59results about How to "Maintain dryness" patented technology

The invention provides water-based benzoic acid modified alkyd resin for an air-dry paint and a preparation method of the water-based benzoic acid modified alkyd resin and belongs to the technical field of paints. The water-based benzoic acid modified alkyd resin is prepared from the following raw materials of oleic acid, benzoic acid, polyhydric alcohol, polybasic acid, a catalyst, trimellitic anhydride, ethylene glycol butyl ether and sec-butyl alcohol at the mass ratio of (20-35):(5-20):(20-30):(15-30):(0.01-0.05):(4-10):(8-12):(6-8), wherein the oleic acid is any one or more of soya oil acid, tall oil acid, linoleic acid, linseed oil acid, calophyllum inophyllum oleic acid and dehydrated ricinolic acid. According to the water-based benzoic acid modified alkyd resin for the air-dry paint and the preparation method of the water-based benzoic acid modified alkyd resin, the prepared water-based benzoic acid modified alkyd resin has the beneficial effects of short surface drying time, high hardness and excellent water resistance and yellowing resistance.

The invention belongs to the technical field of stem cells, and relates to an ex-vivo expansion culture medium of umbilical cord blood hematopoietic stem cells and an application thereof. The ex-vivo expansion culture medium comprises an IMEM basal culture medium, serum, thrombopoietin, stem cell factors, FMS-liketyrosinc kinase 3 ligand, interleukin-1, interleukin-6, stem cell growth factors and berberine. The ex-vivo expansion culture medium of the umbilical cord blood hematopoietic stem cells has the advantages of being high in hematopoietic stem cell proliferation rate, capable of significantly improving the implantation capacity of the hematopoietic stem cells transplanted into a receptor and the reconstruction capacity of a hematopoietic system and capable of well maintaining the properties of the hematopoietic stem cells and is an ideal ex-vivo expansion culture medium of the umbilical cord blood hematopoietic stem cells.

The invention provides an organoid culture medium material and a preparation method and application thereof. The preparation method of the organoid culture medium material comprises the following steps: dissolving type I collagen, type IV collagen, laminin, hyaluronic acid and heparin with an acetic acid solution to obtain a collagen solution; and adding sodium dihydrogen phosphate, growth factors, A83-01 and Y27632 into a high-glucose DMEM / serum-reduced DMEM mixed nutrient solution, uniformly mixing the substances, uniformly mixing the mixture with the collagen solution, and regulating the pHvalue to 7.2-8.3 by using NaOH, thereby obtaining the product. When the organoid culture medium material is used for culturing partial types of organoids, the cell proliferation rate is higher than the cell proliferation rate existing when Matrigel is used as the medium material for culturing, the dryness of the organoids can be better maintained in the organoid culture and passage process, and the price is far lower than that of a general organoid culture material Matrigel.

Provided is a muscle stem cell in vitro culture method. A muscle stem cell is cultivated in vitro by using a cell culture medium of a cell factor added with a blood cell or a conditioned medium of the blood cell. Also provided are a culture medium used in the muscle stem cell in vitro culture method and an application thereof.

The invention provides a human amnion membrane epithelial cell culture medium and application. The culture medium is prepared from (1) an improved MCDB153 basic culture medium, (2) a serum substitution composition, (3) a cell growth factor composition and (4) a micromolecular substance. The culture medium is free of serum or plasma (including animal or human serum or plasma), so that the risk of zoonosis caused by animal serum or plasma in a cell therapy process can be avoided by adoption of the system for human epithelial cell culture. In addition, the culture medium is clear in component, and batch differences caused by use of blood products such as serum, plasma, blood platelets or the like are avoided. The culture medium is suitable for culture of human epithelial cells including amnion membrane epithelial cells and has a promising application prospect in researches of tissue engineering and the like.

The invention provides an amphiphilic polymer for promoting stem cell interface adhesion growth and a preparation method and use thereof. A hydrophilic component and a hydrophobic component are introduced into the amphiphilic polymer, wherein the hydrophobic component is used for forming a stable surface cover with a hydrophobic surface of a material through a hydrophobic effect, and the hydrophilic component provides biocompatibility and carries a polypeptide that promotes stem cell adhesion growth. The amphiphilic polymer is used for carrying out surface modification treatment on a hydrophobic base material, so that the hydrophobic base material has good biocompatibility and can promote long-time attachment growth of stem cells and maintain the dryness of the stem cells.

