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138 results about "Single-cell analysis" patented technology
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In the field of cellular biology, single-cell analysis is the study of genomics, transcriptomics, proteomics and metabolomics at the single cell level. Due to the heterogeneity seen in both eukaryotic and prokaryotic cell populations, analyzing a single cell makes it possible to discover mechanisms not seen when studying a bulk population of cells. Technologies such as fluorescence-activated cell sorting (FACS) or digital dielectrophoretic sorting (DEPArray), allow the precise isolation of selected single cells from complex samples, while high throughput single cell partitioning technologies, enable the simultaneous molecular analysis of hundreds or thousands single unsorted cells; this is particularly useful for the analysis of transcriptome variation in genotypically identical cells, allowing the definition of otherwise undetectable cell subtypes. The development of new technologies is increasing our ability to analyze the genome, and transcriptome, of single cells, as well as to quantify their proteome and metabolome. New developments in mass spectrometry techniques have become important analytical tools for proteomic and metabolomic analysis of single cells. In situ sequencing and fluorescence in situ hybridization (FISH) do not require that cells be isolated and are increasingly being used for analysis of tissues.
Single cell gene expression for diagnosis, prognosis and identification of drug targets
InactiveUS20100255471A1Microbiological testing/measurementDisease diagnosisDrug targetDisease status
Methods are provided for diagnosis and prognosis of disease by analyzing expression of a set of genes obtained from single cell analysis. Classification allows optimization of treatment, and determination of whether on whether to proceed with a specific therapy, and how to optimize dose, choice of treatment, and the like. Single cell analysis also provides for the identification and development of therapies which target mutations and / or pathways in disease-state cells.
Owner:THE BOARD OF TRUSTEES OF THE LELAND STANFORD JUNIOR UNIV
Single cell analysis by polymerase cycling assembly
ActiveUS20110207617A1Microbiological testing/measurementLibrary screeningNucleic acid sequencingPolymerase cycling assembly
The invention provides a method of making measurements on individual cells of a population, particularly cells that have identifying nucleic acid sequences, such as lymphoid cells. In one aspect, the invention provides a method of making multiparameter measurements on individual cells of such a population by carrying out a polymerase cycling assembly (PCA) reaction to link their identifying nucleic acid sequences to other cellular nucleic acids of interest. The fusion products of such PCA reaction are then sequenced and tabulated to generate multiparameter data for cells of the population.
Owner:ADAPTIVE BIOTECH
Single cell analysis using sequence tags
InactiveUS20150247182A1Microbiological testing/measurementLibrary member identificationNucleic acid sequencingPolymerase cycling assembly
The invention provides a method of making measurements on individual cells of a population by forming reactors containing single cells and a predetermined number, usually one, homogeneous sequence tag. In one aspect, the invention provides a method of making multiparameter measurements on individual cells of such a population by carrying out a polymerase cycling assembly (PCA) reaction to link their identifying nucleic acid sequences, such as sequence tag copies derived from a homogeneous sequence tag, to other cellular nucleic acids of interest, thereby forming fusion products. The fusion products of such PCA reactions are then sequenced and tabulated to generate multiparameter data for cells of the population.
Owner:ADAPTIVE BIOTECH
Micro-fluidic chip and micro-fluidic chip system for single cell analysis and single cell analyzing method
ActiveCN103894248AAchieve crackingHigh precisionLaboratory glasswaresFluorescence/phosphorescenceCell trappingEngineering
The invention relates to a micro-fluidic chip and a micro-fluidic chip system for single cell analysis and a single cell analyzing method. The micro-fluidic chip for single cell analysis comprises a transparent top cover, a microfluid channel, a substrate with a micropore array and a bottom plate with a cavity body, which are sequentially connected from top to bottom in a sealing manner, wherein the transparent top cover is provided with an inflow hole and an outflow hole which are used for communicating the outside and the microfluid channel, the micropore array of the substrate is arranged below the microfluid channel and is communicated with the microfluid channel, each micropore is internally provided with an electrode, each electrode is provided with a cathode and an anode, each micropore can contain a single cell only, the bottom of each micropore is provided with a channel which is communicated with the cavity body of the bottom plate, and the cross section of the channel is smaller than the size of each single cell. With the adoption of the micro-fluidic chip, the micro-fluidic chip system and the single cell analyzing method, the capturing, identifying and disruption processes of each single cell are integrated on one micro-fluidic chip; as a corresponding operation method is provided, the content of each single cell is rapidly acquired and accurately analyzed.
