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30 results about "Muscle satellite cell" patented technology
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Method for isolated culture of human fat mesenchyma stem cell and special culture medium thereof
ActiveCN101314766AThe method of isolation and culture is simpleImprove efficiencySkeletal/connective tissue cellsAntigenMuscle injury
The invention discloses a method for separately culturing a human adipose mesenchymal stem cell and a dedicated culture medium thereof. The culture medium used for separately culturing the human adipose mesenchymal stem cell comprises an animal cell basic culture medium, fetal calf serum, an epidermal growth factor and a platelet-derived growth factor. The final concentration of the fetal calf serum is 1-200 mL / L, the final concentration of the epidermal growth factor is 1-100 ng / ml, and the final concentration of the platelet-derived growth factor is 1-100 ng / ml. The adipose mesenchymal stem cell of the invention has CD31-, CD34-, CD45- and HLA-DR-, as well as the phenotype of CD29+, CD44+, CD105+ and Flk-1+. The specificity cell surface marker and the relevant antihelion molecule of a skeletal muscle cell and a vascular endothelia cell can be expressed after inducement is performed in vitro. Muscle fiber, vascular endothelin and functional muscle satellite cells can be differentiated in a muscle injury model mouse body caused by medicine and the expression of dystrophin protein on the ducheme muscular dystrophy (DMD) model mouse (mdx) myolemma can be partially recovered, so as to release the pathological symptom of the model mouse.
Owner:微能生命科技集团有限公司
Isolated Muscle Satellite Cells, Use Thereof in Muscle Tissue Repair and Method for Isolating Said Muscle Satellite Cells
InactiveUS20070224168A1Small sizeControl granularityBiocideMuscular disorderMuscle tissueMuscle satellite cell
The present invention relates to the field of tissue engineering, and more particularly to isolated muscle satellite cells, their use for repairing damaged muscle tissues and a method for isolating said muscle satellite cells. Consequently, the present invention relates to isolated muscle satellite cells and a method for isolating muscle satellite cells, the use of such satellite cells in composition and method for repairing a damaged muscle tissue of a patient.
Owner:INST PASTEUR +1
Nitric oxide manipulation of muscle satellite cell activation
InactiveUS6967102B1Change effectEnhance the beneficial effectHalogenated hydrocarbon active ingredientsCompound screeningWhole bodyBiological activation
The present invention is directed to methods, pharmaceutical compositions and kits for modulating skeletal muscle precursor cell activation. Modulation is effected through the use of nitric oxide (NO), donors of NO, inhibitors of NO activity (NO inhibitor) or regulators of NO production, either locally or systemically. The invention further teaches the use of NO, an NO donor, an NO inhibitor or a regulator of NO production to modulate the effects of steroid hormone on skeletal muscle. The invention further provides a method for identifying a compound which effects a change in activation state of muscle precursor cells. A number of advantages is evident. By allowing skeletal muscle precursor cells to be manipulated directly, the invention enables specific treatments to regenerate and repair muscle.
Owner:UNIVERSITY OF MANITOBA
Paralichthys olivaceus muscle satellite cell line establishing method, specific primer for identifying paralichthys olivaceus muscle satellite cell marker gene and application of specific primer
PendingCN105200005ACultivate suitableSimple and fast operationMicrobiological testing/measurementVertebrate cellsResuscitationFunctional genes
The invention relates to a marine fish cell culture technology, in particular to an aralichthys olivaceus muscle satellite cell line establishing method, a specific primer for identifying a paralichthys olivaceus muscle satellite cell marker gene and application of the specific primer. The aralichthys olivaceus muscle satellite cell line establishing method comprises the following steps: performing primary culture and subculture of aralichthys olivaceus muscular tissues, and then establishing a cell line; after establishing, performing freezing preservation, resuscitation and identification, wherein a cell culture fluid adopted to primary culture and subculture is obtained through adding fetal calf serum and a human basic fibroblast growth factor into the cell culture fluid. The built aralichthys olivaceus muscle satellite cell line is in the format of fusiform or spindle-shaped monocytes, allows continuous passage, can provide a great number of aralichthys olivaceus muscle satellite cells, and not only can be directly used for researches on aralichthys olivaceus muscle development related functional genes to provide rich cell sources for researches on the molecular mechanism of a fish muscle differentiation pathway, but also is expected to provide a research platform for researches and application of muscle character improvement during aralichthys olivaceus culture production.
