PCR primer group for rapidly detecting three fish viruses and application thereof

A primer set and virus technology, applied in the field of gene detection, can solve the problems of easy cross-infection, time-consuming and labor-intensive, and achieve the effects of high sensitivity, improved efficiency and strong specificity
CN112359148AActive Publication Date: 2021-02-12PEARL RIVER FISHERY RES INST CHINESE ACAD OF FISHERY SCI
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
PEARL RIVER FISHERY RES INST CHINESE ACAD OF FISHERY SCI
Publication Date
2021-02-12

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Abstract

The invention discloses a triple PCR primer group for rapidly detecting three fish viruses and application thereof, and relates to the technical field of gene detection. The invention discloses a triple PCR primer group for rapidly detecting three fish viruses. The triple PCR primer group comprises an infectious spleen and kidney necrosis virus ISKNV primer, a mandarin fish frog virus SCRIV primerand a mandarin fish rhabdovirus SCRV primer, the triple PCR primer group has high sensitivity, the detection time is 2-3 hours earlier than that of conventional PCR, the efficiency of clinical diagnosis is greatly improved, richer reference bases can be provided for epidemiological investigation of ISKNV, SCRIV and SCRV infection, and the triple PCR primer group has important significance for subsequent research and prevention and treatment.
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Description

technical field

[0001] The invention relates to the technical field of gene detection, in particular to a PCR primer set for rapid detection of three fish viruses and its application. Background technique

[0002] Mandarin fish has a long history of breeding and is one of the high-quality freshwater fish in my country. It is loved by consumers for its high yield, tender meat, less spines, sweet taste, and rich protein. However, with the rapid expansion and development of mandarin fish farming technology, equipment and feed market in China, Infectious spleen and kidney necrosis virus (ISKNV), mandarin fish frog virus (Siniperca chuatsi ranairidovirus, SCRIV) and mandarin shellfish Viral diseases such as Siniperca chuatsi rhabdovirus (SCRV) have become the main factors restricting the sustainable development of mandarin fish aquaculture. ISKNV is one of the main pathogens of large-scale outbreaks of infectious diseases in mandarin fish, and its clinical symptoms usually manif...

Claims

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