Method and apparatus for detecting bacterial resistance by single cell analysis

A drug-resistant, single-cell technology, applied in the field of physics, can solve the problems of complex sample preparation methods, time-consuming and easy-to-interfere testing of bacterial drug resistance, and achieve the effects of fast measurement, rapid disease diagnosis, and simple processing.

Active Publication Date: 2019-01-25
上海镭立激光科技有限公司
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  • Abstract
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  • Application Information

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Problems solved by technology

[0019] The object of the present invention is to provide a method and device for detecting bacterial drug resistance through single-cell analysis. The method and device for detecting bacterial drug resistance through single-cell an

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  • Method and apparatus for detecting bacterial resistance by single cell analysis
  • Method and apparatus for detecting bacterial resistance by single cell analysis
  • Method and apparatus for detecting bacterial resistance by single cell analysis

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Embodiment 1

[0050] like figure 1 As shown, the present invention provides a device for detecting bacterial drug resistance through single-cell analysis, including a plate-type micro-biochemical reaction system, and the plate-type micro-biochemical reaction system is provided with a slide glass 53 and a cover glass 51, and The sealing agent 52 is used to seal the surroundings, the plate type micro-biochemical reaction system is placed on the stage 14, and the motor 61 is connected to the stage 14 to drive the stage 14 to move relatively.

[0051] It also includes a Raman spectrum excitation system, in which a Raman spectrum excitation light source 41, a first beam shaper 42, a laser scanning device 43, A second beam shaper 44 , a third beam splitter 33 , a second beam splitter 24 and an objective lens 25 .

[0052] Specifically, the laser scanning device 43 is connected to a second beam shaper 44, and the laser scanning device 43 and the second beam shaper 44 are arranged on the 45-degree...

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Abstract

A method for detect bacterial drug resistance by single cell analysis comprises mixing a certain amount of bacterial sample and a certain amount of antibiotic diluent on a slide, covering that slide with a cover glass and sealing with a sealant on four side to form a plate-type microbiochemical reaction system in which the bacterial and antibiotic diluent react biochemically. Bacteria were observed and located by phase contrast microscope and Raman spectra were obtained by laser scanning. Bacterial drug resistance could be obtained by analysis. The invention also provides a device for realizing the method, and the device comprising a plate-type micro-biochemical reaction system, a phase contrast microscope, a high-power optical microscope, a Raman spectrum excitation system and a Raman spectrum collection system. Bacteria were observed by phase contrast microscope and high power optical microscope, and then Raman spectra were obtained by Raman spectrum excitation system and Raman spectrum collection system to detect bacterial drug resistance.

Description

technical field [0001] The invention relates to the field of physics, in particular to optical detection technology, in particular to a method and device for detecting bacterial drug resistance through single-cell analysis. Background technique [0002] According to a comprehensive analysis of various data by relevant professors from Zhejiang University School of Medicine, it is found that the total annual consumption of antibiotics in my country is about 150,000 to 200,000 tons, accounting for about 50% of the world's total usage. At the same time, due to factors such as dense population, distribution of pharmaceutical industry, agriculture, and breeding industry, the antibiotic use and discharge density in eastern China's watersheds is more than 6 times higher than that in western watersheds. Massive and often unnecessary antibiotic use means that an increasing number of pathogens are drug-insensitive, and previously treatable infections can become life-threatening. [00...

Claims

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Application Information

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IPC IPC(8): C12Q1/18G01N21/45G01N21/65C12M1/34C12R1/19
CPCG01N21/45G01N21/65C12Q1/18
Inventor 倪勤朱宝强苏江文龚辉
Owner 上海镭立激光科技有限公司
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