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17 results about "Single-cell analysis" patented technology
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In the field of cellular biology, single-cell analysis is the study of genomics, transcriptomics, proteomics and metabolomics at the single cell level. Due to the heterogeneity seen in both eukaryotic and prokaryotic cell populations, analyzing a single cell makes it possible to discover mechanisms not seen when studying a bulk population of cells. Technologies such as fluorescence-activated cell sorting (FACS) or digital dielectrophoretic sorting (DEPArray), allow the precise isolation of selected single cells from complex samples, while high throughput single cell partitioning technologies, enable the simultaneous molecular analysis of hundreds or thousands single unsorted cells; this is particularly useful for the analysis of transcriptome variation in genotypically identical cells, allowing the definition of otherwise undetectable cell subtypes. The development of new technologies is increasing our ability to analyze the genome, and transcriptome, of single cells, as well as to quantify their proteome and metabolome. New developments in mass spectrometry techniques have become important analytical tools for proteomic and metabolomic analysis of single cells. In situ sequencing and fluorescence in situ hybridization (FISH) do not require that cells be isolated and are increasingly being used for analysis of tissues.
A method for pico-coulomb level single bacterium surface charge imaging and application
This invention belongs to the field of microbial electrochemistry and single-cell analysis technology, and specifically relates to a femtocoulomb-level single-bacterial surface charge imaging method and its application. This technology is based on electrochemiluminescence microscopy, utilizing the spontaneous enrichment of cationic ECL luminescent molecules by the electrostatic field of the negative charge on the bacterial surface, thereby non-destructively enhancing its ECL signal. By establishing a quantitative relationship between the ECL intensity enhancement ratio and the number of adsorbed molecules, and combining this with COMSOL simulation to determine the thickness of the luminescent layer, it achieves for the first time high-throughput, high spatiotemporal resolution dynamic quantitative measurement of femtocoulomb-level charges on the surface of a single living bacterium. The invented technology has high sensitivity, high resolution, and good biocompatibility, and can be used to reveal bacterial metabolic-charge regulation mechanisms, analyze synergistic electron transport pathways, and screen highly electroactive bacterial subpopulations in situ, providing a key tool for microbial electrochemical research.
Owner:NANJING UNIV
Single-cell profiling of phagocytosis using microscopy
The invention relates to methods of evaluating phagocytosis at a single-cell level by placing one or more phagocytes and one or more particles on a surface and visualizing the phagocytosis of the particles by the phagocytes. The invention also relates to systems for evaluating phagocytosis of a particle by a phagocyte at a single-cell level.
Owner:UNIV HOUSTON SYST
A liver cancer prognosis marker and application thereof
The application discloses a liver cancer prognosis marker and application thereof, and the liver cancer prognosis marker comprises TXNRD1, HAO1, C1S, OPTN and SNX6. The application provides a group of liver cancer prognosis markers, and a liver cancer patient prognosis prediction model is constructed based on the liver cancer prognosis markers. The liver cancer prognosis markers are obtained through comprehensive gene expression levels, single cell analysis and spatial transcriptome analysis, and the diversity of the data set and the large sample quantity improve the reliability and universality of the liver cancer prognosis markers and the liver cancer patient prognosis prediction model.
Owner:SHENZHEN PEOPLES HOSPITAL
Use of ndufb4 as a target in the preparation of a drug for treating bladder cancer
The application discloses application of NDUFB4 as a target point in preparation of a drug for treating bladder cancer. Single cell analysis results show that NDUFB4 is highly enriched in bladder cancer epithelial cells, and can be used as a key coordinator to participate in the regulation of multiple carcinogenic signal pathways. Through shRNA-mediated gene silencing and CRISPR / Cas9-mediated gene knockout experiments, it is found that NDUFB4 deletion can significantly inhibit the proliferation, migration and invasion ability of bladder cancer cells, and cause a serious bioenergy crisis, and activate the endogenous apoptosis pathway. In a subcutaneous xenotransplant tumor model, it is further verified that NDUFB4 silencing can significantly inhibit tumor growth. The application discloses that NDUFB4 is a key coordinator between mitochondrial high-functionality and carcinogenic signal pathways, and it is suggested that NDUFB4 can become a potential target for bladder cancer treatment.
Owner:CHANGZHOU NO 2 PEOPLES HOSPITAL
Systems, compositions, and methods for single cell analysis
Provided herein are systems, compositions, and methods for single cell analysis. In particular, provided herein are systems, compositions, and methods for scalable, high-throughput isolation and sequencing of nucleic acids from rare and / or fragile single cells.
