123 results about "Native state" patented technology

Preferred embodiments of the present invention are directed to systems for phase measurement which address the problem of phase noise using combinations of a number of strategies including, but not limited to, common-path interferometry, phase referencing, active stabilization and differential measurement. Embodiment are directed to optical devices for imaging small biological objects with light. These embodiments can be applied to the fields of, for example, cellular physiology and neuroscience. These preferred embodiments are based on principles of phase measurements and imaging technologies. The scientific motivation for using phase measurements and imaging technologies is derived from, for example, cellular biology at the sub-micron level which can include, without limitation, imaging origins of dysplasia, cellular communication, neuronal transmission and implementation of the genetic code. The structure and dynamics of sub-cellular constituents cannot be currently studied in their native state using the existing methods and technologies including, for example, x-ray and neutron scattering. In contrast, light based techniques with nanometer resolution enable the cellular machinery to be studied in its native state. Thus, preferred embodiments of the present invention include systems based on principles of interferometry and/or phase measurements and are used to study cellular physiology. These systems include principles of low coherence interferometry (LCI) using optical interferometers to measure phase, or light scattering spectroscopy (LSS) wherein interference within the cellular components themselves is used, or in the alternative the principles of LCI and LSS can be combined to result in systems of the present invention.

Preferred embodiments of the present invention are directed to systems for phase measurement which address the problem of phase noise using combinations of a number of strategies including, but not limited to, common-path interferometry, phase referencing, active stabilization and differential measurement. Embodiment are directed to optical devices for imaging small biological objects with light. These embodiments can be applied to the fields of, for example, cellular physiology and neuroscience. These preferred embodiments are based on principles of phase measurements and imaging technologies. The scientific motivation for using phase measurements and imaging technologies is derived from, for example, cellular biology at the sub-micron level which can include, without limitation, imaging origins of dysplasia, cellular communication, neuronal transmission and implementation of the genetic code. The structure and dynamics of sub-cellular constituents cannot be currently studied in their native state using the existing methods and technologies including, for example, x-ray and neutron scattering. In contrast, light based techniques with nanometer resolution enable the cellular machinery to be studied in its native state. Thus, preferred embodiments of the present invention include systems based on principles of interferometry and/or phase measurements and are used to study cellular physiology. These systems include principles of low coherence interferometry (LCI) using optical interferometers to measure phase, or light scattering spectroscopy (LSS) wherein interference within the cellular components themselves is used, or in the alternative the principles of LCI and LSS can be combined to result in systems of the present invention.

Elucidating the function of proteins in mammalian cells is particularly challenging due to the inherent complexity of these systems. Methods to study protein function in living cells ideally perturb the activity of only the protein of interest but otherwise maintain the natural state of the host cell or organism. Ligand-dependent inteins offer single-protein specificity and other desirable features as an approach to control protein function in cells post-translationally. Some aspects of this invention provide second-generation ligand-dependent inteins that splice to substantially higher yields and with faster kinetics in the presence of the cell-permeable small molecule 4-HT, especially at 37° C., while exhibiting comparable or improved low levels of background splicing in the absence of 4-HT, as compared to the parental inteins. These improvements were observed in four protein contexts tested in mammalian cells at 37° C., as well as in yeast cells assayed at 30° C. or 37° C. The newly evolved inteins described herein are therefore promising tools as conditional modulators of protein structure and function in yeast and mammalian cells.

Natural fiber welding is a process by which individual fibers are swollen by an appropriate ionic liquid-based solvent system to form a congealed network. Manipulated fibrous materials may be either composed of natural polymers such as cellulose, hemicellulose, silk, et cetera, or synthetic polymers, or mixed materials. The process is principally controlled by the composition of the solvent system which includes an ionic liquid solvent plus additives such as water, methanol, et cetera. Other conditions such as the amount and placement of solvent, as well as time, temperature, and pressure control the extent to which neighboring fibers are fused. Only the material at the outer surface of fibers need be sufficiently mobile to merge with that of neighboring fibers. Material in the fiber core may be left in the native state by controlling process variables. Fibers form a congealed network upon removal of the ionic liquid-based solvent.

