42 results about "Fluorescein sodium" patented technology

A novel fundus angiographic application and method using fluorescein isothiocyanate dextrans (FITC-dextran) as fluorescence angiographic dye for diagnosis and evaluation of eye diseases on humans is disclosed. The method involves the process of constitution of FITC-dextran solution and intravenous injection of the said solution, followed by observation and photography of ocular fundus circulation using a fluorescence fundus camera with an image system. The invention offers improved fundus angiograms over the traditional Na-fluorescein fundus angiography (Na-fluorescein) and indocyanine green fundus angiography (ICG) with the advantages of higher clarity and higher resolution; capable of visualizing both the retinal and the choroidal circulations systems simultaneously; providing longer fundus image duration so that it can be used as a directly mapping guidance in retinal surgery or to complete the angiograms for both eyes by a single process; providing more information about the pathological changes occurred among retinal, sub-retinal and choroidal circulation systems.

The invention discloses a method for measuring the active oxygen level in phanerochaete chrysosporium in treated wastewater. The method comprises the following steps: washing a phanerochaete chrysosporium bacterium ball in the treated wastewater, adding a liquid culture medium for further culture, further adding 2',7'-dichloro dihydro fluorescein sodium diacetate into the liquid culture medium after culture to obtain a mixture solution, carrying out incubation on the mixture solution, filtering, then performing ultrasonic crushing and centrifuging on the bacterium ball obtained through filtration, extracting a supernate, and finally measuring the fluorescence intensity of the oxidized dichlorofluorescein in the supernate. The method has the advantages that the operation condition is simple, the application is easy and the active oxygen level in the phanerochaete chrysosporium in the treated wastewater can be accurately and visibly measured.

The present invention relates to a colored perfusion liquid exclusively for ophthalmic surgery. The colored perfusion liquid exclusively for ophthalmic surgery of the present invention includes conventional ophthalmic balanced salt solution and is characterized in that the present invention further contains 0.001 to 0.1mg / ml fluorescein sodium. The present invention firstly proposes the intraocular colored perfusion liquid and a concept of colored surgery, the present invention has the advantages that: the present invention can be filled in the anterior chamber, vitreous body, lens capsular bag and other lacunas, and can form the contrast with the intraocular transparent and semi-permeable membrane tissues, such as, cornea, lens capsule membrane, lens, vitreous body, retina and so on, therefore, the operator can identify such structures easily and the tissues which are difficult to identify become visible, therefore, the surgery becomes easy, the operation is accurate, the time is shorten, the retina, lens capsule membrane and other main structures are protected, the surgical complications are reduced, and better surgical effects are achieved accordingly. In addition, the present invention changes and overcomes the problems that the past localized staining method is time-consuming and needs to rinse, the surgical process is complicated, the operation is fairly troublesome, andthe method can not carry out the differential display of the around tissues, that is the scope is too small etc.

The invention relates to a preparation method of high-purity fluorescein sodium. The preparation method comprises the following steps of: acetylating by using crude fluorescein sodium as the raw material and acetic anhydride as an acetylation reagent; recrystallizing the acetylated fluorescein; hydrolyzing; and salifying to obtain high-purity fluorescein sodium. In the preparation process of the obtained fluorescein sodium, the acetylation solvent is mild and can finish a reaction in a relatively short time without adding a catalyst so that the reaction cost is lower and the obtained product is safer.

