Application of ubiquitin-specific protease 8 in regulation and breeding of Hu mutton

A specific, protease technology, applied in the application of ubiquitin-specific protease 8 in the regulation of Hu mutton meat quality and breeding, can solve the problem of no USP8 regulating muscle related reports and other issues

Active Publication Date: 2021-05-14
ZHEJIANG UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] Deubiquitinating enzymes have been a research hotspot in recent years, and there have been no reports about USP8 regulating myogenesis

Method used

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  • Application of ubiquitin-specific protease 8 in regulation and breeding of Hu mutton
  • Application of ubiquitin-specific protease 8 in regulation and breeding of Hu mutton
  • Application of ubiquitin-specific protease 8 in regulation and breeding of Hu mutton

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] 1 Test materials and methods

[0037] 1 Test materials and equipment

[0038] 1.1 Test material

[0039] The six-month-old male Hu sheep (Ovis aries) used in this experiment came from Zhongcheng Hu Sheep Professional Cooperative in Tongxiang, Jiaxing City, Zhejiang Province. The average weight of the Hu sheep was 48.50±2.95kg. Hu sheep were fasted 24 hours before sampling. The F1 generation of six-month-old male Duhu hybrid sheep used in this experiment came from Lin'an sheep farm in Hangzhou City, Zhejiang Province, and also fasted for 24 hours before sampling.

[0040] The Huyang muscle satellite cells used in this experiment were donated by the research group of Professor Sun Wei of Yangzhou University.

[0041] 1.2 Main chemical reagents

[0042] Absolute ethanol (Shanghai Hushi, Sinopharm Chemical Reagent Co., Ltd.); DEPC treated water, 1×PBS buffer solution (Shanghai Sangon Biotechnology Co., Ltd.); xylene (Sinopharm Chemical Reagent Co., Ltd.); masson dye sol...

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Abstract

The invention relates to the application of ubiquitin-specific protease 8 (USP8) in regulating the meat quality and breeding of Hu mutton. Experiments have found that USP8 can affect the eating quality of Hu sheep muscle. The higher the expression level of USP8, the better the eating quality of Hu sheep. Therefore, Hu sheep with good meat quality can be selected as breeding sheep by screening the expression level of USP8. The invention clarifies that USP8 has an inhibitory effect on the early differentiation process of muscle satellite cells, provides a basic theoretical basis for the further protection and breeding of Hu sheep breeds, and also provides some references and technical reserves for the future directional selection of new strains .

Description

[0001] (1) Technical field [0002] The invention belongs to the technical field of animal molecular markers, and in particular relates to the application of ubiquitin-specific protease 8 in regulating the meat quality and breeding of Hu mutton. [0003] (2) Background technology [0004] Hu sheep belongs to Mammalia, Artiodactyl, Bovine, and Sheep. It is a first-class local livestock and poultry protected species in my country, and it is also one of the world-renowned multi-parity sheep breeds. Hu mutton is rich in taste, tender and juicy, and has the characteristics of early maturity and strong fecundity, and has extremely high economic value. [0005] Hu sheep have strong adaptability to the environment, early sexual maturity, suitable for captivity, and strong fecundity, and are suitable as the female parent of hybrid breeding. However, the problems of low carcass lean meat rate and slow individual growth and development cannot be ignored. Exploring the genes related to th...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6888
CPCC12Q1/6888C12Q2600/124C12Q2600/158
Inventor 王争光邱寒峰褚玲娜王邵雨
Owner ZHEJIANG UNIV
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