Owner:INST OF OCEANOLOGY - CHINESE ACAD OF SCI
Method for facilitating in vitro multiplication of bovine skeletal muscle satellite cell
ActiveCN103710387ATransfection monitoringLittle influence of other biological propertiesVector-based foreign material introductionForeign genetic material cellsSkeletal Muscle Satellite CellsBovine muscle
The invention relates to a method for facilitating in vitro multiplication of a bovine skeletal muscle satellite cell. The method comprises the following steps: obtaining a target sequence containing a bovine miR-133b gene sequence; building an expression vector pEGFP-C1-miR133b; transfecting the bovine skeletal muscle satellite cell by the pEGFP-C1-miR133b. By application of the method for facilitating in vitro multiplication of the bovine skeletal muscle satellite cell, the in vitro multiplication ability of the bovine skeletal muscle satellite cell is improved by obtaining over-expression miniature RNA miR-133b of the bovine skeletal muscle satellite cell and using the effect of the miR-133b, and differentiation is effectively inhibited. The over-expression vector of the miR-133b is built by using the pEGFP-C1, and amplification and differentiation of a bovine muscle satellite cell are regulated by the miniature RNA, so as to obtain a high-purity bovine muscle satellite cell. A good cell model is provided for researching the function and the effect of each regulatory factor in amplification and differentiation processes of the muscle satellite cell.
Owner:TIANJIN AGRICULTURE COLLEGE
Method for separating and purifying high-purity chicken-precursor intramuscular fat cells and establishing intramuscular-fat-cell-and-muscle-satellite-cell co-culturing system
ActiveCN106591224ANo pollution in the processSimple technical operationSkeletal/connective tissue cellsMuscle tissueBottle
The invention relates to a method for separating and purifying high-purity chicken-precursor intramuscular fat cells and establishing an intramuscular-fat-cell-and-muscle-satellite-cell co-culture system. The method includes the steps that chicken breast muscle tissue is cut into meat paste, and the meat paste is digested with I-type collagenase and centrifuged; upper-layer mature intramuscular fat cells are sucked, inoculated into a culture bottle and subjected to dedifferentiation treatment, and the chicken-precursor intramuscular fat cells are finally obtained; lower-layer cells obtained after centrifuging are precipitated and resuspended, the cells are purified in a differential-wall-attachment mode, and high-purity muscle satellite cells are obtained; the precursor intramuscular fat cells and the muscle satellite cells are respectively inoculated into a transwell chamber or a matched culture plate, and the co-culturing system is established. By means of the method, the technical bottleneck that the chicken-precursor intramuscular fat cells cannot be independently separated and purified all the time is broken through, and the method can be applied to quantitatively simulating muscular tissue, accurately researching the interactional relationship and the molecular regulation mechanism of the chicken intramuscular fat cells and muscle cells in vitro, and screening, researching and developing related nutrients or medicine.
Owner:INST OF ANIMAL SCI OF CHINESE ACAD OF AGRI SCI
Composition for suppressing muscular fatty change
InactiveCN107530320AQuality improvementGood qualityOrganic active ingredientsOrganic chemistryGlycoside formationMuscle satellite cell
The present invention addresses the problem of providing: a novel composition for suppressing muscular fatty change, which contains a component that can be ingested safely for a long period; and others. It is found that quercetin or a glycoside thereof can suppress the process of the differentiation of a muscle satellite cell into an adipocyte-like cell. In the present invention, quercetin or a glycoside thereof is used in a composition for suppressing muscular fatty change and the like.
