Method for detecting the expression level of PPAR gamma gene in bovine muscle

A technology of γ gene and expression amount, applied in the field of genetic engineering, to achieve the effect of easy operation and simple method

Inactive Publication Date: 2019-04-19
INNER MONGOLIA AGRICULTURAL UNIVERSITY
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  • Description
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  • Method for detecting the expression level of PPAR gamma gene in bovine muscle
  • Method for detecting the expression level of PPAR gamma gene in bovine muscle

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Embodiment Construction

[0020] In order to enable those skilled in the art to better understand the solution of the present invention, the technical solution of the present invention will be further described below in conjunction with specific examples.

[0021] A method for detecting the expression level of bovine muscle PPARγ gene, the specific steps are as follows:

[0022] 1) Design the PPARγ gene sequence specific primer pair P-F and P-R and the internal reference gene primer pair G-F and G-R:

[0023] Refer to the PPARγ gene sequence (Genbank Accession No.NM-181024) recorded in Genbank, and the internal reference gene GAPDH (Genbank Accession No.0NM-001034034), use the Primer Premier 3.0 software to design primers, and use the Blast function in NCBI to test the specificity of the primers .

[0024] P-F: 5'-GTGAAGCCCATTGAGGACAT-3', see SEQ NO.1 in the sequence listing.

[0025] P-R: 5'-AGCTGCACGTGTTCTGTCAC-3', see SEQ NO.2 in the sequence listing.

[0026] The sequence of the primer pair G-F ...

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Abstract

The invention discloses a method for detecting the expression level of a PPAR gamma gene in bovine muscle. According to the method, the expression level of the PPAR gamma gene in the bovine muscle isaccurately and quickly analyzed through the design of a PPAR gamma gene-specific primer pair, the qPCR amplification of an internal reference primer GAPDH and relative quantitative analysis of a qPCRresult. The qPCR detection method for detecting the expression level of the PPAR gamma gene can be used for accurately detecting the expression level of the PPAR gamma genes in the muscle of cattle ofdifferent breeds. The method can be used for detecting the expression level of the PPAR gamma genes in the muscle of the cattle of different breeds, provides a reference for further research on the functions of the bovine PPAR gamma gene, and is simple and easy to operate.

Description

technical field [0001] The invention relates to the field of genetic engineering, in particular to a method for detecting the expression of bovine muscle PPARγ gene. Background technique [0002] Peroxisome proliferator-activated receptors (PPARs) are a class of ligand-activated nuclear transcription factors that belong to the transcription factor superfamily. PPARs have three subtypes of α, δ, and γ in mammals. As PPARγ, which is essential and plays a central role in the differentiation of adipocytes, it is mainly expressed in adipose tissue and the immune system, and has the ability to positively regulate adipocytes. The role of differentiation. Under the interaction of C / EBPs and other related adipokines, PPARγ participates in the proliferation and differentiation of adipocytes through multiple signaling pathways. Different species of animals have different mechanism of fat formation, therefore, the distribution of PPARγ gene in different species of animals is also diff...

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Application Information

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IPC IPC(8): C12Q1/6851
CPCC12Q1/6851C12Q2563/107C12Q2545/101C12Q2531/113
Inventor 斯木吉德敖日格乐王纯洁
Owner INNER MONGOLIA AGRICULTURAL UNIVERSITY
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