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54 results about "Kidney necrosis" patented technology

Sum of the morphological changes indicative of kidney cell death and caused by the progressive degradation action of enzymes; may affect all or part of the kidney.

Siniperca chuatsi infectious spleen and kidney necrosis virus (ISKNV) inactivation rapid detection kit and detection method

The invention discloses a siniperca chuatsi infectious spleen and kidney necrosis virus (ISKNV) inactivation rapid detection kit and a detection method. The detection kit and the detection method can monitor weather ISKNV is inactivated through detection of mRNA virus, specific steps are as follows: 7 days after an inactivated vaccine semi-finished product or finished product is inoculated with CPB cells, total cellular RNA is extracted, DNA residues are removed, reverse transcription is performed, a specific primer and a specific probe designed according to a siniperca chuatsi infectious spleen and kidney necrosis virus (ISKNV) ORF099 gene conservation region sequence are used for fluorescence quantitative PCR reaction, and weather the ISKNV is completely inactivated can be determined according to a reaction result. The detection kit and the detection method can be used for rapid detection of siniperca chuatsi infectious spleen and kidney necrosis virus, can simplify inactivation inspection operation procedures of the ISKNV cell inactivated vaccine, shortens the test cycle, saves the cost, improves the sensitivity of the inactivation inspection, and improves vaccine production efficiency.
Owner:PEARL RIVER FISHERY RES INST CHINESE ACAD OF FISHERY SCI

Proliferation method of siniperca chuatsi infectious spleen and kidney necrosis virus (ISKNV)

The invention discloses a proliferation method of a siniperca chuatsi infectious spleen and kidney necrosis virus (ISKNV). According to the proliferation method, synchronous virus inoculation is adopted, the virus and a siniperca chuatsi brain tissue cell line (CPB) are in a suspended state, and the mutual contact surface area between the virus and the siniperca chuatsi brain tissue cell line is large so that the virus enters cells through more space receptors; and the virus infects the cells while the cells differentiate and proliferate, thus obtaining the high content ISKNV. After the CPB is cultured for 30 hours to 60 hours, the CPB is transferred to 5%-8% v/v of fetal calf serum culture medium, and the ISKNV is synchronously inoculated at the MOI (Multiplicity of Infection) value of 0.85-2.3, thus obtaining a low-cost ISKNV proliferation method. After the CPB is cultured for 20 hours to 40 hours, the CPB is transferred to 8%-10% v/v of fetal calf serum culture medium, and the ISKNV is synchronously inoculated at the MOI (Multiplicity of Infection) value of 30-45, thus obtaining a short ISKNV proliferation method. Cultivation methods can be selected according to existing conditions. The proliferation method provides theoretical basis for production of low-cost viruses.
Owner:PEARL RIVER FISHERY RES INST CHINESE ACAD OF FISHERY SCI

Determination method of effective antigen contents of infectious spleen and kidney necrosis virus inactivated vaccine and kit

The invention discloses a determination method of effective antigen contents of an infectious spleen and kidney necrosis virus inactivated vaccine. According to the method, an active antigen in a to-be-tested sample is captured by taking a monoclonal antibody which is capable of recognizing ISKNV virus major capsid protein (MCP) as a capture antibody; and the captured effective antibody is quantified by taking another monoclonal antibody which is capable of recognizing different antigen epitopes of the infectious spleen and kidney necrosis virus MCP as a detection antibody. The method and a kit adopting the method provided by the invention have the advantages of being high in sensitivity, broad in linear range, convenient to use, short in required time and the like; the method is applicable to determination of effective antigen contents in a semi-finished product and a finished product in an infectious spleen and kidney necrosis virus inactivated vaccine production process; therefore,a novel method is provided for the quality control of the infectious spleen and kidney necrosis virus inactivated vaccine; and the quality of the vaccine can be guaranteed, and the production efficiency of the vaccine can be improved.
Owner:PEARL RIVER FISHERY RES INST CHINESE ACAD OF FISHERY SCI

Establishing method of myosatellite cell system of bastard halibut during embryo period

ActiveCN108753703AOvercome dysfunctional issuesCultivate suitableCell dissociation methodsCulture processFunctional genesSeawater
The invention relates to a marine fish embryo cell culture technology, in particular to an establishing method of a myosatellite cell system of bastard halibut during an embryonic period. The establishing method comprises the following steps: carrying out in-vitro separation on an embryo during a bastard halibut kirschner capsule formation period, removing an egg membrane, dispersing cells, and carrying out primary culture and secondary culture, thus establishing the myosatellite cell system during the embryonic period. The form of the myosatellite cell system, established by the invention, ofthe bastard halibut is fusiform or spindle-shaped mononuclear cells; myofiber can be differentiated to form by culturing for 5 days or above within a single generation or carrying out induction of anexogenous factor (horse serum). The myosatellite cell system is capable of supplying a large amount of myosatellite cells, and GFD (Green Fluorescent Protein) transfection and cynoglossus semilaevisspleen and kidney necrosis virus challenge assay detection verify that the myosatellite cells can be normally transfected or infected, so that the myosatellite cell system not only can be directly used for researching functional genes related to muscle growth and development of fishes and providing research materials for exploring a molecular mechanism for induction, proliferation and differentiation of muscle cells of the fishes, but also is capable of providing a platform for improved research and application of muscle characters in bastard halibut breeding.
Owner:INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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