Full-automatic single particle/single cell capturing chip based on digital microfluids technique and application thereof

Abstract

The invention discloses a full-automatic single particle/single cell capturing chip based on a digital microfluids technique and application thereof. The full-automatic single particle/single cell capturing chip comprises a digital microfluids chip with multiple pattern layers and an integrated circuit, wherein the digital microfluids chip with multiple pattern layers consists of an upper electrode plate and a lower electrode plate; the upper electrode plate adopts lyophobic conductive glass as a ground electrode; the integrated circuit is used as a digital microfluids circuit control system.The full-automatic single particle/single cell capturing chip has the advantages that the single particles/single cells can be fully automatically captured, the operation is simple and quick, and thecapturing efficiency is high; a sample can be recycled without damage, and the capturing chip is especially suitable for rare samples; the continuous and complicated parallel operation of the capturing unit is realized by program control, and the crossing pollution is avoided; the capturing chip can be widely applied to the fields of detection of single particles and analysis of single cells, suchas isolation, coupling and detection of single particles, and isolation, culture and nucleic acid amplification of single cells.

Description

technical field [0001] The invention belongs to the technical field of digital microfluidics, in particular to a fully automatic single particle / single cell capture chip based on digital microfluidics technology and its application. Background technique [0002] As the basic unit of the structure and function of living organisms, cells are an important basis for the study of the laws of life activities. The current life science research mainly takes a large number of cells as the research object, and adopts the analysis method of the cell population to obtain the chemical information in the cells. However, there are significant microscopic inhomogeneities (heterogeneity) among different individual cells. The statistical analysis results based on a large number of cells will cover up the differences between single cells, and it is difficult to reflect the rules of life activities at the single cell level, resulting in In addition, medicine, biology and other disciplines are ...

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Problems solved by technology

However, the limitations of conventional microfluidic chips are also very obvious. It requires the use of mechanical pumps and valves, which makes integration difficult, and it is difficult to achieve continuous multi-step processing of samples; it is difficult to accurately control the position of multiple reagents and reaction time; And it is impossible to achieve single-cell capture of a small number of rare cells, and it is even more powerless in the field of high-efficiency and high-throughput analysis of a large number of single cells

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