A photoactive moiety exhibiting an anisotropic transition dipole. The moiety exhibits emission of polarized light in response to energy absorption. In a preferred embodiment, the moiety comprises a particle from the group consisting of a crystalline arrangement of photoactive molecules and a photoactive nanocrystal. The moiety may include a matrix in which photoactive objects exhibiting an anisotropic emission dipole are embedded. The moiety may be photobleached to product the anisotropy and the photoactive objects may have a one dimensional transition dipole in their natural state.

To store input data in a plurality of memory cells, a mapping function of bit sequences to physical parameter states of the cells is provided. The cells are programmed, in accordance with the mapping function, to store the input data, in a way that would store uniformly distributed data with a programming state distribution other than any native state distribution of the mapping function. To store input data in a single memory cell, a mapping function of bit sequences to states of a physical parameter of the cell, such that if uniformly distributed data were stored in a plurality of such memory cells then the states of the physical parameter of the cells would be distributed non-uniformly, is provided. The memory cell is programmed to store the input data in accordance with the mapping function.

Preferred embodiments of the present invention are directed to systems for phase measurement which address the problem of phase noise using combinations of a number of strategies including, but not limited to, common-path interferometry, phase referencing, active stabilization and differential measurement. Embodiment are directed to optical devices for imaging small biological objects with light. These embodiments can be applied to the fields of, for example, cellular physiology and neuroscience. These preferred embodiments are based on principles of phase measurements and imaging technologies. The scientific motivation for using phase measurements and imaging technologies is derived from, for example, cellular biology at the sub-micron level which can include, without limitation, imaging origins of dysplasia, cellular communication, neuronal transmission and implementation of the genetic code. The structure and dynamics of sub-cellular constituents cannot be currently studied in their native state using the existing methods and technologies including, for example, x-ray and neutron scattering. In contrast, light based techniques with nanometer resolution enable the cellular machinery to be studied in its native state. Thus, preferred embodiments of the present invention include systems based on principles of interferometry and/or phase measurements and are used to study cellular physiology. These systems include principles of low coherence interferometry (LCI) using optical interferometers to measure phase, or light scattering spectroscopy (LSS) wherein interference within the cellular components themselves is used, or in the alternative the principles of LCI and LSS can be combined to result in systems of the present invention.

This invention relates directly to the display of deer antlers, as a sculpture, replicating the look of a “European” style mount—a natural, boiled and bleached skull. This invention resembles that of a natural buck's skull—complete with all those characteristics of a skull in its natural state. The mount consists of two steel plates. The outermost plate in the shape and possessing characteristics of a deer skull, the back plate, flat, having slots and protrusions to enable the user to hang on the wall or bracket in a three-dimensional manner. This invention is for the sole purpose of decoration and display of a deer's antlers.

The present disclosure relates generally to multi-specific Fab fusion proteins (MSFP) which comprise an antibody Fab fragment with both N-termini fused to a fusion moiety (fusion moiety A or B). MSFP containing the Fab fragment exhibit significantly reduced binding ability of the Fab fragment to the Fab target. Binding of the Fab to its target is restored when the MSFP is clustered on a cell surface by binding of the fusion moieties to their target. The reduced binding of the Fab to its target, especially when presented on a cell surface in its native state, absent fusion moiety binding provides advantages such as: reduced side effects and allows desirable pharmacological effects of selectivity and specificity in a controlled manner.

Disclosed are methods and compositions for enriching nucleic acid fragments from a sample that include one or more target region of interest. In certain aspects, a sample of double stranded nucleic acid fragments having a strand-linking adapter at one end and a non-strand-linking adapter at the other end are denatured and contacted with capture probes specific for a target sequence of interest. Capture probe-bound fragments are isolated from the sample, e.g., using a solid substrate specific for the binding moiety on the capture probes, and are renatured for downstream processing, thus maintaining the original double-stranded region. This enrichment process does not require amplification and as such maintains the nucleic acids in their native states. The disclosed enrichment process and compositions are suitable for analyzing nucleic acids that are fragmented and/or damaged, e.g., cell-free DNA such as circulating tumor DNA, as well as nucleic acids that are many kilobases in length.

