The invention discloses a fast detection primer, reagent kit and method for channel catfish viruses and an application of the fast detection primer, the reagent kit and the method, and belongs to thefield of a biological technology. The method comprises steps of designing a PCR primer and a probe, preparing a DNA template, performing PCR amplification and performing result judging through luminescence signals. In accordance with gene conserved sequences corresponding to the channel catfish viruses, the primer and a taqMan probe are designed, and through sufficient optimization, the primer probe and the reaction condition are originally created, so that the method for quickly and reliably detecting the channel catfish viruses through fluorescent quantitation PCR is established. According to the fast detection primer, reagent kit and method, quantification of the DNA template is realized, and the fast detection primer, the reagent kit and the method have the advantages of being simple to operate, quick to detect, good in specificity, high in sensitivity, reliable in results and the like, and can exert important effects in respects of early rapid diagnosis and real-time monitoring ofthe channel catfish viruses.