30results about How to "No amplification" patented technology

The invention relates to a kit for rapidly detecting pathogeny of acute hepatopancreatic necrosis disease of prawns and application of the kit, belonging to technical field of biotechnology. The kit comprises an LAMP primer group, DNA polymerase, LAMP reaction liquid, a positive control and a negative control. The kit further comprises a color developing agent. The LAMP primer group comprises a pair of outer primers, a pair of inner primers and a pair of loop primers. The application of the kit in the pathogeny detection of the acute hepatopancreatic necrosis disease of the prawns comprises the steps of purifying and separating vibrio strains, preparing vibrio DNA, carrying out constant temperature gene amplification reaction, and carrying out result judgment by virtue of a turbidity meter or an ESE-Quant Tube Scanner. The kit has the advantages of good specificity, high sensitivity, simple operation, reliable result and the like, and is applicable to the rapid detection of prawn farming sites.

The invention dislcoses an RAA (recombinase-aid amplification) constant-temperature fluorescence detection method and a detection kit for EHP (enterocytozoon hepatopenaei). The detection kit comprisesa forward primer SEQ ID NO. 1, a reverse primer SEQ ID NO. 2, a specific fluorescence probe SEQ ID NO. 3, reaction liquid, recombinant polymerase and a reference substance. The kit has the advantagesthat the specificity is strong; the detection sensitivity is high, and can reach 100fg / mu L; high accuracy and reliability are achieved; the operation is simple and rapid, and the kit is suitable forfield detection and has wide application scenarios.

The invention discloses a method for preparing a RNase-resistant dengue virus nucleic acid detection quality control product. An armored RNA quality control product carrying a histidine tag and a dengue virus 3'URT gene is prepared by utilizing an MS2 phage armor technology and a site-directed mutagenesis technology; a dengue virus armored RNA quality control product is obtained through nickel column affinity chromatography purification, the quality control product is easy to purify, stable, high in concentration and free of biological infectivity, resists RNase, and can be used for carrying out quality control on the whole processes of virus detection, reverse transcription, PCR and the like in dengue virus nucleic acid detection.

The invention discloses an RAA constant temperature fluorescence detection method and a detection kit for yellow head virus (YHV) of shrimps. The detection kit comprises a forward primer SEQ ID NO.1,a reverse primer SEQ ID NO.2, a specific fluorescent probe SEQ ID NO.3, a reaction solution, reverse transcriptase, recombinant polymerase and a reference substance. The kit disclosed by the inventionhas the advantages of high specificity, high detection sensitivity as high as 0.10fg / mu L, high accuracy, reliability, simplicity and rapidness in operation, suitability for field detection and broadapplication prospect.

The invention discloses primers and a probe for rapidly and quantitatively detecting duck adenovirus type 4 and a detection method and application of the primers and the probe. The sequences of the primers are as shown in SEQ ID NO.1-2, and the sequence of the probe is as shown in SEQ ID NO.3. The fluorescent PCR method capable of rapidly and quantitatively detecting the duck adenovirus type 4 inthe clinical sample is established for the first time; the detection method is simple to operate, the whole operation process does not exceed 3 hours, the sensitivity is high, the specificity is good,the repeatability is good, quantitative analysis can be accurately and rapidly carried out with high flux, and the detection method is beneficial to popularization and application in clinical practice.

The invention discloses an LAMP detection primer group and a detection kit for white spot syndrome virus (WSSV). The detection primer group comprises a pair of outer primers, a pair of inner primers and a pair of loop primers; the detection kit comprises a primer liquid, a reaction liquid, a DNA (Deoxyribose Nucleic Acid) polymerase and a reference substance; the kit also comprises a color developing agent. The kit disclosed by the invention is simple and quick to operate and suitable for field detection; the specificity is strong, and the detection sensitivity is high and can reach 1fg / uL; the accuracy is high and reliable, and the application prospect is broad.

