Proliferation method of siniperca chuatsi infectious spleen and kidney necrosis virus (ISKNV)

Abstract

The invention discloses a proliferation method of a siniperca chuatsi infectious spleen and kidney necrosis virus (ISKNV). According to the proliferation method, synchronous virus inoculation is adopted, the virus and a siniperca chuatsi brain tissue cell line (CPB) are in a suspended state, and the mutual contact surface area between the virus and the siniperca chuatsi brain tissue cell line is large so that the virus enters cells through more space receptors; and the virus infects the cells while the cells differentiate and proliferate, thus obtaining the high content ISKNV. After the CPB is cultured for 30 hours to 60 hours, the CPB is transferred to 5%-8% v / v of fetal calf serum culture medium, and the ISKNV is synchronously inoculated at the MOI (Multiplicity of Infection) value of 0.85-2.3, thus obtaining a low-cost ISKNV proliferation method. After the CPB is cultured for 20 hours to 40 hours, the CPB is transferred to 8%-10% v / v of fetal calf serum culture medium, and the ISKNV is synchronously inoculated at the MOI (Multiplicity of Infection) value of 30-45, thus obtaining a short ISKNV proliferation method. Cultivation methods can be selected according to existing conditions. The proliferation method provides theoretical basis for production of low-cost viruses.

Description

technical field

[0001] The invention relates to a propagation method of mandarin fish infectious spleen and kidney necrosis virus ISKNV. Background technique

[0002] Mandarin fish (Siniperca chuatsi) is one of the important freshwater characteristic aquaculture species in my country. In recent years, the iridescent virus disease of mandarin fish caused by Infectious spleen and kidney necrosis virus (ISKNV) has caused serious damage to the mandarin fish aquaculture industry. serious economic loss. In 1997, Wu Shuqin and others first discovered a spherical virus with a diameter of 150nm in sick mandarin fish, and considered it to be the pathogen of the fulminant infectious disease of mandarin fish. In 1998, He Jianguo and others further proved the pathogenicity of the virus through recurrent infection. Through histological studies, it was found that the spleen and kidney of mandarin fish were the main organs infected by it, and it was named infectious spleen and kidney necros...

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