Fluid-enhanced dielectrophoretic single cell arrangement and control chip and making method thereof

Abstract

The invention belongs to the technical field of biological microfluidic chips. In order to solve the technical problems that the conventional single cell analysis chip is low in reliability, high in cost and difficult in single cell releases, the invention provides a fluid-enhanced dielectrophoretic single cell arrangement and control chip and a making method thereof. The whole chip adopts a sandwich structure, and sequentially comprises conduit connectors, an upper substrate, an upper-layer microelectrode, a microchannel, a microwall array, a lower-layer microelectrode and a lower substrate from top to bottom, wherein two through holes facilitating feeding and discharging of an experimental buffer solution are formed in the upper substrate; the conduit connectors are fixed to the through holes in the upper surface of the upper substrate; both the upper-layer microelectrode and the lower-layer microelectrode are interdigital electrodes; the upper-layer microelectrode and the lower-layer microelectrode are vertically intersected; the microwall array comprises micron-sized circular deep holes; and each microwall is positioned at the center of an intersection of the upper-layer microelectrode and the lower-layer microelectrode. The fluid-enhanced dielectrophoretic single cell arrangement and control chip solves the problems of low efficiency, low reliability, high cost and difficult single cell release, caused by principle singleness, of the conventional chip.

Description

technical field [0001] The invention belongs to the technical field of biological microfluidic chips, and in particular relates to a fluid-enhanced dielectrophoresis single-cell arrangement and control chip and a manufacturing method thereof. Background technique [0002] Cells are the most basic unit to form and maintain life. In recent years, the research on cells has greatly promoted the development of medical diagnosis, life science and other fields. Studies have shown that population cells in the same external environment have individual differences and cells in the mixed solution will affect each other. The research on population cells can only obtain statistical results, and individual differences will be ignored, resulting in rare abnormal cell signals (rare cells themselves) structural information and responses to external stimuli), and thus cannot obtain reliable relevant information. Therefore, the study of single cells is of great significance. The premise of c...

AI Technical Summary

Problems solved by technology

[0004] The present invention provides a fluid-enhanced dielectrophoresis single-cell arrangement and control chip and its manufacturing method to solve the technical problems of low reliability, high cost and difficulty in releasing single cells in the existing single-cell analysis chip

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