An apparatus for allowing IEEE-1394-enabled devices to communicate with one another across an existing coaxial cable network in, for example, a house. Many houses have been “wired for cable” and have such a cable network in place. The network is, in its native state, unsuitable for IEEE-1394 communication. The apparatus allows processing signals from an IEEE-1394-enabled device for transmission across a coax. Signals received are converted and then provided to IEEE-1394 devices connected to the apparatus. One use for the apparatus is interconnecting audio/visual devices, for example high definition television receivers, DVD players, audio components and the like located in throughout a house to one another. DTCP encryption and decryption may be provided. The apparatus may be equipped with an optional SMI connector to allow communication over an optical fiber in lieu of the coaxial network. The apparatus is self-powered, deriving necessary power from the IEEE-1394 interface itself.

The invention belongs to the aquaculture technology field, in particular relating to an artificial control method for the improvement of female finless eel parent fecundity. The finless eel has special physiological phenomena and sex reversal characteristics with small size, large egg granules and low fecundity. Thus the scale finless eel propagation is subject to the restrictions of the female finless eel parent self-conditions. In the invention, through the artificial simulation of female finless eel culture and development environment, the rational selection of parental finless eels, the reasonable stocking density arrangement, the appropriate feeding and management methods, the pre-oviposition period intensive culture and the artificial control measures, the female finless eel fecundity in natural state can be enhanced to the fecundity up to 450 to 1300 grains each and the 680 grains average level from 170 to 650 grains each and the average 260 grains. Therefore, the average fecundity of finless eel is improved by 161%.

The invention discloses a protein structure prediction method based on tree structure replica exchange and fragment assembly. The method includes the following steps that protein conformations are based on a replica exchange thought, on the basis that preservation of a plurality of replica messages is facilitated, protein conformation space search can be enhanced, the advantage of a tree data structure is used, energy layering and protein middle conformation shape information can also be used, a specific weight function is used for selecting the entire protein confirmation spaces through certain probability as compiled replicas, the protein conformations are led to step towards a low-energy path, the diversity of the protein conformations can be reserved to the greatest extent, and thus the purpose for predicting the protein native state can be achieved ultimately. By means of the protein structure prediction method based on tree structure replica exchange and fragment assembly, the calculated amount can be greatly reduced, computing time is shortened, and meanwhile it is guaranteed that the low-energy conformations can be searched for.

The present invention provides an agglutinant protein obtained by separating plant astragalus root and purifying its extract and its preparation method. In the concrete, said invention uses the astragalus root as raw material, and utilizes ammonium sulfate with different saturations to make fractional precipitation and multistep ion exchange column chromatography so as to obtain astragalus agglutinant protein which can be used for blood agglutination and has the obvious antifungal action.

The invention relates to a rock test piece which comprises a cylindrical main body, wherein at least one water-guiding blind hole is respectively processed on each of the two end faces of the cylindrical main body. A jointed rock mass test piece is obtained by performing a rock forming simulation test on the rock test piece. According to the rock forming simulation test, a tri-axial compression whole-process test is performed on each rock test piece under the condition of applying a confining pressure, the rock test piece is crushed, and communicated influent channels are formed by a crushed surface of the rock test piece and the water-guiding blind hole. A jointed rock mass and aqueous rock coupling test method comprises the following steps: (1) collecting the rocks in a to-be-constructed engineering rock mass and processing the rocks into the rock test pieces with such shape and structure, and (2) mounting a set of rock test pieces on rock mechanic test equipment, and performing a rock forming simulation test, a stress adjusting test, a natural state test and an aqueous rock coupling test in turn, thereby obtaining a quantitative description for the influence of water pressure on the mechanical parameters of the to-be-constructed engineering rock mass.

