Construction method of oxidative damage model for muscle cell of beef cattle and application of oxidative damage model

Abstract

The invention provides a construction method of an oxidative damage model for a muscle cell of a beef cattle and the application of the oxidative damage model, and belongs to the technical field of cytobiology. The invention adopts the technical scheme that the construction method of the oxidative damage model for the muscle cell of the beef cattle comprises the following steps: preparing a cell culture fluid, separating a beef cattle muscle tissue, culturing the muscle cell of the beef cattle, and acquiring the construction parameter of the oxidative damage model. The construction method hasthe beneficial effects that the separated muscle cell of the beef cattle is taken as the object of study, the oxidative damage model for the muscle cell of the beef cattle is established through hydrogen peroxide, the materials are provided for studying the reduction of the beef quality and studying the nutrition control mechanism, the research and development costs for the subsequent tests are reduced; the established oxidative damage model for the muscle cell of the beef cattle provides the mechanism studying materials for studying the control mechanism of the beef quality and developing anantioxidant active material, and the time and the cost for researches and development are greatly shortened and reduced by directly applying the model.

Description

technical field [0001] The invention relates to the field of cell biology and technology, in particular to a method for constructing a beef cattle muscle cell oxidative damage model and its application. Background technique [0002] With the improvement of people's living standards, beef has entered thousands of households, but the quality varies widely. It is attributed to the reduction of beef quality caused by the stress caused by improper management and transportation of beef cattle during the process of feeding and slaughtering. At present, this problem has attracted widespread attention, but most researches are still only at the production level. In addition, many scholars and experts plan to add antioxidant active substances to beef cattle feed to improve beef quality, but due to the lack of molecular and cellular mechanism research, the dosage of additives is blind, and the negative effects of feeding are difficult to predict. At present, the oxidative damage model...

AI Technical Summary

Problems solved by technology

It is attributed to the reduction of beef quality caused by the stress caused by improper management and transportation of beef cattle during the process of feeding and slaughtering

At present, this problem has attracted widespread attention, but most studies are still only at the production level

In addition, many scholars and experts plan to add antioxidant active substances to beef cattle feed to improve beef quality, but due to the lack of molecular and cellular mechanism research, the dosage is blind, and the negative effects of feeding are difficult to predict

At present, the oxidative damage model of beef cattle muscle cells has not been established in China

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