The invention relates to an ultrasonic drug delivery device for SMILE lens dyeing low-temperature separation liquid. Wherein, the SMILE technique lens dyeing low-temperature separation liquid is kept at 0-4°C and is used for SMILE technique dyeing infiltration separation lens, including balanced salt solution and 0.005mg / ml~0.5mg / ml fluorescein sodium, balanced salt solution The ingredients include 0.29mg / ml~0.8mg / ml potassium chloride, 0.19mg / ml~0.5mg / ml calcium chloride, 0.25mg / ml~0.35mg / ml magnesium chloride, 0.3mg / ml~0.45mg / ml sodium acetate And 1.6mg / ml~2.2mg / ml sodium citrate. The low-temperature separation fluid for SMILE lens dyeing provided by this application can be dyed and infiltrated in the corneal cap (cap), the front surface of the corneal stroma lens (cap-lens layer, upper layer), and the back of the corneal stroma lens The surface (lens-posterior stroma layer, lower layer), corneal cap incision, etc., make the upper and lower surfaces of the corneal stroma lens that are difficult to distinguish visible, thereby reducing the difficulty of corneal lens separation operation, making the operation more accurate, shortening the time, and protecting the corneal cap And corneal stroma and other major structures, reducing surgical complications.

The invention discloses a rhodamine B fluorescent tracer automatic configuration and delivery control device and an operation method, including a water supply device, an integrated three-chamber combined box, a centralized control device, and an online real-time monitoring device; the integrated three-room combined box includes The premixing chamber, mixing chamber and storage chamber are connected in sequence; the premixing chamber is responsible for storing the dry powder of rhodamine, fluorescein sodium, and Tianlaibao; the mixing chamber includes a temperature sensor, a stirring rod, a cooling base, and a temperature sensor. The sensor is placed in the lower part of the mixing chamber; the stirring rod is used to stir the solution to be mixed; the cooling base is located at the bottom of the premixing chamber, the temperature sensor is connected to the centralized control device, and the centralized control device controls the cooling base to control the temperature in the mixing chamber; the storage room is used Store the prepared solution. The online real-time monitoring device is used to monitor various parameters of the prepared solution; the data is transmitted to the centralized control device, and the historical data is kept in time.

The invention discloses a preparation method of a double-fluorescence-labeled nano material. The preparation method comprises the following steps: connecting an intermediate substance CDI with HP-beta-CD; enabling HP-beta-CD-CDI and PEI to react in an organic solvent to prepare an HP-beta-CD-PEI solution; after dialysis, performing freeze-drying to obtain HP-beta-CD-PEI; under the catalysis of EDC(ethylene dichloride), coupling with rhodamine B to obtain an HP-beta-CD-PEI-RhB solution; after dialysis, performing freeze-drying to obtain HP-beta-CD-PEI-RhB; adsorbing fluorescein sodium throughthe HP-beta-CD-PEI-RhB; performing dialysis and freeze-drying to obtain the double-fluorescence-labeled nano material. The double-fluorescence-labeled nano material prepared by the method has the characteristics of non-radioactive performance, high stability, high sensitivity, high selectivity and the like; when being applied to researches of medicine activity, the double-fluorescence-labeled nanomaterial has no influence on the activity of medicines.

The invention relates to nanomagnetic beads of a coupled fluorescein and a preparation method thereof. The method comprises the following specific steps: mixing aniline with a hydrochloric acid solution, adding gamma-iron oxide in the mixed solution, stirring continuously, slowly adding aniline in ammonium persulfate, stirring again, performing suction filtering, washing filtrate with secondary water and ethanol until the filtrate is colorless, then doping with ammonia water to obtain polyaniline in an eigen state, performing suction filtering on the product, washing filtrate with secondary water until the filtrate is colorless, and performing vacuum drying to obtain gamma-iron oxide / polyaniline; and adding gamma-iron oxide / polyaniline and fluorescein sodium in a protonic acid solution, stirring, performing suction filtering, washing filtrate with water until the filtrate is colorless, and performing vacuum drying to obtain the nanomagnetic beads of the coupled fluorescein. By using the nanomagnetic beads for fluorescent tracing provided by the invention, the distribution of the nanomagnetic beads under a complicated internal environment in an animal experiment of the nanomagneticbeads can be detected; and in the further medical application stage, the nanomagnetic beads can be used for the tracing of a nanomagnetic bead medicament, thus the site of action and concentration ofthe medicine can be determined.