Owner:SUNTORY HLDG LTD
Method for isolated culture of human fat mesenchyma stem cell and special culture medium thereof
ActiveCN101314766BThe method of isolation and culture is simpleImprove efficiencySkeletal/connective tissue cellsAntigenMuscle injury
Owner:微能生命科技集团有限公司
Compositions and methods of modulating hif-2a; to improve muscle generation and repair
ActiveUS20190151347A1Reduces and slows progressionReduces and slows and developmentNervous disorderMicrobiological testing/measurementDiseaseMyopathy
Compositions and methods for modulating HIF-2α to meditate of hypoxia signaling in satellite cells and applications thereof for improving skeletal muscle generation and repair are provided. For example, methods of enhancing, increasing, accelerating or / and otherwise improving skeletal muscle generation or regeneration in a subject in need thereof are disclosed. In some embodiments, the methods include administering the subject an effective amount of HIF-2α inhibitor. The HIF-2α inhibitor can be effective to, for example, increase muscle satellite cell proliferation, differentiation, or a combination thereof in a subject. Composition and methods for improving respiration, and reducing or preventing the development or progression of fibrosis are also provided. The disclosed compositions and methods are particularly useful for treating muscular dystrophies, myopathies, and other muscle-related diseases and disorders.
Owner:UNIV OF GEORGIA RES FOUND INC
Electrical stimulation method as well as relative device and system thereof
InactiveCN101637629AOptimize quantityFunction increaseArtificial respirationElectricityPhysical therapy
The invention provides an electrical stimulation method as well as relative device and system thereof. The system comprises an output module and a stimulation module, wherein the output module obtainslow-frequency and long-term electrical stimulation parameters and transmits the electrical stimulation parameters to the stimulation module; and the stimulation module acts on skeletal muscle according to an electrical stimulation signal produced by the electrical stimulation parameters. With the electrical stimulation method, the system can increase the number and improve the proliferation and the differentiation of muscle satellite cells. A device adopting the system has small size, light weight and convenient carrying and use.
Owner:THE HONG KONG POLYTECHNIC UNIV
Application of ferroptosis inhibitor in preparation of preparation for improving motion function of aged individuals
PendingCN113181159AImprove movement disordersPromote absorptionOrganic active ingredientsMicrobiological testing/measurementIntraperitoneal routeFerroportin
The invention discloses an application of a ferroptosis inhibitor in preparation of a preparation for improving the motion function of aged individuals. Through transcriptome sequencing analysis, Tfr1 expression in skeletal muscle and muscle satellite cells is reduced along with age increase. The specific knockout of the Tfr1 muscle satellite cells leads to irreversible inactivation of the muscle satellite cells. The skeletal muscle regeneration process of the Tfr1 muscle satellite cell specific mouse is accompanied by amyotrophy, iron ion accumulation and increase of unsaturated fatty acid biosynthesis, and then skeletal muscle ferroptosis is induced. The senescent skeletal muscle shows that the expression of Tfr1 is reduced, and the surface of an Slc39a14 membrane is enriched, so that the absorption of non-iron transporter binding iron is promoted to be increased, and the occurrence of ferroptosis is promoted. By intraperitoneal injection of the Ferrostatin-1 into aged mice, muscle aplastic disorder and dyskinesia caused by skeletal muscle ferroptosis can be remarkably improved.
Owner:GUANGDONG INST OF MICROBIOLOGY GUANGDONG DETECTION CENT OF MICROBIOLOGY +1
Isolation and characterization of muscle regenerating cells
Cell populations enriched for human myogenic progenitors are obtained by selection on the basis of expression of specific cell surface markers. The muscle progenitor cells are characterized as being CD45−, Mac-1−, GlycophorinA−, CD31− and CD34−, ITGA7hi and CD56 intermediate and methods of use thereof. Methods are provided for the separation and characterization of human myogenic cells, which are precursor cells having the ability to form muscle. The cells are identified and isolated from cells found within the pool of muscle satellite cells, located beneath the basal lamina of mature muscle fibers in the muscle tissue.