The invention relates to a honey peach preservative and an application method thereof; the honey peach preservative comprises the following components according to corresponding parts by weight: 4-5 parts of chitosan of 600, 000 molecular weight, 0.5-0.6 part of sodium benzoate, 1-1.2 parts of sodium polyacrylate, 2-2.2 parts of organic acid and remainder of water; the application method of the honey peach preservative comprises the steps of batching, spraying and drying. By coating preservative on the surface of honey peach, the invention reasonably utilizes filming property, inoxidizability, bacteriostasis and sterilization effects and synergy effect of natural state biological molecules, so that the freshness time of honey peach can be greatly prolonged, honey peach can be stored for a long term and transported for a long distance without going bad in a short time, and the storage cost in the transporting process is reduced, thus being favourable for storage by customers and ensuring the benefits of fruit growers and consumers with low cost.

A high sand content bore simulation testing method relates to a testing method for simulating high sand content bore in a river mouth model test, particularly relates to a method and a device for simulating the bore head sand content during the bore and belongs to the field of coastal engineering model tests. A test water tank device for simulating the high sand content bore and a method for utilizing the device to conduct simulation test are further provided. The test water tank device comprises a variable water head high level water tank, a test water tank and an underground reservoir. A tail door and the variable water head high level water tank are utilized to adjust the flowing speed of water in the water tank to reach the preset value, a fast gate is closed, the water flow is stopped and generates the bore in the water tank, the bore upwells upstream from the gate and drives sand in a sand tank, and vertical sand distribution in the water tank is measured at the moment to obtain the natural-state vertical sand distribution characteristics. The device and the method specially used for simulating the vertical sand distribution of the bore are provided. The sand distribution obtained by the method is similar to the natural sand distribution. The device meets the law of similarity.

The invention discloses a shore/beach sediment release simulation experiment device and a shore/beach sediment simulation experiment method. The shore/beach sediment release simulation experiment device comprises a wave generating device, a measuring device and a water level fluctuating device, wherein the water level fluctuating device comprises a slope variable water channel the slope of which can be regulated, and a lifting barrel which is connected with the slope variable water channel by virtue of a water outlet pipe and can be lifted in height; the wave generating device is arranged in the slope variable water channel, and comprises a rotating disc sliding groove formed vertical to the bottom of the slope variable water channel, a rotating disc which is arranged on the rotating disc sliding groove in a sliding manner and can rotate, and a floating body connected with the rotating disc. A mud groove is formed in the slope variable water channel. Water is injected in the lifting barrel and the water level fluctuation time and the fluctuation period in the slope variable water channel and the water level fluctuation are controlled, the rotating disc is rotated to continuously generate wave, so that the bottom permeability of bottom mud in a natural state and the fluctuation characteristic close to the natural water level can be restored, and the problem of experiment research of bottom mud pollution release under a hydrodynamic action that a lake shore is periodically flooded and repeatedly scoured by water can be simulated.

Methods for transporting a biologically active agent across a cellular membrane or a lipid bilayer. A first method includes the steps of:

• • (a) providing a biologically active agent which can exist in a native conformational state, a denatured conformational state, and an intermediate conformational state which is reversible to the native state and which is conformationally between the native and denatured states;
  • (b) exposing the biologically active agent to a complexing perturbant to reversibly transform the biologically active agent to the intermediate state and to form a transportable supramolecular complex; and
  • (c) exposing the membrane or bilayer to the supramolecular complex, to transport the biologically active agent across the membrane or bilayer. The perturbant has a molecular weight between about 150 and about 600 daltons, and contains at least one hydrophilic moiety and at least one hydrophobic moiety. The supramolecular complex comprises the perturbant non-covalently bound or complexed with the biologically active agent. In the present invention, the biologically active agent does not form a microsphere after interacting with the perturbant. A method for preparing an orally administrable biologically active agent comprising steps (a) and (b) above is also provided as are oral delivery compositions.

Additionally, mimetics and methods for preparing mimetics are contemplated.

This invention discloses a new technique for extracting high molecular weight chitin. This invention utilizes shrimp shell as the raw material, and adopts citric acid decalcification technique to obtain chitin, which does no harm to the molecular chain of chitin, and can keep the natural state of chitin. Compared with the present HCl and EDTA decalcification techniques, this new technique can obtain high molecular weight (higher than 180000) chitin with a yield increased by more than 12%.