Owner:THE NAT CENT FOR NANOSCI & TECH NCNST OF CHINA
High-throughput single-cell analysis combining proteomic and genomic information
ActiveUS20160244828A1Predict susceptibilityMaterial analysis using sonic/ultrasonic/infrasonic wavesMicrobiological testing/measurementProteomicsPolynucleotide
Disclosed herein are methods for single-cell sequencing. In some examples, the methods include enriching a sample comprising a plurality of cells for cells of interest to produce an enriched cell sample; isolating one or more cells of interest in the enriched cell sample; and obtaining sequence information of one or more polynucleotides from each of the one or more isolated cells. Obtaining sequence information may include generating a molecularly indexed polynucleotide library from the one or more isolated cells. Enriching the sample may include focusing cells of interest in the sample using acoustic focusing.
Owner:BECTON DICKINSON & CO
Cell enriching, separating and extracting method and instrument and single cell analysis method
ActiveCN102690786AEnrichment reachedEfficient collectionMicrobiological testing/measurementBiomass after-treatmentImmunofluorescence stainingMagnetic bead
The invention discloses a method and an automatic instrument device for enriching and extracting target cells and separating single cells by using a positive magnetic bead method and performing immunity and molecular biology identification and analysis on single cells. By the method and the instrument device, the on / off of a capture magnet and a release magnet is controlled by various methods to complete target cell searching, capturing, cleaning and releasing operation once or for multiple times, so that the target cell detection sensitivity and stability are improved. When the capture magnet searches and captures the target cells, the search line is circular, square, comb-shaped, S-shaped or U-shaped. In addition, the captured substances are filtered, most free micro magnetic beads are removed, the purity of the product is further improved and the product can be used as a good experimental material. By combining a special filter and the adsorption of the capture magnet, more than 95 percent of free micro magnetic beads can be effectively removed. Meanwhile, the types of the single cells are identified and biological characteristics of the single cells are analyzed by an immunofluorescence staining method and a method in molecular biology, and effective biological indexes are provided for clinical diagnosis and treatment of cancers.
Owner:GD TECH INC
Methods and Apparatus for Magnetic Separation of Cells
ActiveUS20090220979A1Increase the magnetic field strengthStir wellBioreactor/fermenter combinationsBiological substance pretreatmentsSuperparamagnetic beadsTumor cells
Described here is an automated robotic device that isolates circulating tumor cells (CTCs) or other biological structures with extremely high purity. The device uses powerful magnetic rods covered in removable plastic sleeves. These rods sweep through blood samples, capturing, e.g., cancer cells labeled with antibodies linked to magnetically responsive particles such as superparamagnetic beads. Upon completion of the capturing protocol, the magnetic rods undergo several rounds of washing, thereby removing all contaminating blood cells. The captured target cells are released into a final capture solution by removing the magnetic rods from the sleeves. Additionally, cells captured by this device show no reduced viability when cultured after capture. Cells are captured in a state suitable for genetic analysis. Also disclosed are methods for single cell analysis. Being robotic allows the device to be operated with high throughput.
Owner:THE BOARD OF TRUSTEES OF THE LELAND STANFORD JUNIOR UNIV
Methods and systems for analysis of single cells
Owner:THE BOARD OF TRUSTEES OF THE LELAND STANFORD JUNIOR UNIV
Single cell gene expression for diagnosis, prognosis and identification of drug targets
Methods are provided for diagnosis and prognosis of disease by analyzing expression of a set of genes obtained from single cell analysis. Classification allows optimization of treatment, and determination of whether on whether to proceed with a specific therapy, and how to optimize dose, choice of treatment, and the like. Single cell analysis also provides for the identification and development of therapies which target mutations and / or pathways in disease-state cells.