Owner:JOSLIN D ABETES CENTER INC
In-situ hybridization probe primer, probe and mapping method of muscle satellite cell of sebastes schlegeli
ActiveCN111118003AContribute to further study of growth and differentiation rulesMicrobiological testing/measurementDNA/RNA fragmentationMuscle tissueHybridization probe
The invention relates to an in-situ hybridization probe primer, a probe and a mapping method of the muscle satellite cell of sebastes schlegeli and belongs to the field of molecular biology. A probe template has a primer sequence as shown in SEQ. NO 1 and SEQ. NO 2 and has a probe sequence as shown in SEQ. NO 3. The invention further provides a method for performing in-situ hybridization mapping on the muscle satellite cell of sebastes schlegeli by utilizing the probe. According to the method, the muscle satellite cell of sebastes schlegeli is mapped through skeletal muscle tissue in-situ hybridization of a Pax7 gene, theoretical and technical support can be provided to molecular mechanism study for exploring updating and activating of the muscle satellite cell, and further study on a muscle growth differentiation rule of sebastes schlegeli is favored. Furthermore, the method has a certain application prospect in the aspects of gene mapping and functional study on muscle tissue in-situhybridization of other fishes.
Owner:OCEAN UNIV OF CHINA
In situ hybridization probe primers, probes and localization methods of Xu's flat scorpion muscle satellite cells
ActiveCN111118003BContribute to further study of growth and differentiation rulesMicrobiological testing/measurementDNA/RNA fragmentationMuscle tissueHybridization probe
The invention relates to an in situ hybridization probe primer, probe and positioning method of Xu's flat scorpion muscle satellite cells, which belong to the field of molecular biology. The primer sequences of the probe template are as SEQ.NO 1 and SEQ.NO 2, and the probe The sequence is shown in SEQ.NO 3. The invention also provides a method for using the probe to carry out in situ hybridization to localize the satellite cells of the flat scorpionfish muscle. The invention locates Xu's flat scorpion muscle satellite cells through the in situ hybridization of skeletal muscle tissue of Pax7 gene, provides theoretical and technical support for exploring the renewal and activation molecular mechanism of muscle satellite cells, and will help to further study Xu's flat Muscle growth and differentiation rules of scorpionfish; in addition, the invention has a certain application prospect in the research of gene positioning and function by in situ hybridization of muscle tissues of other fishes.
Owner:OCEAN UNIV OF CHINA
Freshwater fish resting state muscle satellite cell stabilizer
ActiveCN105368835AMature production processImprove product qualityAnimal husbandryDNA/RNA fragmentationDouble strandedMuscle satellite cell
The invention discloses a freshwater fish resting state muscle satellite cell stabilizer which is prepared from double-strand small RNA molecules which are marked, chemically modified and chemically synthesized. The forward sequence of the double-strand small RNA molecules is 5'-AAAUGUAGUAGACUAUAAGUAU-3'. The reverse sequence of the double-strand small RNA molecules is 5'-AsUsACUUAUAGUCUACUACAsUsUsUs-Chol3'. The reverse sequence is used for chemical modification. The 3' end is provided with a cholesterol modifier. The 5' end is provided with two sulpho-modifiers, and the 3' end is provided with four sulpho-modifiers. The reverse sequencec is used for full-base methylation modification. A novel small RNA compound keeping the freshwater fish muscle satellite cell resting state is artificially synthesized. The prepared stabilizer can obviously inhibit the content of main protein promoting muscle satellite cell activation, and the resting state of freshwater fish muscle satellite cells is kept.
