50 results about "Estrus synchronization" patented technology

Embodiments of the present invention generally relate to devices and processes related to estrus synchronization. Particular embodiments of devices and processes of the present invention slowly release progesterone over a period of time for estrus synchronization.

Embodiments of the present invention generally relate to devices and processes related to estrus synchronization. Particular embodiments of devices and processes of the present invention slowly release progesterone over a period of time for estrus synchronization.

The invention discloses an extraction method of a micromolecular sheep placenta extract and application of the micromolecular sheep placenta extract in the field of skin-care products, belonging to the technical field of biology. The extraction method disclosed by the invention comprises the following steps of obtaining a lot of sheep pregnant in the same period by adopting synchronization of estrus, taking out sheep fetus after 85-100 days through an operation method to obtain body homogenate, carrying out repetitive freeze-thawing and splitting cells, degenerating at high temperature, sterilizing, precipitating hybrid protein, carrying out 30 KD ultrafiltration after centrifuging at high speed so as to remove toxic and harmful components and impurities, such as macromolecular hybrid protein, bacteria and endogenous virus, so as to obtain a pure micromolecular sheep placenta extract product, and carrying out ultrafiltration and concentration of the micromolecular sheep placenta extract product by using a 1 KD ultrafiltration component. The micromolecular sheep placenta extract disclosed by the invention can be widely used in the fields of skin-care products, beauty products, wound healing products, health-care products, scientific searches and the like; the extraction method disclosed by the invention provides a novel important way for producing the sheep placenta extract; furthermore, the compositions of the micromolecular sheep placenta extract are authenticated for the first time, and thus, theoretical foundation is provided for scientifically using the sheep placenta extract.

The invention discloses a sheep breeding method. The method comprises the steps of selection of a breeding sheep, preconception management, estrus synchronization treatment, artificial impregnation, conception management, preparation before delivery and delivery. In the breeding process of sheep, preconception culture is taken into consideration, concentrated feeds serve as main feeds, rough feeds serve as auxiliary feeds, various conditions of the body of the breeding sheep are enhanced, and the physical quality of a sheep baby after birth is guaranteed; meanwhile, the delivery is expedited by medicines, the conception consistency of the sheep is guaranteed, a pregnant sheep is convenient to manage, and the management difficulty and the management cost are reduced.

The invention discloses a progesterone composite preparation for regulating and controlling estrus of a cow. The progesterone composite preparation is prepared by the following steps of: dissolving progesterone with edible vegetable oil to prepare solution, every milliliter of which contains 20mg of progesterone; dissolving estradiol with sterilized edible vegetable oil to prepare the solution, every milliliter of which contains 10mg of estradiol; and preparing the progesterone composite preparation from 75.00 to 87.50 weight percent of the solution of the progesterone, 8.75 to 10.00 weight percent of the solution of the estradiol and 2.50 to 6.25 weight percent of the edible vegetable oil. The progesterone composite preparation comprises estrogen and progesterone, is injected for regulating and controlling the estrus of the cow, and can obviously improve the estrus synchronization rate and the estrus conception rate of the cow. When used for pre-processing, the progesterone composite preparation can improve the activity of the ovary of the processed cow and the sensitivity to gonadotropic hormone so as to stimulate estrogen secretion and promote follicular development and can prevent insufficient secretion of the progesterone of the processed cow so as not to affect the sexual desire and the estrus of the cow.

The invention discloses a lowline embryo freezing liquid, a freezing method and an unfreezing method and belongs to the technical field of bioengineering and embryonic heredity. The lowline embryo freezing liqui comprises ethanediol, cane sugar and DMSO. The freezing method comprises the following steps: freezing an obtained embryo for pre-treating, putting the embryo in a tube and freezing the embryo. The unfreezing method comprises the following steps: retaining a straw in air for 5-10 seconds, putting the straw in water bath at the temperature of 35-38 DEG C for 10-15 seconds, detecting the embryo, transferring the embryo in WM1 liquid drops, transferring into another hole liquid drops containing the same solution, then respectively transferring to WM2 and liquid drop containing HM with the concentration being 800 microliter per hole and retaining for 5 minutes each time; and washing the embryo by a culture solution for three times, continuously observing the development condition of the embryo in the culture solution or transplanting the embryo to a receptor cow after being subjected to estrus synchronization. The freezing liquid, the freezing method and the unfreezing method have the good freezing effect, are strong in operability, convenient to operate and suitable for popularization and application.

