59results about How to "Maintain dryness" patented technology

The invention relates to a gel preparation for maintaining the activity of cryopreserved cells and a stem cell gel preparation containing stem cells. The gel preparation comprises the following components by the mass percentage: 1-3% of sodium alginate, 1-5% of dimethyl sulfoxide, 1-5% of propylene glycol, 1-5% of epigallocatechin gallate, 2-10% of dextran, 1-5% of human blood albumin, and water or a phosphate solution added to make the total amount be 100%. The gel preparation has the advantages of good biocompatibility and simple operation, can maintain high activity of cells, can be directly used for low temperature preservation, and has no need of traditional liquid nitrogen cryopreservation; the cell gel preparation after resuscitation can still maintain high activity of the cells and dryness of the cells, and can be applied for treatment of skin injury or mucosal injury.

The invention provides an umbilical cord mesenchymal stem cell injection with an anti-aging function and a preparation method thereof. The injection comprises the following components: umbilical cord mesenchymal stem cell (5*10<5> to 10*10<5> / mL), 2 wt% of human albumin, and 98 wt% of compound electrolyte solution. The produced umbilical cord mesenchymal stem cells can rapidly repair and replace damaged cells and tissues, and have the characteristics of amplification and directional cell differentiation; and after the stem cells receive the signals from damaged parts, the stem cells will move to the damage parts, amplify in the damage parts, carry out induced differentiation, promote the self recovery function of stem cells, rapidly repair and replace damaged cells and tissues, rapidly secrete cell factors, growth factors, and tissue related factors in time, thus improve the blood circulation, enhance the metabolism functions, improve the tissue repairing function and immunity, and finally achieve the anti-aging goal.

The invention provides a preparation method of clinical mesenchymal stem cells. The method comprises screening puerperas before umbilical cord collection; taking healthy and mature volunteer puerperas as collecting targets; then performing separation, amplification and large-scale culture till the third generation, performing cryopreservation, and performing a series of quality detection on intermediate products generated during the separating process and the freezing process. When the frozen cells revive and are washed for standby, no obvious decrease of the viability occurs. The preparation method of the clinical mesenchymal stem cells aims at clinical, unified and systematic monitoring on the entire process from raw material collection to cell preparation accomplishment to achieve clinical standard product of the mesenchymal stem cells.

The invention relates to an efficient amplification culture system for human vascular endothelial progenitor cells and provides an efficient amplification culture method for endothelial progenitor cells, giving effective and mass amplification to CD34+ mononuclear cells at the premise of keeping their original dryness. The inventor also optimizes an endothelial growth factor combination attachment culture technique to amplify and differentiate endothelial progenitor cells. The technical scheme of the invention may provide mass endothelial progenitor cells and solve the source and quantity problems of endothelial progenitor cells or endothelial cells of a patient with ischemic disease and hemophilia A during transplantation therapy.

The invention provides water-based benzoic acid modified alkyd resin for an air-dry paint and a preparation method of the water-based benzoic acid modified alkyd resin and belongs to the technical field of paints. The water-based benzoic acid modified alkyd resin is prepared from the following raw materials of oleic acid, benzoic acid, polyhydric alcohol, polybasic acid, a catalyst, trimellitic anhydride, ethylene glycol butyl ether and sec-butyl alcohol at the mass ratio of (20-35):(5-20):(20-30):(15-30):(0.01-0.05):(4-10):(8-12):(6-8), wherein the oleic acid is any one or more of soya oil acid, tall oil acid, linoleic acid, linseed oil acid, calophyllum inophyllum oleic acid and dehydrated ricinolic acid. According to the water-based benzoic acid modified alkyd resin for the air-dry paint and the preparation method of the water-based benzoic acid modified alkyd resin, the prepared water-based benzoic acid modified alkyd resin has the beneficial effects of short surface drying time, high hardness and excellent water resistance and yellowing resistance.

The invention discloses a separation and cultivation method of human adipose stem cells. The method comprises the following steps: first, digesting a fat suction matter by using a Liberase digestive enzyme solution, neutralizing digestive enzyme after digestion is ended, centrifuging, filtering, and further removing parenchyma cells by using a lymphocyte separation solution so as to obtain the human adipose stem cells; second, cultivating the human adipose stem cells by using a serum-free medium, wherein following components are added in the serum-free medium: a recombinant human epidermal growth factor, a recombinant human basic fibroblast growth factor, a recombinant human transforming growth factor-beta, a recombinant human platelet-derived growth factor-BB, a recombinant human stem cell factor, reduced glutathione, coenzyme A, biotin, MEM vitamin solution, MEM amino acid solution, MEM non-essential amino acid solution, a GlutaMAX additive, insulin-transferrin-selenium solution andgentamicin. The method can remarkably increase the yield of adipose stem cells and improve the activity of the adipose stem cells, so as to obtain more adipose stem cells with proliferation ability.

The invention belongs to the technical field of stem cells, and relates to an ex-vivo expansion culture medium of umbilical cord blood hematopoietic stem cells and an application thereof. The ex-vivo expansion culture medium comprises an IMEM basal culture medium, serum, thrombopoietin, stem cell factors, FMS-liketyrosinc kinase 3 ligand, interleukin-1, interleukin-6, stem cell growth factors and berberine. The ex-vivo expansion culture medium of the umbilical cord blood hematopoietic stem cells has the advantages of being high in hematopoietic stem cell proliferation rate, capable of significantly improving the implantation capacity of the hematopoietic stem cells transplanted into a receptor and the reconstruction capacity of a hematopoietic system and capable of well maintaining the properties of the hematopoietic stem cells and is an ideal ex-vivo expansion culture medium of the umbilical cord blood hematopoietic stem cells.

The invention relates to a multifunctional bioreactor used for cell culture and cell sorting. The multifunctional bioreactor comprises a turnover device and a cell culture vessel, wherein the turnover device contains a turnover support which can operate around a space axis and a magnetic field generating device in physical connection with the turnover device, the cell culture vessel is detachably fixed on the turnover support, the cell culture vessel is located on the space axis or an extension line thereof and operates along with the turnover support according to certain rules and tracks, the end face of the cell culture vessel is close to the magnetic field generating device, and the magnetic field generating device can control change of a magnetic field surrounding the periphery and interior of the cell culture vessel. The multifunctional bioreactor provided by the invention has the advantages that co-culture on adherent cells and suspension cells is carried out without replacing the vessel, stirring inside a culture solution is realized by virtue of movement of carriers in the vessel in a magnetic field environment, cell contamination, target cell damage and death risk are reduced, and the vessel can be taken as a bearing material of the adherent cells, so that cell culture efficiency is greatly improved.

The invention belongs to the technical field of stem cells, in particular to a method for large-scale expansion of mesenchymal stem cells. The method provided by the invention adopts a biodegradable gelatin microcarrier and combines with an intermittent stirring method to carry out suspension culture and jointly promotes the culture of mesenchymal stem cells. At the same time, in the cell cultureprocess, the serum-free medium is used instead of animal serum medium as the cell culture medium, which will not bring any mycoplasma and virus, thus avoiding the risk of virus infection. The method of the invention is simple in operation and low in cost, and can not only promote the culture of mesenchymal stem cells, but also maintain cell dryness and differentiation potential.

The invention provides an application of an exosome derived from a carcass in a skin regulation product. Experiments prove that the exosome realizes an anti-aging function by promoting cell collagen expression, increasing cell myofibrillar protein synthesis, maintaining stem cell dryness and stimulating stem cell proliferation; the exosome realizes a pharmaceutical function of anti-acne by inhibiting products of gene expression of inflammatory mediators or matrix protease in keratinocytes and inhibiting the synthesis and release of VEGF in keratinocytes; the exosome realizes a dermatological application of enhancing hair follicles and resisting alopecia by promoting ATP synthesis in hair follicle papilla fibroblasts or products for enhancing mitochondrial metabolic activity, increasing cell viability of micro hair follicles or reducing the damage of micro hair follicle membranes; and the exosome provides a new approach for skin care such as anti-aging skin care, acne resistance, alopecia prevention, dust impact prevention and the like by removing and eliminating toxins, increasing the expression of skin antimicrobial peptide hBD3, inhibiting the production of active oxygen and preventing skin problems caused by Asian dust.

The invention discloses an umbilical cord blood stem cell injection solution with an anti-aging effect and a preparation method thereof. The umbilical cord blood stem cell injection solution contains the following components by volume or concentration: 5X10<5> to 10X10<5> umbilical cord blood stem cells per ml, 2wt% of human albumin, 10-20v% of umbilical cord blood autologous plasma and 78-88v% of a compound electrolyte solution. According to the umbilical cord blood stem cell injection solution, the used stem cells are blood related stem cells and have the characteristics of amplification and directional differentiation; the cells return to an injured part when receiving an injured part signal; the cells are subjected to amplification and induced differentiation at the injured part and the stem cells of the injured part are promoted to recover functions, thereby quickly repairing and replacing injured cells and tissues; and cell factors, growth factors and tissue related factors are secreted quickly in time, thereby improving blood circulation of a human body, metabolic function, tissue repair capability and immunity, and further achieving the purpose of aging resistance.