Owner:HUNAN AGRICULTURAL UNIV
Application of ubiquitin-specific protease 8 in regulation and breeding of Hu mutton
The invention relates to the application of ubiquitin-specific protease 8 (USP8) in regulating the meat quality and breeding of Hu mutton. Experiments have found that USP8 can affect the eating quality of Hu sheep muscle. The higher the expression level of USP8, the better the eating quality of Hu sheep. Therefore, Hu sheep with good meat quality can be selected as breeding sheep by screening the expression level of USP8. The invention clarifies that USP8 has an inhibitory effect on the early differentiation process of muscle satellite cells, provides a basic theoretical basis for the further protection and breeding of Hu sheep breeds, and also provides some references and technical reserves for the future directional selection of new strains .
Owner:ZHEJIANG UNIV
Compositions and methods of modulating HIF-2A to improve muscle generation and repair
ActiveUS10953036B2Promotes self-renewal and stemnessEasy to transplantNervous disorderMicrobiological testing/measurementDiseaseMuscular dystrophy
Compositions and methods for modulating HIF-2α to meditate of hypoxia signaling in satellite cells and applications thereof for improving skeletal muscle generation and repair are provided. For example, methods of enhancing, increasing, accelerating or / and otherwise improving skeletal muscle generation or regeneration in a subject in need thereof are disclosed. In some embodiments, the methods include administering the subject an effective amount of HIF-2α inhibitor. The HIF-2α inhibitor can be effective to, for example, increase muscle satellite cell proliferation, differentiation, or a combination thereof in a subject. Composition and methods for improving respiration, and reducing or preventing the development or progression of fibrosis are also provided. The disclosed compositions and methods are particularly useful for treating muscular dystrophies, myopathies, and other muscle-related diseases and disorders.
Owner:UNIV OF GEORGIA RES FOUND INC
A method for promoting the proliferation of bovine skeletal muscle satellite cells in vitro
ActiveCN103710387BTransfection monitoringLittle influence of other biological propertiesVector-based foreign material introductionForeign genetic material cellsSkeletal Muscle Satellite CellsSkeletal muscle satellite cell proliferation
The invention relates to a method for facilitating in vitro multiplication of a bovine skeletal muscle satellite cell. The method comprises the following steps: obtaining a target sequence containing a bovine miR-133b gene sequence; building an expression vector pEGFP-C1-miR133b; transfecting the bovine skeletal muscle satellite cell by the pEGFP-C1-miR133b. By application of the method for facilitating in vitro multiplication of the bovine skeletal muscle satellite cell, the in vitro multiplication ability of the bovine skeletal muscle satellite cell is improved by obtaining over-expression miniature RNA miR-133b of the bovine skeletal muscle satellite cell and using the effect of the miR-133b, and differentiation is effectively inhibited. The over-expression vector of the miR-133b is built by using the pEGFP-C1, and amplification and differentiation of a bovine muscle satellite cell are regulated by the miniature RNA, so as to obtain a high-purity bovine muscle satellite cell. A good cell model is provided for researching the function and the effect of each regulatory factor in amplification and differentiation processes of the muscle satellite cell.
Owner:TIANJIN AGRICULTURE COLLEGE
Application of grass carp fsrp-3 in culture medium and special medium for grass carp muscle satellite cells
ActiveCN107475182BPromote growthShort training periodCulture processSkeletal/connective tissue cellsBiotechnologyZoology
The invention provides application of grass carp FSRP-3 to a culture medium and further correspondingly provides a special culture medium containing grass carp FSRP-3 and used for grass carp muscle satellite cells. The special culture medium for the grass carp muscle satellite cells, provided by the invention, comprises a basic culture medium and additives, wherein the additives comprise the grass carp FSRP-3, grass carp sourced blood serum extract, sodium pyruvate, glutamic acid and vitamin E. By adopting the special culture medium for the grass carp muscle satellite cells, provided by the invention, the grass carp muscle satellite cells can grow vigorously and a cell culture period is shortened; the special culture medium for the grass carp muscle satellite cells has the characteristics of high efficiency, stability and the like and has a wide market prospect.
