14 results about "Myosatellite cell" patented technology

The invention relates to the technical field of cell culture, in particular to an oriented porous bionic gelatin scaffold for controlling cell orientation arrangement, a preparation method and application thereof, and the oriented porous bionic gelatin scaffold can be used for cell culture meat production. The preparation method comprises the following steps: mixing and solidifying gelatin and glutamine transaminase, directionally freezing, freeze-drying, sterilizing, inoculating and culturing cells and the like. Parameters and culture medium components in each step are also explained. Compared with the prior art, a regular directional porous structure is formed through a hydrogel scaffold and directional freezing, directional arrangement and growth of fish muscle satellite cells are guided, the cell activity is high, and the proliferation and differentiation effects are good; the gelatin material is adopted, so that the cost is low, the biocompatibility is good, and the gelatin material is superior to collagen and wheat glutelin; the preparation process is simple, does not need complex equipment, is suitable for large-scale production, and promotes the development of cell culture meat.

The invention discloses a skeletal muscle repair method and system based on muscle satellite cell regulation and control. The method comprises the following steps: performing proliferation and differentiation correlation analysis on satellite cell steady state data and aerobic exercise intensity data to establish a proliferation regulation and control map; determining a collaborative repair time window according to the atlas, extracting difference response deviation correction parameters to form an activity correction factor, and performing repair intensity correction on the collaborative repair time window to generate a self-adaptive repair window; carrying out exercise prescription adaptation on the self-adaptive repair window, identifying a satellite cell activation regulation and control channel, obtaining activation delay time through activation efficiency mapping, and delimiting a response level according to the activation delay time to generate layered repair configuration; activation delay evaluation is conducted on layered repair configuration to determine a preferred activation mode, dynamic activation characteristics are extracted based on optimal activation time window data to form an intervention execution sequence, a skeletal muscle repair execution instruction is output in combination with an exercise prescription parameter table, and dynamic collaborative adaptation of satellite cell repair state monitoring and training scheme parameters is achieved.

The invention belongs to the technical field of cell engineering and genetic engineering, and particularly relates to application of an FBXO32 gene in regulation and control of skeletal muscle satellite cell proliferation and feed utilization rate. According to overexpression of the FBXO32 gene in skeletal muscle satellite cells, it is found that the mRNA expression quantity of skeletal muscle satellite cell proliferation marker genes KI67 and CCND1 is remarkably increased, and the protein expression level is also remarkably improved. CCK-8 and EdU detection results also show that the FBXO32 gene or the encoded protein thereof also has a promotion effect on skeletal muscle satellite cell proliferation. The overexpressed FBXO32 gene or the encoded protein thereof can promote the proliferation of skeletal muscle satellite cells, and lays a foundation for improving meat traits of livestock and poultry, improving the feed utilization rate of the livestock and poultry and improving the meat traits of the livestock and poultry.

The invention relates to the technical field of biological breeding, in particular to application of BMP6 protein or a coding gene thereof to regulation and control of myogenic differentiation of poultry skeletal muscle satellite cells. The invention finds that the BMP6 gene is a key factor for regulating and controlling the senescence of the muscle-derived satellite cells and inducing the cells to be converted from an activated state to a resting state through the research of an snRNA-seq combined in-vivo and in-vitro method, and proves that the knock-down of the BMP6 can promote the activation and myogenic differentiation of the skeletal muscle satellite cells. The invention provides a new way for improving the meat production performance of poultry, breeding an improved variety and preventing and treating related myopathy.

In vitro method for producing meat by multiplying non-human fibroblasts, muscle cells, skeletal muscle stem cells and / or myosatellite cells by cultivation in serum-free medium, characterized in that the serum-free medium is free of blood serum and free of albumin, free of ROCK inhibitor, free of pro-inflammatory interleukins (IL), in particular free of IL-6, free of insulin-like growth factor (IGF) and free of vascular endothelial growth factor (VEGF), and the medium contains an inhibitor of the TGFβ signaling pathway which is an inhibitor of Smad2 / 3, and in that the medium contains TGFβ.

The invention belongs to the technical field of biology and cells, and discloses application of a circAAGAB gene in regulation and control of proliferation and differentiation of bovine skeletal muscle satellite cells, the circAAGAB gene truly exists and is in a ring shape, and the nucleotide sequence of the circAAGAB gene is shown as SEQ ID NO.1. The circAAGAB gene has a nucleotide sequence shown as SEQ ID NO.2. The circAAGAB gene has a nucleotide sequence shown as SEQ ID NO.1. According to the method disclosed by the invention, the proliferation and myogenic differentiation process of the bovine muscle satellite cells can be effectively regulated and controlled by regulating the expression of circAAGAB. A new enlightenment is provided for researching muscle development and differentiation, and an important reference is provided for exploring a muscle growth and development mechanism and improving the yield and quality of beef. In addition, a good foundation is laid for deeply discussing the action mechanism of circAAGAB in the muscle development process, and theoretical support is provided for rapid breeding and meat quality improvement of new varieties of high-quality beef cattle.

The present invention relates to methods for culturing one or more primary mammalian cells in suspension, and / or cells or tissues prepared in such methods, and / or compositions such as media useful in such methods. In particular aspects, the invention relates to methods of proliferating one or more primary mammalian cells, such as one or more primary mammalian myosatellite cells, fibroblasts, and / or adipose stromal cells, or progenitors thereof, in suspension culture. Related uses, and compositions suitable for use in such methods, are also provided.

