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Method for increasing availability of receptor cattle in bovine embryo transplantation

A cattle embryo and availability technology, applied in the field of animal husbandry, can solve the problems of delaying the estrus period and prolonging the estrus cycle, and achieve the effect of improving the estrus in the same period

Active Publication Date: 2020-08-25
天津力牧生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The effect of using progesterone progesterone and its analogues is to maintain a certain level of progesterone in the blood through continuous use, inhibiting the growth and development of follicles on the ovary and estrus, always in the artificial luteal phase, and sometimes the corpus luteum on the ovary has disappeared , which also means that the level of endogenous hormones has decreased, but because the exogenous hormones are still functioning, the cows will not be in estrus, which is equivalent to prolonging the estrus cycle and delaying the estrus

Method used

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  • Method for increasing availability of receptor cattle in bovine embryo transplantation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0066] Embodiment 1: Synchronous estrus effect test

[0067] Carry out the estrus synchronous effect test according to the following steps.

[0068] (1) Donor cows: Nanyang Yellow Cattle, a number of multiparous cows that are more than 4 months postpartum, aged 3 to 8 years, empty pregnant, and have not been in estrus, and have no reproductive tract diseases confirmed by rectal examination; throughout the test period Mainly grazing during the day, supplementing forage at night;

[0069] (2) Administration: intramuscular injection of luteinizing hormone-releasing hormone A3 (LHRH-A3) to the donor cattle, the injection dose is 5 μg / 100kg body weight;

[0070] (3) Estrus and insemination: counting from 0 day on the day of drug injection, continuously observe the estrus and ovulation of cows within 1 to 3 days, record the time when cows start to estrus and the time when estrus ends, observe once a day in the morning and evening, each time 2 to 3 hours, and the estrus symptoms o...

Embodiment 2

[0076] Embodiment 2: Synchronous estrus effect test

[0077] Carry out the estrus synchronous effect test according to the following steps.

[0078] (1) Donor cows: Nanyang Yellow Cattle, a number of multiparous cows that are more than 4 months postpartum, aged 3 to 8 years, empty pregnant, and have not been in estrus, and have no reproductive tract diseases confirmed by rectal examination; throughout the test period Mainly grazing during the day, supplementing forage at night;

[0079] (2) Administration: intramuscularly inject a composition comprising luteinizing hormone releasing hormone A3 (LHRH-A3) into the donor cattle, and the injection dose is 5 μg / 100kg body weight in terms of LHRH-A3;

[0080] (3) Estrus and insemination: counting from 0 day on the day of drug injection, continuously observe the estrus and ovulation of cows within 1 to 3 days, record the time when cows start to estrus and the time when estrus ends, observe once a day in the morning and evening, each...

Embodiment 3

[0083] Embodiment 3: Synchronous estrus effect test

[0084] Carry out the estrus synchronous effect test according to the following steps.

[0085] (1) Donor cows: Nanyang Yellow Cattle, a number of multiparous cows that are more than 4 months postpartum, aged 3 to 8 years, empty pregnant, and have not been in estrus, and have no reproductive tract diseases confirmed by rectal examination; throughout the test period Mainly grazing during the day, supplementing forage at night;

[0086] (2) Administration: intramuscularly inject a composition comprising luteinizing hormone releasing hormone A3 (LHRH-A3) into the donor cattle, and the injection dose is 5 μg / 100kg body weight in terms of LHRH-A3;

[0087] (3) Estrus and insemination: counting from 0 day on the day of drug injection, continuously observe the estrus and ovulation of cows within 1 to 3 days, record the time when cows start to estrus and the time when estrus ends, observe once a day in the morning and evening, ea...

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Abstract

The invention discloses a method for increasing the availability of receptor cattle in bovine embryo transplantation. By injecting luteinizing hormone-releasing hormone A3 into cows, the technical effect of one or more aspects of the invention can be obtained. The method comprises the following steps: (1) providing a donor cattle; (2) intramuscularly injecting luteinizing hormone-releasing hormoneA3 into the donor cattle; (3) continuously observing oestrus and ovulation conditions of the cow within 3 days from medicine injection, recording oestrus beginning time and oestrus ending time of thecow, observing the oestrus beginning time and the oestrus ending time once every morning and evening, determining oestrus time, oestrus procedures and ovulation conditions by combining rectal examination according to the oestrus symptoms of the cow, judging estrus within 3 days after medicine injection as estrus synchronization, and performing artificial insemination on the estrus ovulated cows at proper time according to operating rules of a freezing technology, with semen being frozen semen of the cows of the same donor; and (4) determining whether the cow is pregnant or not according to arectal examination result 90d after hybridization, and feeding the cow during pregnancy according to a conventional method.

Description

technical field [0001] The invention belongs to the field of animal husbandry and relates to a cattle breeding technology, in particular to a method for increasing the availability of recipient cattle in bovine embryo transplantation. In the present invention, the technical effects of one or more aspects of the present invention can be obtained by injecting luteinizing hormone releasing hormone A3, namely LHRH-A3, into cows. Background technique [0002] At present, when selecting recipient cattle in production, the recipient cattle have two options: natural estrus or injection of drugs to cause simultaneous estrus, and the latter option plays a very important role in improving production efficiency. [0003] Estrus synchronization refers to the use of certain hormone preparations to artificially control and adjust the process of the estrus cycle of a group of female animals, so that they can concentrate on estrus within a predetermined time. Agents that suppress estrus, su...

Claims

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Application Information

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IPC IPC(8): A61D19/04A01K67/02A61K9/00A61K9/19A61K38/24A61K47/14A61K47/18A61P15/08
CPCA61D19/04A01K67/02A61K38/24A61K9/19A61K9/0019A61K47/183A61K47/14A61P15/08
Inventor 郝少强王小武赵明礼王娜郭春明许晓椿
Owner 天津力牧生物科技有限公司
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