Apparatus for forming and screening two-dimensional liquid droplet array, and use method thereof

A droplet and array technology, applied in the direction of analysis materials, instruments, etc., can solve the problems of slow droplet generation, complicated operation of droplet array, limited application, etc., and achieve the effect of automatic detection and automatic absorption
CN106568982AActive Publication Date: 2017-04-19ZHEJIANG UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
ZHEJIANG UNIV
Publication Date
2017-04-19

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Abstract

The present invention discloses an apparatus for forming and screening a large-scale single-layer two-dimensional liquid droplet array, and a use method thereof. The apparatus comprises a micro-column array chip, a scraping plate for auxiliary spreading of a liquid droplet, a probe for controlling the liquid droplet, a three-dimensional translation table and other components, wherein a micro groove unit for trapping the single liquid droplet is formed by using the gap between micro-columns, and the liquid droplet is spread in the micro groove unit in a single-layer and single manner through gravity effect and capillary effect or through an auxiliary means so as to form the single-layer liquid droplet array. With the apparatus of the present invention, the accurate positioning and detection on the liquid droplet, the sucking on the target liquid droplet and the subsequent treatments can be performed. According to the present invention, the apparatus has characteristics of simple structure and easy operation; and the method has advantages of fast liquid droplet spreading, high flux, high liquid droplet trapping efficiency, liquid droplet position fixing and the like, and is suitable for single cell analysis and screening, single molecule analysis and screening, high-throughput gene screening, protein directed evolution, antibody screening, microbial research, drug screening, and other fields.
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Description

technical field

[0001] The field of the invention is the field of microfluidic liquid droplets, in particular to a device for forming and screening a large-scale two-dimensional droplet array and a method for using the same. Background technique

[0002] Droplet-based multiphase microfluidic technology is one of the hotspots in the field of high-throughput screening research in recent years. It realizes the generation, mixing, reaction, and analysis and screening of large quantities of water-in-oil or oil-in-water droplet microreactors through the control of multiphase fluids in micron-scale channels (or microstructures). Droplet-based multiphase microfluidic technology solves the problems of slow mixing speed, low analysis throughput, and easy cross-contamination in single-phase microfluidic systems, and further reduces the consumption of reagents and samples. In addition, micro-droplet technology has the advantages of automated operation, high generation frequency, and fa...

Claims

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