58 results about "Kidney necrosis" patented technology

The invention discloses a method for visually detecting mandarin fish iridovirus and infectious spleen and kidney necrosis virus based on RPA-CRISPR Cas12a / Cas13a, and belongs to the technical field of biological diagnosis. According to the method, a CRISPR / Cas system is combined with RPA amplification, a fluorescent reporter and a test strip are used for naked eye observation, and the method combines the advantages of different isothermal amplification technologies, has the advantages of short detection time, high specificity, no cross reaction, high sensitivity and the like, realizes rapid and accurate diagnosis of the two pathogens, and has a good application prospect. Important technical support is provided for prevention and control of mandarin fish diseases.

The invention discloses a primer and probe combination for detecting an infectious spleen and kidney necrosis virus and a rapid detection product, and relates to the technical field of biology. The primer and probe combination comprises an upstream primer of which the nucleotide sequence is shown as SEQ ID NO.6, a downstream primer of which the nucleotide sequence is shown as SEQ ID NO.7 and a probe of which the nucleotide sequence is shown as SEQ ID NO.17. The method has the advantages of high detection sensitivity and strong specificity. The method is adaptive to various basic layer detection scenes such as pond openings and ports, meanwhile, a complex sample pretreatment process is avoided, complex instruments and professional technologies are not needed in operation, integrated diagnosis of on-site sampling, rapid detection and instant interpretation can be achieved, and an efficient, accurate and convenient epidemic disease prevention and control technical support is provided for the aquaculture industry.