A dental pulp stem cell resuscitation fluid and a dental pulp stem cell resuscitation method are disclosed, the new stem cell resuscitation fluid is constructed by addition of a certain amount of basic fibroblast growth factors into a conventional stem cell culture medium, the new stem cell resuscitation fluid is used in resuscitation process of DPSCs (dental pulp stem cells) for rapid recovery of activity of the DPSCs in a short period of time, stemness and multiple differentiation potential of the original stem cells can be maintained, the resuscitation time of the stem cells can be shortened, the clinical use survival rate of the stem cells can be improved, the proliferation rate exceed that of a conventional medium group, meanwhile the cell stemness and multiple differentiation potential can be maintained, seed cells with better performance and status can be effectively provided for clinical stem cell therapy, and a lot of time, manpower and material cost can be saved.

The invention discloses a preparation method of deciduas mesenchymal stem cells. The preparation method comprises the following steps: step 1, separation; step 2, initial treatment; step 3, filtration; step 4, inoculation and culture; step 5, passage; step 6, cell cryopreservation; step 7, cell thawing. According to the preparation method, the entire process from the start of raw material collection to completion of cell preparation is concentratedly and systematically monitored, and the quality from the start of screening before placenta collection to cellpreparation of a finished intermediate product and a final product is all controlled.

The invention relates to the technical field of water-based paint, in particular to a high-gloss high-DOI waterborne polyurethane coating and a preparation method and application thereof. The coatingcomprises a component A and a component B, wherein the component A is prepared from the following raw materials in parts by weight: 40-50 parts of water-based hydroxyl acrylic acid dispersion, 15-25 parts of water-based polyester dispersion, 5-8 parts of cosolvent, 3.5-4.5 parts of auxiliaries, 4-25 parts of pigment and 6.5-19 parts of deionized water. The component B is prepared from the following raw materials in parts by weight: 17.5 to 20 parts of waterborne isocyanate and 5 to 7.5 parts of a PGDA solvent. The water-based hydroxyl acrylic acid dispersion is matched with the water-based polyester dispersion to serve as matrix resin, and the leveling property and DOI value of a paint film are improved on the premise that weather resistance, chemical resistance, dryness and the like are guaranteed.

Provided is a muscle stem cell in vitro culture method. A muscle stem cell is cultivated in vitro by using a cell culture medium of a cell factor added with a blood cell or a conditioned medium of the blood cell. Also provided are a culture medium used in the muscle stem cell in vitro culture method and an application thereof.

The invention discloses a preparation method of cord blood monocytes. The preparation method comprises the following steps: 1, conducting preliminary screening and preserving of umbilical cord blood;2, implementing cultivation; 3, implementing absorbing; 4, implementing collecting; 5, conducting cell culture; 6, freezing and detecting monocytes; and 7, using the monocytes. The preparation methodprovided by the invention tries to achieve unified and systematic monitoring over a whole process from raw material collection to completion of cell preparation, so that a standard product from separation and freezing of the cord blood monocytes is obtained.

The invention relates to the technical field of steam cell culture, in particular to a cell culture medium and application in umbilical cord mesenchymal stem cell culture. EGF, NEAA, glutamine and a conditioned culture medium are added into a basic culture medium, and all components together play a role in well promoting growth of dental pulp stem cells. Experiments show that by adopting the culture medium to culture umbilical cord mesenchymal stem cells, the cell cycle can be shortened, the cell proliferative capacity is improved, the purity and dryness of the stem cells are improved, the culture medium does not contain animal origin components such as fetal calf serums, and the stem cells obtained through the culture medium are suitable for clinical application.

