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Preparation method of surface-modified fluorescent magnetic polymer composite microspheres

A technology of composite microspheres and surface modification, which is applied across the three major fields of chemistry, physics and biology

Inactive Publication Date: 2013-01-09
SHAANXI NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0007] At present, the preparation technology of fluorescent microspheres in China has just started, and there are few reports on the preparation technology of high-performance fluorescent magnetic composite microspheres. The present invention has successfully prepared surface-modified fluorescent magnetic polymer composite microspheres.

Method used

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  • Preparation method of surface-modified fluorescent magnetic polymer composite microspheres
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  • Preparation method of surface-modified fluorescent magnetic polymer composite microspheres

Examples

Experimental program
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Effect test

Embodiment 1

[0047] The steps of the preparation method of the surface-modified fluorescent magnetic polymer composite microspheres in this example are as follows:

[0048] 1. Preparation of dextran-modified nanoscale Fe 3 o 4 Dispersions

[0049] 20°C, add 150mL twice distilled water into the three-necked flask, stir and add 2.93g FeCl in turn under the protection of N2 3 ·6H 2 O was formulated into FeCl with a mass concentration of 19.55% 3 ·6H 2 O aqueous solution, 1.45g FeCl 2 4H 2 O was formulated into FeCl with a mass concentration of 14.46% 2 4H 2 O aqueous solution, 1g number-average molecular weight is that the dextran of 40000 is mixed with mass concentration and is that 3.23% number-average molecular weight is the dextran aqueous solution of 40000, stirring fully mixes, and adding 2.20g NaOH is mixed with the NaOH aqueous solution that mass concentration is 3.92%, Dextran and FeCl 3 ·6H 2 O, FeCl 24H 2 The mass ratio of O, NaOH, and double-distilled water is 1:2.93:...

Embodiment 2

[0065] The steps of the preparation method of the surface-modified fluorescent magnetic polymer composite microspheres in this example are as follows:

[0066] In the preparation of dextran-modified nanoscale Fe 3 o 4 In step 1 of the dispersion solution, add 150 mL of twice distilled water into a three-necked flask at 20°C, and stir under N 2 Under protection, add 2.93g FeCl successively 3 ·6H 2 O was formulated into FeCl with a mass concentration of 19.55% 3 ·6H 2 O aqueous solution, 1.45g FeCl 2 4H 2 O was formulated into FeCl with a mass concentration of 14.46% 2 4H 2 O aqueous solution, 1g number-average molecular weight is that the dextran of 40000 is mixed with mass concentration and is that 3.23% number-average molecular weight is the dextran aqueous solution of 40000, stirring fully mixes, and adding 2.20g NaOH is mixed with the NaOH aqueous solution that mass concentration is 3.92%, Dextran and FeCl 3 ·6H 2 O, FeCl 2 4H 2 The mass ratio of O, NaOH, and d...

Embodiment 3

[0073] The steps of the preparation method of the surface-modified fluorescent magnetic polymer composite microspheres in this example are as follows:

[0074] In the preparation of dextran-modified nanoscale Fe 3 o 4 In step 1 of the dispersion solution, add 150 mL of twice distilled water into a three-necked flask at 20°C, and stir under N 2 Under protection, add 2.93g FeCl successively 3 ·6H 2 O was formulated into FeCl with a mass concentration of 19.55% 3 ·6H 2 O aqueous solution, 1.45g FeCl 2 4H 2 O was formulated into FeCl with a mass concentration of 14.46% 2 4H 2 O aqueous solution, 1g number-average molecular weight is that the dextran of 40000 is mixed with mass concentration and is that 3.23% number-average molecular weight is the dextran aqueous solution of 40000, stirring fully mixes, and adding 2.20g NaOH is mixed with the NaOH aqueous solution that mass concentration is 3.92% , dextran and FeCl 3 ·6H 2 O, FeCl 2 4H 2 The mass ratio of O, NaOH, and ...

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Abstract

The invention provides a preparation method of surface-modified fluorescent magnetic polymer composite microspheres. The method comprises the steps of preparing a dextran-modified nano-scale Fe3O4 dispersion liquid, preparing a mixed surfactant, preparing an oil phase, preparing an emulsion, preparing an aqueous phase, preparing a magnetic nano-scale Fe3O4 copolymer composite micro-gel, preparing SiO2-coated nano-scale Fe3O4 copolymerized magnetic polymer composite microspheres, and preparing the surface-modified fluorescent magnetic polymer composite microspheres. The prepared surface-modified fluorescent magnetic polymer composite microspheres are tested by using a fluorescence microscope, a scanning electron microscope, a fluorescence spectrometer, and a vibrating sample magnetometer; the test results show that the microspheres have the characteristics of high fluorescent luminous emissivity, good magnetic responsibility and stable performance, the microspheres have the rigidity and stability of inorganic materials and the flexibility of organic materials, and the microspheres can be applied to the aspects of immunoassay, drug carriers, immobilized enzyme, single nucleotide polymorphism genotype, cytokine identification, single-cell analysis and the like.

Description

technical field [0001] The invention spans three technical fields of chemistry, physics and biology, and specifically relates to colloid chemistry, fluorescent substances, magnetic particles and the like. For example: preparation of microcapsules, colloidal materials and their solutions not included in other categories, preparation of microgels or fluorescent magnetic multifunctional composite microspheres. Background technique [0002] Functional microspheres are characterized by their inherent large specific surface, strong adsorption capacity, large agglutination and strong surface reaction ability, as well as various properties given by people according to different uses, such as biospecific binding, hollow porosity, Magnetic separation has been widely used. [0003] Fluorescent microspheres generally refer to microspheres that are bound with fluorescent substances (including surface coating) on ​​the surface of the microspheres or microspheres that contain fluorescent ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B01J13/02C09K11/06C09K11/02H01F1/42
Inventor 王公正莫润阳贺亚妮房喻
Owner SHAANXI NORMAL UNIV
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