Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method for detecting the expression level of PPAR gamma gene in bovine muscle

A technology of γ gene and expression amount, applied in the field of genetic engineering, to achieve the effect of easy operation and simple method

Inactive Publication Date: 2019-04-19
INNER MONGOLIA AGRICULTURAL UNIVERSITY
View PDF5 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are few reports about this gene in bovine muscle

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for detecting the expression level of PPAR gamma gene in bovine muscle
  • Method for detecting the expression level of PPAR gamma gene in bovine muscle

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0020] In order to enable those skilled in the art to better understand the solution of the present invention, the technical solution of the present invention will be further described below in conjunction with specific examples.

[0021] A method for detecting the expression level of bovine muscle PPARγ gene, the specific steps are as follows:

[0022] 1) Design the PPARγ gene sequence specific primer pair P-F and P-R and the internal reference gene primer pair G-F and G-R:

[0023] Refer to the PPARγ gene sequence (Genbank Accession No.NM-181024) recorded in Genbank, and the internal reference gene GAPDH (Genbank Accession No.0NM-001034034), use the Primer Premier 3.0 software to design primers, and use the Blast function in NCBI to test the specificity of the primers .

[0024] P-F: 5'-GTGAAGCCCATTGAGGACAT-3', see SEQ NO.1 in the sequence listing.

[0025] P-R: 5'-AGCTGCACGTGTTCTGTCAC-3', see SEQ NO.2 in the sequence listing.

[0026] The sequence of the primer pair G-F ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a method for detecting the expression level of a PPAR gamma gene in bovine muscle. According to the method, the expression level of the PPAR gamma gene in the bovine muscle isaccurately and quickly analyzed through the design of a PPAR gamma gene-specific primer pair, the qPCR amplification of an internal reference primer GAPDH and relative quantitative analysis of a qPCRresult. The qPCR detection method for detecting the expression level of the PPAR gamma gene can be used for accurately detecting the expression level of the PPAR gamma genes in the muscle of cattle ofdifferent breeds. The method can be used for detecting the expression level of the PPAR gamma genes in the muscle of the cattle of different breeds, provides a reference for further research on the functions of the bovine PPAR gamma gene, and is simple and easy to operate.

Description

technical field [0001] The invention relates to the field of genetic engineering, in particular to a method for detecting the expression of bovine muscle PPARγ gene. Background technique [0002] Peroxisome proliferator-activated receptors (PPARs) are a class of ligand-activated nuclear transcription factors that belong to the transcription factor superfamily. PPARs have three subtypes of α, δ, and γ in mammals. As PPARγ, which is essential and plays a central role in the differentiation of adipocytes, it is mainly expressed in adipose tissue and the immune system, and has the ability to positively regulate adipocytes. The role of differentiation. Under the interaction of C / EBPs and other related adipokines, PPARγ participates in the proliferation and differentiation of adipocytes through multiple signaling pathways. Different species of animals have different mechanism of fat formation, therefore, the distribution of PPARγ gene in different species of animals is also diff...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12Q1/6851
CPCC12Q1/6851C12Q2563/107C12Q2545/101C12Q2531/113
Inventor 斯木吉德敖日格乐王纯洁
Owner INNER MONGOLIA AGRICULTURAL UNIVERSITY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com