Method for inhibiting proliferation and myogenic differentiation of bovine skeletal muscle satellite cells by interfering UBA2 expression

A technology of satellite cell and myoblast differentiation, applied in the fields of biology, cell and tissue engineering, can solve the problems of undiscovered patent publications and other issues

Active Publication Date: 2021-10-15
TIANJIN AGRICULTURE COLLEGE
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  • Abstract
  • Description
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[0004] Through the search, no patent publications related to

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  • Method for inhibiting proliferation and myogenic differentiation of bovine skeletal muscle satellite cells by interfering UBA2 expression
  • Method for inhibiting proliferation and myogenic differentiation of bovine skeletal muscle satellite cells by interfering UBA2 expression
  • Method for inhibiting proliferation and myogenic differentiation of bovine skeletal muscle satellite cells by interfering UBA2 expression

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Embodiment Construction

[0020] The embodiments of the present invention will be described in detail below. It should be noted that the embodiments are illustrative, not restrictive, and cannot limit the protection scope of the present invention.

[0021] The raw materials used in the present invention, unless otherwise specified, are conventional commercially available products; the methods used in the present invention, unless otherwise specified, are conventional methods in the art.

[0022] A method for suppressing the proliferation and myogenic differentiation of bovine skeletal muscle satellite cells by interfering with UBA2 expression, the method is to suppress the proliferation and myogenic differentiation of bovine skeletal muscle satellite cells by interfering with UBA2 expression, wherein the UBA2 genome The base sequence is: SEQ NO.1.

[0023] Preferably, the specific steps are as follows:

[0024] According to the base sequence of UBA2, si-RNA of UBA2 was designed and synthesized, and bo...

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Abstract

The invention discloses a method for inhibiting proliferation and myogenic differentiation of bovine skeletal muscle satellite cells by interfering UBA2 expression, the method realizes inhibition of proliferation and myogenic differentiation of bovine skeletal muscle satellite cells by interfering UBA2 expression, and the genome base sequence of UBA2 is SEQ NO.1. According to the method, the proliferation and myogenic differentiation process of the bovine muscle satellite cells is regulated and controlled by changing UBA2 expression, enlightenment can be provided for research of ubiquitin-like modifier activating enzyme for muscle development and differentiation, and a new thought and reference are provided for clinical research and diagnosis and treatment of muscle development and differentiation and damage repair.

Description

technical field [0001] The invention belongs to the technical field of biology, cell and tissue engineering, in particular to a method for inhibiting bovine skeletal muscle satellite cell proliferation and myogenic differentiation by interfering with UBA2 expression. Background technique [0002] Skeletal muscle satellite cells (skeletal muscle satellite cells) are flat, protruding cells attached to the surface of muscle fibers in skeletal muscle. They usually exist between the sarcolemma and basement membrane in a resting state. Under certain conditions, they can When activated, muscle satellite cells can proliferate, differentiate, and fuse into mature myotubes or muscle fibers. Muscle satellite cells play a very important role in the growth, development and regeneration of muscles in animals after birth. The in vitro proliferation and myogenic differentiation process of muscle satellite cells can well simulate the muscle development process in vivo, and it is a good cell ...

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Application Information

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IPC IPC(8): C12N5/10C12N5/077C12N15/113
CPCC12N5/0659C12N15/113C12N9/00C12N2310/141
Inventor 丁向彬张钰婷于州王轶敏张林林李新郭益文郭宏
Owner TIANJIN AGRICULTURE COLLEGE
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