, especially for immunocompromised patients. The most common isolates were adenovirus types 1-8, 40, and 41. Among them, adenovirus types 3 and 7 are common pathogens that cause acute respiratory infections in humans and are widely prevalent in my country. In recent years, outbreaks caused by adenovirus types 3 and 7 have occurred frequently in my count...
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