Method for detecting expression level of FASN gene in bovine muscle

A technology of gene expression and bovine muscle, applied in the field of genetic engineering, to achieve the effect of easy operation and simple method

Inactive Publication Date: 2019-04-19
INNER MONGOLIA AGRICULTURAL UNIVERSITY
View PDF5 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are few reports about this gene in bovine muscle

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for detecting expression level of FASN gene in bovine muscle
  • Method for detecting expression level of FASN gene in bovine muscle

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0021] In order to enable those skilled in the art to better understand the solution of the present invention, the technical solution of the present invention will be further described below in conjunction with specific examples.

[0022] A method for detecting bovine muscle FASN gene expression, the specific steps are as follows:

[0023] 1) Design the specific primer pair F-F and F-R of the FASN gene sequence and the primer pair G-F and G-R of the internal reference gene:

[0024] Referring to the FASN gene sequence (Genbank Accession No.NM-001012669) recorded in Genbank, and the internal reference gene GAPDH (Genbank Accession No.0NM-001034034), primers were designed using Primer Premier 3.0 software, and the specificity of the primers was tested through the Blast function in NCBI .

[0025] F-F: 5'-GTGAAGCCCATTGAGGACAT-3', see SEQ NO.1 in the sequence listing.

[0026] F-R: 5'-AGCTGCACGTGTTCTGTCAC-3', see SEQ NO.2 in the sequence listing.

[0027] The sequence of the pr...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a method for detecting the expression level of an FASN gene in bovine muscle. According to the method, the expression level of the FASN gene in the bovine muscle is accuratelyand quickly analyzed through the design of an FASN gene-specific primer pair, the qPCR amplification of an internal reference primer GAPDH and relative quantitative analysis of a qPCR result. The qPCRdetection method for detecting the expression level of the FASN gene can be used for accurately detecting the expression level of the FASN genes in the muscle of cattle of different breeds. The method can be used for detecting the expression level of the FASN genes in the muscle of the cattle of different breeds, provides a reference for further research on the functions of the bovine FASN gene,and is simple and easy to operate.

Description

technical field [0001] The invention relates to the field of genetic engineering, in particular to a method for detecting the expression of bovine muscle FASN gene. Background technique [0002] Fatty acid synthase (FASN) is a multi-enzyme complex system composed of 6 enzymes. FASN is the rate-limiting enzyme for fat synthesis, and its activity directly controls the synthesis of body fat. In mammals, only the FASN in dimer form has the activity of catalyzing fatty acid synthesis, and the monomer form of FASN does not have the activity of catalyzing fatty acid synthesis, but some functional domains of FASN still have catalytic activity in the monomer. The substrate is transferred to the acyl carrier protein (Acyl carrier protein, ACP) functional domain in advance and the activity of the functional domain interacting with ACP is reduced. [0003] Using the methods of in situ hybridization and somatic cell hybridization, the bovine FASN gene was located on chromosome 19. The f...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C12Q1/6888C12Q1/6851
CPCC12Q1/6851C12Q1/6888C12Q2600/158C12Q2531/113C12Q2545/101C12Q2563/107
Inventor 敖日格乐斯木吉德王纯洁
Owner INNER MONGOLIA AGRICULTURAL UNIVERSITY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap