59results about How to "Maintain dryness" patented technology

The invention provides an organoid culture medium material and a preparation method and application thereof. The preparation method of the organoid culture medium material comprises the following steps: dissolving type I collagen, type IV collagen, laminin, hyaluronic acid and heparin with an acetic acid solution to obtain a collagen solution; and adding sodium dihydrogen phosphate, growth factors, A83-01 and Y27632 into a high-glucose DMEM / serum-reduced DMEM mixed nutrient solution, uniformly mixing the substances, uniformly mixing the mixture with the collagen solution, and regulating the pHvalue to 7.2-8.3 by using NaOH, thereby obtaining the product. When the organoid culture medium material is used for culturing partial types of organoids, the cell proliferation rate is higher than the cell proliferation rate existing when Matrigel is used as the medium material for culturing, the dryness of the organoids can be better maintained in the organoid culture and passage process, and the price is far lower than that of a general organoid culture material Matrigel.

Provided is a muscle stem cell in vitro culture method. A muscle stem cell is cultivated in vitro by using a cell culture medium of a cell factor added with a blood cell or a conditioned medium of the blood cell. Also provided are a culture medium used in the muscle stem cell in vitro culture method and an application thereof.

The invention provides an amphiphilic polymer for promoting stem cell interface adhesion growth and a preparation method and use thereof. A hydrophilic component and a hydrophobic component are introduced into the amphiphilic polymer, wherein the hydrophobic component is used for forming a stable surface cover with a hydrophobic surface of a material through a hydrophobic effect, and the hydrophilic component provides biocompatibility and carries a polypeptide that promotes stem cell adhesion growth. The amphiphilic polymer is used for carrying out surface modification treatment on a hydrophobic base material, so that the hydrophobic base material has good biocompatibility and can promote long-time attachment growth of stem cells and maintain the dryness of the stem cells.

The invention discloses a polyethylene-titanium oxide micro-nano multi-level structure composite microsphere material and its application. The polyethylene-titanium oxide micro-nano multi-level structure composite microsphere material of the present invention is made by immersing polyethylene microspheres in tetrachloride In the titanium-hydrochloric acid mixture, stir and react in a constant temperature water bath at 60-100°C for 4-24h, wash, and dry to obtain the product. The microsphere has a stable structure and uniform size of 100-500 μm, and the titanium oxide nanorod has a length of 10-50 nm. Seeding human adipose-derived mesenchymal stem cells on the microspheres can promote the proliferation of stem cells and maintain the stemness of stem cells.

The invention discloses a three-dimensional culture method of tumor stem cells. A honeycomb-shaped three-dimensional culture scaffold is 3D printed by using a photosensitive resin as a raw material. 2 The polycaprolactone of the S sustained-release donor JK1 was electrospun at the bottom of the culture scaffold, and the surface of the electrospun membrane was modified with growth factors to prepare a three-dimensional culture scaffold, which was then used to culture tumor stem cells. In the present invention, photosensitive resin is selected as the material of the 3D printing bracket, which has good biocompatibility and high mechanical strength; 2 The S donor JK1 is incorporated into electrospinning, and the pH of the medium can be changed by changing the pH of the medium. 2 The release of S is regulated to regulate the growth rate of cells; the growth factor is modified on the electrospinning surface to promote the proliferation of cancer stem cells. The invention utilizes the membrane-covered honeycomb culture scaffold to culture tumor stem cells with high quality and high efficiency, effectively promotes the proliferation of tumor stem cells, maintains their stemness and avoids differentiation, and lays a foundation for the characteristic research of tumor stem cells and the development of targeted drugs.