31 results about "Muscle satellite cell" patented technology

The invention relates to the technical field of gene engineering, in particular to application of a knock-down CDYL gene in improvement of cattle meat production performance. The invention provides an application of knocking down a CDYL gene in improving the differentiation capacity of cattle muscle satellite cells and / or improving the meat production performance of cattle. The gene ID of the CDYL gene is 539013. The 404th basic group A on the upstream of the CDYL gene transcription start site is deleted through a gene editing method, so that the CDYL knock-down gene editing cattle is created, compared with wild cattle and gene editing cattle, the myotube forming ability of muscle satellite cells is obviously enhanced, the number of myotube marker intermuscular line protein positive cells is obviously increased, the number of multinuclear myotubes is obviously increased, and the CDYL knock-down gene editing cattle has the advantages that the CDYL knock-down gene editing cattle can be used for preparing the CDYL knock-down gene editing cattle. And the weight and body size data (body height, cross height, body oblique length, chest circumference and abdominal circumference) of the gene edited cattle are greater than those of the wild cattle. According to the invention, high-yield breeding genes of beef cattle are enriched, and technical support is provided for cultivation of novel gene editing animals.

A method for co-cultivation and directed differentiation induction of muscle satellite cells and adipose-derived stem cells is provided. The method includes the steps of co-cultivation and co-differentiation. The method for co-cultivation and directed differentiation induction of muscle satellite cells and adipose-derived stem cells provided by the present disclosure can allow the effective co-cultivation and co-directed differentiation induction of muscle satellite cells and adipose-derived stem cells from Larimichthys crocea, thereby providing a feasible solution for the large-scale production of high-quality cultivated meat.

The invention relates to the technical field of cell culture, in particular to an oriented porous bionic gelatin scaffold for controlling cell orientation arrangement, a preparation method and application thereof, and the oriented porous bionic gelatin scaffold can be used for cell culture meat production. The preparation method comprises the following steps: mixing and solidifying gelatin and glutamine transaminase, directionally freezing, freeze-drying, sterilizing, inoculating and culturing cells and the like. Parameters and culture medium components in each step are also explained. Compared with the prior art, a regular directional porous structure is formed through a hydrogel scaffold and directional freezing, directional arrangement and growth of fish muscle satellite cells are guided, the cell activity is high, and the proliferation and differentiation effects are good; the gelatin material is adopted, so that the cost is low, the biocompatibility is good, and the gelatin material is superior to collagen and wheat glutelin; the preparation process is simple, does not need complex equipment, is suitable for large-scale production, and promotes the development of cell culture meat.

The invention discloses a skeletal muscle repair method and system based on muscle satellite cell regulation and control. The method comprises the following steps: performing proliferation and differentiation correlation analysis on satellite cell steady state data and aerobic exercise intensity data to establish a proliferation regulation and control map; determining a collaborative repair time window according to the atlas, extracting difference response deviation correction parameters to form an activity correction factor, and performing repair intensity correction on the collaborative repair time window to generate a self-adaptive repair window; carrying out exercise prescription adaptation on the self-adaptive repair window, identifying a satellite cell activation regulation and control channel, obtaining activation delay time through activation efficiency mapping, and delimiting a response level according to the activation delay time to generate layered repair configuration; activation delay evaluation is conducted on layered repair configuration to determine a preferred activation mode, dynamic activation characteristics are extracted based on optimal activation time window data to form an intervention execution sequence, a skeletal muscle repair execution instruction is output in combination with an exercise prescription parameter table, and dynamic collaborative adaptation of satellite cell repair state monitoring and training scheme parameters is achieved.

The present invention relates to the field of genetic engineering technology, and particularly to the application of knocking down CDYL genes in improving the meat production performance of cattle. The present invention provides the application of knocking down CDYL genes in improving the differentiation ability of bovine muscle satellite cells and / or improving the meat production performance of cattle, and the gene ID of the CDYL gene is 539013. The present invention deletes the 404th base A upstream of the transcription start site of the CDYL gene by gene editing, thereby creating a CDYL gene-edited cattle with knocked-down gene. Compared with wild-type cattle, the myotube formation ability of muscle satellite cells in gene-edited cattle is significantly enhanced, the number of desmin-positive cells, a myotube marker, is significantly increased, the number of multinucleated myotubes is significantly increased, and the body weight and body size data (body height, rump height, body slant length, chest girth and abdominal girth) of gene-edited cattle are all greater than those of wild-type cattle. The present invention enriches the breeding genes for high-yield beef cattle and provides technical support for the cultivation of new gene-edited animals.

