Method for isolated culture of human fat mesenchyma stem cell and special culture medium thereof

Abstract

The invention discloses a method for separately culturing a human adipose mesenchymal stem cell and a dedicated culture medium thereof. The culture medium used for separately culturing the human adipose mesenchymal stem cell comprises an animal cell basic culture medium, fetal calf serum, an epidermal growth factor and a platelet-derived growth factor. The final concentration of the fetal calf serum is 1-200 mL / L, the final concentration of the epidermal growth factor is 1-100 ng / ml, and the final concentration of the platelet-derived growth factor is 1-100 ng / ml. The adipose mesenchymal stem cell of the invention has CD31-, CD34-, CD45- and HLA-DR-, as well as the phenotype of CD29+, CD44+, CD105+ and Flk-1+. The specificity cell surface marker and the relevant antihelion molecule of a skeletal muscle cell and a vascular endothelia cell can be expressed after inducement is performed in vitro. Muscle fiber, vascular endothelin and functional muscle satellite cells can be differentiated in a muscle injury model mouse body caused by medicine and the expression of dystrophin protein on the ducheme muscular dystrophy (DMD) model mouse (mdx) myolemma can be partially recovered, so as to release the pathological symptom of the model mouse.

Description

technical field

[0001] The invention relates to a method for separating and culturing human adipose mesenchymal stem cells and a special culture medium thereof. Background technique

[0002] Stem cells are primitive cells with self-renewal ability and multi-lineage differentiation potential, and are ideal cells for cell transplantation. During individual development, there are various forms of stem cells, such as embryonic stem cells, pluripotent stem cells, and committed stem cells in various tissues such as hematopoietic stem cells, neural stem cells, etc. However, in practical application, it faces ethical challenges or limitations of material selection. Mesenchymal stem cells (MSC) are a subpluripotent stem cell population with multi-lineage differentiation potential in human tissues, because they can be induced to differentiate into a variety of tissue cells in a specific environment, and have low immunogenicity. It can form stable chimerism and other characteristics,...

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