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Method for enhancing estrus synchronization effects of cattle embryo transfer and used drugs

A technology of bovine embryos and drugs, applied in the field of animal husbandry, can solve problems such as prolonging the estrus cycle and delaying the estrus period

Active Publication Date: 2020-08-28
天津力牧生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The effect of using progesterone progesterone and its analogues is to maintain a certain level of progesterone in the blood through continuous use, inhibiting the growth and development of follicles on the ovary and estrus, always in the artificial luteal phase, and sometimes the corpus luteum on the ovary has disappeared , which also means that the level of endogenous hormones has decreased, but because the exogenous hormones are still functioning, the cows will not be in estrus, which is equivalent to prolonging the estrus cycle and delaying the estrus

Method used

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  • Method for enhancing estrus synchronization effects of cattle embryo transfer and used drugs

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0085] Embodiment 1: Synchronous estrus effect test

[0086] Carry out the estrus synchronous effect test according to the following steps.

[0087] (1) Donor cows: Nanyang Yellow Cattle, a number of multiparous cows that are more than 4 months postpartum, aged 3 to 8 years, empty pregnant, and have not been in estrus, and have no reproductive tract diseases confirmed by rectal examination; throughout the test period Mainly grazing during the day, supplementing forage at night;

[0088] (2) Administration: intramuscular injection of luteinizing hormone-releasing hormone A3 (LHRH-A3) to the donor cattle, the injection dose is 5 μg / 100kg body weight;

[0089] (3) Estrus and insemination: counting from 0 day on the day of drug injection, continuously observe the estrus and ovulation of cows within 1 to 3 days, record the time when cows start to estrus and the time when estrus ends, observe once a day in the morning and evening, each time 2 to 3 hours, and the estrus symptoms o...

Embodiment 2

[0095] Embodiment 2: Synchronous estrus effect test

[0096] Carry out the estrus synchronous effect test according to the following steps.

[0097] (1) Donor cows: Nanyang Yellow Cattle, a number of multiparous cows that are more than 4 months postpartum, aged 3 to 8 years, empty pregnant, and have not been in estrus, and have no reproductive tract diseases confirmed by rectal examination; throughout the test period Mainly grazing during the day, supplementing forage at night;

[0098] (2) Administration: intramuscularly inject a composition comprising luteinizing hormone releasing hormone A3 (LHRH-A3) into the donor cattle, and the injection dose is 5 μg / 100kg body weight in terms of LHRH-A3;

[0099] (3) Estrus and insemination: counting from 0 day on the day of drug injection, continuously observe the estrus and ovulation of cows within 1 to 3 days, record the time when cows start to estrus and the time when estrus ends, observe once a day in the morning and evening, ea...

Embodiment 3

[0102] Embodiment 3: Synchronous estrus effect test

[0103] Carry out the estrus synchronous effect test according to the following steps.

[0104] (1) Donor cows: Nanyang Yellow Cattle, a number of multiparous cows that are more than 4 months postpartum, aged 3 to 8 years, empty pregnant, and have not been in estrus, and have no reproductive tract diseases confirmed by rectal examination; throughout the test period Mainly grazing during the day, supplementing forage at night;

[0105] (2) Administration: intramuscularly inject a composition comprising luteinizing hormone releasing hormone A3 (LHRH-A3) into the donor cattle, and the injection dose is 5 μg / 100kg body weight in terms of LHRH-A3;

[0106] (3) Estrus and insemination: counting from 0 day on the day of drug injection, continuously observe the estrus and ovulation of cows within 1 to 3 days, record the time when cows start to estrus and the time when estrus ends, observe once a day in the morning and evening, ea...

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Abstract

The invention discloses a method for enhancing estrus synchronization effects of cattle embryo transfer and used drugs, and specifically provides applications of luteinizing hormone-releasing hormoneA3 to preparation of drug compositions for enhancing the estrus synchronization effects of cattle embryo transfer. The method includes the following steps: providing donor cattle; injecting the drugsto the muscle of the donor cattle; continuously observing the estrus and ovulation conditions of cows in 3 days from the day that the drugs are injected, recording the beginning and ending time of estrus of the cows, performing observing in every morning and evening, determining estrus time, estrus procedure and ovulation status by combining the appearance estrus symptoms of the cows with rectal examination, determining estrus synchronization if the cows show estrus in 3 days after the drugs are injected, and timely performing artificial insemination on the cows ovulating in heat according totechnical operating instructions of artificial insemination of frozen semen, wherein semen is frozen semen from the same donor cattle; and after 90 d of mating, determining whether the cows are pregnant by taking rectal examination results as bases, and performing feeding during pregnancy according to conventional methods.

Description

technical field [0001] The invention belongs to the field of animal husbandry and relates to a cattle breeding technology, in particular to a method for improving the effect of bovine embryo transfer estrus synchronization. In the present invention, the technical effects of one or more aspects of the present invention can be obtained by injecting luteinizing hormone releasing hormone A3, namely LHRH-A3, into cows. The present invention also relates to the medicament used in the method for improving the effect of bovine embryo transplantation estrus synchronization. The results of the present invention show that the medicament of the present invention has a significant effect on inducing synchronous estrus for cows that have not been in estrus for a long time or are slow to estrus after giving birth, and is an ideal aphrodisiac drug. Background technique [0002] At present, when selecting recipient cattle in production, the recipient cattle have two options: natural estrus ...

Claims

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Application Information

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IPC IPC(8): A61K38/09A61K31/198A61K31/22A61K9/19A61P15/08A61D19/04
CPCA61K38/09A61K31/198A61K31/22A61K9/0019A61K9/19A61P15/08A61D19/04A61K2300/00
Inventor 郝少强王小武赵明礼王娜郭春明许晓椿
Owner 天津力牧生物科技有限公司
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