The invention discloses a traditional Chinese medicine composition for treating sarcopenia by regulating a CXCR4 / GPX4 axis to inhibit ferroptosis and a preparation method and application thereof, and belongs to the technical field of traditional Chinese medicines. The traditional Chinese medicine composition is prepared from the following raw material medicines in parts by weight: 5-15 parts of eucommia ulmoides, 5-15 parts of herba epimedii, 5-15 parts of fructus psoraleae and 5-15 parts of semen cuscutae. The traditional Chinese medicine composition disclosed by the invention has the traditional effects of tonifying kidney, strengthening yang and strengthening tendons and bones, and is proved to be capable of promoting GPX4 expression and inhibiting ferroptosis of muscle cells by up-regulating CXCR4 expression, so that muscle strength is effectively enhanced, proliferation and differentiation of muscle satellite cells are promoted, oxidative stress is reduced, and the traditional Chinese medicine composition is used for preventing or treating sarcopenia.

A composition and a method are provided for improving number and / or function of muscle satellite cells, and / or treating or preventing muscle atrophy in a subject in need thereof. Such a composition comprising a homogenous polysaccharide or a derivative thereof, or a pharmaceutically acceptable ester or salt thereof, or a pharmaceutically acceptable solvate thereof, or any combination thereof, and a pharmaceutically acceptable excipient. The homogeneous polysaccharide consists essentially of arabinose, galactose, rhamnose, and galacturonic acid as monomer units. Such a method comprises administrating an effective amount of such a composition into a subject in need thereof.

This invention belongs to the field of biomedical technology, specifically involving the application of the MRPL41 gene / protein in the preparation of drugs for treating age-related sarcopenia. This invention is the first to demonstrate that MRPL41 is a key rate-limiting factor in the assembly of mitochondrial ribosomes in aging muscle satellite cells, establishing it as a core target for the treatment of age-related sarcopenia. Overexpression of MRPL41 can promote the assembly of mitochondrial 55S holoenzymes, improve the translation efficiency of mitochondrial-encoded proteins, repair mitochondrial dysfunction at its source, thereby promoting muscle repair, improving muscle atrophy, and achieving the treatment of age-related sarcopenia. This invention provides a precise microscopic target for the treatment of age-related sarcopenia, overcoming the limitations of existing clinical therapies (such as hormone replacement and simple nutritional supplementation) due to significant side effects and "anabolism resistance," providing a new direction for the development of innovative drugs specifically for the treatment of age-related sarcopenia, and has excellent application prospects.

The application discloses a kind of northern Shaanxi white cashmere goat muscle satellite cell separation and purification method, the present application uses northern Shaanxi white cashmere goat fetus dorsal longest muscle tissue as test material, by aseptic pretreatment, tissue block inversion adherent culture, primary subculture culture Combination of compound technology of hierarchical differential adherent purification, stably obtain the 5th generation muscle satellite cell.The present application optimizes and adapts culture system and purification process, overcomes the defects of low cell yield, serious interference of mixed cells, activity and poor purity of traditional preparation method.At the same time, the present application also adopts RT-PCR molecular identification and immunofluorescence staining combined with multi-level verification method, from gene and protein level Bidirectional verification cell specificity and differentiation potential, accurately determine the biological properties of cell.The present application process is simple, good stability and repeatability, can efficiently prepare northern Shaanxi white cashmere goat muscle satellite cell, provides important technical support for cashmere goat germplasm resource conservation, muscle development mechanism and muscle regeneration related research.

A composition of INFRA cells is described. The composition includes human muscle stem cells (SCs) expressing the transcription factor PAX7 and lacking the expression of the transmembrane receptor PDGFRA, wherein at least 70% of the population of human muscle SCs are PAX7+ and PDGFRA-. Methods of preparing the INFRA cell compositions, and methods of using the INFRA cells to treat muscle injury are also described.

The present invention belongs to the field of bioengineering technology, and in particular to a method for extracting salmon muscle satellite cells. The method comprises the following steps: (1) salmon muscle tissue; (2) PBS is used to clean the muscle tissue, and the muscle tissue is cut into small pieces with scissors; (3) the minced muscle tissue is transferred to L-15 basal medium containing antibiotics and collagenase I, and digested at room temperature; (4) the digestion solution is centrifuged; (5) the precipitate after centrifugation is added to L-15 complete medium, resuspended by blowing, and centrifuged; (6) the precipitate is resuspended and inoculated in a 6-well plate, and cultured under the conditions of 15°C to 28°C. The present invention realizes the extraction and separation and culture of salmon muscle satellite cells by adjusting the cell culture medium and optimizing the proliferation temperature, and lays a foundation for further obtaining salmon cell culture meat.

Methods for building muscle tissue in a subject are described, which comprise administering an ellagitannin compound represented by the following formula (I) to a subject in need thereof:The ellagitannin compound represented by the formula (I) may be casuarinin or stachyurin, and is administered in a therapeutically effective amount. For instance, the ellagitannin compound may be administered in an amount effective for activating muscle satellite cells. These methods increase and / or maintain muscle mass, as well as prevent and / or suppress a decrease of muscle mass and / or a decrease of muscle force. In certain embodiments, the subject is a human, such as an elderly person. In particular these methods are useful for subjects having or at risk of diseases such as sarcopenia, locomotive syndrome and / or frailty. The ellagitannin compound represented by the formula (I) may be administered as a component of a food or drink composition.

The invention discloses an application of PDGF-C (Platelet Derived Growth Factor-C) in muscle injury repair. Research finds that in a CTX model mouse with Pdgf-c knocked out, the number of proliferated muscle satellite cells (Pax7 + Ki67 +) is remarkably smaller than that of wild type mice. Through AAV-mediated PDGF-C overexpression, the number of CTX model mouse muscle stem cells can be significantly increased, the activation level of the muscle stem cells is improved, and the self-renewal ability of the muscle stem cells is enhanced. In addition, PDGF-C overexpression also significantly increases the area of muscle fibers of CTX model mice and improves the regeneration and repair ability of the muscle fibers, thereby promoting the healing of muscle injury and having a therapeutic effect on the disease. In addition, the effect of the PDGF-C on promoting muscle injury repair is obviously higher than that of the PDGF-B. Therefore, the invention provides a new method for the treatment of muscle injury, and a new way is possibly opened up for the treatment of other muscle-related diseases.

A composition of INFRA cells is described. The composition includes human muscle stem cells (SCs) expressing the transcription factor PAX7 and lacking the expression of the transmembrane receptor PDGFRA, wherein at least 70% of the population of human muscle SCs are PAX7+ and PDGFRA-. Methods of preparing the INFRA cell compositions, and methods of using the INFRA cells to treat muscle injury are also described.

This invention discloses the application of PDGF-C in muscle injury repair. The study found that in CTX model mice with PDGF-C knockout, the proliferation of muscle satellite cells (Pax7)... + Ki67 + The number of muscle stem cells in CTX model mice was significantly lower than that in wild-type mice. AAV-mediated PDGF-C overexpression significantly increased the number of muscle stem cells, improved their activation level, and enhanced their self-renewal capacity. Furthermore, PDGF-C overexpression significantly increased the surface area of ​​muscle fibers in CTX model mice, enhancing their regenerative and repair capabilities, thereby promoting muscle injury healing and demonstrating a therapeutic effect on this disease. Moreover, PDGF-C's effect on promoting muscle injury repair was significantly higher than that of PDGF-B. Therefore, this invention provides a new method for treating muscle injuries and may open new avenues for the treatment of other muscle-related diseases.

To provide a muscle satellite cell proliferation promoter and a muscle satellite cell proliferation inhibitor.SOLUTION: The present invention provides a muscle satellite cell proliferation promoter that contains at least one selected from the group consisting of soybean extract, semi-fermented tea leaf extract, and non-fermented tea leaf extract and provides a muscle satellite cell proliferation inhibitor that contains fermented tea leaf extract.SELECTED DRAWING: None

The present invention provides compositions and methods for improving the number and / or function of muscle satellite cells, and / or treating or preventing muscle atrophy in a subject in need of such therapy. The composition comprises a homogeneous polysaccharide, or a pharmaceutically acceptable ester or salt thereof, or a pharmaceutically acceptable solvate thereof, or any combination thereof, and a pharmaceutically acceptable excipient. The homogeneous polysaccharide is mainly composed of arabinose, galactose, rhamnose and galacturonic acid as monomeric units. The method comprises administering an effective dose of such a composition to a subject in need thereof.

The present invention provides compositions and methods for improving muscle satellite cell number and / or function and / or treating or preventing muscle atrophy in a subject in need of such a treatment. Such compositions include a homopolysaccharide, or a pharmaceutically acceptable ester or salt thereof, or a pharmaceutically acceptable solvate thereof, or any combination thereof, and a pharmaceutically acceptable excipient. The homopolysaccharide is mainly composed of arabinose, galactose, rhamnose, and galacturonic acid as monomer units. Such methods include administering an effective dose of such a composition to a subject in need thereof.

Compositions and methods for modulating HIF-2α to meditate of hypoxia signaling in satellite cells and applications thereof for improving skeletal muscle generation and repair are provided. For example, methods of enhancing, increasing, accelerating or / and otherwise improving skeletal muscle generation or regeneration in a subject in need thereof are disclosed. In some embodiments, the methods include administering the subject an effective amount of HIF-2α inhibitor. The HIF-2α inhibitor can be effective to, for example, increase muscle satellite cell proliferation, differentiation, or a combination thereof in a subject. Composition and methods for improving respiration, and reducing or preventing the development or progression of fibrosis are also provided. The disclosed compositions and methods are particularly useful for treating muscular dystrophies, myopathies, and other muscle-related diseases and disorders.

The present invention relates to the field of genetic engineering technology, and discloses an epigenetic gene editing system based on CRISPR / dCas12a and its application. Wherein, the epigenetic gene editing system comprises: a superCRISPRon fusion protein and a crRNA that guides the superCRISPRon fusion protein to target a target gene; the amino acid sequence of the superCRISPRon fusion protein is shown in any one of SEQ ID NO.1~6. Compared with TET1-dCas12a and TET1-dCas12a-VPR, the epigenetic gene editing system of the present invention has the characteristics of high efficiency and lasting effect. In addition, the epigenetic gene editing system of the present invention can activate the expression of follistatin (FSTN) in muscle satellite cells, improve the differentiation ability of muscle satellite cells, and thus promote muscle growth. The epigenetic gene editing system upgrades the existing tools, improves the editing efficiency and duration, and plays an important role in promoting the application of gene editing in clinical treatment.

The present invention relates to the field of genetic engineering technology, and discloses an epigenetic gene editing system based on CRISPR / dCas9 and its application. The epigenetic gene editing system comprises: a superCRISPRoff fusion protein and an sgRNA that guides the superCRISPRoff fusion protein to target a target gene; the amino acid sequence of the superCRISPRoff fusion protein is shown in any one of SEQ ID NO.1~6. Compared with the existing tools dCas9‑DNMT3A‑3L or CRISPRoff, the epigenetic gene editing system of the present invention has the characteristics of high efficiency and lasting effect. In addition, the epigenetic gene editing system of the present invention can significantly inhibit the expression of myostatin in muscle satellite cells, improve the differentiation ability of muscle satellite cells, and thus promote muscle growth.

The present invention addresses the problem of providing a composition, particularly a pharmaceutical composition and / or a food / beverage composition, for inducing the differentiation of a skeletal muscle and / or promoting the regeneration of a skeletal muscle. More specifically, the present invention addresses the problem of developing a component for promoting the differentiation of a satellite cell or a myoblast into a muscle fiber and promoting the formation of a skeletal muscle. A composition containing fucose or a fucose-releasing compound as an active ingredient has an excellent activity to promote the induction of skeletal muscle differentiation and / or the regeneration of a skeletal muscle, and is therefore useful for various applications in which the formation of a skeletal muscle is desired.

The invention belongs to the technical field of biological materials, and particularly relates to skeletal muscle structure and microenvironment bionic sponge and a preparation method and application thereof. The skeletal muscle microenvironment-like decellularized ECM and a directional freezing technology are utilized to successfully construct the skeletal muscle structure and microenvironment double bionic decellularized ECM / sodium alginate sponge, the physiological skeletal muscle microenvironment can be fully simulated, and the migration, proliferation and differentiation of muscle satellite cells are effectively promoted, so that the regeneration, maturation and differentiation of muscle fibers in the sponge are promoted. Therefore, the invention can effectively solve the dilemma and limitation of fibrosis of the current decellularized ECM in VML treatment application, promote regeneration and repair of damaged tissues of the VML, provide a new treatment approach for regeneration and functional recovery of muscular tissues of the VML, lay a theoretical and technical foundation for research and development of the decellularized ECM with clinical potential application value and a scaffold thereof, and have a wide application prospect. Therefore, the method has important clinical potential significance.