The invention discloses a preparation method of an MSTN knockout bovine skeletal muscle satellite cell line based on Prime Editing. The preparation method comprises the following steps: step 1, designing and screening sgRNA; step 2, synthesis of pegRNA and construction of epiPE2 plasmids are carried out; step 3, optimizing a power conversion condition; 4, measuring the efficiency of the PE edited plasmid after electrotransformation; and 5, cell performance determination. According to the method, the bovine MSTN gene is knocked out by designing specific pegRNA and applying a Prime Editing technology, the advantages of high editing efficiency, high accuracy, high safety and the like are shown, the cell survival rate and the genome stability are improved, the bovine skeletal muscle satellite cell line with the MSTN specific site being accurately knocked out is successfully created by utilizing Primer Editing for the first time, and the bovine skeletal muscle satellite cell line with the MSTN specific site being accurately knocked out is obtained. And a basis is provided for subsequent muscle differentiation or breeding application.

This invention provides novel applications for the IGF2BP1 gene, including the use of the IGF2BP1 gene and related reagents to prepare products that promote the proliferation and differentiation of goat skeletal muscle satellite cells (MuSCs) or promote goat muscle development. This invention demonstrated the promoting effect of IGF2BP1 on the proliferation and differentiation of goat MuSCs by overexpressing and interfering with IGF2BP1 in goat MuSCs. This invention also found a significant positive correlation between the expression level of IGF2BP1 and circMYO9B, and that circMYO9B is a circRNA with m6A modification, regulated by the level of m6A modification. IGF2BP1 recognizes circMYO9B via the m6A pathway, stabilizes its expression, and thus regulates the proliferation of goat MuSCs. The technical research of this invention provides epigenetic insights into goat muscle development and further refines the molecular regulatory network of goat muscle development.

The present application relates to the technical field of genetic engineering, and particularly relates to application of ELN gene in regulating proliferation of skeletal muscle satellite cells and feed utilization rate. The present application finds that the ELN gene can regulate the proliferation of skeletal muscle satellite cells, and specifically, increasing the expression amount of the ELN gene and / or ELN protein can promote the proliferation of skeletal muscle satellite cells, and decreasing the expression amount of the ELN gene and / or ELN protein can inhibit the proliferation of skeletal muscle satellite cells. Based on this, the meat production performance and feed conversion rate of meat livestock can be improved by increasing the expression amount of the ELN gene and / or ELN protein, and a new breed of meat livestock with improved meat production performance can be bred.

The invention belongs to the technical field of biological materials, and particularly relates to skeletal muscle structure and microenvironment bionic sponge and a preparation method and application thereof. The skeletal muscle microenvironment-like decellularized ECM and a directional freezing technology are utilized to successfully construct the skeletal muscle structure and microenvironment double bionic decellularized ECM / sodium alginate sponge, the physiological skeletal muscle microenvironment can be fully simulated, and the migration, proliferation and differentiation of muscle satellite cells are effectively promoted, so that the regeneration, maturation and differentiation of muscle fibers in the sponge are promoted. Therefore, the invention can effectively solve the dilemma and limitation of fibrosis of the current decellularized ECM in VML treatment application, promote regeneration and repair of damaged tissues of the VML, provide a new treatment approach for regeneration and functional recovery of muscular tissues of the VML, lay a theoretical and technical foundation for research and development of the decellularized ECM with clinical potential application value and a scaffold thereof, and have a wide application prospect. Therefore, the method has important clinical potential significance.

The invention relates to the technical field of fish cell culture, cell differentiation regulation and control and cell culture aquatic product tissue construction, in particular to a method for regulating and controlling fish stem cell co-culture and synchronously inducing myobiogenic and adipogenic differentiation based on microcarrier matrix stiffness. Adding the fish muscle satellite cells, the fish adipose-derived stem cells and the two microcarriers into the same culture system, and performing suspension dynamic culture; the culture system at least comprises two types of microcarriers: a high-rigidity microcarrier and a low-rigidity microcarrier. According to the method, the same culture system is adopted in a single reactor to penetrate through the whole process, mechanical microenvironment difference is provided by simultaneously configuring microcarriers with different rigidities, and the mechanical microenvironment difference is provided under the conditions that a muscle formation / fat formation induction stage is not additionally set and induction factors are not added; muscular satellite cells and adipose-derived stem cells are synchronously subjected to myoblast and adipogenic differentiation in the same system, and product myolipid tissue controllability and batch-to-batch repeatability are achieved through the microcarrier proportion and the cell proportion.

The application discloses a serum-free culture method for inducing differentiation of muscle satellite cells into myotubes. The inventors have found that the muscle satellite cells can be efficiently induced to differentiate into myotubes with a differentiation rate of up to 46.2% by sequentially adding dexamethasone and insulin into a serum-free basic culture medium. The serum-free culture method has the advantages that no serum or other components with unknown compositions need to be added into the culture medium, the chemical composition of the culture medium is clear, the stability of the induced culture is good, the cost is controllable, and the method is safer.

The application discloses a skeletal muscle repair method and system based on muscle satellite cell regulation, and performs proliferation differentiation correlation analysis on satellite cell steady state data and aerobic exercise intensity data to establish a proliferation regulation map; a synergistic repair time window is determined according to the map, a difference response deviation correction parameter is extracted to form an activity correction factor, repair intensity correction is performed on the synergistic repair time window to generate a self-adaptive repair window; the self-adaptive repair window is subjected to exercise prescription adaptation and satellite cell activation regulation pathways are identified, an activation delay time is obtained through activation efficiency mapping, a response level is generated according to the activation delay time to form a layered repair configuration; a preferred activation mode is determined through activation delay evaluation on the layered repair configuration, dynamic activation characteristics are formed by extracting data of the best activation time window, skeletal muscle repair execution instructions are output in combination with an exercise prescription parameter table, and satellite cell repair state monitoring and dynamic synergistic adaptation of training scheme parameters are realized.