Primers and probe for rapidly and quantitatively detecting duck adenovirus type 4 and detection method and application thereof

Abstract

The invention discloses primers and a probe for rapidly and quantitatively detecting duck adenovirus type 4 and a detection method and application of the primers and the probe. The sequences of the primers are as shown in SEQ ID NO.1-2, and the sequence of the probe is as shown in SEQ ID NO.3. The fluorescent PCR method capable of rapidly and quantitatively detecting the duck adenovirus type 4 inthe clinical sample is established for the first time; the detection method is simple to operate, the whole operation process does not exceed 3 hours, the sensitivity is high, the specificity is good,the repeatability is good, quantitative analysis can be accurately and rapidly carried out with high flux, and the detection method is beneficial to popularization and application in clinical practice.

Description

Technical field [0001] The invention belongs to the field of molecular biology, and relates to primers and probes for rapid quantitative detection of duck adenovirus type 4, and detection methods and applications thereof. Background technique [0002] In recent years, with the rapid development of the waterfowl breeding industry, the number of adenovirus infections in duck flocks has increased, and with the rapid development of commercial meat and laying ducks in large-scale, intensified, and closed form, the level of duck feeding and management is relatively backward. , Keepers have low awareness of biosecurity, many farms are close to each other, and there are polyculture phenomena, which makes various pathogens cross-spread between different hosts, resulting in a complex and diverse disease of ducks. Among them, duck-derived avian adenovirus has a high detection rate, which seriously affects production and attracts social attention. [0003] Avian adenovirus infection is mainly...

AI Technical Summary

Problems solved by technology

But there is still no detection method for duck adenovirus type 4

At present, there is no clear information about the prevalence of the virus in the field. Therefore, it is of great significance to provide an effective and rapid method for detecting duck adenovirus type 4 in clinical samples for the monitoring and prevention of this emerging disease.

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