The invention relates to a zinc oxide nanorod array cell culture substrate, and a preparation method and application thereof. An electric conduction substrate coated with an aluminum foil is put in anaqueous solution of zinc nitrate hexahydrate-hexamethylenetetramine to grow through a water bath method to obtain the zinc oxide nanorod array cell culture substrate. The preparation method is simple, and cost is low. A zinc oxide seed crystal layer does not need to be prepared on the substrate in advance, the diameter of a zinc oxide nanorod prepared on multiple substrates through the water bathmethod is 50-500nm, and the length of the zinc oxide nanorod is 0.5-5 [mu]m. The zinc oxide nanorod of a large size can not be easily taken and dissolved by cells, and can continuously take effects for a long time, and in addition, better cell adhesion is provided. The zinc oxide nanorod array cell culture substrate prepared with the preparation method disclosed by the invention has a good effecton maintaining the dry effect of dry / progenitor cells under a condition of long-term culture in vitro for 1-30 days or passage.

The invention provides a human amnion membrane epithelial cell culture medium and application. The culture medium is prepared from (1) an improved MCDB153 basic culture medium, (2) a serum substitution composition, (3) a cell growth factor composition and (4) a micromolecular substance. The culture medium is free of serum or plasma (including animal or human serum or plasma), so that the risk of zoonosis caused by animal serum or plasma in a cell therapy process can be avoided by adoption of the system for human epithelial cell culture. In addition, the culture medium is clear in component, and batch differences caused by use of blood products such as serum, plasma, blood platelets or the like are avoided. The culture medium is suitable for culture of human epithelial cells including amnion membrane epithelial cells and has a promising application prospect in researches of tissue engineering and the like.

The invention relates to a culture medium supplementing composition, a stem cell culture medium and a preparation method of the stem cell culture medium. In stem cell research, the inventor finds that reduced glutathione and vitamins, in particular vitamin E or / and vitamin E analogues, have the effect of maintaining stem cell proliferation ability and stemness, and when the culture medium supplementing composition containing reduced glutathione and vitamin E or / and vitamin E analogues is added to a basal culture medium, the stem cells actively cultured by the obtained culture medium can effectively maintain the proliferation ability and the undifferentiated state of the stem cells; meanwhile, the oxidative stress reaction of the stem cells is also reduced.

The invention discloses a thyroid organoid culture medium and a thyroid organoid culture and passage method. The thyroid organ culture medium comprises an Advanced DMEM (Dulbecco's Modified Eagle Medium) / F12 culture medium and the following components: B-27, N-acetylcysteine, nicotinamide, R-spondin1 recombinant protein, head protein, Wnt-3a, an epidermal growth factor, A83-01, a thyroid stimulating hormone, hydrocortisone, insulin, a fibroblast growth factor-10 and Y27632. The thyroid stimulating hormone, the hydrocortisone and the insulin are innovatively added into the thyroid organ culture medium, the thyroid organ formation can be remarkably promoted by adding the thyroid stimulating hormone, the cell proliferation and growth effect can be effectively promoted by adding the hydrocortisone, the clone formation rate can be increased, and the culture medium has the advantages that the cost is reduced; by adding insulin, the dryness of cells can be maintained, the passage efficiency is improved, and the thyroid organ culture medium can be used for culturing thyroid organs very well.

The invention belongs to the technical field of biology and discloses a serum-free culture medium for neural stem cells. The serum-free culture medium contains a basic culture medium, a nutritional additive, a growth factor, cytokines and an antioxidant. The cytokines are at least one of LIF and IL-6. The antioxidant is ascorbic acid. The culture medium is simple in formula and low in cost, can beused for in-vitro culture of neural stem cells, remarkably reduces the apoptosis rate of the neural stem cells in in-vitro culture, improves the proliferation rate of the neural stem cells, effectively reduces the apoptosis rate of the neural stem cells in in-vitro culture, can be used for maintaining the long-time in-vitro culture of the neural stem cells, and maintains the dryness of the neuralstem cells and the self-renewal capacity after long-time in-vitro culture.

The invention provides a culture method of CD34+ hematopoietic stem cells. The culture method of the CD34+ hematopoietic stem cells comprises the following steps: preparing a CD34+ hematopoietic stemcell complete medium, wherein the CD34+ hematopoietic stem cell complete medium comprises cytokines and chemical molecules, the cytokines comprise SCF, Flt-3L, TPO, IL-6 and LDL, and the chemical molecules comprise SR1 and UM171; providing umbilical cord blood, and separating PBMC from the umbilical cord blood; performing re-suspension on the PBMC by using the CD34+ hematopoietic stem cell complete medium, and carrying out shaking culture; and then, during culture period, daily adding fresh CD34+ hematopoietic stem cell complete medium, and carrying out culturing for 9-12 days.

The invention discloses a culture medium suitable for large-scale culture of clinical-grade neural stem cells. The culture medium is characterized by being prepared from the following components: 13 to 20g / L of a basic culture medium, 1 to 5ng / mL of an insulin growth factor-1, 1 to 3ng / mL of a transforming growth factor beta 1, 3 to 10ng / mL of an epidermal growth factor, 5 to 12ng / mL of fetal calf serum, 0.001 to 0.003mg / mL of sodium pyruvate, 0.01 to 0.03mM of glutamine, 120 to 220 microms of chloromethyl trimethyl germane modified adenosine, 5 to 50 microms of compound vitamin, 3 to 15 microms of hyperbranched polyamino acid, 1 to 7 microms of hyperbranched chitosan, 3 to 6 micrograms / mL of carbenicillin, 0.008 to 0.012mg / mL of sodium chloride and 0.008 to 0.012mg / mL of sodium bicarbonate. The invention further provides a preparation method of the culture medium suitable for the large-scale culture of the clinical-grade neural stem cells. The culture medium suitable for the large-scale culture of the clinical-grade neural stem cells, disclosed by the invention, has the advantages of high safety, good stem cell culture effect, good cell stability and stem property keeping effect and rapid amplification speed.

The invention discloses a polyethylene-titanium oxide micro-nano multi-level structure composite microsphere material and its application. The polyethylene-titanium oxide micro-nano multi-level structure composite microsphere material of the present invention is made by immersing polyethylene microspheres in tetrachloride In the titanium-hydrochloric acid mixture, stir and react in a constant temperature water bath at 60-100°C for 4-24h, wash, and dry to obtain the product. The microsphere has a stable structure and uniform size of 100-500 μm, and the titanium oxide nanorod has a length of 10-50 nm. Seeding human adipose-derived mesenchymal stem cells on the microspheres can promote the proliferation of stem cells and maintain the stemness of stem cells.

The invention discloses a preparation method of mesenchymal stem cells applied to menopausal symptoms. The preparation method comprises the following steps: S1, separating; S2, initial treatment; S3,filtering; S4, inoculating and culture; S5, passage; S6, cell cryopreservation; and S7, cell thawing. The invention aims at carrying out uniform and systematic monitoring on the whole process from rawmaterial collection to completion of cell preparation, and the quality from screening before collection of the placenta tissue to preparation of cells into intermediate goods and final products of finished products is completely controlled.

The invention discloses a three-dimensional culture method of tumor stem cells. A honeycomb-shaped three-dimensional culture scaffold is 3D printed by using a photosensitive resin as a raw material. 2 The polycaprolactone of the S sustained-release donor JK1 was electrospun at the bottom of the culture scaffold, and the surface of the electrospun membrane was modified with growth factors to prepare a three-dimensional culture scaffold, which was then used to culture tumor stem cells. In the present invention, photosensitive resin is selected as the material of the 3D printing bracket, which has good biocompatibility and high mechanical strength; 2 The S donor JK1 is incorporated into electrospinning, and the pH of the medium can be changed by changing the pH of the medium. 2 The release of S is regulated to regulate the growth rate of cells; the growth factor is modified on the electrospinning surface to promote the proliferation of cancer stem cells. The invention utilizes the membrane-covered honeycomb culture scaffold to culture tumor stem cells with high quality and high efficiency, effectively promotes the proliferation of tumor stem cells, maintains their stemness and avoids differentiation, and lays a foundation for the characteristic research of tumor stem cells and the development of targeted drugs.

The invention relates to a hepatic stem cell injection and a preparation method thereof. The injection consists of liver stem cells, umbilical cord mesenchymal stem cells, polyethylene glycol, arginine, lysine, methionine, valine, glacial acetic acid, collagen, glycosaminoglycans, retinoic acid and Ethanolamine is composed according to a certain weight ratio. In the liver stem cell injection of the present invention, liver stem cells and umbilical cord mesenchymal stem cells are combined to have a better therapeutic effect on diabetes; the injection formula of the present invention can maintain the activity of liver stem cells for a long time without any side effects The composition is safe and effective, and the cost is low, and it can be directly applied to clinical injection of diabetes. Using the method for culturing hepatic stem cells and its special medium of the present invention can effectively maintain the optimal state of hepatic stem cells, guide cell proliferation, maintain the stemness of hepatic stem cells, and reduce the damage rate of hepatic stem cells in the process of culturing and harvesting and mortality, shortening the growth cycle.