Owner:THE BOARD OF TRUSTEES OF THE LELAND STANFORD JUNIOR UNIV
Methods and apparatus for magnetic separation of cells
ActiveUS8071395B2Removing the magnetic fieldIncrease the magnetic field strengthBioreactor/fermenter combinationsBiological substance pretreatmentsSuperparamagnetic beadsCancer cell
Described here is an automated robotic device that isolates circulating tumor cells (CTCs) or other biological structures with extremely high purity. The device uses powerful magnetic rods covered in removable plastic sleeves. These rods sweep through blood samples, capturing, e.g., cancer cells labeled with antibodies linked to magnetically responsive particles such as superparamagnetic beads. Upon completion of the capturing protocol, the magnetic rods undergo several rounds of washing, thereby removing all contaminating blood cells. The captured target cells are released into a final capture solution by removing the magnetic rods from the sleeves. Additionally, cells captured by this device show no reduced viability when cultured after capture. Cells are captured in a state suitable for genetic analysis. Also disclosed are methods for single cell analysis. Being robotic allows the device to be operated with high throughput.
Owner:THE BOARD OF TRUSTEES OF THE LELAND STANFORD JUNIOR UNIV
Single cell analyses
ActiveUS20190218607A1Improve robustnessRaise the ratioMicrobiological testing/measurementPhotomechanical apparatusGeneticsSingle-cell analysis
This disclosure describes improvements to both hardware and enzymatic reactions used in single cell analyses such as but not limited to Seq-well that enable significant increases in the yield of transcripts per cell, improved portability and ease of use, increased scalability of the assay, and linkage of transcript information to other measurements made in the picowell arrays.
Owner:MASSACHUSETTS INST OF TECH
Full-automatic single particle/single cell capturing chip based on digital microfluids technique and application thereof
ActiveCN107904163AReduce consumptionFully automaticBioreactor/fermenter combinationsBiological substance pretreatmentsCouplingControl system
The invention discloses a full-automatic single particle / single cell capturing chip based on a digital microfluids technique and application thereof. The full-automatic single particle / single cell capturing chip comprises a digital microfluids chip with multiple pattern layers and an integrated circuit, wherein the digital microfluids chip with multiple pattern layers consists of an upper electrode plate and a lower electrode plate; the upper electrode plate adopts lyophobic conductive glass as a ground electrode; the integrated circuit is used as a digital microfluids circuit control system.The full-automatic single particle / single cell capturing chip has the advantages that the single particles / single cells can be fully automatically captured, the operation is simple and quick, and thecapturing efficiency is high; a sample can be recycled without damage, and the capturing chip is especially suitable for rare samples; the continuous and complicated parallel operation of the capturing unit is realized by program control, and the crossing pollution is avoided; the capturing chip can be widely applied to the fields of detection of single particles and analysis of single cells, suchas isolation, coupling and detection of single particles, and isolation, culture and nucleic acid amplification of single cells.
Owner:WISDOM HEALTHY BIOTECH XIAMEN CO LTD
Two-laser induced fluorescence multi-color detector used for single-cell electrophoretic chip
InactiveCN105738331AHigh sensitivityGood repeatabilityFluorescence/phosphorescenceMicroscopic observationElectrophoresis
The invention discloses a two-laser induced fluorescence multi-color detector used for a single-cell electrophoretic chip.The detector is composed of a laser excitation module, a fluorescence collection and detection module, a microimaging module, a signal collection and processing module and a microfluidic chip detection platform and has the functions of performing two-laser induced fluorescence multi-color detection, normal microscopic observation, two-laser spot focusing and detection area alignment observation and the like on a microfluidic chip.By means of the detector, simultaneous detection and quantification of fluorescence substances with different light absorption and fluorescence characteristics, especially fluorescence labeling multi-component activated small molecules, with different excitation and different emission, in single cells of electrophoretic separation of the microfluidic chip can be achieved, and space of relative research and application of laser induced fluorescence detection, the microfluidic chip, single-cell analysis, detection of activated small molecules and the like is greatly expanded.