Owner:CHANGSHA MERRY BIOTECH
Isolation and purification of high-purity chicken precursor intramuscular adipocytes and method for constructing a co-culture system with muscle satellite cells
ActiveCN106591224BHigh purityNo pollution in the processSkeletal/connective tissue cellsMeat pasteMuscle tissue
The invention relates to a method for separating and purifying high-purity chicken-precursor intramuscular fat cells and establishing an intramuscular-fat-cell-and-muscle-satellite-cell co-culture system. The method includes the steps that chicken breast muscle tissue is cut into meat paste, and the meat paste is digested with I-type collagenase and centrifuged; upper-layer mature intramuscular fat cells are sucked, inoculated into a culture bottle and subjected to dedifferentiation treatment, and the chicken-precursor intramuscular fat cells are finally obtained; lower-layer cells obtained after centrifuging are precipitated and resuspended, the cells are purified in a differential-wall-attachment mode, and high-purity muscle satellite cells are obtained; the precursor intramuscular fat cells and the muscle satellite cells are respectively inoculated into a transwell chamber or a matched culture plate, and the co-culturing system is established. By means of the method, the technical bottleneck that the chicken-precursor intramuscular fat cells cannot be independently separated and purified all the time is broken through, and the method can be applied to quantitatively simulating muscular tissue, accurately researching the interactional relationship and the molecular regulation mechanism of the chicken intramuscular fat cells and muscle cells in vitro, and screening, researching and developing related nutrients or medicine.
Owner:INST OF ANIMAL SCI OF CHINESE ACAD OF AGRI SCI
Muscle building agent
PendingUS20220151987A1Increasing and maintaining mass and forceIncreasing and maintaining muscle and muscle forceOrganic active ingredientsMetabolism disorderMuscle tissueDisease
Methods for building muscle tissue in a subject are described, which comprise administering an ellagitannin compound represented by the following formula (I) to a subject in need thereof:The ellagitannin compound represented by the formula (I) may be casuarinin or stachyurin, and is administered in a therapeutically effective amount. For instance, the ellagitannin compound may be administered in an amount effective for activating muscle satellite cells. These methods increase and / or maintain muscle mass, as well as prevent and / or suppress a decrease of muscle mass and / or a decrease of muscle force. In certain embodiments, the subject is a human, such as an elderly person. In particular these methods are useful for subjects having or at risk of diseases such as sarcopenia, locomotive syndrome and / or frailty. The ellagitannin compound represented by the formula (I) may be administered as a component of a food or drink composition.
Owner:KANEKA CORP
Skin repairing liquid containing human stem cell factors and preparation method thereof
InactiveCN113018247AAvoid affecting activityKeep aliveCosmetic preparationsToilet preparationsBiotechnologyGlycerol
The invention provides skin repair liquid containing human stem cell factors and a preparation method thereof, and relates to the technical field of skin repair. The skin repairing liquid containing human stem cell factors is prepared by weighing the following raw materials in parts by weight according to the following formula: 3 to 6 parts of embryonic stem cells, 1 to 5 parts of muscle satellite cells, 8 to 10 parts of skin epidermal stem cells, 20 to 25 parts of calf blood gel matrix, 11 to 20 parts of herba dendrobii extract, 0.5 to 0.8 part of carbomer, 0.2 to 0.6 part of butanediol, 2 to 6 parts of radix notoginseng extract, 11 to 23 parts of phenoxyethanol, 2 to 4 parts of beewax and 0.5 to 0.7 part of glycerol caprylate. The human stem cells are always kept active, the optimal activity of the stem cells is guaranteed in the using process, the skin repairing effect is better, and the repairing effect is better through a human stem cell repairing base solution when the human stem cells are used for repairing.
