322 results about "Cell method" patented technology

Method for informing a mobile terminal (MN), which is located in a first cell, on system information of a second cell. In particular, pointers to the system information are transmitted from the base station of the first cell to the mobile node, while the mobile node is located in the first cell. The MN processes said pointer, comprising e.g. information on the transmission format and timing of system information in the second cell. Using an acquisition gap assigned by the network, the MN changes the frequency to the second frequency so as to acquire the system information based on the previously received scheduling information. Thus, the acquisition time for important system information may be reduced, as no Master Information Block on the second frequency has to be processed first, in order to acquire the system information.

Various non-limiting embodiments disclosed herein relate generally to photochromic compounds, which may be thermally reversible or non-thermally reversible, and articles made therefrom. Other non-limiting embodiments relate to photochromic-dichroic compounds, which may be thermally reversible or non-thermally reversible, and articles made therefrom. For example, one non-limiting embodiment provides a thermally reversible, photochromic compound adapted to have at least a first state and a second state, wherein the thermally reversible, photochromic compound has an average absorption ratio greater than 2.3 in at least one state as determined according to CELL METHOD. Another non-limiting embodiment provides a photochromic compound comprising: (a) at least one photochromic group chosen from a pyran, an oxazine, and a fulgide; and (b) at least one lengthening agent L attached to the at least one photochromic group and represented by the formula —[S₁]_c-[Q₁-[S₂]_d]_d′-[Q₂-[S₃]_e]_e′-[Q₃-[S₄]_f]_f′—S₅—P, which is described herein.

Methods and materials for delivering biologically active molecules to cells in vitro or in vivo are provided. The methods and materials use carbon nanotubes or other hydrophobic particles, tubes and wires, functionalized with a linking group that is covalently bound to the nanotubes, or, alternatively, to the biologically active molecule, such as a protein. The biologically active molecule is preferably released from the nanotube when the complex has been taken up in an endosome.

Methods and systems are disclosed for temporarily modifying a macro-network neighbor list to enable a mobile station to hand off from a macro network to a femto cell. A method includes receiving a signal indicating that the mobile station detected coverage of a femto cell; after receiving the signal, adding the femto cell to the neighbor list, thereby enabling the mobile station to handoff to the femto cell; detecting that the mobile station completed handoff to the femto cell; and in response to detecting that the mobile station completed handoff to the femto cell, removing the femto cell from the neighbor list.

Methods and systems are provided for reducing interference across coverage cells using base stations interconnected by a packet network. A wireless device monitors the channel signal strength of its serving cell and its neighboring cell. The serving cell collects measurements from the wireless devices within its cell and informs its neighboring cell when one of the wireless device may be causing significant interference to the neighboring cell. The neighboring cell collects this information and executes a multi-user detection and cancellation algorithm on a subset of these interfering wireless devices.

Methods are disclosed to rapidly form and load cells and cell-derived vesicles. Loaded materials can include imaging agents, drugs and magnetic particles. Methods are also presented to additionally target the loaded cells or vesicles, leading to new forms of imaging, treatment, diagnosis, and detection by a large number of techniques. The preparation and use of reduced sized cells that retain subset characteristics of the parent cell are also described.

Methods and devices for isolating and diagnosing disease with a cell adhesion matrix system, mimicking a metastatic, cardiovascular or placental environment, are disclosed. The cell adhesion matrix facilitates the enrichment of target cells such as metastatic tumor cells, fetal cells and endothelial progenitor cells from a fluid sample such as blood for diagnostic and therapeutic applications in treating patients afflicted with disease, such as cancerous, cardiovascular and fetal diseases, as well as for research applications in molecular analysis of metastatic, and cardiovascular and fetal diseases. Blood test prototypes and methods for the cell enrichment and detection of circulating tumor and endothelial cells using multiplex molecular analysis are described herein. In addition, methods and compositions for determining host immunity to tumor in subjects with risk of cancer progression and methods for isolating an enriched fraction of fetal cells from pregnant females for prenatal diagnosis are also described herein.

Methods are described for mapping a pathway of differentiation of a population of embryonic cells which includes exposing the cells to an exogenous factor and measuring gene expression products that are characteristic of a particular cell type or lineage. Directing differentiation of human embryonic cells relies on dissociated embryoid bodies which are then exposed to one or more exogenous factors to enrich a culture for a particular cell type. The differentiated cells may be used for treating a medical condition in a human. Kits for determining differentiation pathways and screening exogenous factors for their utility in differentiation are provided.