The invention relates to the field of animal disease molecular biology detection methods and detection agents, in particular to an animal Torque Teno virus rapid detection primer, a kit and a detection method using a loop-mediated isothermal amplification (LAMP) technology. Multiple comparisons are carried out by using Clustal W according to two different serotype gene sequences of an animal Torque Teno virus, and conserved zones of the sequences are analyzed, an inner primer and an outer primer are respectively designed by adopting LAMP primer design software, and detection and identification of TTV1 and TTV2 serotypes are realized rapidly specifically. The animal Torque Teno virus detection method disclosed by the invention has the advantages of rapidness, high sensitivity, strong specificity, convenience for operation, low requirement of equipment, time and labor saving, easiness in observing results, no complex post-treatment, and wide application range.

The invention relates to a double PCR (Polymerase chain reaction) primer for simultaneous detection of Klebsiella pneumoniae and Aeromonas caviae and a detection method of the double PCR primer, and belongs to the technical field of biological detection. The Klebsiella pneumoniae and Aeromonas caviae can be simultaneously detected in the same reaction system by taking a Klebsiella pneumoniae ompC gene and an Aeromonas caviae ahal gene as detection genes, the sizes of amplified products are 418bp and 213bp respectively, and the detection sensitivity of the two pairs of primers is 1.0*10<3>pg / mu L and 3.3*10<2>pg / mu L respectively. The double PCR detection method provided by the invention has the advantages of high specificity, high sensitivity, simple operation and short time, and can be used for simultaneous detection of Klebsiella pneumoniae and Aeromonas caviae.

The invention relates to the technical field of gene engineering, in particular to a molecular marker for identifying tremella aurantialba and an application of the molecular marker, a primer pair ishigh in specificity, tremella aurantialba DNA derived from tremella aurantialba can be subjected to specific amplification, 635bp fragments can be amplified, and no amplification effect on other localtremella aurantialba strains exists. The molecular markers, probes and / or kits for the tremella aurantialba can be specifically designed by utilizing the primer pair, the tremella aurantialba is screened and identified, tremella aurantialba strains from the tremella aurantialba can be simply, conveniently, quickly and accurately screened by utilizing the method, and the method has important significance for genetic research and market popularization of the tremella aurantialba strains.

The invention discloses recombinase-aid amplification (RAA) constant-temperature fluorescence detection method and reagent for Koi herpesvirus (KHV). The RAA constant-temperature fluorescence detection reagent for the KHV comprises a forward primer SEQ ID NO. 1, a reverse primer SEQ ID NO. 2, a specific fluorescent probe SEQ ID NO. 3, a reaction solution, recombinant polymerase and a reference substance. The RAA constant-temperature fluorescence detection reagent for the KHV disclosed by the invention is high in specificity, high in detection sensitivity (up to 100fg / microliter), high in accuracy and reliable; and moreover, the reagent is easy and convenient to operate as well as suitable for field testing. Thus, wide range of application scenarios is ensured.

The invention provides a three-way impact acceleration sensor installation mode validity test system and method. The system comprises a test ball, an excitation ball, a transmitting device, a data acquisition system and a sensor installation validity analysis unit. The three-way impact acceleration sensor is mounted on the inspection ball; the excitation ball is used for impacting and exciting the inspection ball; the launching device provides initial momentum for the excitation ball, so that the excitation ball is obliquely shot to the inspection ball, and the three-direction property and repeatability of excitation are ensured; the data acquisition system is used for receiving and storing impact time domain data; and the sensor installation validity analysis unit is used for analyzing and processing the impact data. The system and method have the beneficial that the emission device obliquely emits the excitation ball at a controllable speed and collides with the inspection ball, and after the sensor installation validity analysis unit processes impact time domain data collected by the sensor, whether the three-way impact acceleration sensor is effective in a spacecraft impact response test in the installation mode is judged.

The invention discloses a RAA constant temperature fluorescence detection method and a detection kit for a salmonid alphavirus (SAV). The detection kit comprises a forward primer SEQ ID NO.1, a reverse primer SEQ ID NO.2, a specific fluorescent probe SEQ ID NO.3, a reaction liquid, reverse transcriptase, recombinant polymerase and a reference substance. The kit disclosed by the invention is high in specificity, high in detection sensitivity which can reach 1.93 fg / [mu]L, high in accuracy, reliable, simple and rapid to operate and suitable for field detection, and has wide application scenes.

The invention discloses a prawn covert mortality nodavirus (CMNV) RAA constant temperature fluorescence detection method and a prawn covert mortality nodavirus (CMNV) detection kit, wherein the detection kit comprises a forward primer SEQ ID NO.1, a reverse primer SEQ ID NO.2, a specific fluorescent probe SEQ ID NO.3, a reaction solution, reverse transcriptase, recombinant polymerase and a controlsubstance. According to the present invention, the kit has characteristics of strong specificity, high detection sensitivity, high accuracy, reliability, simple and rapid operation and wide application scene, and is suitable for on-site detection, wherein the detection sensitivity can reach 100 fg / [mu]L.

The invention discloses an RAA constant-temperature fluorescence detection method and kit for infectious spleen and kidney necrosis virus (ISKNV) in mandarin fishes. The detection kit comprises a forward primer SEQ ID NO.1, a reverse primer SEQ ID NO.2, a specific fluorescent probe SEQ ID NO.3, a reaction solution, recombinant polymerase and a reference substance. The kit disclosed by the invention is strong in specificity, high in detection sensitivity which can reach 300 fg / [mu]L, high in accuracy, reliable, simple, convenient and rapid to operate, suitable for field detection and wide in application prospect.

The invention discloses an LAMP detection primer group for Siniperca chuatsi-perch rhabdovirus, application of the LAMP detection primer group and a detection kit. The primer group comprises a pair ofouter primers, a pair of inner primers and a pair of loop primers; the outer primers comprise a sequence L-F3 and a sequence L-B3, the sequence L-F3 of the outer primers is shown in SEQ ID NO: 1, andthe sequence L-B3 of the outer primers is shown in SEQ ID NO: 2; the inner primers comprise a sequence L-FIP and a sequence L-BIP, the sequence L-FIP of the inner primers is shown in SEQ ID NO: 3, and the sequence L-BIP of the inner primers is shown in SEQ ID NO: 4; and the loop primers comprise a sequence L-LF and a sequence L-LB, the sequence L-LF of the loop primers is shown in SEQ ID NO: 5, and the sequence L-LB of the loop primers is shown in SEQ ID NO: 6. The invention further provides application of the primer group in preparation of a Siniperca chuatsi-perch rhabdovirus detection kitand the kit. The primer group and kit, provided by the invention, have the advantages of simplicity in operation, high detection speed, good specificity, high sensitivity, reliable result, and the like.

The invention discloses a carp edema virus (CEV) RAA constant-temperature fluorescence detection method and a detection kit. The detection kit comprises a forward primer SEQ ID NO. 1, a reverse primerSEQ ID NO. 2, a specific fluorescent probe SEQ ID NO. 3, reaction liquid, recombinant polymerase and a reference substance. The kit of the invention has strong specificity; the detection sensitivityis high and can reach 2.02 fg / mu L; the accuracy is high and reliable; and the detection kit is simple, convenient and quick to operate, is suitable for field detection, and has wide application scenes.

The invention provides a method for detecting American ginseng species in traditional Chinese medicine ( Panax quinquefolius ) Recombinase-aid Amplification (RAA) constant temperature detection method and detection kit. The detection kit includes a forward primer of SEQ ID NO.1, a reverse primer of SEQ ID NO.2, a specific fluorescent probe of SEQ ID NO.3, a reaction solution, a recombinant polymerase and a control substance. The kit of the invention has strong specificity; high detection sensitivity, which can reach 0.10fg / μL; high accuracy and reliability; simple and quick operation, suitable for on-site detection, and has wide application scenarios.

