Methods for
in vitro isolation and culture and induced differentiation of
bovine muscle satellite cells relate to the methods for
in vitro isolation and culture and induced differentiation of cells. The invention solves the following problems: the cost is high and the
heredity stability is not good in the existing method for isolation and culture of
bovine muscle satellite cells, and the obtained myotubes are fewer and do not have contracting function in the existing method for induced differentiation of
bovine muscle satellite cells. The method for
in vitro isolation and culture of bovine
muscle satellite cells is characterized by cleaning the tissues and digesting the tissues with
trypsin and
collagenase solution; carrying out centrifuging after filtering, re-suspending the cells and culturing the cells by a differential velocity adherent method after
inoculation; and culturing the cells until the merging rate is 95% and carrying out
heredity with pancreatin. The method for induced differentiation of bovine
muscle satellite cells is characterized by obtaining PEI-
plasmid complexes; culturing the bovine
muscle satellite cells until the merging rate is 75%, adding high-glucose DMEM culture solution after cleaning, adding the PEI-
plasmid complexes to the surfaces of the cells, changing culture solution A for culturing and then changing culture solution B for induced differentiation. In the method, the cost for isolation and culture of the bovine muscle satellite cells is low, the
heredity stability is good and the myotubes obtained through induced differentiation are large in quantity and have contracting function.