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Application of grass carp fsrp-3 in culture medium and special medium for grass carp muscle satellite cells

A FSRP-3, muscle satellite cell technology, applied in the culture process, animal cells, tissue culture and other directions, can solve the problems of grass carp muscle satellite cell growth rate, cell density and cell activity that do not meet the experimental requirements, and achieve the grass carp muscle fiber cell nucleus. The positive rate of and muscle satellite cells is increased, the growth of grass carp muscle satellite cells is promoted, and the economic benefits are significant.

Active Publication Date: 2020-10-09
CHANGSHA MERRY BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the wide variety of fish, each fish muscle tissue has its own characteristics. It has been reported that most of the culture medium is suitable for seawater fish, and there is no report on the relevant medium of freshwater fish, especially grass carp. Satellite cell growth rate, cell density and cell activity did not meet the experimental requirements

Method used

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  • Application of grass carp fsrp-3 in culture medium and special medium for grass carp muscle satellite cells
  • Application of grass carp fsrp-3 in culture medium and special medium for grass carp muscle satellite cells
  • Application of grass carp fsrp-3 in culture medium and special medium for grass carp muscle satellite cells

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Embodiment 1

[0024] A special culture medium for grass carp muscle satellite cells of the present invention, comprising basal culture medium and supplements, the content of supplements is: grass carp FSRP-3 0.2-0.8 mg / L, grass carp source serum extract 1-5mg / L, sodium pyruvate 5-8 mg / L, glutamic acid 2-5mg / L and vitamin E 5-10mg / L, among them, grass carp FSRP-3 was obtained by the following method: collecting grass carp early embryos, After the embryos were broken, they were centrifuged at 1500r / min for 10min, and after standing still, the supernatant was passed through a chromatography column containing grass carp FSRP-3 monoclonal antibody to obtain purified grass carp FSRP-3. The special medium for grass carp muscle satellite cells of this example is used for the cultivation of grass carp muscle satellite cells.

Embodiment 2

[0026] A special culture medium for grass carp muscle satellite cells of the present invention, comprising basal culture medium and supplements, the content of the supplements is: Grass carp FSRP-3 0.2-0.7 mg / L, Grass carp source serum extract Substance 1-3mg / L, sodium pyruvate 5-5.5 mg / L, glutamic acid 2-2.5mg / L and vitamin E 5-6mg / L. Among them, grass carp FSRP-3 is obtained by the following method: collect early grass carp embryos, break the embryos and centrifuge at 1500r / min for 10min, after standing still, take the supernatant and pass through the column containing grass carp FSRP-3 monoclonal antibody to obtain purified grass carp FSRP -3. The special medium for grass carp muscle satellite cells of this example is used for the cultivation of grass carp muscle satellite cells.

Embodiment 3

[0028] A special culture medium for grass carp muscle satellite cells of the present invention, comprising basal culture medium and supplements, the content of the supplements is: Grass carp FSRP-3 0.2-0.9 mg / L, Grass carp source serum extraction Substance 1-2.5mg / L, sodium pyruvate 5-5.5 mg / L, glutamic acid 2-2.5mg / L and vitamin E 5-6mg / L. Among them, grass carp FSRP-3 is obtained by the following method: collect early grass carp embryos, break the embryos and centrifuge at 1500r / min for 10min, after standing still, take the supernatant and pass through the column containing grass carp FSRP-3 monoclonal antibody to obtain purified grass carp FSRP -3. The special medium for grass carp muscle satellite cells of this example is used for the cultivation of grass carp muscle satellite cells.

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Abstract

The invention provides application of grass carp FSRP-3 to a culture medium and further correspondingly provides a special culture medium containing grass carp FSRP-3 and used for grass carp muscle satellite cells. The special culture medium for the grass carp muscle satellite cells, provided by the invention, comprises a basic culture medium and additives, wherein the additives comprise the grass carp FSRP-3, grass carp sourced blood serum extract, sodium pyruvate, glutamic acid and vitamin E. By adopting the special culture medium for the grass carp muscle satellite cells, provided by the invention, the grass carp muscle satellite cells can grow vigorously and a cell culture period is shortened; the special culture medium for the grass carp muscle satellite cells has the characteristics of high efficiency, stability and the like and has a wide market prospect.

Description

technical field [0001] The invention belongs to the technical field of animal cell culture, and in particular relates to a special culture medium for grass carp muscle satellite cells. Background technique [0002] Muscle is a reservoir of adult stem cells with multilineage differentiation potential. There are at least two types of stem cells in muscle: muscle satellite cells (MSC) and muscle derived stern cells (MDSC). Since muscle satellite cells account for the vast majority, muscle stem cells mainly refer to muscle satellite cells. Muscle satellite cells are only present in skeletal muscle, but there are no muscle satellite cells in cardiac muscle and smooth muscle. The number of muscle fibers of higher vertebrates will not increase after birth, and the growth and thickening of muscle fibers are mainly used to maintain body growth. Muscle satellite cells are activated, differentiated and self-renewed only when muscles are stimulated by factors such as damage, but post-e...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/077
CPCC12N5/0659C12N2500/30C12N2500/32C12N2500/38C12N2501/998
Inventor 褚武英成嘉李玉珑吴萍
Owner CHANGSHA MERRY BIOTECH
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