Method and kit for measuring effective antigen content of inactivated vaccine against infectious spleen and kidney necrosis virus

A technology of spleen-kidney necrosis virus and inactivated vaccine, which is applied in the field of determination method and kit for the effective antigen content of inactivated vaccine of infectious spleen-kidney necrosis virus, can solve the problems of practicability and biosafety, virus diffusion, antigen quantification, etc. , to achieve the effect of improving vaccine production efficiency, ensuring vaccine quality, and shortening the time
CN107974434BActive Publication Date: 2021-06-11PEARL RIVER FISHERY RES INST CHINESE ACAD OF FISHERY SCI
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
PEARL RIVER FISHERY RES INST CHINESE ACAD OF FISHERY SCI
Publication Date
2021-06-11

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Abstract

The invention discloses a method for determining the effective antigen content of an infectious spleen and kidney necrosis virus inactivated vaccine. The method uses a monoclonal antibody that can recognize the main capsid protein (MCP) of the ISKNV virus as a capture antibody to capture in the sample to be tested. The effective antigen of the captured effective antigen was quantified by using another monoclonal antibody that recognizes different epitopes of infectious spleen and kidney necrosis virus MCP as a detection antibody. The method of the present invention and the kit using the method have the advantages of high sensitivity, wide linear range, convenient use, and short time required, and can be used for effective antigens in semi-finished products and finished products in the production process of infectious spleen and kidney necrosis virus inactivated vaccines. The determination of the content provides a new method for the quality control of the inactivated vaccine of infectious spleen and kidney necrosis virus, which can ensure the quality of the vaccine and improve the production efficiency of the vaccine.
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Description

technical field

[0001] The invention relates to a method for measuring the effective antigen content of a vaccine, in particular to a method for measuring the effective antigen content of an infectious spleen and kidney necrosis virus inactivated vaccine and a kit. Background technique

[0002] Mandarin fish (Siniperca chuatsi) is an important species of high-quality freshwater fish consumption and export earnings in my country. It is deeply loved by consumers because of its delicious taste and high protein content. However, the serious disease problem has become the main bottleneck restricting the development of mandarin fish aquaculture. Since 1997, the outbreak of infectious diseases of mandarin mandarin fish has brought huge economic losses to the mandarin fish aquaculture. Wu Shuqin and others confirmed for the first time that a large spherical virus particle with a hexagonal cross section and a diameter of about 150nm is the main pathogen of the fulminant infectious di...

Claims

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