The invention discloses a thyroid organoid culture medium and a thyroid organoid culture and passage method. The thyroid organ culture medium comprises an Advanced DMEM (Dulbecco's Modified Eagle Medium) / F12 culture medium and the following components: B-27, N-acetylcysteine, nicotinamide, R-spondin1 recombinant protein, head protein, Wnt-3a, an epidermal growth factor, A83-01, a thyroid stimulating hormone, hydrocortisone, insulin, a fibroblast growth factor-10 and Y27632. The thyroid stimulating hormone, the hydrocortisone and the insulin are innovatively added into the thyroid organ culture medium, the thyroid organ formation can be remarkably promoted by adding the thyroid stimulating hormone, the cell proliferation and growth effect can be effectively promoted by adding the hydrocortisone, the clone formation rate can be increased, and the culture medium has the advantages that the cost is reduced; by adding insulin, the dryness of cells can be maintained, the passage efficiency is improved, and the thyroid organ culture medium can be used for culturing thyroid organs very well.

The invention belongs to the technical field of biology and discloses a serum-free culture medium for neural stem cells. The serum-free culture medium contains a basic culture medium, a nutritional additive, a growth factor, cytokines and an antioxidant. The cytokines are at least one of LIF and IL-6. The antioxidant is ascorbic acid. The culture medium is simple in formula and low in cost, can beused for in-vitro culture of neural stem cells, remarkably reduces the apoptosis rate of the neural stem cells in in-vitro culture, improves the proliferation rate of the neural stem cells, effectively reduces the apoptosis rate of the neural stem cells in in-vitro culture, can be used for maintaining the long-time in-vitro culture of the neural stem cells, and maintains the dryness of the neuralstem cells and the self-renewal capacity after long-time in-vitro culture.

The invention discloses a polyethylene-titanium oxide micro-nano multi-level structure composite microsphere material and its application. The polyethylene-titanium oxide micro-nano multi-level structure composite microsphere material of the present invention is made by immersing polyethylene microspheres in tetrachloride In the titanium-hydrochloric acid mixture, stir and react in a constant temperature water bath at 60-100°C for 4-24h, wash, and dry to obtain the product. The microsphere has a stable structure and uniform size of 100-500 μm, and the titanium oxide nanorod has a length of 10-50 nm. Seeding human adipose-derived mesenchymal stem cells on the microspheres can promote the proliferation of stem cells and maintain the stemness of stem cells.

The invention discloses a preparation method of mesenchymal stem cells applied to menopausal symptoms. The preparation method comprises the following steps: S1, separating; S2, initial treatment; S3,filtering; S4, inoculating and culture; S5, passage; S6, cell cryopreservation; and S7, cell thawing. The invention aims at carrying out uniform and systematic monitoring on the whole process from rawmaterial collection to completion of cell preparation, and the quality from screening before collection of the placenta tissue to preparation of cells into intermediate goods and final products of finished products is completely controlled.

The invention discloses a three-dimensional culture method of tumor stem cells. A honeycomb-shaped three-dimensional culture scaffold is 3D printed by using a photosensitive resin as a raw material. 2 The polycaprolactone of the S sustained-release donor JK1 was electrospun at the bottom of the culture scaffold, and the surface of the electrospun membrane was modified with growth factors to prepare a three-dimensional culture scaffold, which was then used to culture tumor stem cells. In the present invention, photosensitive resin is selected as the material of the 3D printing bracket, which has good biocompatibility and high mechanical strength; 2 The S donor JK1 is incorporated into electrospinning, and the pH of the medium can be changed by changing the pH of the medium. 2 The release of S is regulated to regulate the growth rate of cells; the growth factor is modified on the electrospinning surface to promote the proliferation of cancer stem cells. The invention utilizes the membrane-covered honeycomb culture scaffold to culture tumor stem cells with high quality and high efficiency, effectively promotes the proliferation of tumor stem cells, maintains their stemness and avoids differentiation, and lays a foundation for the characteristic research of tumor stem cells and the development of targeted drugs.

The invention relates to a hepatic stem cell injection and a preparation method thereof. The injection consists of liver stem cells, umbilical cord mesenchymal stem cells, polyethylene glycol, arginine, lysine, methionine, valine, glacial acetic acid, collagen, glycosaminoglycans, retinoic acid and Ethanolamine is composed according to a certain weight ratio. In the liver stem cell injection of the present invention, liver stem cells and umbilical cord mesenchymal stem cells are combined to have a better therapeutic effect on diabetes; the injection formula of the present invention can maintain the activity of liver stem cells for a long time without any side effects The composition is safe and effective, and the cost is low, and it can be directly applied to clinical injection of diabetes. Using the method for culturing hepatic stem cells and its special medium of the present invention can effectively maintain the optimal state of hepatic stem cells, guide cell proliferation, maintain the stemness of hepatic stem cells, and reduce the damage rate of hepatic stem cells in the process of culturing and harvesting and mortality, shortening the growth cycle.