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100 results about "Single-cell analysis" patented technology
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In the field of cellular biology, single-cell analysis is the study of genomics, transcriptomics, proteomics and metabolomics at the single cell level. Due to the heterogeneity seen in both eukaryotic and prokaryotic cell populations, analyzing a single cell makes it possible to discover mechanisms not seen when studying a bulk population of cells. Technologies such as fluorescence-activated cell sorting (FACS) or digital dielectrophoretic sorting (DEPArray), allow the precise isolation of selected single cells from complex samples, while high throughput single cell partitioning technologies, enable the simultaneous molecular analysis of hundreds or thousands single unsorted cells; this is particularly useful for the analysis of transcriptome variation in genotypically identical cells, allowing the definition of otherwise undetectable cell subtypes. The development of new technologies is increasing our ability to analyze the genome, and transcriptome, of single cells, as well as to quantify their proteome and metabolome. New developments in mass spectrometry techniques have become important analytical tools for proteomic and metabolomic analysis of single cells. In situ sequencing and fluorescence in situ hybridization (FISH) do not require that cells be isolated and are increasingly being used for analysis of tissues.
Computational techniques for three-dimensional reconstruction and multi-labeling of serially sectioned tissue
This document generally describes methods and systems for generating digital reconstructions of tissue from humans or other species. The method can, for example, include receiving, at a computing system, image data of a tissue sample, where one or more sections of the tissue sample are stained with hematoxylin and eosin (H&E), registering the image data to generate registered image data, identifying tissue subtypes based on application of a machine learning model to the registered image data, annotating the identified tissue subtypes to generate annotated image data, and determining a digital volume of the tissue sample in three dimensional (3D) space based on the annotated image data. The disclosed technology can provide for single-cell analysis and other analysis of tissue samples, such as early detection of cancer in human tissue samples.
Owner:JOHNS HOPKINS UNIVERSITY
Operation method of cell operation platform combining piezoelectric actuator and six-axis mechanical arm
The invention discloses an operation method of a cell operation platform combining a piezoelectric actuator and a six-axis mechanical arm, which integrates the large-stroke positioning capability of the mechanical arm and the nano-scale fine operation capability of the piezoelectric actuator, and combines the large-stroke positioning capability of the mechanical arm and the nano-scale fine operation capability of the piezoelectric actuator on the basis of macroscopic mechanical arm movement. The piezoelectric driving unit can accurately approach and control target cells in various postures, so that cell-level fine operation tasks such as cell surface treatment, single cell grabbing and cell injection are completed at high precision, and reliable technical support is provided for single cell analysis and operation.
Owner:GUANGDONG UNIV OF TECH
Centrifugal liquid drop generation micro-fluidic chip and digital PCR (polymerase chain reaction) detection method
PendingCN119662790Aeasy to handlegood repeatabilityBioreactor/fermenter combinationsBiological substance pretreatmentsEngineeringMgb probe
The invention relates to the technical field of microfluidics and digital PCR detection, and discloses a microfluidic chip for centrifugal droplet generation and a digital PCR detection method.The microfluidic chip comprises a sealed cavity formed by a bottom plate and a cover plate, a through hole is formed in the center of the chip and used for being fixed to centrifugal equipment, the core structure of the chip is a droplet generation component, and the droplet generation component is arranged in the cavity. Comprising a sample adding cavity, a micro-nozzle array and a liquid drop collecting cavity, a sample is driven by centrifugal force to generate nano-liter-scale'water-in-oil 'liquid drops through micro-nozzles, the chip is used for digital PCR reaction, each liquid drop serves as an independent reaction unit to improve detection sensitivity and precision, the chip is suitable for detection of low-abundance gene mutation, and by combining Taqman-MGB probe or other molecule detection technologies, the detection sensitivity and accuracy are improved. The chip can efficiently detect mutation types of EGFR genes, including EGFR-T790M mutation, has a wide clinical application prospect, provides a high-throughput and high-precision droplet generation method, and is suitable for application scenarios of digital PCR, single cell analysis and other microfluidic technologies.
Owner:THE AFFILIATED HOSPITAL OF TRADITIONAL CHINESE MEDICAL TO SOUTHWEST MEDICAL UNIV
Controllable cell lysis method in emulsion
PendingCN119685166ABioreactor/fermenter combinationsBiological substance pretreatmentsBiochemistryCell entrapment
The invention relates to the field of single cell analysis of droplet microfluidics, in particular to a controllable cell lysis method in an emulsion. The invention provides a method for cell lysis in a liquid drop, which comprises the following steps: 1) liquid drop single cell wrapping: wrapping a single cell into the liquid drop through a micro-fluidic chip in the presence of a first oil phase to obtain a first mixture containing the first oil phase and the liquid drop; and 2) cell lysis: replacing the first oil phase in the first mixture with a second oil phase containing a cell lysis solution, and incubating to realize cell lysis. For realizing cell lysis in the liquid drop, the second oil phase containing the cell lysis solution can realize cell lysis of the liquid drop outside the micro-fluidic chip, so that the operation difficulty is low, the cell lysis method is simple and efficient, low-cost, high-throughput and controllable cell lysis can be realized, and the applicability is wide.
Owner:SHANGHAI ADVANCED RES INST CHINESE ACADEMY OF SCI +1
Glass nanopore probe based on aptamer and application of glass nanopore probe in dopamine detection
PendingCN121613146AMicrobiological testing/measurementScanning probe microscopyAptamerComplementary deoxyribonucleic acid
The invention provides a glass nanopore probe based on an aptamer and application of the glass nanopore probe in dopamine detection, and belongs to the technical field of electrochemical sensing and scanning probe microscopy. The sensor comprises: a glass nanopore probe, the inner wall of which is modified with a gold layer; a cDNA (complementary deoxyribonucleic acid) single chain is fixed on the gold layer through a gold-sulfur bond; a part of the sequence of the dopamine aptamer is hybridized and combined with the cDNA single chain; wherein the combination of the dopamine and the dopamine aptamer causes the change of an ion current rectification signal of the glass nanopore probe, so that the detection of the dopamine is realized based on the change of the ion current rectification signal of the glass nanopore probe. The invention provides a valuable scheme for developing an aptamer nanopore scanning electrochemical sensor for single cell analysis and researching neurotransmitter-related diseases.
Owner:TIANJIN UNIV
Micro-fluidic chip for chemiluminescence imaging detection of single-cell secretions and use method of micro-fluidic chip
The invention relates to a micro-fluidic chip for chemiluminescence imaging detection of single-cell secretions and a use method of the micro-fluidic chip. The micro-fluidic chip is composed of a single cell analysis layer and a glass substrate layer. The single cell analysis layer is prepared from polydimethylsiloxane and is provided with a capture channel, a sample introduction channel and a lantern-shaped microstructure array, the lantern-shaped microstructure comprises a small-size cell capture trap and a large-size chemiluminescence detection chamber, the small-size cell capture trap is connected with the capture channel, and the large-size chemiluminescence detection chamber is connected with the sample introduction channel. After a mixed solution of cells and a detection reagent is introduced into the capture channel, single cell capture is realized on the capture trap due to flow resistance difference; then, introducing air into the capture channel and the sample introduction channel to form a gas-in-liquid microcavity, and carrying out single cell isolation and incubation reaction; finally, the signal switch molecules and the substrate liquid are sequentially injected into the chemiluminescence detection chamber through the sample injection channel, and quantitative detection is conducted on the single-cell secretions according to chemiluminescence imaging signals.
Owner:NANJING UNIV
Gene testing method for t-cell receptor (TCR) or b-cell receptor (BCR) and gene testing kit
PendingJP2025187393ANucleotide librariesMicrobiological testing/measurementMolecular identificationCellular receptor
To provide a gene testing method and a gene testing kit for analyzing a variable region of a TCR or a BCR in single-cell analysis.SOLUTION: A method including a step of capturing mRNA eluted from a single cell using an RT probe immobilized on a solid-phase surface and including a common sequence, a cell identification barcode, a first molecular identification barcode, and an oligo(dT) sequence, a step of performing a reverse transcription reaction in a reverse transcription reaction solution containing a TSO using the mRNA as a template to generate cDNA having a sequence added to a 3' end, a step of degrading the mRNA, a step in which a primer for inserting a second molecular identification barcode binds to a constant region adjacent to a variable region of a TCR or a BCR and includes the second molecular identification barcode, a step of ligating cDNA extended from the primer with a ligase, a step of amplifying a fragment including the second molecular identification barcode and the variable region of the TCR or the BCR using a primer, a step of performing sequence analysis, and a step of associating sequences.SELECTED DRAWING: Figure 2
Owner:HITACHI LTD
Rapid programmable generation device and method for cascade droplet array
The invention discloses a rapid programmable generation device and method for a cascade droplet array. The device comprises a hydrophobic fan-shaped substrate, a scraper blade, a radially arranged hydrophilic stack point array and a driving device. Driving the mother liquid drops to move along the surface of the hydrophobic substrate through the rotation of the scraper, and dividing the mother liquid drops into micro-droplets residing in a hydrophilic area by utilizing the surface tension difference between the hydrophilic stack point and the hydrophobic substrate; the liquid drop volume is dynamically controlled by adjusting the rotating speed of the scraper blade or the diameter gradient distribution of the stack points, and the gradient liquid drop array is generated through single operation. The device does not need an external pump valve or a precise sealing device, has the advantages of open type passive operation, low cost, high flux, high stability and the like, is suitable for scenes of drug screening, single cell analysis, instant detection and the like, and remarkably improves the flexibility of micro-droplet generation and the experiment efficiency.
Owner:NORTHWESTERN POLYTECHNICAL UNIV
Microorganism detection device based on single cell analysis
PendingCN120272309ABioreactor/fermenter combinationsBiological substance pretreatmentsBiotechnologyMicroorganism
The invention discloses a microbiological detection device based on single cell analysis, which comprises an incubator, an assembly frame is arranged on the upper side of the incubator, a driving part is arranged on the lower side of the assembly frame, the driving end of the driving part penetrates through the assembly frame and is connected with a turntable, and a support frame is arranged on the upper side of the assembly frame. According to the technical scheme, the incubator and the detection probe of the detection device are combined, the incubator and the detection probe of the detection device are assembled in a vertical arrangement mode, the distance between the incubator and the detection probe of the detection device is short, and then detection operation can be directly carried out after microorganism culture is finished; the condition of pollution in the sample transfer process can be reduced, and the detection quality is improved.
Owner:CHONGQING IND POLYTECHNIC COLLEGE +2
A method for pico-coulomb level single bacterium surface charge imaging and application
This invention belongs to the field of microbial electrochemistry and single-cell analysis technology, and specifically relates to a femtocoulomb-level single-bacterial surface charge imaging method and its application. This technology is based on electrochemiluminescence microscopy, utilizing the spontaneous enrichment of cationic ECL luminescent molecules by the electrostatic field of the negative charge on the bacterial surface, thereby non-destructively enhancing its ECL signal. By establishing a quantitative relationship between the ECL intensity enhancement ratio and the number of adsorbed molecules, and combining this with COMSOL simulation to determine the thickness of the luminescent layer, it achieves for the first time high-throughput, high spatiotemporal resolution dynamic quantitative measurement of femtocoulomb-level charges on the surface of a single living bacterium. The invented technology has high sensitivity, high resolution, and good biocompatibility, and can be used to reveal bacterial metabolic-charge regulation mechanisms, analyze synergistic electron transport pathways, and screen highly electroactive bacterial subpopulations in situ, providing a key tool for microbial electrochemical research.
Owner:NANJING UNIV
Liquid biopsy for diagnosis of early osteoarthritis
PendingUS20250316378A1Mechanical/radiation/invasive therapiesDrug and medicationsDiseaseBlood specimen
Compositions and methods are provided for determining the presence of early-stage osteoarthritis (OA) in an individual by single cell profiling of a blood sample. Through use of machine learning, it is shown that immune cell features associated with OA are present and detectable in the early stages of OA and can be utilized for early detection of the disease.
Owner:THE BOARD OF TRUSTEES OF THE LELAND STANFORD JUNIOR UNIV
A single-cell online extension lysis mass spectrometry flow analysis method
ActiveCN116429667BMaterial analysis by electric/magnetic meansAgainst vector-borne diseasesMetaboliteMass spectrometry detector
The application discloses a single-cell online prolonging lysis mass spectrometry flow analysis method. A cell suspension sample is injected into a separation capillary, a front section of the capillary is spirally wound to realize single dispersion of the cells, and a part of a rear section of the capillary is located in an ultrasonic device to realize online ultrasonic lysis, localized single-cell lysis contents are obtained, the content solution gradually enters a non-contact electrospray needle to realize ionization, and finally enters a mass spectrometry detector for detection. Meanwhile, the analysis coverage of weakly polar compounds can be improved by combining a plasma generation device. The method can realize single-cell analysis with high throughput and high metabolite coverage, and by means of a prolonged single-cell detection window, the collection of metabolite primary mass-to-charge ratio information and the identification of secondary structures under high resolution can be realized, including qualitative and quantitative analysis of positional isomers, so that the method has wide application prospects in the fields of cell identification, cell typing, differential substance research, tumor diagnosis and biomarker mining.
Owner:PEKING UNIV
RNA detection and amplification method for single cell analysis on fixed cells
The disclosure includes systems, methods, compositions, and kits for determining the spatial position and / or copy number of a nucleic acid target in a fixed sample. In some embodiments, detection oligonucleotides comprising a coupling sequence, a probe sequence configured to hybridize to a nucleic acid target, and / or a predetermined spatial label are provided. Methods can include contacting each of two or more spatial positions of a sample comprising a copy of a nucleic acid target with the probe oligonucleotide. Methods can include in situ extending a probe oligonucleotide hybridized to a copy of a nucleic acid target to produce more than one extended probe oligonucleotide. In some embodiments, a method may include barcoding more than one extended probe oligonucleotide using more than one oligonucleotide barcode to generate more than one barcoded probe oligonucleotide.
Owner:BECTON DICKINSON & CO
Single cell mapping and transcriptome analysis
Methods of tagging cells with unique oligonucleotide “zipcode” constructs are provided. By these methods and associated compositions, cells in a multicellular structure such as a tissue section can be tagged with a construct, the unique composition of which is associated with the cells position in the multicellular structure. Subsequently, the multicellular structure can be dissociated into single cells and a single cell transcriptome analysis performed, as well as other types of single cell analyses. By preserving positional information in the analyzed single cells, biological processes within the tissue can be mapped. By these methods, the effects of the local environment surrounding a cell on its state and various functions can be elucidated, and intra-tissue processes can be mapped and observed. Likewise, coordinated actions by multiple cells within a tissue can be mapped and tracked over time.
Owner:RGT UNIV OF CALIFORNIA
A transposase-based 5mCpG high-throughput detection method TnM-seq, transposase TnM and single-cell multi-omics application
The application provides a 5mCpG high-throughput detection method TnM-seq based on TnM transposase, which adopts transposase TnM including an MBD domain to detect 5mCpG in situ, and comprises the following steps: after pretreatment of cells to be detected, the cells are incubated with transposase TnM; DNA in the target sample after transposition is recovered and purified; the purified DNA is subjected to PCR library construction; and the library construction product is subjected to high-throughput sequencing. The new transposase TnM newly designed and synthesized is used, combined with high-throughput sequencing, to realize convenient, fast, low-cost and high-sensitivity detection of 5mCpG in a sample. Based on the transposase TnM, a multi-omics technology HAM-seq for simultaneously detecting histone modification H3K9me3, chromatin accessibility and DNA methylation modification 5mCpG in the same sample is further developed, and after being combined with a commercial single-cell microfluidic platform, an epigenome and gene expression four-omics technology EpiX-seq for single-cell analysis in a complex tissue is obtained.
Owner:ZHEJIANG UNIV
Single-cell profiling of phagocytosis using microscopy
The invention relates to methods of evaluating phagocytosis at a single-cell level by placing one or more phagocytes and one or more particles on a surface and visualizing the phagocytosis of the particles by the phagocytes. The invention also relates to systems for evaluating phagocytosis of a particle by a phagocyte at a single-cell level.
Owner:UNIV HOUSTON SYST
Method for automatically detecting abnormal cells in urine exfoliated cells
PendingCN120629142APreparing sample for investigationMaterial analysis by optical meansTest sampleAbnormal cells
The invention provides a method for automatically detecting abnormal cells in urine cast-off cells, which comprises the following steps of: training an abnormal cell frame selection recognition algorithm, and generating an abnormal cell frame selection recognition model; according to the abnormal cell frame selection recognition model, abnormal cell frame selection recognition of a to-be-tested sample is achieved. The method is suitable for early preliminary diagnosis screening and postoperative reexamination of bladder urothelium carcinoma, has the advantages of early noninvasive detection, convenience in analysis and report, simplicity and standardization in operation, suitability for automatic analysis of instruments and the like, and not only can eliminate non-cell impurities in a complex background, but also can effectively separate overlapped cells, so that the detection accuracy is improved. And a foundation is laid for subsequent single cell analysis.
Owner:FIRST AFFILIATED HOSPITAL OF DALIAN MEDICAL UNIV
Microfluidic chip (for use in single cell sorting based on label-free or fluorescence detection)
1. Name of the Design Product: Microfluidic Chip (for Single-Cell Sorting Based on Label-Free or Fluorescent Detection). 2. Use of the Design Product: Microfluidic chip, for biological particles such as cells, implementing applications such as single-cell analysis, manipulation, and sorting based on label-free or labeled detection methods. 3. Design Key Points of the Design Product: Lies in the shape. 4. Picture or Photograph Most Illustrating the Design Key Points: Perspective view. 5. Since it is already shown in other views, the right view and the bottom view are omitted. 6. Part A is transparent or semi-transparent.
Owner:DALIAN HUAWEI LIFE TECH CO LTD
A ratiometric bipolar electrode electrochemiluminescence biosensor based on quasi-homogeneous reaction and its application
ActiveCN116818865BMaterial electrochemical variablesAgainst vector-borne diseasesMagnetite NanoparticlesElectrochemiluminescence
The present invention discloses a ratiometric bipolar electrode electrochemiluminescence biosensor based on quasi-homogeneous reaction and its application. 2+ Using the DNA / TPrA system as a dual-anode signal source, a novel ratiometric bipolar electrode electrochemiluminescence biosensor for miRNA detection and analysis was constructed. By combining the ligase chain reaction with CRISPR / Cas technology and integrating gold nanoparticle-modified Fe3O4 magnetic nanoparticles enriched with ferrocene nucleic acid signal probes (DNA-Fc / Au@Fe3O4 NPs) as a multifunctional reaction interface, the sensor effectively modulates the signal changes of the two ECL reaction systems. Ultrasensitive detection of miRNA-222 is achieved through the ratio of the two signals. The constructed sensor exhibits exceptional performance for miRNA-222 detection and is expected to be applied as a novel detection method in early clinical diagnosis and single-cell analysis.
Owner:FUJIAN MEDICAL UNIV
A microwell chip and single cell analysis method
ActiveCN117019248BBioreactor/fermenter combinationsBiological substance pretreatmentsEpigenomeSingle cell suspension
The present invention discloses a microporous chip and a single-cell analysis method. The microporous chip is provided with a plurality of double-pass penetrating micropores, and the micropores are used to load a single-cell suspension or a single-cell nuclear suspension of a sample to be tested and microbeads with nucleic acid molecules of known base sequences. The single-cell analysis method comprises the following steps: S1, mixing a single-cell suspension or a single-cell nuclear suspension of a tissue sample to be tested with microbeads with nucleic acid molecules of known base sequences and loading the mixture into the micropores of the microporous chip; S2, polymerizing and amplifying nucleic acid molecules on the microporous chip in S1 to construct a single-cell sequencing library. The microporous chip and the single-cell analysis method of the present invention can be applied to high-throughput single-cell multi-omics sequencing, and can obtain specific omics information such as full-length transcriptomes, epigenomes, and proteomes of hundreds of thousands to millions of single cells in batches at one time.
Owner:HANGZHOU YUEZHEN BIOTECHNOLOGY CO LTD
Thresholding techniques for supplementary nucleotide tag assignment in single-cell analysis
The present disclosure provides systems and methods for making sequencing libraries that are useful for quantitatively analyzing nucleic acids in a sample. In certain implementations, assigning of cell-associated features within single-cell data may be practiced as part of sample processing and / or as a standalone analysis tool. Cells determined to be positive for a respective class or classes of gRNA may then be further processed using other analysis operations described herein.
Owner:ILLUMINA INC
Cell spectral imaging device and method
The invention relates to a spectral imaging technology, and particularly provides a cell spectral imaging device and method, the imaging device comprises a light source and a detector; the adjusting unit reflects emergent light of the light source, so that the reflected light sweeps cells flowing in the channel in two dimensions, and the cells are stained with fluorescein; the light splitting unit splits fluorescence emitted by the excited cell, and the detector outputs three-dimensional spectrum data related to the position and wavelength; the processing unit is used for carrying out spectrum unfolding on the three-dimensional spectrum data to obtain a multi-channel image; and the controller is used for driving the adjusting unit and the detector to sample and trigger. The method has the advantages of high resolution and the like, and is applied to single cell analysis.
Owner:杭州谱康医学科技有限公司 +1
A liver cancer prognosis marker and application thereof
The application discloses a liver cancer prognosis marker and application thereof, and the liver cancer prognosis marker comprises TXNRD1, HAO1, C1S, OPTN and SNX6. The application provides a group of liver cancer prognosis markers, and a liver cancer patient prognosis prediction model is constructed based on the liver cancer prognosis markers. The liver cancer prognosis markers are obtained through comprehensive gene expression levels, single cell analysis and spatial transcriptome analysis, and the diversity of the data set and the large sample quantity improve the reliability and universality of the liver cancer prognosis markers and the liver cancer patient prognosis prediction model.
Owner:SHENZHEN PEOPLES HOSPITAL
Use of ndufb4 as a target in the preparation of a drug for treating bladder cancer
The application discloses application of NDUFB4 as a target point in preparation of a drug for treating bladder cancer. Single cell analysis results show that NDUFB4 is highly enriched in bladder cancer epithelial cells, and can be used as a key coordinator to participate in the regulation of multiple carcinogenic signal pathways. Through shRNA-mediated gene silencing and CRISPR / Cas9-mediated gene knockout experiments, it is found that NDUFB4 deletion can significantly inhibit the proliferation, migration and invasion ability of bladder cancer cells, and cause a serious bioenergy crisis, and activate the endogenous apoptosis pathway. In a subcutaneous xenotransplant tumor model, it is further verified that NDUFB4 silencing can significantly inhibit tumor growth. The application discloses that NDUFB4 is a key coordinator between mitochondrial high-functionality and carcinogenic signal pathways, and it is suggested that NDUFB4 can become a potential target for bladder cancer treatment.
Owner:CHANGZHOU NO 2 PEOPLES HOSPITAL
Systems, compositions, and methods for single cell analysis
Provided herein are systems, compositions, and methods for single cell analysis. In particular, provided herein are systems, compositions, and methods for scalable, high-throughput isolation and sequencing of nucleic acids from rare and / or fragile single cells.
Owner:FLUID DISCOVERY
Non-enzymatic dissociation of FFPE tissue and generation of single cells with intact cell surface markers
The present disclosure is directed to a non-enzymatic dissociation method which facilitates the dissociation of one or more formalin-fixed paraffin-embedded tissue samples into dissociated single cells. The present disclosure is also directed to methods of single cell analysis, i.e., methods of analyzing and / or measuring target components (e.g., biomolecules such as, but not limited to, polypeptides, polynucleotides, small molecules, and the like) on or in cells non-enzymatically dissociated from one or more FFPE tissue samples.
Owner:VENTANA MEDICAL SYSTEMS INC
Methods, compositions and systems for assessing quality of biological samples
InactiveCN120129825AMicrobiological testing/measurementPreparing sample for investigationAnalyteLight signal
In some aspects, the present disclosure relates to methods and compositions for quality control (e.g., assessing the immobilization level of a biological sample) and / or optimizing the detection of an analyte in an immobilized sample. In some embodiments, the method can be used to treat an immobilized biological sample, the method comprising contacting the immobilized biological sample with a nucleic acid stain and / or an actin stain; detecting a light signal associated with the nucleic acid stain and / or a light signal associated with the actin stain; the detected light signal is compared to one or more references to determine the quality of the biological sample. Single cell analysis, spatial array-based analysis, or in situ analysis may be performed using the treated biological sample.
Owner:10X GENOMICS INC
Genetic test method and genetic test kit for t cell receptor (TCR) or b cell receptor (BCR)
PCT designated stageWO2025258291A1Nucleotide librariesMicrobiological testing/measurementCellular receptorB-cell receptor
The present invention provides a genetic test method and a genetic test kit for immune cells in blood. Specifically, the present invention provides a genetic test method and a genetic test kit for analyzing a variable region of a T cell receptor (TCR) or a B cell receptor (BCR) in a single cell analysis technique.
Owner:HITACHI LTD
Liquid drop micro-fluidic chip based on micro-filtration array
PendingCN121103443ABioreactor/fermenter combinationsBiological substance pretreatmentsEngineeringBiochemistry
The invention provides a liquid drop micro-fluidic chip based on a micro-filtration array. The micro-filtration array structure is highly integrated in the water phase sample introduction area of the chip, synchronous filtration of cell suspension in the inflow process is achieved, newly formed cell blocks or fragments can be effectively intercepted and prevented from entering the liquid drop generation area, and therefore the liquid drop wrapping quality is guaranteed from the source. In an actual test, the droplet micro-fluidic chip has an efficient filtering capability, supports the droplet generation frequency of about 1000 Hz, has been verified in a 293T cell suspension system, has good stability and repeatability, and can be widely applied to single cell sequencing, cell screening and other high-throughput single cell analysis scenes.
Owner:MOBIDROP (ZHEJIANG) CO LTD +1
Single-cell analysis system and method based on precise typing of lung cancer immune microenvironment and treatment prediction
This invention relates to the fields of bioinformatics and precision oncology medicine, specifically disclosing a single-cell analysis system and method based on precise typing and treatment prediction of the lung cancer immune microenvironment. The system includes a data preprocessing and quality control module, a lung cancer-specific cell annotation module, an immune microenvironment typing module, a clinical efficacy prediction module, and a visualization report generation module. These modules form a complete technical chain from raw data to clinical efficacy prediction. Furthermore, this invention provides a scheme for precise typing and treatment prediction of the lung cancer immune microenvironment matching this system. This invention can take single-cell transcriptome data (which can integrate spatial transcriptome data) from lung cancer patients as input, process it through a series of specific computational modules, and finally output immune microenvironment typing results and treatment response prediction results with clear clinical guidance significance, solving the problems of unclear typing, fragmented processes, and insufficient clinical translation capabilities in existing technologies.
Owner:WEST CHINA HOSPITAL SICHUAN UNIV