The invention relates to the technical field of fish cell culture, cell differentiation regulation and control and cell culture aquatic product tissue construction, in particular to a method for regulating and controlling fish stem cell co-culture and synchronously inducing myobiogenic and adipogenic differentiation based on microcarrier matrix stiffness. Adding the fish muscle satellite cells, the fish adipose-derived stem cells and the two microcarriers into the same culture system, and performing suspension dynamic culture; the culture system at least comprises two types of microcarriers: a high-rigidity microcarrier and a low-rigidity microcarrier. According to the method, the same culture system is adopted in a single reactor to penetrate through the whole process, mechanical microenvironment difference is provided by simultaneously configuring microcarriers with different rigidities, and the mechanical microenvironment difference is provided under the conditions that a muscle formation / fat formation induction stage is not additionally set and induction factors are not added; muscular satellite cells and adipose-derived stem cells are synchronously subjected to myoblast and adipogenic differentiation in the same system, and product myolipid tissue controllability and batch-to-batch repeatability are achieved through the microcarrier proportion and the cell proportion.

To provide a relatively safe muscle building agent by which muscle mass and muscle force can be effectively increased or maintained and by which a decrease of muscle mass and muscle force can be prevented or suppressed by activating muscle satellite cells.SOLUTION: A muscle building agent according to the present invention comprises a specific ellagitannin as an active ingredient.SELECTED DRAWING: None

The present application belongs to the field of cell culture and biological manufacturing, and particularly relates to a non-animal source surface modified microcarrier with a surface limited adhesion promoting layer, and a preparation method and application thereof. By constructing a main gel network skeleton with agar and gellan gum, and adjusting the flexibility, water absorption behavior and wet integrity of the system through dextran and / or gum arabic, the stability of the adhesion promoting interface is formed on the surface of the microcarrier under the condition of not introducing animal source adhesion factors by means of pre-gel stage regulation, two-stage ion locking, permeation reconstruction of surface microstructure and surface limited self-assembly, so as to improve the long-term culture adaptability of the microcarrier in a dynamic shear environment. The microcarrier can be prepared in spherical, sheet or block morphologies, and is used for adherent cell expansion, secretion product collection and dynamic culture amplification process, and is further suitable for the culture of fish muscle satellite cells, adipose-derived cells, mesenchymal-like cells and other adherent dependent cells.

The application discloses a serum-free culture method for inducing differentiation of muscle satellite cells into myotubes. The inventors have found that the muscle satellite cells can be efficiently induced to differentiate into myotubes with a differentiation rate of up to 46.2% by sequentially adding dexamethasone and insulin into a serum-free basic culture medium. The serum-free culture method has the advantages that no serum or other components with unknown compositions need to be added into the culture medium, the chemical composition of the culture medium is clear, the stability of the induced culture is good, the cost is controllable, and the method is safer.

The application discloses a skeletal muscle repair method and system based on muscle satellite cell regulation, and performs proliferation differentiation correlation analysis on satellite cell steady state data and aerobic exercise intensity data to establish a proliferation regulation map; a synergistic repair time window is determined according to the map, a difference response deviation correction parameter is extracted to form an activity correction factor, repair intensity correction is performed on the synergistic repair time window to generate a self-adaptive repair window; the self-adaptive repair window is subjected to exercise prescription adaptation and satellite cell activation regulation pathways are identified, an activation delay time is obtained through activation efficiency mapping, a response level is generated according to the activation delay time to form a layered repair configuration; a preferred activation mode is determined through activation delay evaluation on the layered repair configuration, dynamic activation characteristics are formed by extracting data of the best activation time window, skeletal muscle repair execution instructions are output in combination with an exercise prescription parameter table, and satellite cell repair state monitoring and dynamic synergistic adaptation of training scheme parameters are realized.

The invention discloses an application of 6-shogaol in preparation of a medicine for promoting skeletal muscle regeneration and / or repair. The 6-shogaol can activate stationary-stage muscle satellite cells, promote myoblast differentiation and myotube fusion, guide damaged broken end new muscle fiber connection and realize structure repair. Meanwhile, 6-shogaol can inhibit excessive deposition of collagen, reduce scar formation and promote functional regeneration. In the fracture accompanied with muscle injury, the bone healing quality is synergistically enhanced by improving the soft tissue environment and vascularization, new bone formation is increased, and the problem of bone nonunion caused by muscle injury is solved. In a word, the 6-shogaol can solve the pain point that the physical injury of the skeletal muscle is difficult to heal through the dual mechanisms of promoting muscle regeneration and resisting fibrosis, and cooperatively promotes the accompanying bone injury repair. The application is different from conventional osteoporosis treatment or disuse atrophy treatment, and has definite clinical pertinence and application value.

To provide a muscle enhancing agent and an oral composition for muscle enhancement that exhibit muscle satellite cell activation effects while ensuring high safety.SOLUTION: A muscle enhancing agent contains a plasmalogen as an active ingredient.SELECTED DRAWING: Figure 4