An injectable preparation of estrogenic and progestrogenic hormonal compounds in an anhydrous excipient injected to synchronize estrus in non-menstruating placental female animals, and a five to nine day protocol to apply the unique preparation to achieve estrus synchronization.

The invention relates to a sow raising and breeding method. Through the method, a disperse, random and traditional raising method is converted into concentrated, continuous and balanced assembly line production according to ecological and physiological characteristics of sows, an all-in and all-out process is implemented, and annual balanced production is performed according to the breeding rhythm. The key technology of all-in and all-out production according to the breeding rhythm is estrus synchronization. Estrus synchronization of the sows can be achieved through three methods, namely (1) estrus synchronization of the multiparous sows is achieved through a simultaneous weaning method; (2) estrus synchronization of the sows experiencing the puberty or pregnant sows is achieved through a pen breeding and hormone injection method; (3) estrus synchronization of gilts which are not in the puberty is achieved through a group raising and hormone injection method. The sow raising and breeding method is experience obtained through many years of sow raising, enables the sows to bred regularly, facilitates production and operation management, and enables work to be conducted in an orderly and planned mode.

The invention provides a method for inducing estrus synchronization of replacement gilts, and belongs to the field of animal estrus induction and artificial insemination. The method for inducing estrus synchronization of the replacement gilts is provided, aiming at the problem that estrus of the replacement gilts cannot be synchronous. The replacement gilts are feed with altrenogest within the given time, and combined with intravenous injection of eCG and hCG in the fixed time, so that the replacement gilts can be oestrous synchronously in the preset time. The method is beneficial for large-scale production of gilts.

The invention relates to a method of estrus synchronization of embryo transplantation receptor cow, which belongs to the field of embryo engineering. The method of estrus synchronization of embryo transplantation receptor cow is characterized in that: Cue-Mate (vaginal suppository) is put into the body of the receptor cow on any day of estrus circle of the cow, then the cow is injected with 100microgram of GnRH and the day is set as a first day; on a fifth day to a seventh day, the vaginal suppository is taken out and the cow is injected with 500microgram of PG; two days later, the cow ovulates; and the transplantation is carried out 7 to 7.5 days after the ovulation. Using the method, time for treating the cow is shortened; application efficiency of the cow is enhanced and breeding cost is saved; the ovulation rate of the cow after the treating is enhanced to 85 to 90 percent from the original 75 to 80 percent.

The invention relates to the field of livestock preparations, in particular to a cattle and sheep estrus and pregnancy promoting preparation. The cattle and sheep estrus and pregnancy promoting preparation is prepared from the following raw materials of: 90g of herba epimedii; 60g of dodder; 80g of rhizoma cyperi stir-fried with vinegar; 80g of motherwort herb; 100g of angelica sinensis; 50g of fructus psoraleae; 50g of boschniakia rossica; 40g of prepared rhizome of rehmannia; 20g of Chinese wolfberry; 20g of liquorice. According to the cattle and sheep estrus and pregnancy promoting preparation, a pure traditional Chinese medicine formula is adopted, the cattle and sheep estrus and pregnancy promoting preparation has no toxic or side effect, can realize synchronous estrus of cattle and sheep in a farm, changes the prior breeding mode, fully utilizes animal seed resources to maximize resource utilization, and has good social benefits.

The invention discloses a synchronous estrus and artificial insemination method for inducing batch production of Hu sheep, and belongs to the technical field of efficient breeding of sheep. Accordingto the method provided by the invention, a hormone combination of FGA + PGF2 alpha + PMSG is used for inducing synchronous estrus of the Hu sheep, the vaginal plug burying time is set to be 11 days, and the hormone injection time is 2 days before vaginal plug removal. By adopting the method, the vaginal plug loss rate and the vaginal inflammation rate of ewe can be reduced, ovarian cyst is reduced, meanwhile, the semen deposition frequency and the semen deposition time point are determined, the operability is high, the cost can be saved, and the breeding benefit is improved.

