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125 results about "Single-cell analysis" patented technology
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In the field of cellular biology, single-cell analysis is the study of genomics, transcriptomics, proteomics and metabolomics at the single cell level. Due to the heterogeneity seen in both eukaryotic and prokaryotic cell populations, analyzing a single cell makes it possible to discover mechanisms not seen when studying a bulk population of cells. Technologies such as fluorescence-activated cell sorting (FACS) or digital dielectrophoretic sorting (DEPArray), allow the precise isolation of selected single cells from complex samples, while high throughput single cell partitioning technologies, enable the simultaneous molecular analysis of hundreds or thousands single unsorted cells; this is particularly useful for the analysis of transcriptome variation in genotypically identical cells, allowing the definition of otherwise undetectable cell subtypes. The development of new technologies is increasing our ability to analyze the genome, and transcriptome, of single cells, as well as to quantify their proteome and metabolome. New developments in mass spectrometry techniques have become important analytical tools for proteomic and metabolomic analysis of single cells. In situ sequencing and fluorescence in situ hybridization (FISH) do not require that cells be isolated and are increasingly being used for analysis of tissues.
Computational techniques for three-dimensional reconstruction and multi-labeling of serially sectioned tissue
This document generally describes methods and systems for generating digital reconstructions of tissue from humans or other species. The method can, for example, include receiving, at a computing system, image data of a tissue sample, where one or more sections of the tissue sample are stained with hematoxylin and eosin (H&E), registering the image data to generate registered image data, identifying tissue subtypes based on application of a machine learning model to the registered image data, annotating the identified tissue subtypes to generate annotated image data, and determining a digital volume of the tissue sample in three dimensional (3D) space based on the annotated image data. The disclosed technology can provide for single-cell analysis and other analysis of tissue samples, such as early detection of cancer in human tissue samples.
Owner:JOHNS HOPKINS UNIVERSITY
Operation method of cell operation platform combining piezoelectric actuator and six-axis mechanical arm
The invention discloses an operation method of a cell operation platform combining a piezoelectric actuator and a six-axis mechanical arm, which integrates the large-stroke positioning capability of the mechanical arm and the nano-scale fine operation capability of the piezoelectric actuator, and combines the large-stroke positioning capability of the mechanical arm and the nano-scale fine operation capability of the piezoelectric actuator on the basis of macroscopic mechanical arm movement. The piezoelectric driving unit can accurately approach and control target cells in various postures, so that cell-level fine operation tasks such as cell surface treatment, single cell grabbing and cell injection are completed at high precision, and reliable technical support is provided for single cell analysis and operation.
Owner:GUANGDONG UNIV OF TECH
Single-cell drug causal gene discovery method and system based on anti-transfer learning
ActiveCN119446281Aimprove accuracyavoid drug resistanceBiostatisticsBiological modelsData setPharmacy medicine
The invention discloses a single-cell drug causal gene discovery method and system based on adversarial transfer learning, and relates to the technical field of drug sensitivity prediction.The method comprises the steps that a source domain data set and a target domain data set are obtained, an adversarial transfer model is constructed, and the adversarial transfer model is trained and optimized according to the source domain data set and the target domain data set; obtaining a single-cell drug sensitivity prediction result; modeling single-cell gene expression data, combining a k-adjacent matching algorithm and a single-cell drug sensitivity prediction result to obtain an anti-fact value, and further calculating a hybrid effect and a difference effect; and calculating the causal effect based on the hybrid effect and the difference effect, and screening out the causal gene of the single-cell drug. On one hand, the key problem of lack of drug sensitivity labeling data in single cell analysis is solved, on the other hand, the causal gene is successfully found, and an important clue is provided for deep understanding of a drug action mechanism.