Owner:FLUID DISCOVERY
Single-cell analysis system and method based on precise typing of lung cancer immune microenvironment and treatment prediction
PendingCN122290983AClinical efficacySingle cell transcriptome
This invention relates to the fields of bioinformatics and precision oncology medicine, specifically disclosing a single-cell analysis system and method based on precise typing and treatment prediction of the lung cancer immune microenvironment. The system includes a data preprocessing and quality control module, a lung cancer-specific cell annotation module, an immune microenvironment typing module, a clinical efficacy prediction module, and a visualization report generation module. These modules form a complete technical chain from raw data to clinical efficacy prediction. Furthermore, this invention provides a scheme for precise typing and treatment prediction of the lung cancer immune microenvironment matching this system. This invention can take single-cell transcriptome data (which can integrate spatial transcriptome data) from lung cancer patients as input, process it through a series of specific computational modules, and finally output immune microenvironment typing results and treatment response prediction results with clear clinical guidance significance, solving the problems of unclear typing, fragmented processes, and insufficient clinical translation capabilities in existing technologies.
Owner:WEST CHINA HOSPITAL SICHUAN UNIV
A three-dimensional flow cytometer and method based on optical detection and ICP-MS
The application designs a three-dimensional flow cytometer and method based on optical detection and ICP-MS combination, relates to the technical field of life analysis scientific detection equipment; first, a three-dimensional flow cytometer is provided, a three-dimensional flow cytometer method based on optical detection and ICP-MS combination is designed based on the analyzer, and the flow cytometer can only perform single-parameter flow cytometer analysis of fluorescence, scattering and mass spectrum or multi-parameter flow cytometer analysis in any combination; the data processing software in the analyzer can provide offline processing of flow cytometer analysis data of fluorescence, side scattering light and mass spectrum, identify, calculate and statistically analyze single-cell pulse signals, especially can provide integration and unification among fluorescence, side scattering and mass spectrum single-cell signals, and obtain three-parameter information of the same cell.
Owner:NORTHEASTERN UNIV CHINA
A method for detecting circulating tumor cells based on the expression amount of sialic acid on the cell membrane surface and single cell detection technology
The application discloses a method for detecting circulating tumor cells based on cell membrane surface sialic acid expression quantity and single cell detection technology, and belongs to the field of biomedical technology. The method comprises the following steps: using a boron acid group modified probe to label cell surface sialic acid in a blood sample after erythrocyte lysis; passing the labeled sample through a phase transfer flow focusing chip to enrich and purify circulating tumor cells based on cell size; and detecting the enriched and purified cells by a single cell analysis method. The application solves the problems of high cost, long time consumption and high missed detection rate of the current circulating tumor cell detection method applied in clinical practice, and provides a method with simple operation and higher universality for circulating tumor cell detection.
Owner:WUHAN UNIV
Mass tags for mass cytometry and methods of making and using the same
This invention provides a mass tag for mass flow cytometry, its preparation method, and its application. The mass tag provided by this invention has the chemical structure shown in Formula 1. The compound shown in Formula 1 provided by this invention offers a new channel for CyTOF single-cell analysis, suitable for the detection of various biomarkers such as live cells and proteins. It is of great significance for more in-depth and comprehensive analysis of changes in various cell subtypes and their proportions, and for predicting the changing trends of clinical disease courses, showing good clinical application prospects and significant social benefits.
Owner:PEKING UNIV
Single cell 3'end transcriptome variable poly-adenosine acidification analysis method
PendingCN122081469Aaccurate captureImprove detection accuracyMicrobiological testing/measurementSequence analysisPolyadenylationTranscriptome Sequencing
The invention discloses a single cell 3'end transcriptome variable polyadenylation (APA) analysis method, and belongs to the field of bioinformatics. The analysis process provided by the invention is based on 3'end transcriptome sequencing data of a single cell, combines multiple processes of UMI deduplication, false comparison removal, site merging, internal amplification removal, polyA site removal with few supporting cells, site verification and the like, and can realize efficient detection and quantification of APA at the single cell level. The method is especially suitable for single cell APA analysis of single cell 3'end transcriptome sequencing data, including 3 'end single cell sequencing data after 10x scRNA-Seq cell subset combination, 3' end single cell sequencing data based on a C1 chip, and even full-length transcriptome data.
Owner:HENAN CANCER HOSPITAL
Method for rapid and large-scale generation of droplets and droplet libraries
Provided is a method of generating droplets that includes aspirating a first liquid into a tube, positioning the tube over a receiving liquid, and ejecting the first liquid to generate a plurality of droplets that contact the receiving liquid and remain discrete even after contacting the receiving liquid. Whereas many other droplet generators require complex microfluidics, the present methods allow generation of droplets without the need for microfluidics. The methods can be performed with existing commercially available macro-fluidic liquid handling devices. The methods can be used for digital PCR, digital MDA, enzyme screening, single cell analysis, and other applications involving droplets.
Owner:RGT UNIV OF CALIFORNIA
Single-cell analysis container and single-cell analysis method using the same
To provide a container for single cell analysis that enables a solid phase in a single cell analysis device to be readily and rapidly retrieved into a separate container, can reduce the time for nucleic acid treatment reactions, and enables applying a wide range of nucleic acid treatment reactions, and to provide a single cell analysis method using the same.SOLUTION: A container for single cell analysis 21 comprises: a reaction substrate comprising a cell capture part 7 that captures cells 31 and one or more micro reaction tanks 8 that are disposed directly under the cell capture part 7 and filled with a solid phase; and a cell holding part 7 that has the reaction substrate as its bottom and is configured to hold a solution containing the cells 31.SELECTED DRAWING: Figure 2
Owner:HITACHI LTD
Carbon-based field effect transistor combined with microfluidics for single-cell analysis and application, method for detecting surface membrane protein of single cell
ActiveCN117643928BBioreactor/fermenter combinationsBiological substance pretreatmentsCell trappingCell Surface Proteins
The application provides a single cell analysis chip combining a carbon-based field effect transistor and microfluidics, comprising a plurality of single cell capture units; wherein the single cell capture unit comprises a sensing unit and a PDMS microfluidic channel unit arranged correspondingly to the sensing unit; the sensing unit is a carbon-based field effect transistor, and the PDMS microfluidic channel unit comprises a circular capture trap chamber vertically and directly above a sensing area of the carbon-based field effect transistor and a narrow channel; a first end of the circular capture trap chamber is connected with a first end of a first main channel, a second end of the circular capture trap chamber is connected with a first end of a second main channel through the narrow channel, and the first main channel and the second main channel are connected through an arc-shaped side channel; after cells are captured through the narrow channel, subsequent cells can flow into the next PDMS microfluidic channel unit through the arc-shaped side channel. The chip can separate and capture single cells, and achieve the purpose of detecting the surface proteins of single cells.
Owner:XIANGTAN UNIV
Container for single cell analysis and single cell analysis method using same
PendingEP4541879A4Bioreactor/fermenter combinationsBiological substance pretreatmentsTesting MethodsCell biology
The present invention relates to a container for single cell analysis and a single cell analysis method using the same. The present invention also relates to an automated device for performing single cell analysis using the container for single cell analysis. Specifically, the present invention provides a container for single cell analysis which includes: a reaction substrate including a cell trapping portion on which a cell is trapped, and one or a plurality of microreaction chambers arranged immediately below the cell trapping portion and filled with a solid phase; and a cell holding portion having the reaction substrate as a bottom and configured to hold a solution containing cells.
Owner:HITACHI LTD
Single-cell transcriptomics analysis and multimodal profiling
PCT designated stageWO2026112166A1Microbiological testing/measurementLaboratory glasswaresSomatic cellSingle cell transcriptomics
A sample preparation, computational system, sample analysis workflow, and associated methods are presented herein which utilize imaging-based transcriptomics and proteomics in place of conventional single cell sequencing methods to significantly scale-up cell numbers at substantially reduced costs for single cell analysis, while preserving the advantages of molecule detection at a single-cell level. Traditionally, spatial transcriptomics and proteomics have been applied in the context of tissue profiling to map the composition and architecture of complex samples, such as organs or tumors. A sample preparation including one or more monolayers of individual cells is analyzed using image-based transcriptomics and proteomics equipment which can include software to take advantage of new aspects of the sample preparation.
Owner:ST JUDE CHILDRENS RES HOSPITAL INC +1
Systems and methods for an artificial intelligence based workflow assistant for single cell analysis
Various embodiments for systems and methods of providing an artificial intelligence (AI) workflow assistant for single cell RNA sequencing (scRNA-seq) and related "omics" analyses within an R / Seurat environment is disclosed herein. Input data including a Seurat object and associated outputs are processed to compute dataset metrics that are combined with pre-defined prompt templates to internally construct augmented prompts, without requiring users to manually craft prompts. Augmented prompts are submitted to a large language model (LLM), and responses are parsed and integrated into workflow outputs to provide data-driven recommendations and interpretations across analysis stages. In some implementations, an integration and analysis network (IAN) uses a multi-agent architecture in which multiple agents process pathway and network-based enrichment results, aggregate agent responses with an experimental description to form a comprehensive augmented prompt, and generate an integrated LLM response, an LLM-derived system model, and a comprehensive HTML report, optionally including interactive network visualizations summarizing enriched pathways and relationships.
Owner:THE GOVERNMENT OF THE UNITED STATES OF AMERICA AS REPRESENTED BY THE SECRETARY DEPARTMENT OF HEALTH & HUMAN SERVICES