Owner:SHANDONG NORMAL UNIV
Apparatus for forming and screening two-dimensional liquid droplet array, and use method thereof
The present invention discloses an apparatus for forming and screening a large-scale single-layer two-dimensional liquid droplet array, and a use method thereof. The apparatus comprises a micro-column array chip, a scraping plate for auxiliary spreading of a liquid droplet, a probe for controlling the liquid droplet, a three-dimensional translation table and other components, wherein a micro groove unit for trapping the single liquid droplet is formed by using the gap between micro-columns, and the liquid droplet is spread in the micro groove unit in a single-layer and single manner through gravity effect and capillary effect or through an auxiliary means so as to form the single-layer liquid droplet array. With the apparatus of the present invention, the accurate positioning and detection on the liquid droplet, the sucking on the target liquid droplet and the subsequent treatments can be performed. According to the present invention, the apparatus has characteristics of simple structure and easy operation; and the method has advantages of fast liquid droplet spreading, high flux, high liquid droplet trapping efficiency, liquid droplet position fixing and the like, and is suitable for single cell analysis and screening, single molecule analysis and screening, high-throughput gene screening, protein directed evolution, antibody screening, microbial research, drug screening, and other fields.
Owner:ZHEJIANG UNIV
Micro-fluidic chip for capturing single cell/single granule through high-flux pairing and application of micro-fluidic chip
ActiveCN107012067AReduce consumptionImprove throughputBioreactor/fermenter combinationsBiological substance pretreatmentsControl layerHigh flux
The invention relates to a micro-fluidic chip for capturing single cells / single granules through high-flux pairing and application of the micro-fluidic chip. The micro-fluidic chip comprises three parts, namely a capturing layer, a control layer and a carrier from top to bottom, wherein the capturing layer consists of two runners in parallel and a connection channel; the control layer is an isolation pump layer; a plurality of single granule / single cell capturing units are arranged inside the runners and are used for capturing single granules / single cells; the connection channel is used for communicating two paired capturing units; isolation channels are positioned below the connection channel, are perpendicular to the connection channel and are isolated by diaphragms. The micro-fluidic chip is capable of capturing single granules / single cells in a high flux manner, is high in capturing efficiency, capable of achieving pairing of single granules / single granules, single cells / single cells and single granules / single cells, high in pairing efficiency, capable of achieving parallel operation and control on paired units, such as isolation, culture, cracking and nucleic acid amplification of single cells, can be widely applied to the field of single cell analysis, and is applicable to cell samples of small numbers.
Owner:苏州德运康瑞生物科技有限公司
Capillary needle, and electro-spray ionization mass spectrometry analytical apparatus and method
ActiveCN104392886AFast samplingSmall amount of sampleSamples introduction/extractionMaterial analysis by electric/magnetic meansImaging analysisMedicine
The invention discloses a capillary needle. The capillary needle comprises a tube body, the two ends of the tube body are respectively provided with an inlet and an outlet for electro-spray, a part of the tube body is bent to form a sampling end, and the sampling end is provided with a sampling port communicating with a cavity in the tube body. The invention also discloses an electro-spray ionization mass spectrometry analytical apparatus applying the capillary needle. The invention also simultaneously discloses a method applying the electro-spray ionization mass spectrometry analytical apparatus for analytical detection. The analytical apparatus is high in integration, compact in structure, easy to control, small in size and low in cost; the sample consumption is greatly reduced, and the experiment cost is decreased; an integrated sample introduction method is employed, and an sample introduction end does not have to be repeatedly placed in a sample and blank solution, such that cross contamination is reduced, and more importantly, the analytical flux is substantially improved; and through combination with an automatic sample changing system, different samples can be automatically introduced, and therefore, the capillary needle, the apparatus and the method are quite suitable for such fields as high-flux screening, unicell analysis, mass spectrum imaging analysis and the like.
Owner:ZHEJIANG UNIV
Methods for diagnosing solid tumors
InactiveUS20130129681A1Peptide/protein ingredientsMicrobiological testing/measurementTherapeutic treatmentWilms' tumor
An embodiment of the present invention is useful for identifying tumor cells in bladder cancer washes, pleural effusions, biliary tumor cells and circulating tumor cells in whole blood. Subsequent analysis may identify therapeutic treatments based on a single cell analysis of activatable elements in cell signaling pathways. This analysis can be useful for diagnosis, prognosis, therapy selection and monitoring of solid tumor diseases.