Owner:云南贺尔思细胞生物技术有限公司
Compositions and methods of modulating hif-2a to improve muscle generation and repair
PendingUS20210169920A1Promotes self-renewal and stemnessEasy to transplantNervous disorderMicrobiological testing/measurementDiseaseMuscular dystrophy
Compositions and methods for modulating HIF-2α to meditate of hypoxia signaling in satellite cells and applications thereof for improving skeletal muscle generation and repair are provided. For example, methods of enhancing, increasing, accelerating or / and otherwise improving skeletal muscle generation or regeneration in a subject in need thereof are disclosed. In some embodiments, the methods include administering the subject an effective amount of HIF-2α inhibitor. The HIF-2α inhibitor can be effective to, for example, increase muscle satellite cell proliferation, differentiation, or a combination thereof in a subject. Composition and methods for improving respiration, and reducing or preventing the development or progression of fibrosis are also provided. The disclosed compositions and methods are particularly useful for treating muscular dystrophies, myopathies, and other muscle-related diseases and disorders.
Owner:UNIV OF GEORGIA RES FOUND INC
Electrical stimulation method as well as relative device and system thereof
InactiveCN101637629BOptimize quantityFunction increaseArtificial respirationElectricityMuscle satellite cell
Owner:THE HONG KONG POLYTECHNIC UNIV
Application of mir-192 in regulation of proliferation and differentiation of sheep skeletal muscle satellite cells
ActiveCN105441448BIncrease meat productionImprove qualityMicrobiological testing/measurementBiological testingSkeletal Muscle Satellite CellsControl cell
The invention discloses the application of a non-coding small RNA miR-192 in inhibiting the myogenic differentiation of sheep skeletal muscle satellite cells and promoting the proliferation of sheep skeletal muscle satellite cells. The analog of the non-coding small RNA miR-192 is transfected into sheep The overexpression of intracellular miR‑192 in skeletal muscle satellite cells, compared with the cells overexpressing the control nucleotide sequence, the differentiation of sheep skeletal muscle satellite cells was inhibited, but the proliferation of sheep skeletal muscle satellite cells was inhibited. Capabilities are improved. The present invention also provides the application of miR-192 to inhibit the expression of RB1 protein level in sheep skeletal muscle satellite cells. The present invention discovers for the first time that miR-192 has the function of negatively regulating the myogenic differentiation of sheep skeletal muscle satellite cells and promoting the proliferation of sheep skeletal muscle satellite cells.
Owner:INST OF ANIMAL SCI OF CHINESE ACAD OF AGRI SCI
Isolated culture method of muscle satellite cells
InactiveCN112608888AAvoid damageShorten separation timeCell dissociation methodsSkeletal/connective tissue cellsBiotechnologyMuscle tissue
The invention discloses an isolated culture method of muscle satellite cells, which comprises the following steps of cutting off leg skin under aseptic conditions, taking leg muscles, and immersing in a buffer solution, removing fat, bones and tendons, and repeatedly cleaning muscle tissues with a buffer solution, shearing muscle tissues into small blocks under a sterile condition, and digesting with collagenase to separate cells, adding an isopyknic culture medium, blowing, beating, filtering, collecting filtrate, centrifuging, discarding supernate, and resuspending cells by using the culture medium, adding the cell suspension into a culture dish, and culturing in a cell incubator, carrying out cell purification culture by a differential attachment method, and when the cell confluence degree reaches 80%, using trypsin for digestion subculture, and when the cell confluence degree reaches 90% or above, carrying out cell cryopreservation. The chicken muscle satellite cells cultured by the isolated culture method disclosed by the invention are higher in activity, more in quantity, easier to adhere to the wall, stronger in passage and higher in differentiation rate, and the culture method is simple, convenient to operate, time-saving and labor-saving.
