7 results about "Sodium fluorescein" patented technology

The invention relates to the technical field of intelligent sensing, in particular to a method for measuring an underground water migration path by utilizing a tracer agent, which comprises the following steps: synchronously acquiring two paths of signals according to the action of pump light and probe light on a fluorescein sodium tracer agent in a pore medium, and the returned stimulated light and the local oscillation light subjected to frequency shift by the acousto-optic modulator are interfered to generate an interference beat frequency electric signal. Two different optical signals from the same tracer block mass are synchronously collected and deeply analyzed, multi-dimensional and high-precision representation of the underground water migration process is achieved, returned stimulated emission light is interfered with local light, spectrum analysis is carried out on interference beat frequency signals, and the accuracy of the underground water migration process is improved. The instantaneous flow velocity value reflecting the motion state of the tracer agent block mass can be obtained, the limitation that the flow velocity is calculated by depending on the average time between two points traditionally is avoided, and the real dynamic change rate of the fluid in the complex pore medium can be captured.

The invention discloses a preparation method and application of a high-purity low-concentration fluorescein sodium aqueous solution. The preparation method comprises the steps of raw material pretreatment, solution preparation and stability optimization, wherein the raw material pretreatment comprises the following steps: synthesizing fluorescein from phthalic anhydride and resorcinol under a solvent-free condition; then purifying fluorescein through a diacetylation-saponification-salifying process, and controlling the pH value to 8.0 to improve the salifying efficiency; the solution preparation comprises the following steps: dissolving high-purity fluorescein sodium (the purity is greater than or equal to 99.5%) in water for injection, reducing the risk of thermal degradation by adopting a low-temperature gradient dissolution method, accurately controlling the concentration to 1-6% (w / v), and verifying the clarity of the solution by adopting an ultraviolet-visible spectrophotometric method. The total amount of impurities is less than or equal to 0.1% by combining activated carbon adsorption (0.1% dosage, reflux for 30 minutes) and acid-base crystallization (crystallization at pH of 1-2 and redissolution at pH of 8.5-9.0); through a buffer system with pH of 8.0-9.0 and low-temperature operation, the solution is stored for 6 months in a dark place at 4 DEG C, and the content decrease rate is less than or equal to 1.0%.

The invention discloses a saliva flow rate detection strip for auxiliary diagnosis of xerostomia and a detection method thereof. The detection strip comprises strip-shaped filter paper pretreated by fluorescein sodium, the filter paper is packaged in a food-grade plastic shell, one end of the filter paper extends out of the shell to form a 0.5 cm sampling part, and scale marks are arranged on the filter paper. During detection, the sampling part is placed under the tongue for 3 minutes, saliva infiltrates along the filter paper under the capillary action and dissolves the fluorescein sodium for color development, and the flow rate of the unstimulated saliva can be obtained through conversion by reading the infiltration length. The detection strip can effectively distinguish a control person from a sicca syndrome patient, a xerostomia patient and the like. The problems that a traditional method is long in consumed time, poor in experience and inconvenient to operate are solved, and a detection tool which is rapid, objective and standardized and is in seamless butt joint with an existing diagnostic standard is provided.

This invention discloses a dual-emission fluorescent nano-assembly, its preparation method, and its application in ATP detection. The co-assembly achieves the detection of adenosine triphosphate (ATP). This invention uses an amphiphilic TPE derivative as the energy donor and an amphiphilic imidazole compound as the anion acceptor to form a dual-emission fluorescent nano-assembly with sodium fluorescein. Significant energy transfer occurs, resulting in strong fluorescence from the sodium fluorescein. After sodium fluorescein is replaced by ATP, the donor regains strong fluorescence emission while the light from the sodium fluorescein is quenched, allowing for the fluorescence detection of ATP with a detection limit as low as 0.735 nM. This method is simple, sensitive, and enables rapid and accurate specific quantitative detection of ATP, and can be widely used in the biomedical field.

The invention relates to a preparation method of an efficient fluorescent marker, which comprises the following steps: taking resorcinol and phthalic anhydride as starting materials, carrying out Friedel-Crafts acylation reaction in 80% sulfuric acid, carrying out Michael addition reaction, and dehydrating to generate fluorescein YYG-1; carrying out acylation reaction on the YYG-1 in acetic anhydride to obtain a diacetyl product YYG-2; hydrolyzing the YYG-2 with sodium methoxide to obtain fluorescein YYG-3; and carrying out alkalization and salification on the YYG-3 to prepare the sodium fluorescein YYG. According to the synthetic route, the high yield of the fluorescein sodium is realized by controlling the pH range in the hydrolysis step and the use amount of sodium hydroxide in the alkalization step, and the fluorescein sodium is high in purity, stable in quality and simple to operate.

The invention is a divisional application of which the application number is 2025116336025. The invention provides an application of ammonium glycyrrhizinate in preparation of a medicine for treating lacrimal gland secretion dysfunction related diseases, and relates to the technical field of biomedicine.A lacrimal gland dysfunction dry eye mouse model is constructed, and liposome-entrapped ammonium glycyrrhizinate is adopted for intervention, so that the risk of lacrimal gland secretion dysfunction related diseases is reduced, and the risk of lacrimal gland secretion dysfunction related diseases is reduced. Experimental results show that the ocular surface function and structure of a model animal are remarkably improved through ammonium glycyrrhizinate treatment, the corneal sodium fluorescein staining score of mice in an ammonium glycyrrhizinate treatment group is remarkably reduced, and corneal epithelium injury is effectively repaired; the epithelial structure in the cornea tissue tends to be complete, and inflammatory cell infiltration of a matrix layer is obviously relieved; the density of goblet cells in the conjunctiva is obviously increased, which indicates that the secretion function of the mucous layer on the ocular surface is recovered. And the ammonium glycyrrhizinate treatment has an improvement trend on tear film rupture time and tear secretion amount, which indicates that the ammonium glycyrrhizinate has a potential effect in the aspects of maintaining tear film stability and promoting basic tear secretion.