Owner:NODALITY
Preparation method of surface-modified fluorescent magnetic polymer composite microspheres
InactiveCN102861541AOrganic/organic-metallic materials magnetismLuminescent compositionsOil phaseFluorescence microscope
The invention provides a preparation method of surface-modified fluorescent magnetic polymer composite microspheres. The method comprises the steps of preparing a dextran-modified nano-scale Fe3O4 dispersion liquid, preparing a mixed surfactant, preparing an oil phase, preparing an emulsion, preparing an aqueous phase, preparing a magnetic nano-scale Fe3O4 copolymer composite micro-gel, preparing SiO2-coated nano-scale Fe3O4 copolymerized magnetic polymer composite microspheres, and preparing the surface-modified fluorescent magnetic polymer composite microspheres. The prepared surface-modified fluorescent magnetic polymer composite microspheres are tested by using a fluorescence microscope, a scanning electron microscope, a fluorescence spectrometer, and a vibrating sample magnetometer; the test results show that the microspheres have the characteristics of high fluorescent luminous emissivity, good magnetic responsibility and stable performance, the microspheres have the rigidity and stability of inorganic materials and the flexibility of organic materials, and the microspheres can be applied to the aspects of immunoassay, drug carriers, immobilized enzyme, single nucleotide polymorphism genotype, cytokine identification, single-cell analysis and the like.
Owner:SHAANXI NORMAL UNIV
Single Cell Analysis of Membrane Molecules
InactiveUS20090173631A1High sensitivitySludge treatmentVolume/mass flow measurementTarget surfaceCell culture media
Interrogation of surface receptors of individual cells within a population of cell types in a single mixture is described. Each cell comprises, bound to target surface receptors on the cell, a collection of binding compositions, each comprising a distinct molecular tag. A continuous flow of cell medium containing the cells is pneumatically transported through a channel in a separation device, toward a cleavage / release zone, where specific tags correlating to each binding composition, and thus to each corresponding cell surface receptor, are released from the binding compositions on the cell surface. The method is effective to produce a separately detectable collection of tag signals for each cell.
Owner:MONOGRAM BIOSCIENCES
Digital control system and digital control method for living single cell Raman analytic platform
ActiveCN105588827AImprove throughputComprehensive support and perfect control partRaman scatteringData acquisitionDigital control
The invention relates to the field of single cell sorting, particularly, a digital control system and a digital control method for a living single cell Raman analytic platform. The system comprises an instrument control module, a cell image recognizing and analyzing module, a Raman analysis treatment module and a single cell screening control module. The digital control method includes the following steps: a) inputting a sample, collecting data, and performing edge detection and coordinate recognition; b) controlling an X axis, a Y axis and a Z axis to move to the position that the cell is located by means of the instrument control module according to the coordinate of the cells; c) comparing a single cell Raman spectrum with a Raman spectrum database to obtain information of the single cells; d) when the single cells satisfying conditions arrives at a selection branch position of a microfluidic chip, controlling an electromagnetic valve to push the single cells into a cell screening outlet by means of the instrument control module, or otherwise, enabling the single cells to flow into a waste liquid tank. The system and the method can form a systematized and complete operation platform for comprehensively supporting a control part of a high-throughput in-situ living single cell optical tweezers screening system, thereby promoting researching on single cell analysis.
Owner:青岛星赛生物科技有限公司
Method and apparatus for detecting bacterial resistance by single cell analysis
ActiveCN109266717AEasy to handleFast measurementBioreactor/fermenter combinationsBiological substance pretreatmentsCollection systemLaser scanning
A method for detect bacterial drug resistance by single cell analysis comprises mixing a certain amount of bacterial sample and a certain amount of antibiotic diluent on a slide, covering that slide with a cover glass and sealing with a sealant on four side to form a plate-type microbiochemical reaction system in which the bacterial and antibiotic diluent react biochemically. Bacteria were observed and located by phase contrast microscope and Raman spectra were obtained by laser scanning. Bacterial drug resistance could be obtained by analysis. The invention also provides a device for realizing the method, and the device comprising a plate-type micro-biochemical reaction system, a phase contrast microscope, a high-power optical microscope, a Raman spectrum excitation system and a Raman spectrum collection system. Bacteria were observed by phase contrast microscope and high power optical microscope, and then Raman spectra were obtained by Raman spectrum excitation system and Raman spectrum collection system to detect bacterial drug resistance.
Owner:上海镭立激光科技有限公司
Single cell analysis of T cells using high-throughput multiplex amplification and deep sequencing
ActiveUS10202640B2Easy to produceSugar derivativesMicrobiological testing/measurementFunctional studiesOligonucleotide
Methods and oligonucleotide reagents for analyzing individual T cells are disclosed. In particular, the present disclosure provides methods for analyzing individual T cells using high-throughput multiplex amplification and deep sequencing of nucleic acids encoding T cell receptors (TCRs) and various other T cell phenotypic markers. The present disclosure further provides methods of reconstituting TCRs from individual T cells for functional studies, ligand discovery, or screening therapeutics.
Owner:THE BOARD OF TRUSTEES OF THE LELAND STANFORD JUNIOR UNIV
Nanopipette device and method for subcellular analysis
ActiveUS20160032275A1Immobilised enzymesBioreactor/fermenter combinationsChemical treatmentScanning ion-conductance microscopy
Described herein are devices and methods for extracting cellular material from living cells and then depositing them into to a receptacle in a nanoliter scale. Using a nanopipette integrated into a scanning ion conductance microscope (SICM), extraction of mitochondrial DNA from human BJ fibroblasts and Green Fluorescent Protein (GFP) transcripts from HeLa / GFP cells was achieved with minimal disruption to the cellular milieu and without chemical treatment prior to obtaining the isolated sample. Success of the extraction was confirmed by fluorescence microscopy and PCR analysis of the extracted material. The method and apparatus may be applied to many different cell types and intracellular targets, allowing not only single cell analysis, but single subcellular compartment analysis of materials extracted in their native state.
Owner:RGT UNIV OF CALIFORNIA
Digital PCR chip based on seaweed gel liquid drop and application thereof
ActiveCN106754245AImprove stabilityFusion does not occurBioreactor/fermenter combinationsBiological substance pretreatmentsPcr chipCirculating tumor DNA
The invention discloses a liquid drop capturing chip. The liquid drop capturing chip comprises a plurality of fluid micro channels arranged in parallel, wherein a plurality of liquid drop capturing structures are formed along the fluid micro channels at intervals; the liquid drop capturing structures comprise liquid drop entering ends and inhibition liquid drop flowing-out ends; and the inhibition liquid drop flowing-out ends are communicated with the adjacent fluid micro channels. The invention further discloses a fully integrated digital PCR chip based on a seaweed gel liquid drop and application thereof. The digital PCR chip based on the seaweed gel liquid drop, disclosed by the invention, can finish the full process from the generation of liquid drops to capture of the liquid drops and to the detection of the liquid drops. And moreover, seaweed gels are introduced; after the seaweed gels are solidified, the seaweed gel liquid drops are stable, cannot be fused and cannot affect the PCR amplification efficiency; and the method can be applied to the detection of circulating tumor DNA in the blood and other related biology and medical sciences; and due to the introduction of the seaweed gels, the application can be further expanded to single cell analysis and so on.
Owner:SHENZHEN INST OF ADVANCED TECH CHINESE ACAD OF SCI
Fluid-enhanced dielectrophoretic single cell arrangement and control chip and making method thereof
ActiveCN107118938ALow costLow reliabilityBioreactor/fermenter combinationsBiological substance pretreatmentsMicroelectrodeEngineering
The invention belongs to the technical field of biological microfluidic chips. In order to solve the technical problems that the conventional single cell analysis chip is low in reliability, high in cost and difficult in single cell releases, the invention provides a fluid-enhanced dielectrophoretic single cell arrangement and control chip and a making method thereof. The whole chip adopts a sandwich structure, and sequentially comprises conduit connectors, an upper substrate, an upper-layer microelectrode, a microchannel, a microwall array, a lower-layer microelectrode and a lower substrate from top to bottom, wherein two through holes facilitating feeding and discharging of an experimental buffer solution are formed in the upper substrate; the conduit connectors are fixed to the through holes in the upper surface of the upper substrate; both the upper-layer microelectrode and the lower-layer microelectrode are interdigital electrodes; the upper-layer microelectrode and the lower-layer microelectrode are vertically intersected; the microwall array comprises micron-sized circular deep holes; and each microwall is positioned at the center of an intersection of the upper-layer microelectrode and the lower-layer microelectrode. The fluid-enhanced dielectrophoretic single cell arrangement and control chip solves the problems of low efficiency, low reliability, high cost and difficult single cell release, caused by principle singleness, of the conventional chip.
Owner:ZHONGBEI UNIV
Single-cell analysis and authentication method at high flux by combining Raman spectrum with artificial intelligence
The invention discloses a single-cell analysis and authentication method at high flux by combining a Raman spectrum with artificial intelligence, and belongs to the technical field of cell analysis and authentication. The technical scheme of the method comprises the following steps that: firstly, obtaining living body cytosol to be detected, and preparing the cytosol into a single-cell array on achip by a microfluidic technology; then, obtaining the Raman spectrum of the single-cell array, and preprocessing Raman spectrum data; utilizing the preprocessed data to carry out model training, verification and checkout; and finally, obtaining an optimal model, and utilizing the optimal model to carry out authentication prediction on the preprocessed Raman spectrum data. The method has the beneficial effects that the microfluidic technology is used for separating single cells at high flux, the Raman spectrum of the single cell is quickly collected, an artificial intelligence technology is applied to analyze single-cell Raman spectrum characteristics, the single-cell Raman spectrum characteristics are classified and authenticated, authentication accuracy is high, sensitivity is high, andthe problems that the single cell can not be nondestructively and quickly analyzed and authenticated at the high flux at present are solved.
Owner:青岛明德生物科技研究院有限公司
A method for a single cell analysis of telomere length
ActiveUS20160032360A1Accurate measurementHigh resolutionMicrobiological testing/measurementGeneticsCell biology
The invention relates to a method for single cell analysis of relative telomere length using multiplex pre-PCR followed by a qPCR (SCT-pqPCR).
Owner:YALE UNIV +1
Capillary tube electrophoresis chemical luminous detector for monocell analysis
InactiveCN1987432AEasy to manufactureEasy to operateMicrobiological testing/measurementChemiluminescene/bioluminescenceCapillary electrophoresisElectrophoresis
The detection device includes slide glass with transparent film attached on surface, reactor, separation capillary (SC), reaction capillary with detection window (DW), luminescence detection part located at DW, and micromanipulator. Being positioned between the slide glass and the reactor, the micromanipulator is in use for grasping SC. Cell suspension pool and dissolve film pool are setup on the transparent film. Being setup on the slide glass, buffer solution pool is connected to high-voltage end. The reactor includes reaction continuous groove (RCG), and inlet connected to RCG for adding chemiluminescence reagent. RCG is connected to ground. RCG is connected to the reaction capillary. SC is setup in RCG. One end of SC is on the transparent film, and the other end is positioned at DW. Features are: easy of preparation, and operation, small size, low cost, and high detection sensitivity. The detection device is suitable for simple cell analysis, and research in area of life sciences.
Owner:WUHAN UNIV
Microfluid control system for separating, focusing and sorting of rare cells and application
ActiveCN110157609AEasy to separateAchieve separationBioreactor/fermenter combinationsBiological substance pretreatmentsDiseaseControl system
The invention relates to the technical field of detection of rare cells, in particular to a microfluid control system for separating, focusing and sorting of rare cells and application. A microfluid control chip device comprising a separating unit, a focusing unit, a sorting unit and a flow resistance matching unit serves as the core of the system. The system is characterized in that the system can integrate operation like separation, focusing, single-cell analysis and sorting on the rare cells in body fluid such as blood in a continuous mode on one chip for completion, and therefore, the system can be widely applied to separation and sorting of the rare cells in the body fluid, and disease diagnosis, drug screening, individual-based treatment and other fields based on the rare cells.
Owner:SHANDONG NORMAL UNIV
Single cell analysis of HIV replication capacity and drug resistance
ActiveUS20050244818A1Less-effective in controllingAccurate countVectorsMicrobiological testing/measurementAssayResistant virus
A novel single-cell-level phenotypic assay is described, which can simultaneously analyze HIV-1 drug susceptibility and intrinsic replication capacity. This allows quantitative dissection of the functions of antiretroviral drugs into suppression of viral replication and selection of resistant viruses with diminished replication capacities. The disclosed assay provides a tool for the rational evaluation of treatment decisions for patients failing antiretroviral therapy and is expected to be an important part in clinical management of HIV.
Owner:THE JOHN HOPKINS UNIV SCHOOL OF MEDICINE
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