Infectious spleen and kidney necrosis virus (ISKNV) gene intron and its use in distinguishing of live and inactivated ISKNVs

An intron, live virus technology, applied in double-stranded DNA viruses, viruses, viruses/phages, etc., to achieve high sensitivity, high sensitivity, and shorten the inactivation test period.
CN106520769AActive Publication Date: 2017-03-22PEARL RIVER FISHERY RES INST CHINESE ACAD OF FISHERY SCI
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
PEARL RIVER FISHERY RES INST CHINESE ACAD OF FISHERY SCI
Publication Date
2017-03-22

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Abstract

The invention discloses an infectious spleen and kidney necrosis virus (ISKNV) gene intron and its use in distinguishing of live and inactivated ISKNVs and belongs to the field of virus detection. The intron is located on ORF003L of an ISKNV genome NC_003494.1, has length of 80bp and is named as IN-3. The invention discloses the intron of ISKNV. The intron is named as IN-3. Through the characteristics of transcription shearing of the intron IN-3, the intron IN-3 is used as a virus complete inactivation index, and an ISKNV inactivation rapid detection nested RT-PCR method is established. The ISKNV gene intron can shorten an inactivation test cycle in ISKNV cell inactivated vaccine production process and improve vaccine production efficiency.
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Description

technical field

[0001] The invention belongs to the field of virus detection, in particular to ISKNV ORF003L gene intron and its application in distinguishing ISKNV live virus and inactivated virus. Background technique

[0002] Mandarin fish (Sinipercachuatsi) is an important species of high-quality freshwater fish consumption and export earnings in my country. It is deeply loved by consumers because of its delicious taste and high protein content. However, the serious disease problem has become the main bottleneck restricting the development of mandarin fish aquaculture. Since 1997, the outbreak of infectious diseases of mandarin mandarin fish has brought huge economic losses to the mandarin fish aquaculture. Wu Shuqin and others confirmed for the first time that a large spherical virus particle with a hexagonal cross section and a diameter of about 150nm is the main pathogen of the fulminant infectious disease of mandarin fish. Because the virus mainly infects the spleen...

Claims

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