Primers and probe for rapidly and quantitatively detecting duck adenovirus type 4 and detection method and application thereof

A technique for quantitative detection of adenovirus, applied in the field of molecular biology, can solve problems such as the lack of detection methods for duck adenovirus type 4, and achieve good specificity, good repeatability, and strong specificity

Pending Publication Date: 2020-11-13
INST OF ANIMAL HEALTH GUANGDONG ACADEMY OF AGRI SCI
View PDF5 Cites 5 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But there is still no detection method for duck adenovirus type 4
At present, there is no clear information about the prevalence of the virus in the field. Therefore, it is of great significance to provide an effective and rapid method for detecting duck adenovirus type 4 in clinical samples for the monitoring and prevention of this emerging disease.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Primers and probe for rapidly and quantitatively detecting duck adenovirus type 4 and detection method and application thereof
  • Primers and probe for rapidly and quantitatively detecting duck adenovirus type 4 and detection method and application thereof
  • Primers and probe for rapidly and quantitatively detecting duck adenovirus type 4 and detection method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0049] In order to make the purpose, technical solutions and technical effects of the present invention clearer, the present invention will be further described in detail below in conjunction with specific embodiments. It should be understood that the specific embodiments described in this specification are only for explaining the present invention, not for limiting the present invention.

[0050] The experimental materials and reagents used, unless otherwise specified, are all conventional consumables and reagents that can be obtained commercially.

[0051] Reagents and instruments

[0052] qPCR Probe Master Mix (purchased from Nanjing Novena, Cat No.Q112-02).

[0053] The reagents used in this application are analytical reagents or biochemical reagents, and the experimental water meets the specifications of first-grade water in GB / T6682. All reagents are aliquoted in containers without DNA enzyme contamination.

[0054] The fluorescence quantitative PCR instrument is LightCycler96,...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses primers and a probe for rapidly and quantitatively detecting duck adenovirus type 4 and a detection method and application of the primers and the probe. The sequences of the primers are as shown in SEQ ID NO.1-2, and the sequence of the probe is as shown in SEQ ID NO.3. The fluorescent PCR method capable of rapidly and quantitatively detecting the duck adenovirus type 4 inthe clinical sample is established for the first time; the detection method is simple to operate, the whole operation process does not exceed 3 hours, the sensitivity is high, the specificity is good,the repeatability is good, quantitative analysis can be accurately and rapidly carried out with high flux, and the detection method is beneficial to popularization and application in clinical practice.

Description

Technical field [0001] The invention belongs to the field of molecular biology, and relates to primers and probes for rapid quantitative detection of duck adenovirus type 4, and detection methods and applications thereof. Background technique [0002] In recent years, with the rapid development of the waterfowl breeding industry, the number of adenovirus infections in duck flocks has increased, and with the rapid development of commercial meat and laying ducks in large-scale, intensified, and closed form, the level of duck feeding and management is relatively backward. , Keepers have low awareness of biosecurity, many farms are close to each other, and there are polyculture phenomena, which makes various pathogens cross-spread between different hosts, resulting in a complex and diverse disease of ducks. Among them, duck-derived avian adenovirus has a high detection rate, which seriously affects production and attracts social attention. [0003] Avian adenovirus infection is mainly...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/70C12Q1/6851C12N15/11
CPCC12Q1/701C12Q1/6851C12Q2600/166C12Q2531/113C12Q2561/101C12Q2545/114
Inventor 黄允真张俊勤孙敏华董嘉文李林林张建峰
Owner INST OF ANIMAL HEALTH GUANGDONG ACADEMY OF AGRI SCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap