Capillary tube electrophoresis chemical luminous detector for monocell analysis

A technology of chemiluminescence detection and capillary electrophoresis, which is applied in the field of analytical instruments, can solve the problems of laser excitation wavelength limitation, complex LIF optical structure, interference analysis component determination, etc., and achieve the effect of small size, high detection sensitivity and convenient operation

Inactive Publication Date: 2007-06-27
WUHAN UNIV
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  • Abstract
  • Description
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  • Application Information

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Problems solved by technology

At present, the problems of both LIF and mass spectrometry are that they are expensive and the size of the detector is too large, while LIF also has disadvantages such as complex optical structure and limitation by the excitation waveleng

Method used

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  • Capillary tube electrophoresis chemical luminous detector for monocell analysis
  • Capillary tube electrophoresis chemical luminous detector for monocell analysis

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Experimental program
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Embodiment 1

[0017] Fig. 3 is the capillary electrophoresis chemiluminescence spectrum for detecting single human red blood cells in the present invention. Experimental conditions: 60cm long, 15 micron inner diameter quartz capillary, 30mM phosphate buffer (pH9.0), separation voltage 16kV. As shown, HemA is hemoglobin. The device of the invention can be used to detect the hemoglobin in a human single red blood cell within about 10 minutes, reflecting the characteristics of the device being convenient and highly sensitive.

Embodiment 2

[0019] Fig. 4 is a capillary electrophoresis chemiluminescence spectrum for detecting a single human red blood cell according to the present invention. Experimental conditions: 57cm long, 25 micron inner diameter quartz capillary, 30mM borate buffer (pH9.0), SDS 0.5mM, separation voltage 16kV. As shown in the figure, HemA is hemoglobin, and a and b are unknown peaks. The device of the present invention can also detect other components in human red blood cells, which facilitates the analysis of cell components.

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Abstract

The detection device includes slide glass with transparent film attached on surface, reactor, separation capillary (SC), reaction capillary with detection window (DW), luminescence detection part located at DW, and micromanipulator. Being positioned between the slide glass and the reactor, the micromanipulator is in use for grasping SC. Cell suspension pool and dissolve film pool are setup on the transparent film. Being setup on the slide glass, buffer solution pool is connected to high-voltage end. The reactor includes reaction continuous groove (RCG), and inlet connected to RCG for adding chemiluminescence reagent. RCG is connected to ground. RCG is connected to the reaction capillary. SC is setup in RCG. One end of SC is on the transparent film, and the other end is positioned at DW. Features are: easy of preparation, and operation, small size, low cost, and high detection sensitivity. The detection device is suitable for simple cell analysis, and research in area of life sciences.

Description

technical field [0001] The invention relates to an analysis instrument, in particular to a capillary electrophoresis chemiluminescence detection device for cell analysis. Background technique [0002] Cells are the fundamental units of life, and single-cell analysis may answer long-standing questions in life sciences and biomedicine. Cells are small in size (fL-nL), low in chemical components (zmol-fmol), and complex in composition. These characteristics require that single-cell analysis techniques should have the characteristics of small sampling, high sensitivity, strong separation ability, and rapidity. Capillary electrophoresis is a high-efficiency separation method, which is characterized by rapidity, low sample consumption, and high sensitivity, making it compatible with single-cell analysis techniques. At present, the detection methods for single-cell analysis by capillary electrophoresis are mainly laser-induced fluorescence (Laser Induced Fluorescence, LIF), electr...

Claims

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Application Information

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IPC IPC(8): G01N21/76G01N27/447C12Q1/02
Inventor 时宝宪黄卫华程介克
Owner WUHAN UNIV
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