Owner:BEIJING YULONG SHENGSHI BIOTECH CO LTD
Isolated culture method for chicken muscle satellite cells
InactiveCN110760475AAvoid damageShorten separation timeCell dissociation methodsCulture processMuscle tissueCell survival rate
The invention belongs to the biotechnology field, and particularly relates to an isolated culture method for chicken muscle satellite cells. The isolated culture method for the chicken muscle satellite cells comprises the following steps of: adopting an SPF (Specific Pathogen Free) chicken, and taking the muscle tissues of the SPF chicken; adding a mixed enzyme solution containing collagenase, type I and pancreatin, wherein a ratio of the collagenase, type I to the pancreatin is 1-2:1; digesting at a temperature of 37 DEG C; and through two-time differential attachment purification for 30 minutes, obtaining a target cell. By use of the method, the defects and the deficiencies in the prior art that a cell survival rate is low, a separation amount is small and storage properties are poor canbe effectively eliminated.
Owner:YANGZHOU UNIV
A kind of pig akirin2 gene promoter and its application
InactiveCN104099332BVerify startup efficiencyVector-based foreign material introductionAnimal husbandryNucleotideOligonucleotide primers
The invention discloses a porcine akirin2 gene promoter and application thereof, the nucleotide sequence of which is shown in SEQ:ID:1. The application of the pig akirin2 gene promoter of the invention in the eukaryotic expression of exogenous genes and transgenic pigs. The present invention determined that the smallest promoter fragment of 200bp has the strongest activity. The invention provides an oligonucleotide primer sequence, a cloning vector, an expression vector, a transformed host, and a method for exogenous protein expression. These provide the basis for further research on the regulation of porcine akirin2 gene in porcine muscle satellite cells and other related cells, and can be used in mammalian cells for foreign protein expression and transgenic research.
Owner:HENAN AGRICULTURAL UNIVERSITY
Application of grass carp FSRP-3 to culture medium and special culture medium for grass carp muscle satellite cells
ActiveCN107475182APromote growthShort training periodCulture processSkeletal/connective tissue cellsSodium pyruvateMicrobiology
The invention provides application of grass carp FSRP-3 to a culture medium and further correspondingly provides a special culture medium containing grass carp FSRP-3 and used for grass carp muscle satellite cells. The special culture medium for the grass carp muscle satellite cells, provided by the invention, comprises a basic culture medium and additives, wherein the additives comprise the grass carp FSRP-3, grass carp sourced blood serum extract, sodium pyruvate, glutamic acid and vitamin E. By adopting the special culture medium for the grass carp muscle satellite cells, provided by the invention, the grass carp muscle satellite cells can grow vigorously and a cell culture period is shortened; the special culture medium for the grass carp muscle satellite cells has the characteristics of high efficiency, stability and the like and has a wide market prospect.
Owner:CHANGSHA MERRY BIOTECH
A method for establishing a satellite cell line of flounder embryonic muscle
ActiveCN108753703BOvercome dysfunctional issuesCultivate suitableCell dissociation methodsCulture processEmbryonic StageEmbryonic muscle
The invention relates to a seawater fish embryonic cell culture technology, in particular to a method for establishing a muscle satellite cell line in the embryonic stage of flounder. The embryos of the flounder bursa formation stage were separated in vitro, the egg membrane was removed, the cells were dispersed, and the embryonic muscle satellite cell line was established after primary culture and subculture. The morphology of the flounder embryo muscle satellite cell line established in the present invention is spindle-shaped or spindle-shaped mononuclear cells. In a single generation, if it is cultured for more than 5 days or induced by exogenous factors (horse serum), it can differentiate and form muscle fibers. The cell line can provide a large number of muscle satellite cells, which can be normally transfected or infected by GFP transfection and semi-smooth tongue sole spleen-kidney necrosis virus challenge experiments. Therefore, it can not only be directly used in the research of functional genes related to fish muscle growth and development, provide research materials for exploring the molecular mechanism of induction, proliferation and differentiation of muscle cells, but also provide research materials for the improvement and application of muscle traits in flounder breeding. platform.
Owner:INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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