Owner:SHANDONG UNIV
Liquid drop positioning printing method and single cell analysis platform
PendingCN119101597AImprove efficiencyHigh precisionBioreactor/fermenter combinationsBiological substance pretreatmentsGenomicsOptical Module
The invention provides a liquid drop positioning printing method and a single cell analysis platform, and the method is used for calibrating the position of a nozzle before liquid drop printing, and comprises the following steps: pre-printing a plurality of liquid drops on a base material by using at least two specified nozzles, and obtaining the position of a pre-printed liquid drop point; and correcting the theoretical value coordinate of the nozzle position based on the drop point position to obtain a correction value coordinate of the nozzle position. According to different types of printing heads, high-efficiency and high-precision liquid drop arrangement operation can be completed under different liquid and different printing conditions, so that high-throughput and high-accuracy single cell operation can be completed by utilizing the single cell sorting device. The single cell analysis platform comprises a control assembly, a single cell sorting and printing device, a dielectric wetting chip and an optical module, and can realize pretreatment of single cell capture, fixation, dyeing and the like on a micro platform at a micron-to-millimeter level, so as to realize high-throughput analysis of cell morphology, genomics, transcriptomics and proteomics.
Owner:SHANGHAI AUREFLUIDICS TECH CO LTD
Centrifugal liquid drop generation micro-fluidic chip and digital PCR (polymerase chain reaction) detection method
PendingCN119662790Aeasy to handlegood repeatabilityBioreactor/fermenter combinationsBiological substance pretreatmentsEngineeringMgb probe
The invention relates to the technical field of microfluidics and digital PCR detection, and discloses a microfluidic chip for centrifugal droplet generation and a digital PCR detection method.The microfluidic chip comprises a sealed cavity formed by a bottom plate and a cover plate, a through hole is formed in the center of the chip and used for being fixed to centrifugal equipment, the core structure of the chip is a droplet generation component, and the droplet generation component is arranged in the cavity. Comprising a sample adding cavity, a micro-nozzle array and a liquid drop collecting cavity, a sample is driven by centrifugal force to generate nano-liter-scale'water-in-oil 'liquid drops through micro-nozzles, the chip is used for digital PCR reaction, each liquid drop serves as an independent reaction unit to improve detection sensitivity and precision, the chip is suitable for detection of low-abundance gene mutation, and by combining Taqman-MGB probe or other molecule detection technologies, the detection sensitivity and accuracy are improved. The chip can efficiently detect mutation types of EGFR genes, including EGFR-T790M mutation, has a wide clinical application prospect, provides a high-throughput and high-precision droplet generation method, and is suitable for application scenarios of digital PCR, single cell analysis and other microfluidic technologies.
Owner:THE AFFILIATED HOSPITAL OF TRADITIONAL CHINESE MEDICAL TO SOUTHWEST MEDICAL UNIV
Controllable cell lysis method in emulsion
PendingCN119685166ABioreactor/fermenter combinationsBiological substance pretreatmentsBiochemistryCell entrapment
The invention relates to the field of single cell analysis of droplet microfluidics, in particular to a controllable cell lysis method in an emulsion. The invention provides a method for cell lysis in a liquid drop, which comprises the following steps: 1) liquid drop single cell wrapping: wrapping a single cell into the liquid drop through a micro-fluidic chip in the presence of a first oil phase to obtain a first mixture containing the first oil phase and the liquid drop; and 2) cell lysis: replacing the first oil phase in the first mixture with a second oil phase containing a cell lysis solution, and incubating to realize cell lysis. For realizing cell lysis in the liquid drop, the second oil phase containing the cell lysis solution can realize cell lysis of the liquid drop outside the micro-fluidic chip, so that the operation difficulty is low, the cell lysis method is simple and efficient, low-cost, high-throughput and controllable cell lysis can be realized, and the applicability is wide.
Owner:SHANGHAI ADVANCED RES INST CHINESE ACADEMY OF SCI +1
Probe-based device-free single-cell RNA profiling
Provided are methods and compositions for in situ detection of RNA in cells that does not require either the use of reverse transcriptase nor use of droplets. The methods can comprise annealing, in fixed and permeabilized cells, a pair of polynucleotide probes to adjacent sequences in a target RNA, which are subsequently ligated. A cell-specific barcode sequence can be subsequently synthesized in the cell using split-pool rounds to add barcode sequences to the ligated probe pair sequences in the cells, wherein an effect of multiple rounds of the split pooling is that ligated probe pair sequences in different cells have unique barcodes that are cell-specific.
Owner:CZ BIOHUB SF LLC +1
Glass nanopore probe based on aptamer and application of glass nanopore probe in dopamine detection
PendingCN121613146AMicrobiological testing/measurementScanning probe microscopyAptamerComplementary deoxyribonucleic acid
The invention provides a glass nanopore probe based on an aptamer and application of the glass nanopore probe in dopamine detection, and belongs to the technical field of electrochemical sensing and scanning probe microscopy. The sensor comprises: a glass nanopore probe, the inner wall of which is modified with a gold layer; a cDNA (complementary deoxyribonucleic acid) single chain is fixed on the gold layer through a gold-sulfur bond; a part of the sequence of the dopamine aptamer is hybridized and combined with the cDNA single chain; wherein the combination of the dopamine and the dopamine aptamer causes the change of an ion current rectification signal of the glass nanopore probe, so that the detection of the dopamine is realized based on the change of the ion current rectification signal of the glass nanopore probe. The invention provides a valuable scheme for developing an aptamer nanopore scanning electrochemical sensor for single cell analysis and researching neurotransmitter-related diseases.
Owner:TIANJIN UNIV
Analytical device for performing single cell analysis on tumor using artificial intelligence, and method using the same
Disclosed is a single cell analysis device for analyzing a tumor using artificial intelligence, the device including, a memory, and a processor, and the processor is configured to acquire single cell data on a tumor of a particular patient through the communication part; pre-process the single cell data on the tumor through a data pre-processing module; classify a type of the single cell based on the data pre-processed by a cell type defining module; predict a phenotype derivable from RNA expression of the tumor of the particular patient through a single-cell state predicting module materialize heterogeneity of the tumor of the particular patient through the tumor analyzing module; and report and provide an analysis result of the tumor of the particular patient through an analysis result generating module.
Owner:WITTGEN BIOTECHNOLOGIES INC
Micro-fluidic chip for chemiluminescence imaging detection of single-cell secretions and use method of micro-fluidic chip
The invention relates to a micro-fluidic chip for chemiluminescence imaging detection of single-cell secretions and a use method of the micro-fluidic chip. The micro-fluidic chip is composed of a single cell analysis layer and a glass substrate layer. The single cell analysis layer is prepared from polydimethylsiloxane and is provided with a capture channel, a sample introduction channel and a lantern-shaped microstructure array, the lantern-shaped microstructure comprises a small-size cell capture trap and a large-size chemiluminescence detection chamber, the small-size cell capture trap is connected with the capture channel, and the large-size chemiluminescence detection chamber is connected with the sample introduction channel. After a mixed solution of cells and a detection reagent is introduced into the capture channel, single cell capture is realized on the capture trap due to flow resistance difference; then, introducing air into the capture channel and the sample introduction channel to form a gas-in-liquid microcavity, and carrying out single cell isolation and incubation reaction; finally, the signal switch molecules and the substrate liquid are sequentially injected into the chemiluminescence detection chamber through the sample injection channel, and quantitative detection is conducted on the single-cell secretions according to chemiluminescence imaging signals.
Owner:NANJING UNIV
Gene testing method for t-cell receptor (TCR) or b-cell receptor (BCR) and gene testing kit
PendingJP2025187393ANucleotide librariesMicrobiological testing/measurementMolecular identificationCellular receptor
To provide a gene testing method and a gene testing kit for analyzing a variable region of a TCR or a BCR in single-cell analysis.SOLUTION: A method including a step of capturing mRNA eluted from a single cell using an RT probe immobilized on a solid-phase surface and including a common sequence, a cell identification barcode, a first molecular identification barcode, and an oligo(dT) sequence, a step of performing a reverse transcription reaction in a reverse transcription reaction solution containing a TSO using the mRNA as a template to generate cDNA having a sequence added to a 3' end, a step of degrading the mRNA, a step in which a primer for inserting a second molecular identification barcode binds to a constant region adjacent to a variable region of a TCR or a BCR and includes the second molecular identification barcode, a step of ligating cDNA extended from the primer with a ligase, a step of amplifying a fragment including the second molecular identification barcode and the variable region of the TCR or the BCR using a primer, a step of performing sequence analysis, and a step of associating sequences.SELECTED DRAWING: Figure 2
Owner:HITACHI LTD
Pre-enrichment for single-cell analysis for detecting measurements of residual disease and analyzing circulating tumor cells
Disclosed herein are methods for analyzing rare disease cells of a plurality of subjects. The methods include obtaining a plurality of samples from the plurality of subjects; for each sample in the plurality of samples, enriching the sample to obtain rare disease cells; pooling the obtained rare disease cells across the plurality of samples; providing the pooled rare disease cells for single-cell analysis to generate amplicons derived from analytes of the pooled rare disease cells; sequencing the amplicons derived from analytes of the pooled rare disease cells; clustering the rare disease cells across the plurality of samples using the sequenced amplicons; and de-multiplexing the rare disease cells by assigning clusters of rare disease cells to individual subjects of the plurality of subjects.
Owner:MISSION BIO INC
Rapid programmable generation device and method for cascade droplet array
The invention discloses a rapid programmable generation device and method for a cascade droplet array. The device comprises a hydrophobic fan-shaped substrate, a scraper blade, a radially arranged hydrophilic stack point array and a driving device. Driving the mother liquid drops to move along the surface of the hydrophobic substrate through the rotation of the scraper, and dividing the mother liquid drops into micro-droplets residing in a hydrophilic area by utilizing the surface tension difference between the hydrophilic stack point and the hydrophobic substrate; the liquid drop volume is dynamically controlled by adjusting the rotating speed of the scraper blade or the diameter gradient distribution of the stack points, and the gradient liquid drop array is generated through single operation. The device does not need an external pump valve or a precise sealing device, has the advantages of open type passive operation, low cost, high flux, high stability and the like, is suitable for scenes of drug screening, single cell analysis, instant detection and the like, and remarkably improves the flexibility of micro-droplet generation and the experiment efficiency.
Owner:NORTHWESTERN POLYTECHNICAL UNIV
Microorganism detection device based on single cell analysis
PendingCN120272309ABioreactor/fermenter combinationsBiological substance pretreatmentsBiotechnologyMicroorganism
The invention discloses a microbiological detection device based on single cell analysis, which comprises an incubator, an assembly frame is arranged on the upper side of the incubator, a driving part is arranged on the lower side of the assembly frame, the driving end of the driving part penetrates through the assembly frame and is connected with a turntable, and a support frame is arranged on the upper side of the assembly frame. According to the technical scheme, the incubator and the detection probe of the detection device are combined, the incubator and the detection probe of the detection device are assembled in a vertical arrangement mode, the distance between the incubator and the detection probe of the detection device is short, and then detection operation can be directly carried out after microorganism culture is finished; the condition of pollution in the sample transfer process can be reduced, and the detection quality is improved.
Owner:CHONGQING IND POLYTECHNIC COLLEGE +2
Single-cell crispr-screening of multiple gene perturbations in vivo
The present invention relates to a method allowing for a single-cell-based analysis of multiple CRISPR-mediated gene perturbations in a single organism. The method comprises the administration of a plurality of viral expression vectors each encoding a gRNA into an organism expressing a Cas enzyme, and allows for analysis of the resulting phenotype on a single-cell level.
Owner:ETH ZURICH
A method for pico-coulomb level single bacterium surface charge imaging and application
This invention belongs to the field of microbial electrochemistry and single-cell analysis technology, and specifically relates to a femtocoulomb-level single-bacterial surface charge imaging method and its application. This technology is based on electrochemiluminescence microscopy, utilizing the spontaneous enrichment of cationic ECL luminescent molecules by the electrostatic field of the negative charge on the bacterial surface, thereby non-destructively enhancing its ECL signal. By establishing a quantitative relationship between the ECL intensity enhancement ratio and the number of adsorbed molecules, and combining this with COMSOL simulation to determine the thickness of the luminescent layer, it achieves for the first time high-throughput, high spatiotemporal resolution dynamic quantitative measurement of femtocoulomb-level charges on the surface of a single living bacterium. The invented technology has high sensitivity, high resolution, and good biocompatibility, and can be used to reveal bacterial metabolic-charge regulation mechanisms, analyze synergistic electron transport pathways, and screen highly electroactive bacterial subpopulations in situ, providing a key tool for microbial electrochemical research.
Owner:NANJING UNIV
Use of dextramer in single cell analysis
Disclosed herein include systems, methods, compositions, and kits suitable for the use of dextramers in single cell analysis. In some embodiments, one or more primers allowing generation of separate libraries for proteins (e.g., antibodies) and for dextramers are used.
Owner:AUGUSTA SPINCO CORP +1
Liquid biopsy for diagnosis of early osteoarthritis
PendingUS20250316378A1Mechanical/radiation/invasive therapiesDrug and medicationsDiseaseBlood specimen
Compositions and methods are provided for determining the presence of early-stage osteoarthritis (OA) in an individual by single cell profiling of a blood sample. Through use of machine learning, it is shown that immune cell features associated with OA are present and detectable in the early stages of OA and can be utilized for early detection of the disease.
Owner:THE BOARD OF TRUSTEES OF THE LELAND STANFORD JUNIOR UNIV
Single cell analysis
Provided herein are compositions and methods for accurate and scalable primary template directed amplification (PTA) nucleic acid amplification and sequencing methods, and their applications for mutation analysis in research, diagnosis, and therapy. Further provided herein are multi-omics methods for parallel analysis of DNA, RNA, and / or protein from single cells. Provided herein are multi-omics single-cell analysis methods, comprising: (a) isolating a single cell from a cell population; (b) sequencing a cDNA library comprising polynucleotides amplified from mRNA transcripts from the single cell; (c) sequencing the genome of the single cell.
Owner:BIOSKRYB GENOMICS INC
A single-cell online extension lysis mass spectrometry flow analysis method
ActiveCN116429667BMaterial analysis by electric/magnetic meansAgainst vector-borne diseasesMetaboliteMass spectrometry detector
The application discloses a single-cell online prolonging lysis mass spectrometry flow analysis method. A cell suspension sample is injected into a separation capillary, a front section of the capillary is spirally wound to realize single dispersion of the cells, and a part of a rear section of the capillary is located in an ultrasonic device to realize online ultrasonic lysis, localized single-cell lysis contents are obtained, the content solution gradually enters a non-contact electrospray needle to realize ionization, and finally enters a mass spectrometry detector for detection. Meanwhile, the analysis coverage of weakly polar compounds can be improved by combining a plasma generation device. The method can realize single-cell analysis with high throughput and high metabolite coverage, and by means of a prolonged single-cell detection window, the collection of metabolite primary mass-to-charge ratio information and the identification of secondary structures under high resolution can be realized, including qualitative and quantitative analysis of positional isomers, so that the method has wide application prospects in the fields of cell identification, cell typing, differential substance research, tumor diagnosis and biomarker mining.
Owner:PEKING UNIV
Systems, articles, and methods for flowing particles
Systems and methods for flowing particles, such as biological entities, in a fluidic channel(s) are generally provided. In some cases, the systems described herein are designed such that a single particle may be isolated from a plurality of particles and flowed into a fluidic channel (e.g., a microfluidic channel) and / or collected e.g., on fluidically isolated surfaces. For example, the single particle may be present in a plurality of particles of relatively high density and the single particle is flowed into a fluidic channel, such that it is separated from the plurality of particles. The particles may be spaced within a fluidic channel so that individual particles may be measured / observed over time. In certain embodiments, the particle may be a biological entity. Such article and methods may be useful, for example, for isolating single cells into individual wells of multi-well cell culture dishes (e.g., for single-cell analysis).
Owner:MASSACHUSETTS INST OF TECH
RNA detection and amplification method for single cell analysis on fixed cells
The disclosure includes systems, methods, compositions, and kits for determining the spatial position and / or copy number of a nucleic acid target in a fixed sample. In some embodiments, detection oligonucleotides comprising a coupling sequence, a probe sequence configured to hybridize to a nucleic acid target, and / or a predetermined spatial label are provided. Methods can include contacting each of two or more spatial positions of a sample comprising a copy of a nucleic acid target with the probe oligonucleotide. Methods can include in situ extending a probe oligonucleotide hybridized to a copy of a nucleic acid target to produce more than one extended probe oligonucleotide. In some embodiments, a method may include barcoding more than one extended probe oligonucleotide using more than one oligonucleotide barcode to generate more than one barcoded probe oligonucleotide.
Owner:BECTON DICKINSON & CO
Single cell mapping and transcriptome analysis
Methods of tagging cells with unique oligonucleotide “zipcode” constructs are provided. By these methods and associated compositions, cells in a multicellular structure such as a tissue section can be tagged with a construct, the unique composition of which is associated with the cells position in the multicellular structure. Subsequently, the multicellular structure can be dissociated into single cells and a single cell transcriptome analysis performed, as well as other types of single cell analyses. By preserving positional information in the analyzed single cells, biological processes within the tissue can be mapped. By these methods, the effects of the local environment surrounding a cell on its state and various functions can be elucidated, and intra-tissue processes can be mapped and observed. Likewise, coordinated actions by multiple cells within a tissue can be mapped and tracked over time.
Owner:RGT UNIV OF CALIFORNIA
Liver cancer biomarker and application thereof
PendingCN119391857AImprove reliabilityImprove universalityHealth-index calculationMicrobiological testing/measurementData setBiologic marker
The invention discloses a liver cancer biomarker and application thereof. The liver cancer biomarker comprises MT1G, FOS, TXNRD1 and MYL6. The invention provides a group of liver cancer biomarkers, and a liver cancer risk assessment model is constructed based on the liver cancer biomarkers. The liver cancer biomarker is obtained by synthesizing gene expression level, protein level, single cell analysis and space transcriptome analysis, and the diversity and large sample size of a data set improve the reliability and universality of the liver cancer biomarker and the liver cancer risk assessment model; the sensitivity of the liver cancer risk assessment model in an independent verification data set exceeds 97%, the specificity exceeds 98%, and the liver cancer risk assessment model shows excellent classification performance.
Owner:SHENZHEN PEOPLES HOSPITAL
A transposase-based 5mCpG high-throughput detection method TnM-seq, transposase TnM and single-cell multi-omics application
The application provides a 5mCpG high-throughput detection method TnM-seq based on TnM transposase, which adopts transposase TnM including an MBD domain to detect 5mCpG in situ, and comprises the following steps: after pretreatment of cells to be detected, the cells are incubated with transposase TnM; DNA in the target sample after transposition is recovered and purified; the purified DNA is subjected to PCR library construction; and the library construction product is subjected to high-throughput sequencing. The new transposase TnM newly designed and synthesized is used, combined with high-throughput sequencing, to realize convenient, fast, low-cost and high-sensitivity detection of 5mCpG in a sample. Based on the transposase TnM, a multi-omics technology HAM-seq for simultaneously detecting histone modification H3K9me3, chromatin accessibility and DNA methylation modification 5mCpG in the same sample is further developed, and after being combined with a commercial single-cell microfluidic platform, an epigenome and gene expression four-omics technology EpiX-seq for single-cell analysis in a complex tissue is obtained.
Owner:ZHEJIANG UNIV
Single-cell profiling of phagocytosis using microscopy
The invention relates to methods of evaluating phagocytosis at a single-cell level by placing one or more phagocytes and one or more particles on a surface and visualizing the phagocytosis of the particles by the phagocytes. The invention also relates to systems for evaluating phagocytosis of a particle by a phagocyte at a single-cell level.
Owner:UNIV HOUSTON SYST
Method for automatically detecting abnormal cells in urine exfoliated cells
PendingCN120629142APreparing sample for investigationMaterial analysis by optical meansTest sampleAbnormal cells
The invention provides a method for automatically detecting abnormal cells in urine cast-off cells, which comprises the following steps of: training an abnormal cell frame selection recognition algorithm, and generating an abnormal cell frame selection recognition model; according to the abnormal cell frame selection recognition model, abnormal cell frame selection recognition of a to-be-tested sample is achieved. The method is suitable for early preliminary diagnosis screening and postoperative reexamination of bladder urothelium carcinoma, has the advantages of early noninvasive detection, convenience in analysis and report, simplicity and standardization in operation, suitability for automatic analysis of instruments and the like, and not only can eliminate non-cell impurities in a complex background, but also can effectively separate overlapped cells, so that the detection accuracy is improved. And a foundation is laid for subsequent single cell analysis.
Owner:FIRST AFFILIATED HOSPITAL OF DALIAN MEDICAL UNIV
Integrated device for comprehensive tissue to single-cell sample preparation
PendingUS20250052651A1Preparing sample for investigationLaboratory glasswaresTissue sampleBiomedical engineering
Described herein are methods and devices directed to preparing complex tissue samples for direct single cell analysis (e.g., scRNAseq) in a comprehensive, enzyme-free manner. The device and method use shear forces, mechanical features, electrical treatment, and acoustics, in addition to programmed microfluidic flow to dissociate a tissue sample into a purified suspension of single cells. In an example, a tissue sample is loaded into a well with retaining mesh on either side. A combination of microfluidic flow, shear, and electrical treatment elutes clumps (e.g., aggregates or agglomerates) of cells from this well, while a sample-specific, back and forth flow is repeated to begin an impurity removal process. At an outlet of the device, purified cells suitable for single cell analysis are eluted. As described herein, the device can be operated continuously.
Owner:BROWN UNIVERSITY
Microfluidic chip (for use in single cell sorting based on label-free or fluorescence detection)
1. Name of the Design Product: Microfluidic Chip (for Single-Cell Sorting Based on Label-Free or Fluorescent Detection). 2. Use of the Design Product: Microfluidic chip, for biological particles such as cells, implementing applications such as single-cell analysis, manipulation, and sorting based on label-free or labeled detection methods. 3. Design Key Points of the Design Product: Lies in the shape. 4. Picture or Photograph Most Illustrating the Design Key Points: Perspective view. 5. Since it is already shown in other views, the right view and the bottom view are omitted. 6. Part A is transparent or semi-transparent.
Owner:DALIAN HUAWEI LIFE TECH CO LTD
A ratiometric bipolar electrode electrochemiluminescence biosensor based on quasi-homogeneous reaction and its application
ActiveCN116818865BMaterial electrochemical variablesAgainst vector-borne diseasesMagnetite NanoparticlesElectrochemiluminescence
The present invention discloses a ratiometric bipolar electrode electrochemiluminescence biosensor based on quasi-homogeneous reaction and its application. 2+ Using the DNA / TPrA system as a dual-anode signal source, a novel ratiometric bipolar electrode electrochemiluminescence biosensor for miRNA detection and analysis was constructed. By combining the ligase chain reaction with CRISPR / Cas technology and integrating gold nanoparticle-modified Fe3O4 magnetic nanoparticles enriched with ferrocene nucleic acid signal probes (DNA-Fc / Au@Fe3O4 NPs) as a multifunctional reaction interface, the sensor effectively modulates the signal changes of the two ECL reaction systems. Ultrasensitive detection of miRNA-222 is achieved through the ratio of the two signals. The constructed sensor exhibits exceptional performance for miRNA-222 detection and is expected to be applied as a novel detection method in early clinical diagnosis and single-cell analysis.
Owner:FUJIAN MEDICAL UNIV