Methods and devices for selectively removing from a subject a target cell, pathogen, or virus expressing a binding partner on its surface are presented. In one embodiment, the device contains an excorporeal circuit, which includes, at least, a magnetic filter comprising a magnet and a removable, magnetizable substrate capable of capturing magnetic nanomaterials; and a pump in fluid communication with the magnetic filter, wherein the pump moves fluid through the excorporeal circuit. The magnet is capable of generating a magnetic field sufficient to capture magnetic nanomaterials in the magnetic field. In a preferred embodiment, the target cells are cancer cells and/or cells infected with pathogenic agents. The devices may be designed for extracorporeal or in vivo uses. Functionalized superparamagnetic nanoparticles are either mixed ex vivo with a biological fluid from the patient or injected into the patient. Then the biological fluid, which includes the nanoparticles is transported to the magnetic filter to remove any nanoparticles that are complexed to the target cells, pathogens, or virus, and any free nanoparticles. Optionally, the functionalized nanoparticles contain and deliver a therapeutic agent. In one embodiment, the therapeutic agent is released when the nanoparticle binds to the target cells, pathogens, or virus.

Methods of identifying DNase I Hyper-Resistant Sites (DHRS), or in board sense, highly compact chromatin and characterizing the DNA methylation status of DMRs such as CpG islands and CpG island shores are provided. The methods are particularly useful for analysis of genomic DNA from low quantities of cells, for example, less than 1,000 cells, less than 100 cells, less than 10 cells, or even one cell, and can be used to generate chromatin and methylation profiles. The downstream analyses include in parallel massive sequencing, microarray, PCR and Sanger sequencing, hybridization and other platforms. These methods can be used to generate chromatin and DNA methylation profiles in drug development, diagnostics, and therapeutic applications are also provided.

Methods and compositions for tracking or monitoring uptake of siRNA by mammalian cells are provided. The methods and compositions may be used to monitoring the silencing activity of the internalized siRNA. The compositions contain an siRNA, an optically or magnetically detectable nanoparticle such as a quantum dot and, optionally, a transfection reagent. Cells are contacted with an siRNA and an optically or magnetically detectable nanoparticle, optionally in the presence of a transfection reagent. Detection of internalized nanoparticles is indicative of siRNA uptake. The invention allows analysis, identification, processing, etc., of cells that have efficiently taken up siRNA. In one embodiment, cells are sorted into at least two populations based on the amount of siRNA taken up.

Methods and compositions for amplifying the whole genome of a single cell are provided.

Methods for preparing a substrate for the arraying and immobilizing of biomolecules or cells are described. The methods include providing a solid support and depositing by a chemical plasma-mediated polymerization process a polymeric coating on the surface. The polymeric coating comprises at least one pendant functional group capable of attaching a biomolecule or a cell. A material of the polymeric coating is preferably selected in such a way that its surface properties enhance binding between the pendant group and the biomolecule or cell. A substrate for the immobilization of biomolecules and cells is also provided. The substrate comprises a solid support made of a polymeric material. The solid support has at least one first pendant functional group suitable for attaching a first biomolecule or a first cell on its surface. The substrate further comprises a polymeric coating deposited on the surface of the substrate. The polymeric coating increases the attachment of the first biomolecule or the first cell to the first functional group.

Methods for using vibration to harvest cells grown in 3D culture are provided. The methods entail the application of force to cells attached to a 3D matrix of sufficient amplitude, frequency, and duration to detach cells from the matrix and to flush the detached cells out of the matrix material. An apparatus for performing the methods of the invention as provided.

Methods and composition of induction of pluripotent stem cells are disclosed. For example, in certain aspects methods for generating induced pluripotent stem cells using reporter genes are described. Furthermore, the invention provides novel reprogramming vectors employing reporter genes.

Methods of forming capacitor-free DRAM cells include forming a field effect transistor by forming a first semiconductor wafer having a channel region protrusion extending therefrom and surrounding the channel region protrusion by an electrical isolation region. A portion of a backside of the first semiconductor wafer is then removed to define a semiconductor layer having a primary surface extending opposite the channel region protrusion and the electrical isolation region. A gate electrode is formed on the primary surface. The gate electrode extends opposite the channel region protrusion. The source and drain regions are formed in the semiconductor layer, on opposite sides of the gate electrode.