The invention discloses a loop-mediated isothermal amplification (LAMP) detection primer group of aeromonas hydrophila. The loop-mediated isothermal amplification (LAMP) detection primer group comprises a pair of outer primers AH-F3 and AH-B3, a pair of inner primers AH-FIP and AH-BIP, and a pair of loop primers AH-LF and AH-LB, wherein nucleotide sequence of AH-F3 is represented by SEQ ID No.1, nucleotide sequence of AH-B3 is represented by SEQ ID No.2, nucleotide sequence of AH-FIP is represented by SEQ ID No.3, nucleotide sequence of AH-BIP is represented by SEQ ID No.4, nucleotide sequence of AH-LF is represented by SEQ ID No.5, and nucleotide sequence of AH-LB is represented by SEQ ID No.6. The invention also discloses a kit. The kit is convenient and fast for operation, is suitable for on-site detection, and is high in specificity, detection sensitivity, accuracy, and reliability; detection sensitivity can be as high as 46fg / ml; and application prospect is promising.

The invention discloses an LAMP (loop-mediated isothermal amplification) detection primer group, a kit and a detection method for letalurus punetaus herpesviruses and belongs to the field of disease prevention and control in aquaculture. The kit consists of an LAMP primer group, DNA (deoxyribonucleic acid) polymerase, an LAMP reaction liquid, positive control, negative control and a developing agent, wherein the LAMP primer group comprises a pair of outer primers, a pair of inner primers and a pair of loop primers. The detection kit comprises the following application steps in detection on theletalurus punetaus herpesviruses: preparing DNA of the letalurus punetaus herpesviruses, performing a constant-temperature gene amplification reaction and performing result judgment by using a real-time fluorescence quantitative PCR (polymerase chain reaction) instrument. The kit disclosed by the invention has the advantages of being simple to operate, rapid in detection, good in specificity, high in sensitivity, reliable in result, and the like, can play roles in early-stage rapid diagnosis and real-time monitoring on channel catfish virus diseases, and is applicable to rapid detection on pathogens on a letalurus punetaus breeding site.

The invention discloses a black fungus strain specific molecular marker primer, a test kit and application thereof. The primer pair is named as 3-ty026-3; the sequence of an upstream primer of the primer pair is 5 '-CGAGAGGCAAGGTGAACTCC-3', and the sequence of a downstream primer of the primer pair is 5 '-GCTGGCAGTCGATCAAGATC-3'. The molecular marker primer pair 3-ty026-3 is high in specificity, genome DNA of the black fungus strain Guiyun No.3 can be specifically amplified, the amplification effect on other black fungus strains is avoided, and the black fungus strain Guiyun No.3 can be rapidly and accurately identified through the specific molecular marker primer pair 3-ty026-3.

This application provides a system and method for checking the validity of the installation method of a three-way impact acceleration sensor, including a test ball, an excitation ball, a launcher, a data acquisition system, and a sensor installation effectiveness analysis unit; a three-way impact acceleration sensor is installed on the test ball; The excitation ball is used to impact the excitation test ball; the launch device provides the initial momentum for the excitation ball, so that the excitation ball shoots obliquely to the inspection ball, so as to ensure the three-way and repeatability of the excitation; the data acquisition system is used to receive and store the impact time domain data; the sensor installation effectiveness analysis unit is used to analyze and process the impact data. The beneficial effects of the application are: the launching device obliquely shoots the excitation ball at a controllable speed and collides with the inspection ball, and uses the sensor installation effectiveness analysis unit to process the impact time domain data collected by the sensor to judge the three-way impact acceleration Whether the sensor is effective for spacecraft shock response test in this installation mode.

The invention discloses a constant-temperature fluorescence detection method and a detection kit for Enterocystis hepatica (EHP) RAA of prawns. The detection kit includes a forward primer of SEQ ID NO.1, a reverse primer of SEQ ID NO.2, a specific fluorescent probe of SEQ ID NO.3, a reaction solution, a recombinant polymerase and a control substance. The kit of the invention has strong specificity; high detection sensitivity, which can reach 100 fg / μL; high accuracy and reliability; simple and quick operation, suitable for on-site detection, and has wide application scenarios.

The invention discloses a constant-temperature fluorescence detection method and a detection kit for shrimp infectious subcutaneous and hematopoietic necrosis virus (IHHNV) RAA. The detection kit includes a forward primer of SEQ ID NO.1, a reverse primer of SEQ ID NO.2, a specific fluorescent probe of SEQ ID NO.3, a reaction solution, a recombinant polymerase and a control substance. The kit of the invention has strong specificity; high detection sensitivity, which can reach 0.32fg / μL; high accuracy and reliability; simple and fast operation, suitable for on-site detection, and has wide application scenarios.

The invention discloses a fast detection primer, reagent kit and method for channel catfish viruses and an application of the fast detection primer, the reagent kit and the method, and belongs to thefield of a biological technology. The method comprises steps of designing a PCR primer and a probe, preparing a DNA template, performing PCR amplification and performing result judging through luminescence signals. In accordance with gene conserved sequences corresponding to the channel catfish viruses, the primer and a taqMan probe are designed, and through sufficient optimization, the primer probe and the reaction condition are originally created, so that the method for quickly and reliably detecting the channel catfish viruses through fluorescent quantitation PCR is established. According to the fast detection primer, reagent kit and method, quantification of the DNA template is realized, and the fast detection primer, the reagent kit and the method have the advantages of being simple to operate, quick to detect, good in specificity, high in sensitivity, reliable in results and the like, and can exert important effects in respects of early rapid diagnosis and real-time monitoring ofthe channel catfish viruses.

The invention discloses a black fungus strain specific molecular marker primer, a test kit and application thereof. The primer pair is named as 3-ty026-3; the sequence of an upstream primer of the primer pair is 5 '-CGAGAGGCAAGGTGAACTCC-3', and the sequence of a downstream primer of the primer pair is 5 '-GCTGGCAGTCGATCAAGATC-3'. The molecular marker primer pair 3-ty026-3 is high in specificity, genome DNA of the black fungus strain Guiyun No.3 can be specifically amplified, the amplification effect on other black fungus strains is avoided, and the black fungus strain Guiyun No.3 can be rapidly and accurately identified through the specific molecular marker primer pair 3-ty026-3.

The invention discloses a shrimp iridescent virus (SIV) RAA constant temperature fluorescence detection method and a detection kit. The detection kit includes a forward primer of SEQ ID NO.1, a reverse primer of SEQ ID NO.2, a specific fluorescent probe of SEQ ID NO.3, a reaction solution, a recombinant polymerase and a control substance. The kit of the invention has strong specificity; high detection sensitivity, which can reach 2fg / μL; high accuracy and reliability; simple and fast operation, suitable for on-site detection, and has wide application scenarios.

The invention discloses a WSSV prawn white spot syndrome virus (WSSV) LAMP detection primer set and a detection kit. The detection primer set includes a pair of outer primers, a pair of inner primers and a pair of loop primers; the detection kit includes a primer solution, a reaction solution, DNA polymerase and a reference substance; the kit also includes a chromogenic reagent. The kit of the invention is easy and quick to operate, suitable for on-site detection, strong in specificity, high in detection sensitivity up to 1 fg / μL, high in accuracy and reliability, and has wide application prospects.

The invention discloses PCR (polymerase chain reaction) detection primers for a drug resistance gene NDM-1 of bacteria and a detection kit containing the primers. Specific PCR primers of the gene NDM-1 are designed to investigate whether drug resistance bacteria in a farm carries the NDM-1 gene or not, the PCR reaction system and condition are optimized to obtain a PCR detection method with high sensitivity, high specificity and high repeatability for a (the) drug resistance gene NDM-1 of bacteria; and the lowest genome DNA (deoxyribonucleic acid) concentration, which can be detected by the detection method, is 9.18*10<-7>mu g / mu L. The method can be used in early diagnosis and quick screen of drug resistance gene NDM-1 carrying bacteria, epidemiological assessment as means for early diagnosis and quick screen of drug resistance gene NDM-1 carrying bacteria, epidemiological assessment,etc. in livestock and poultry breeding process, and can be applied to monitoring, prevention and control of clinical drug resistance bacteria in current veterinarian.