The invention provides a treatment method for anestrus sows and endometritis after applying a timed artificial insemination technology. The treatment method comprises the following steps: (1) synchronizing the sexual cycle of the sows; (2) promoting follicular development of the sows and inducing oestrus synchronization; (3) promoting ovulation synchronization of the sows; (4) treating the anestrus sows: injecting estradiol benzoate into muscles of non-anestrus or anestrus sows; (5) performing timed artificial insemination: after medicaments for promoting folliclematurity and ovulation are applied, performing timed artificial insemination on the non-anestrus sows or the sows which are not obvious in oestrus; (6) treating endometritis: simultaneously applying antibiotic and estradiol benzoate to the mattery sows subjected to hybridization, and then applying cloprostenol sodium. According to the treatment method for the anestrus sows and the endometritis after applying the timed artificial insemination technology, sow resources are fully used, the mortality of multiparous sows is effectively reduced, and the utilization rate of replacement gilts is increased.

The invention discloses a method for inducing estrus and ovulation of a female cat, and belongs to the technical field of animal artificial propagation. The invention provides the method for inducingestrus synchronization and superovulation of the female cat in order to solve the problem that estrus and ovulation are difficult to synchronize in artificial propagation of the female cat. The methodspecifically comprises the following steps: continuously feeding adult healthy female cats with altrenogest with a dosage of 0.01-20mg / cat / day for 10-20 days, stopping feeding the altrenogest for 2 to 3 days, injecting 10-1000IU of pregnant mare serum hormone into the muscle of the female cat, and intramuscularly injecting 10-1000 IU of human chorionic gonadotropin after 72-96 hours, so that thefemale cat can perform estrus synchronization and superovulation within a preset time. The key technical difficulty of artificial breeding of the domestic cat or the feline is solved.

The invention provides a method for controlling estrus synchronization of maria by adopting traditional Chinese medicines. A medicine composition comprises the following raw medicines: Chinese angelica, motherwort herb, prepared rhizome of rehmannia, dried tangerine peel, white peony root, Chinese yam and dwarf lilyturf tuber. The medicine composition has the beneficial effects that the medicine composition takes warming the spleen and stomach for dispelling cold and benefiting qi and raising Yang as the treatment principles, is reasonable in composition, has simple preparation processes and has a wide range of application; the medicine composition is prepared from pure Chinese herbal medicines, has a wide source, is low in cost, has a high cure rate and does not have residues or toxic or side effect; in particular, plant cell walls are furthest destroyed after superfine grinding, thus overcoming the defects that traditional medicine powder has high content of crude fibers and active ingredients are hard to dissolve out and being easier to absorb by horses; the medicine composition has obvious functions of activating blood and adjusting body functions, effectively controls estrus synchronization of maria, has smaller toxic and side effects than western medicines, and does not have medicine residues.

The invention provides a recombinant equine chorionic gonadotropin fusion protein as well as a preparation method and application thereof. The recombinant equine chorionic gonadotropin fusion proteinspecifically comprises a reCG-Fc fusion protein, the reCG-Fc fusion protein contains an alpha subunit and a beta-FC subunit, the beta-FC subunit is formed through fusion of an eCG beta subunit and animmune globulin Fc segment, and the alpha subunit is connected with the beta-FC subunit through Van der Waals' force. The reCG-Fc fusion protein is expressed by using a mammal eukaryotic expression system. The reCG-Fc fusion protein prepared in the invention is used in the field of animal breeding, which comprises estrus synchronization and superovulation of dams; and compared with Pregnant Mare Serum Gonadotropin (PMSG) extracted from serum of pregnant mare at present, the recombinant equine chorionic gonadotropin fusion protein is more stable in quality, higher in purity, less in impuritiesand better in pesticide effect, and the fusion protein can be applied in animal breeding instead of PMSG.

The present invention discloses a feed additive for improving pregnancy rate and a preparation method thereof. The feed additive is composed of the following raw materials in parts by weight: 25-30 parts of corn, 10-20 parts of soybean meal, 5-10 parts of wheat bran, 15-20 parts of fish meal, 20-25 parts of chicken manure, 4-6 parts of a compound feed, 1.5-2.5 parts of actinolite powder, 2-7 partsof processed yeasts, 5-15 parts of plant alkaloids, 0.5-1 part of an attractant, 0.5-1 part of edible salt, 0.5-1 part of amino acid and 1-1.5 parts of calcium dihydrogen phosphate. The feed additiveeffectively and healthily reduces a phenomenon that the estrus synchronization rate of livestock is not high, has a positive effect on the pregnancy of the female livestock, and increases the numberof breeding.

The invention discloses a method for enhancing estrus synchronization effects of cattle embryo transfer and used drugs, and specifically provides applications of luteinizing hormone-releasing hormoneA3 to preparation of drug compositions for enhancing the estrus synchronization effects of cattle embryo transfer. The method includes the following steps: providing donor cattle; injecting the drugsto the muscle of the donor cattle; continuously observing the estrus and ovulation conditions of cows in 3 days from the day that the drugs are injected, recording the beginning and ending time of estrus of the cows, performing observing in every morning and evening, determining estrus time, estrus procedure and ovulation status by combining the appearance estrus symptoms of the cows with rectal examination, determining estrus synchronization if the cows show estrus in 3 days after the drugs are injected, and timely performing artificial insemination on the cows ovulating in heat according totechnical operating instructions of artificial insemination of frozen semen, wherein semen is frozen semen from the same donor cattle; and after 90 d of mating, determining whether the cows are pregnant by taking rectal examination results as bases, and performing feeding during pregnancy according to conventional methods.

The invention discloses a method for in-vitro cloned embryo transplantation of a pregnant sow. The method comprises the following steps: feeding an estrus synchronization agent to each sow at a fixedpoint every day, after continuously feeding each sow with the estrus synchronization agent for 18 days, performing intramuscular injection of PG600 5 days later, and selecting an individual with normal estrus for in-vitro cloned embryo transplantation 1 day later. According to the method, a proper microenvironment is provided for the pregnant sow after the cloned embryo is transplanted by virtue of feeding or injection of the estrus synchronization agent and the PG600, synchronism of the estrus, ovulation and embryo transplantation time of the pregnant sow is controlled, and the success rate of cloned embryo implantation and the pregnancy rate of the pregnant sow are greatly increased, so that batch production of cloned pigs is realized, and a foundation is laid for large-scale productionof the cloned pigs.

The invention relates to the technical field of cloned animals, in particular to a method for realizing batch production of cloned pigs by estrus synchronization with drugs. The method comprises the following steps: S1, preparing an altrenogest solution; S2, taking a small amount of pellet feed, sucking a proper amount of the altrenogest solution by using a 10 ml injector with a needle head, uniformly spraying the altrenogest solution on the feed, meanwhile shaking while spraying to enable the feed to fully absorb the solution, and feeding the feed into a trough of surrogate sows; S3, after waiting for a few minutes, checking the trough to ensure that all the feed containing allylpregnane is eaten by each medicated surrogate sow; S4, feeding the sows with a feed containing the allylpregnane for 14 days, then stopping feeding for 7 days, and injecting the sows with 2 ml of human chorionic gonadotrophin 1000 IU on the 18th day; and S5, observing oestrus of the surrogate sows on 20th dayto 21th day. The bottleneck of estrus asynchronization and ovulation synchronization of replacement sows is solved, and a foundation is laid for large-scale production of the cloned pigs.

The invention provides a method for performing synchronous estrus promotion on Yunba cattle with a Chinese herbal medicine preparation. The Chinese herbal medicine preparation is a Chinese herbal medicine decoction filtrate prepared from herba leonuri, pumpkin leaves and flos carthami in a reasonable proportion; the method for performing synchronous estrus promotion comprises the steps that the dosage for each head of cattle is calculated on principle that 1 parts of 250-260 ml of Chinese herbal medicine preparation is used per 100 kg of weight, and according to the calculated dosage, a cluster of female cattle to mate takes the Chinese herbal medicine preparation in such a way that feeding is carried out twice daily in the morning and evening for 3-4 days, and thus, the female cattle canenter the estrus, and then, mate and get pregnant. By employing the method, follicular phases of a cluster of female cattle come over the same period, and then, the female cattle enter synchronous estrus and ovulate, and thereby, the estrus cycles of a cluster of female cattle are artificially controlled and adjusted, and the female cattle enter estrus intensively within predetermined time so as to facilitate reasonable organization of mating of the cattle in a planned manner.

The invention relates to a method for cultivating a new meat goat strain / species of Guizhou black goat. The method comprises the following steps: 1) breeding Guizhou black goats, Nubian goats and Karahao goats; 2) obtaining a Nubian-black hybrid F1 generation female parent and a Karahao-Nubian hybrid F1 generation male parent; 3) obtaining a Karahao-Nubian-black F2 generation female parent; and 4)obtaining a Karahao-Nubian-black F3 generation male parent and a Karahao-Nubian-black F3 generation female parent, and fixing the excellent Karahao-Nubian-black F3 generation by crossing. According to the method, the BLUP method technology is combined with the conventional breeding method, and the new meat strain (species) with the blood relationship ratios of the Karahao goats, the Nubian goatsand the Guizhou black goats being 50%, 37.5% and 12.5% respectively is finally cultivated by virtue of the technical means of synchronous estrus, artificial insemination, superovulation, embryo transplantation and the like. The strain (species) can fully combine the heterosis of high meat yield of the Karahao goats, moderate meat yield and strong lactation capability of the Nubian goats, and retain the excellent characteristics of good meat quality, crude feed resistance and strong stress resistance of the Guizhou black goats.

The invention relates to the technical field of livestock raising and discloses a batch balanced production mode for piglets. The production mode comprises the following steps: acquiring quantities ofdelivery rooms and multiparous sows; according to the quantities of the delivery rooms and the multiparous sows, acquiring mating quantity of each batch, quantity of delivery clutches, quantity of weaned piglets and market selling quantity of batch balanced production of four weeks; performing estrus synchronization, all-in-all-out and hormone control according to the mating quantity of each batch, the quantity of the delivery clutches, the quantity of the weaned piglets and the market selling quantity. By combining precise pig breeding technology, hybridized combination determination and batch production technology, weaning period, shed vacancy period and delivery waiting period of breeding pigs are shortened, so that breeding pig production time is shortened effectively, equipment use ratio is increased, production cost is reduced, and market competitiveness of products is improved.

The invention provides a product for promoting oestrus of female mammals, the product comprises kiss-actuator (Kp) and gonadotropin releasing hormone (GnRH), and oestrus and conception of female animals can be remarkably promoted through the synergistic effect of the kiss-actuator (Kp) and the GnRH. The invention further provides a method for promoting oestrus of the female mammals, an effective dose of Kp is applied in the early oestrus period of the female mammals, then an effective dose of GnRH is applied, follicle development of the female mammals can be effectively promoted, the oestrus rate and conception rate of the female mammals are increased, the missed mating rate of the female mammals with recessive oestrus is reduced, and the oestrus of the female mammals is promoted. The device is suitable for being applied to the synchronous oestrus and timed insemination procedures of the female livestock.

The invention discloses an altrenogest injection and a preparation method and application thereof. The altrenogest injection is obtained by mixing altrenogest and a solvent, and the solvent is obtained by mixing dimethyl sulfoxide and sucrose acetate isobutyrate according to the mass ratio of 4 to 1. The prepared altrenogest sustained-release injection can realize in-vitro slow release, is good in stability, can reduce the administration frequency of pigs, reduces the labor intensity of administration work, and can effectively popularize a sow estrus synchronization technology.

The invention provides a method for improving reproductive performance of black goats. The effect of single-birth high yield of the goats is achieved through the combination of estrus synchronization and superovulation. The invention mainly relates to a breeding method for increasing the number of single-time lambing of goats, and aims to provide a breeding method for improving the efficient reproductive performance of black goats so as to achieve the purposes that the black goats can produce more birth in one birth, the number of lambing is increased, the breeding rate of the goats is increased, and the economic benefits of farmers are increased.

The invention discloses an ultrashort-period synchronous estrus method for sheep. The method comprises the following steps: selecting ewes capable of being bred; embedding a progesterone suppository in vaginas of the ewes capable of breeding, and recording the embedding day as the 0th day; carrying out intramuscular injection of cloprostenol sodium on the fourth day; withdrawing the progesterone suppository on the fifth day, and injecting PMSG intramuscularly at the same time; and 12-72 hours after the progesterone suppository is withdrawn, performing estrus test by using rams, and selecting estrus ewes. According to the method, ewes can be bred to have an estrus within 5 days, the embedding time is greatly shortened compared with the previous method that the progesterone suppository needs to be embedded for more than 10 days, the estrus rate is increased, and meanwhile, the suppository falling rate and the vaginitis of the ewes are effectively reduced.

The invention relates to the field of embryo breeding of live pigs, and particularly discloses an embryo breeding method and device based on live pigs. According to a technical scheme in the invention, the invention comprise the following steps: step 1, selecting a donor and a receptor, wherein a sow should be 0.8-6 years old and has a body weight of greater than 50 kg; and step 2, carrying out comprehensive physical examination, estrus rule recording and estrus synchronization treatment on the donor and the receptor. According to the invention, artificial intervention can be carried out on mating of boars and sows and screening and breeding of embryos, and high-quality embryos are selected for culture, so the reproductive survival rate of live pigs and the quality of piglets are improved, breeding quality is ensured while the breeding efficiency of the live pigs is improved, genes of the live pigs are selectively optimized, breeding capability and demands are enhanced, the success rate of in-vitro embryo culture and preservation is increased, and a certain protection effect is provided for in-vitro embryo culture and preservation.

The invention relates to the technical field of livestock breeding and in particular relates to a cattle estrus synchronization method. According to the method, progesterone pessaries, PGF2alpha analogue and a cattle estrus synchronization traditional Chinese medicine composition consisting of Angelica sinensis, motherwort, processed rehmannia roots, Chinese yams, dates, glossy privet fruits, dutchmanspipe roots and radix ophiopogonis through reasonable matching are used together, times and conditions of different steps in a cattle estrus synchronization period are controlled, in addition, variation of hormone levels in cow bodies is sufficiently controlled, development and ovulation of ovarian follicle are promoted, and then an effect of estrus synchronization can be achieved; in addition, by adopting the method, a small amount of medicines are used before and in ovulation, so that a small amount of medicines are left in cow bodies, later embryonic development is slightly affected, and the method is a cattle estrus synchronization method which is based on both traditional Chinese methods and western methods and has a small amount of traditional Chinese medicine residues in cow bodies and embryos.

The invention provides a method for increasing the parturition rate of mustela pulourius furo. The method for increasing the parturition rate of the mustela pulourius furo comprises the following steps: performing superovulation treatment on female mustela pulourius furo; carrying out illumination control treatment on male mustela pulourius furo; and mating the female mustela pulourius furo with the male mustela pulourius furo to obtain offsprings. According to the method, reproductive hormone is injected into the body of the female mustela pulourius furo, superovulation of the female mustela pulourius furo is promoted, the breeding period is adjusted, meanwhile, lamplight is used for simulating changes of the natural illumination period, the male mustela pulourius furo is induced to estrus at the same time, and therefore the effect of increasing the litter size is achieved, and conditions are created for multiple times of breeding of the mustela pulourius furo within one year. According to the method for increasing the parturition rate of the mustela pulourius furo, the obstacle that the mustela pulourius furo can only breed once in one year in a natural state is overcome, the parturition quantity of each fetus is increased, more convenient conditions are provided for related research, and the application range is very wide.