326 results about "Cell method" patented technology

Methods and materials for delivering biologically active molecules to cells in vitro or in vivo are provided. The methods and materials use carbon nanotubes or other hydrophobic particles, tubes and wires, functionalized with a linking group that is covalently bound to the nanotubes, or, alternatively, to the biologically active molecule, such as a protein. The biologically active molecule is preferably released from the nanotube when the complex has been taken up in an endosome.

Methods and systems are provided for reducing interference across coverage cells using base stations interconnected by a packet network. A wireless device monitors the channel signal strength of its serving cell and its neighboring cell. The serving cell collects measurements from the wireless devices within its cell and informs its neighboring cell when one of the wireless device may be causing significant interference to the neighboring cell. The neighboring cell collects this information and executes a multi-user detection and cancellation algorithm on a subset of these interfering wireless devices.

Methods of rapid distinction between growing cells and debris, which determine a time-lapse movie of specimen images, track features of each entity, and categorize each entity as growing cells or debris.

Methods and compositions for reducing viral genome amounts in a target cell are provided. In the subject methods, the activity of a miRNA is inhibited in a manner sufficient to reduce the amount of viral genome in the target cell, e.g., by introducing a miRNA inhibitory agent in the target cell. Also provided are pharmaceutical compositions, kits and systems for use in practicing the subject methods. The subject invention finds use in a variety of applications, including the treatment of subjects suffering from a viral mediated disease condition, e.g., an HCV mediated disease condition.

Methods are provided for diagnosis and prognosis of disease by analyzing expression of a set of genes obtained from single cell analysis. Classification allows optimization of treatment, and determination of whether on whether to proceed with a specific therapy, and how to optimize dose, choice of treatment, and the like. Single cell analysis also provides for the identification and development of therapies which target mutations and / or pathways in disease-state cells.

Methods, reagents, and kits are provided that permit flow cytometric determination of the phosphorylation status of retinoblastoma susceptibility gene protein (pRB) in individual cells. Methods are described that permit the hypophosphorylated, active, form of pRB to be measured either as an absolute quantity or as a proportion of total cellular pRB. Further described are methods that permit pRB phosphorylation status to be correlated with cell cycle phase and with protein components of the cell cycle. Screening of chemical compounds for antiproliferative and antineoplastic activity using the flow cytometric assays is demonstrated. Reagent kits that facilitate the subject methods are also provided.

Methods are provided to detect changes in cells without the use of detection labels.

Methods for using vibration to harvest cells grown in 3D culture are provided. The methods entail the application of force to cells attached to a 3D matrix of sufficient amplitude, frequency, and duration to detach cells from the matrix and to flush the detached cells out of the matrix material. An apparatus for performing the methods of the invention as provided.

Methods for performing surface-mediated protein delivery into living cells, and fabricating protein-transfected cell cluster arrays are provided. The method comprises providing a protein-containing mixture; depositing said protein-containing mixture onto a surface at defined locations; affixing the protein-containing mixture to the surface as microspots; and plating cells onto the surface in sufficient density and under conditions for the proteins to be delivered into the cells. The protein-containing mixture comprises any suitable amino acid sequence, including peptides, proteins, protein-domains, antibodies, or protein-nucleic acid conjugates, etc., with a carrier reagent. Protein-transfected cell arrays may be used for rapid and direct, screening of protein or enzymatic functions or any given intracellular protein interaction in the natural environment of a living cell, as well as for high-throughput screening of other biological and chemical analytes, which affect the functions of these proteins.

Methods and apparatus for high throughput wireless cells, capable of functioning as access points, are described. The wireless cells may be equipped with multiple radios to increase the amount of throughput available through a single wireless cell. Multiple antennas attached to the radios through RF switches may enable the wireless cell to service multiple clients simultaneously. The physical sectors of the antennas may overlapped to form virtual sectors that provide greater flexibility in client load management and in simultaneously servicing multiple clients. Attenuators may reduce interference from foreign wireless cells or clients. Systems and methods for assigning minimally interfering channels to either physical sectors or to radios to reduce interference between adjacent sectors and overlapping sectors are also disclosed. An antenna horn may also allow any antenna to be used as a directional antenna and may enable the antenna's angle of coverage to be adjusted. The use of multiple antennas on a client to reduce interferences is also described.

Methods of generating and expanding human hemangio-colony forming cells in vitro and methods of expanding and using such cells are disclosed. The methods permit the production of large numbers of hemangio-colony forming cells as well as derivative cells, such as hematopoietic and endothelial cells. The cells obtained by the methods disclosed may be used for a variety of research, clinical, and therapeutic applications.